Autophagy in the intestinal epithelium regulates Citrobacter rodentium infection.

abstract：:Eleven hybridoma clones which secrete monoclonal antibodies against purified rat liver alcohol dehydrogenase (EC 1.1.1.1) were isolated. Antibodies (R-1-R-11) were identified by their ability to bind to immobilized pure alcohol dehydrogenase in an enzyme-linked immunoadsorbent assay, in which antibody R-9 showed the h...

journal_title：Archives of biochemistry and biophysics

authors： Lad PJ,Schenk DB,Leffert HL

更新日期：1984-12-01 00:00:00

abstract：:A ganglioside GM1-specific alpha 1-->2fucosyltransferase is induced during the early stages of chemical carcinogenesis with N-2-acetylaminofluorene (AAF) in rat liver hepatocytes. The induction of this enzyme gives rise to the expression of a fucose-containing ganglioside with the same determinant structure as blood g...

journal_title：Archives of biochemistry and biophysics

authors： Sherwood AL,Holmes EH

更新日期：1998-07-15 00:00:00

abstract：:Antimicrobial and cell-penetrating peptides have been the object of extensive studies for more than 60 years. Initially these two families were studied separately, and more recently parallels have been drawn. These studies have given rise to numerous methodological developments both in terms of observation techniques ...

journal_title：Archives of biochemistry and biophysics

authors： Emmanuelle S,Astrid W,Sandrine S,Sophie C,Nicolas R

更新日期：2021-01-07 00:00:00

abstract：:Epidemiological evidence strongly suggests that lycopene consumption contributes to prostate cancer risk reduction. Preclinical studies show that lycopene acts via different mechanisms, which have the potential to cooperate in reducing the proliferation of normal and cancerous prostate epithelial cells, in reducing DN...

journal_title：Archives of biochemistry and biophysics

authors： Wertz K,Siler U,Goralczyk R

更新日期：2004-10-01 00:00:00

abstract：:A full-length recombinant mutant of diphtheria toxin containing serine in place of a crucial active-site glutamate has been purified and characterized. The serine substitution caused a minor structural alteration in the toxin as measured by trypsinolysis. ADP-ribosyltransferase activity and cytotoxicity of the mutant ...

journal_title：Archives of biochemistry and biophysics

authors： O'Keefe DO

更新日期：1992-08-01 00:00:00

abstract：:The kinetics of bovine Cu,Zn superoxide dismutase were studied by pulse radiolysis. To ensure the absence of catalytically active free copper, commercially obtained holo-superoxide dismutase was demetallated, and the apo-superoxide dismutase concentrations were determined by isothermal titration calorimetry prior to r...

journal_title：Archives of biochemistry and biophysics

authors： Michel E,Nauser T,Sutter B,Bounds PL,Koppenol WH

更新日期：2005-07-15 00:00:00

abstract：:Studies of the response to hydrogen peroxide as well as the characterization of small, noncoding RNAs and small proteins of less than 50 amino acids in Escherichia coli have given new perspectives on redox sensing, the nature of regulators and gene organization that are relevant to all organisms. ...

journal_title：Archives of biochemistry and biophysics

authors： Storz G

更新日期：2016-04-01 00:00:00

abstract：:Because angiogenesis underlies the pathogenesis of numerous conditions (cancer, psoriasis, macular degeneration), there is a pressing need for continued investigations into angiogenic signaling and potential drug targets. Antiangiogenic agents can be classified as either direct or indirect. Direct antiangiogenics act ...

journal_title：Archives of biochemistry and biophysics

authors： Berrios RL,Arbiser JL

更新日期：2011-04-15 00:00:00

abstract：:A 42-kDa plant outer mitochondrial membrane protein, MOM42, has been identified as an essential component of the plant mitochondrial precursor protein translocation apparatus. Immunological cross-reactivity has been detected between antibodies raised against both Neurospora and yeast mitochondrial outer membrane prote...

journal_title：Archives of biochemistry and biophysics

authors： Perryman RA,Mooney B,Harmey MA

更新日期：1995-02-01 00:00:00

abstract：:The rotating frame nuclear magnetic resonance relaxation rate R(1rho) in the blood and cell lysate was studied at 4.7T to provide reference values for in vivo modeling and to address the mechanisms contributing to net relaxation. A strong dependence on oxygenation, hematocrit, and spin lock field strength B(1) (0.2-1....

journal_title：Archives of biochemistry and biophysics

authors： Johanna Silvennoinen M,Kettunen MI,Clingman CS,Kauppinen RA

更新日期：2002-09-01 00:00:00

abstract：:The NCI-H69 cell alpha 1----3fucosyltransferase has been purified from a 0.2% Triton X-100R solubilized enzyme fraction by GDP-hexanolamine-Sepharose affinity chromatography and Superose 12 gel filtration. Photoaffinity labeling experiments with 125I-GDP-hexanolaminyl-4-azidosalicylic acid present in concentrations eq...

journal_title：Archives of biochemistry and biophysics

authors： Holmes EH

更新日期：1992-08-01 00:00:00

abstract：:Several experiments were carried out to study the difference between two isozymes (alpha(+) and alpha) of (Na+ + K+)-ATPase in the conformational equilibrium. Rat brain (Na+ + K+)-ATPase was much more thermolabile than the kidney enzyme. Both enzymes were protected from heat inactivation not only by Na+ and K+, but al...

journal_title：Archives of biochemistry and biophysics

authors： Matsuda T,Iwata H

更新日期：1988-06-01 00:00:00

abstract：:The importance of the sn-glycerol- 3-phosphate (G-3-P) electron transfer shuttle in hormonal regulation of gluconeogenesis was examined in hepatocytes from rats with decreased mitochondrial G-3-P dehydrogenase activity (thyroidectomized) or increased G-3-P dehydrogenase activity [triiodothyronine (T(3)) or dehydroepia...

journal_title：Archives of biochemistry and biophysics

authors： Kneer N,Lardy H

更新日期：2000-03-01 00:00:00

abstract：:The cellular factor, papillomavirus (PV)-binding factor (PBF)/Huntington's disease binding protein 2 (HDBP2), was identified by its ability to bind regulatory sequences of certain papillomavirus types as well as the Huntington's disease gene. PBF is thought to be a novel nuclear-shuttling transcription factor with unk...

journal_title：Archives of biochemistry and biophysics

authors： Sichtig N,Silling S,Steger G

更新日期：2007-08-01 00:00:00

abstract：:Cytochromes P450 are enzymes that catalyse the oxidation of a wide variety of compounds that range from small volatile compounds, such as monoterpenes to larger compounds like steroids. These enzymes can be modified to selectively oxidise substrates of interest, thereby making them attractive for applications in the b...

journal_title：Archives of biochemistry and biophysics

authors： Stok JE,Giang PD,Wong SH,De Voss JJ

更新日期：2019-09-15 00:00:00

abstract：:A full-length cDNA for human placental thromboxane synthase and a shortened cDNA lacking the sequence corresponding to the N-terminal 2-29 amino acids were expressed in Escherichia coli using a pCW expression vector. Both intact and truncated recombinant enzyme were found in the membrane fraction and were catalyticall...

journal_title：Archives of biochemistry and biophysics

authors： Xia Z,Tai HH

更新日期：1995-08-20 00:00:00

abstract：:NADP-malate dehydrogenase activity, the ratio of NADPH to NADP, and thioredoxin redox state in Zea mays chloroplasts were determined after various treatments. Following transfer from dark to light, NADP-malate dehydrogenase was activated more than 20-fold within 10 min while the proportion of pyridine nucleotide as NA...

journal_title：Archives of biochemistry and biophysics

authors： Rebeille F,Hatch MD

更新日期：1986-08-15 00:00:00

abstract：:The modulation of the plasma membrane calcium pump (PMCA) gene expression is not well understood. We now present evidence that PMCA mRNA is induced by various hormones or agonists via multiple second messenger pathways that include protein kinase C-, cAMP-, and Ca(2+)-dependent mechanisms. Among several types of cultu...

journal_title：Archives of biochemistry and biophysics

authors： Kuo TH,Liu BF,Diglio C,Tsang W

更新日期：1993-09-01 00:00:00

abstract：:Ex vivo protein modifications occur within plasma and serum (P/S) samples due to prolonged exposure to the thawed state-which includes temperatures above -30 °C. Herein, the ex vivo glycation of human serum albumin from healthy and diabetic subjects was monitored in P/S samples stored for hours to months at -80 °C, -2...

journal_title：Archives of biochemistry and biophysics

authors： Jeffs JW,Ferdosi S,Yassine HN,Borges CR

更新日期：2017-09-01 00:00:00

abstract：:The gene p8 encodes for a small cytoprotective protein with no apparent enzymatic activity being proposed to act as co-transcription factor whose expression is increased during inflammation. Recent data from astrocytes demonstrates that p8 suppression leads to induction of heme oxygenase 1 (HO-1). Here, we assessed th...

journal_title：Archives of biochemistry and biophysics

authors： Weis S,Bielow T,Sommerer I,Iovanna J,Malicet C,Mössner J,Hoffmeister A

更新日期：2015-01-01 00:00:00

abstract：:Triacylglycerol plays a critical role in an organism's ability to withstand fuel deprivation, and dysregulation of triacylglycerol synthesis is important in the development of diseases such as obesity and diabetes. Glycerol-3-phosphate acyltransferase (GPAT) catalyzes the initial and committed step of glycerolipid syn...

journal_title：Archives of biochemistry and biophysics

authors： Lewin TM,Granger DA,Kim JH,Coleman RA

更新日期：2001-12-01 00:00:00

abstract：:The structure of rabbit muscle pyruvate kinase crystallized as a complex with Mg2+, K+, and L-phospholactate (L-P-lactate) has been solved and refined to 2.7 A resolution. The crystals, grown from solutions of polyethylene glycol 8000 at pH 7.5, belong to the space group P2(1) and have unit cell parameters a = 144.4 A...

journal_title：Archives of biochemistry and biophysics

authors： Larsen TM,Benning MM,Wesenberg GE,Rayment I,Reed GH

更新日期：1997-09-15 00:00:00

abstract：:1-O-Alkyl-2-acetyl-sn-glycero-3-phosphocholine (AGEPC) induced phosphorylation of two proteins having molecular masses of approximately 20- and 40-kDa in washed rabbit platelets in a concentration- and time-dependent manner. Sequential stimulation with AGEPC did not induce additional protein phosphorylation, supportin...

journal_title：Archives of biochemistry and biophysics

authors： Sugatani J,Hanahan DJ

更新日期：1986-05-01 00:00:00

abstract：:Substrate specificity of human mitochondrial low Km aldehyde dehydrogenase (EC 1.2.1.3) E2 isozyme has been investigated employing p-nitrophenyl esters of acyl groups of two to six carbon atoms and comparing with that of aldehydes of one to eight carbon atoms. The esterase reaction was studied under three conditions: ...

journal_title：Archives of biochemistry and biophysics

authors： Mukerjee N,Pietruszko R

更新日期：1992-11-15 00:00:00

abstract：:Thromboxane A2 (TXA) is a potent vasoconstrictor and mediator of platelet aggregation. Thromboxane synthase (TXAS), which catalyzes the biosynthesis of TXA, is a member of the cytochrome P450 superfamily. We report here the complete genomic structure of the human TXAS gene. The gene contains 13 exons and is 193 kb lon...

journal_title：Archives of biochemistry and biophysics

authors： Tazawa R,Green ED,Ohashi K,Wu KK,Wang LH

更新日期：1996-10-15 00:00:00

abstract：:Sucrose-phosphate synthase (SPS; EC 2.4.1.14) is regulated in part by reversible protein phosphorylation. When dephospho-SPS is partially purified from illuminated spinach leaves and incubated with [gamma-32P]ATP the enzyme is phosphorylated by a copurifying protein kinase. In this report, 32P-phosphopeptides from try...

journal_title：Archives of biochemistry and biophysics

authors： McMichael RW Jr,Klein RR,Salvucci ME,Huber SC

更新日期：1993-12-01 00:00:00

abstract：:The sesquiterpene cyclase, aristolochene synthase, has been purified from Penicillium roqueforti by gel filtration and anion-exchange chromatography. Isolation was facilitated by a change in the elution behavior of the enzyme during gel filtration at different steps in the purification. The purified enzyme had a speci...

journal_title：Archives of biochemistry and biophysics

authors： Hohn TM,Plattner RD

更新日期：1989-07-01 00:00:00

abstract：:Properly regulated cyclin proteolysis is critical for normal cell cycle progression. A nine-amino acid peptide motif called the destruction box (D box) is present at the N terminus of the yeast mitotic cyclins. This short sequence is required for cyclin ubiquitination and subsequent proteolysis. The anaphase-promoting...

journal_title：Archives of biochemistry and biophysics

authors： Meyn MA 3rd,Melloy PG,Li J,Holloway SL

更新日期：2002-11-15 00:00:00

abstract：:According to differences in mobility on SDS-polyacrylamide gel electrophoresis, calpastatins (inhibitor proteins of the calcium-dependent proteinase calpain) are classified into the tissue type (100-120 kDa) and the erythrocyte type (70 kDa), which lacks the amino-terminal domains (domains L and 1). We investigated th...

journal_title：Archives of biochemistry and biophysics

authors： Takano E,Nosaka T,Lee WJ,Nakamura K,Takahashi T,Funaki M,Okada H,Hatanaka M,Maki M

更新日期：1993-06-01 00:00:00

abstract：:Tetrahydro-dUMP, an analog of the putative transition state in aminohydrolysis of deoxycytidine monophosphate (dCMP) inhibits the allosteric enzyme deoxycytidylate aminohydrolase with high affinity. The inhibition is reversible, and its kinetics is consistent with the analog binding at the substrate site only to one a...

journal_title：Archives of biochemistry and biophysics

authors： Nucci R,Febbraio F,Piccialli G,de Napoli L,Vaccaro C,Rossi M,Whitehead EP

更新日期：1994-04-01 00:00:00