Recombinant human erythropoietin: purification and analysis of carbohydrate linkage.

abstract：:The binding site for ATP to tubulin was established by use of the photoaffinity label [gamma-32P]N3ATP. Photolysis of the analog in the presence of tubulin resulted in covalent modification of the protein as revealed by autoradiography of electropherograms. Scanning the autoradiograms showed that the ATP analog was bo...

journal_title：Archives of biochemistry and biophysics

authors： Zabrecky JR,Cole RD

更新日期：1983-09-01 00:00:00

abstract：:Piratoxins (PrTX) I and III are phospholipases A2 (PLA2s) or PLA2 homologue myotoxins isolated from Bothrops pirajai snake venom, which also induce myonecrosis, bactericidal activity against Escherichia coli, disruption of artificial membranes, and edema. PrTX-III is a catalytically active hemolytic and anticoagulant ...

journal_title：Archives of biochemistry and biophysics

authors： Soares AM,Andrião-Escarso SH,Bortoleto RK,Rodrigues-Simioni L,Arni RK,Ward RJ,Gutiérrez JM,Giglio JR

更新日期：2001-03-15 00:00:00

abstract：:Rabbit anti-tyrosinase antibodies were used to study the abundance, processing, and degradation of tyrosinase in murine (Cloudman) melanoma cells. The polyclonal antibodies precipitated low-molecular-weight (68,000 and 70,000) and high-molecular-weight (78,000 and 80,000) tyrosinases that had a precursor-product relat...

journal_title：Archives of biochemistry and biophysics

authors： Halaban R,Pomerantz SH,Marshall S,Lerner AB

更新日期：1984-04-01 00:00:00

abstract：:It was shown previously that the ATP synthase complex of bovine heart mitochondria contains an essential set of thiols or dithiols in its membrane sector (F0), whose modification by various reagents results in uncoupling [Yagi, T., and Hatefi, Y. (1984) Biochemistry 23, 2449-2455]. The sensitivity to modifiers was inc...

journal_title：Archives of biochemistry and biophysics

authors： Yagi T,Hatefi Y

更新日期：1987-04-01 00:00:00

abstract：:The in vivo binding of platinum to metallothionein (MT) has been observed in rat tissues following injections of the cis and trans isomers of DDP (dichlorodiammineplatinum(II]. Platinum in either cis-DDP or trans-DDP does not directly induce MT; platinum-MT is produced by the replacement of previously bound zinc in th...

journal_title：Archives of biochemistry and biophysics

authors： Zelazowski AJ,Garvey JS,Hoeschele JD

更新日期：1984-02-15 00:00:00

abstract：:Rat liver microsomal glutathione transferase displays glutathione peroxidase activity with linoleic acid hydroperoxide, linoleic acid ethyl ester hydroperoxide, and dilinoleoyl phosphatidylcholine hydroperoxide, with rates of 0.2, 0.3, and 0.3 mumol/min/mg, respectively. The activities are increased between three- and...

journal_title：Archives of biochemistry and biophysics

authors： Mosialou E,Morgenstern R

更新日期：1989-11-15 00:00:00

abstract：:Hyaluronidase from fresh human serum was purified to apparent homogeneity in a two-step procedure. Potent serum inhibitors of hyaluronidase activity were removed during the course of the purification. Isolation of the enzyme was expedited by the use of a newly devised ELISA-like assay. Enzyme activity was measured by ...

journal_title：Archives of biochemistry and biophysics

authors： Afify AM,Stern M,Guntenhöner M,Stern R

更新日期：1993-09-01 00:00:00

abstract：:While prior studies focusing on male mice suggest a role for sterol carrier protein-2/sterol carrier protein-x (SCP-2/SCP-x; DKO) on hepatic phytol metabolism, its role in females is unresolved. This issue was addressed using female and male wild-type (WT) and DKO mice fed a phytoestrogen-free diet without or with 0.5...

journal_title：Archives of biochemistry and biophysics

authors： McIntosh AL,Storey SM,Huang H,Kier AB,Schroeder F

更新日期：2017-12-01 00:00:00

abstract：:Peroxynitrite-mediated one-electron oxidations may be an important event in its cytotoxic mechanisms, and yet, free radical formation in the presence of peroxynitrite is difficult to study by EPR-spin trapping because adducts from most spin traps are destroyed by the oxidant. This led to some controversy with regard t...

journal_title：Archives of biochemistry and biophysics

authors： Gatti RM,Alvarez B,Vasquez-Vivar J,Radi R,Augusto O

更新日期：1998-01-01 00:00:00

abstract：:The interactions between various dietary cancer chemopreventive phytochemicals in drug transporter functions are not well studied. In this study, the effects of genistein and resveratrol on the multidrug resistance protein 2 (MRP2) expression and the underlying molecular mechanisms were investigated using HepG2-C3 cel...

journal_title：Archives of biochemistry and biophysics

authors： Kim JH,Chen C,Tony Kong AN

更新日期：2011-08-15 00:00:00

abstract：:The short term response of the L-6 cell line of rat skeletal myoblasts to elevated extracellular iron concentrations was studied. It was found in all cases that iron as the nitrilotriacetate (NTA) chelate was effective at donating iron to the cells and at stimulating ferritin synthesis. After 48 h in 50 microM ferric ...

journal_title：Archives of biochemistry and biophysics

authors： Rittling SR,Woodworth RC

更新日期：1983-08-01 00:00:00

abstract：:The inborn deficiency of adenosine deaminase is characterised by accumulation of excess amounts of cytotoxic deoxyadenine nucleotides in lymphocytes. Formation of dATP requires phosphorylation of deoxyadenosine by deoxycytidine kinase (dCK), the main nucleoside salvage enzyme in lymphoid cells. Activation of dCK by a ...

journal_title：Archives of biochemistry and biophysics

authors： Keszler G,Virga S,Spasokoukotskaja T,Bauer PI,Sasvari-Szekely M,Staub M

更新日期：2005-04-01 00:00:00

abstract：:The single cysteine residue of human serum albumin (HSA-SH) is the most abundant plasma thiol. HSA transports fatty acids (FA), a cargo that increases under conditions of diabetes, exercise or adrenergic stimulation. The stearic acid-HSA (5/1) complex reacted sixfold faster than FA-free HSA at pH 7.4 with the disulfid...

journal_title：Archives of biochemistry and biophysics

authors： Torres MJ,Turell L,Botti H,Antmann L,Carballal S,Ferrer-Sueta G,Radi R,Alvarez B

更新日期：2012-05-01 00:00:00

abstract：:The modification of reactive protein sulfhydryls by S-nitrosoglutathione and other NO donors has been studied by gel isoelectric focusing. S-nitrosylated, unmodified, and S-glutathiolated protein forms are differentiated by this method. With specific antibodies for the protein of interest, both S-nitrosylation and S-g...

journal_title：Archives of biochemistry and biophysics

authors： Ji Y,Akerboom TP,Sies H,Thomas JA

更新日期：1999-02-01 00:00:00

abstract：:Experimental autoimmune encephalomyelitis (EAE) is a commonly used mouse model of multiple sclerosis, a chronic inflammatory disease of the central nervous system (CNS) characterized by demyelination leading to brain and spinal cord malfunctions. We postulate that not only biological but also biomechanical properties ...

journal_title：Archives of biochemistry and biophysics

authors： Pyka-Fościak G,Zemła J,Lis GJ,Litwin JA,Lekka M

更新日期：2020-02-15 00:00:00

abstract：:Asthma is characterized by a predominant T(H)2 type immune response to airborne allergens. Controlling T(H)2 cell function has been proposed as therapy for this disease. We show here that ligands for the nuclear receptor peroxisome proliferator activated receptor (PPAR)gamma significantly reduced the immunological sym...

journal_title：Archives of biochemistry and biophysics

authors： Mueller C,Weaver V,Vanden Heuvel JP,August A,Cantorna MT

更新日期：2003-10-15 00:00:00

abstract：:Salicylhydroxamic acid reductase, which catalyzes the reduction of salicylhydroxamic acid to salicylamide, was purified from rat liver cytosol. The purification procedure consisted of fractionation with ammonium sulfate, chromatography with Phenyl-Toyopearl 650, DEAE-cellulose, hydroxyapatite, and Sephadex G-200, and ...

journal_title：Archives of biochemistry and biophysics

authors： Katsura H,Kitamura S,Tatsumi K

更新日期：1993-05-01 00:00:00

abstract：:Osmolytes are small organic molecules accumulated by organisms under stress conditions to protect macromolecular structure and function. In the present study, we have investigated the effect of several binary osmolyte mixtures on the protein folding/stability and function of RNase-A. For this, we have measured ΔGD(o) ...

journal_title：Archives of biochemistry and biophysics

authors： Warepam M,Singh LR

更新日期：2015-05-01 00:00:00

abstract：:Peroxynitrite, a biological oxidant formed from the reaction of nitric oxide with the superoxide radical, is associated with many pathologies, including neurodegenerative diseases, such as multiple sclerosis (MS). Gout (hyperuricemic) and MS are almost mutually exclusive, and uric acid has therapeutic effects in mice ...

journal_title：Archives of biochemistry and biophysics

authors： Squadrito GL,Cueto R,Splenser AE,Valavanidis A,Zhang H,Uppu RM,Pryor WA

更新日期：2000-04-15 00:00:00

abstract：:Human soluble epoxide hydrolase (hsEH) has been shown to play a role in regulating blood pressure and inflammation. HsEH consists of an N-terminal phosphatase and a C-terminal epoxide hydrolase domain. In the present study, we examined the effects of polymorphisms in the hsEH gene on phosphatase activity, enzyme stabi...

journal_title：Archives of biochemistry and biophysics

authors： Srivastava PK,Sharma VK,Kalonia DS,Grant DF

更新日期：2004-07-15 00:00:00

abstract：:Incubation of either Chlorella nitrate reductase or the recombinant flavin domain of spinach nitrate reductase with reagents specific for modification of cysteine residues, such as N-ethylmaleimide, resulted in a time-dependent inactivation of NADH:ferricyanide reductase activity which could be prevented by incubation...

journal_title：Archives of biochemistry and biophysics

authors： Trimboli AJ,Quinn GB,Smith ET,Barber MJ

更新日期：1996-07-01 00:00:00

abstract：:Mycobactin acylation plays a crucial role in the ability of Mycobacterium tuberculosis to acquire intracellular iron during infection. M. tuberculosis Rv1347c, the lysine N(epsilon)-acyltransferase responsible for mycobactin acylation, represents a valid target for the development of novel anti-tubercular agents. Here...

journal_title：Archives of biochemistry and biophysics

authors： Frankel BA,Blanchard JS

更新日期：2008-09-15 00:00:00

abstract：:Archaeal replication machinery represents a core version of this in eukaryotes. The crenarchaeon Sulfolobus solfataricus has the potential to be a powerful model system to understand the central mechanism of eukaryotic DNA replication because it contains three active origins of replication and three eukaryote-like Orc...

journal_title：Archives of biochemistry and biophysics

authors： He ZG,Feng Y,Wang J,Jiang PX

更新日期：2008-03-15 00:00:00

abstract：:The paper reports a study on the reactions of grass pea (Lathyrus sativus) amine oxidase (GPAO) with several aliphatic diamines. The influence of the chain length and of unsaturations in the molecules was examined. Kinetic measurements confirmed that trans-, i.e., (E)-2-butene-1,4-diamine (TDABE) and cis-, i.e., (Z)-2...

journal_title：Archives of biochemistry and biophysics

authors： Sebela M,Frébort I,Lemr K,Brauner F,Pec P

更新日期：2000-12-01 00:00:00

abstract：:The present paper focussed on the mechanistic aspect of the reaction of singlet oxygen (1O2), in its lowest excited state (1 Delta g), toward DNA constituents. It is well known that 1O2 is able to react with the guanine moiety of DNA to produce almost specifically 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo). Howev...

journal_title：Archives of biochemistry and biophysics

authors： Ravanat JL,Martinez GR,Medeiros MH,Di Mascio P,Cadet J

更新日期：2004-03-01 00:00:00

abstract：:Cancer cells exhibit extreme sensitivity to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) over normal cells, highlighting TRAIL's potential as a novel and effective cancer drug. However, the therapeutic effect of TRAIL is limited due to drug resistance. In the present study, we sought to investigate ...

journal_title：Archives of biochemistry and biophysics

authors： Wu B,Xiong J,Zhou Y,Wu Y,Song Y,Wang N,Chen L,Zhang J

更新日期：2020-10-15 00:00:00

abstract：:The enzyme phenylalanine hydroxylase catalyzes the hydroxylation of excess phenylalanine in the liver to tyrosine. The enzyme is regulated allosterically by phenylalanine and by phosphorylation of Ser16. Hydrogen/deuterium exchange monitored by mass spectrometry has been used to gain insight into any structural change...

journal_title：Archives of biochemistry and biophysics

authors： Li J,Fitzpatrick PF

更新日期：2013-07-15 00:00:00

abstract：:Three distinct acid phosphatases were recently reported in avian breast muscle [J. H. Baxter and C. H. Suelter (1984) Arch. Biochem. Biophys. 228, 397-406]. Of the increased acid phosphatase activity in dystrophic muscle compared to normal muscle, 84% can be accounted for as a low-molecular-weight, cytosolic form. Thi...

journal_title：Archives of biochemistry and biophysics

authors： Baxter JH,Suelter CH

更新日期：1985-05-15 00:00:00

abstract：:Chloride ion is found to be an essential factor in photoreactivation of the oxygen-evolving center. Tris-washed, 2,6-dichlorophenol indophenol-treated grana are low in both Mn content and oxygen-evolving activity. These grana can restore high oxygen-evolving activity, however, by incorporating Mn2+ ion under weak ligh...

journal_title：Archives of biochemistry and biophysics

authors： Yamashita T,Ashizawa A

更新日期：1985-05-01 00:00:00

abstract：:Upon treatment of Papaveracea cells with fungal elicitors, the biosynthesis of benzo[c]phenanthridine alkaloids is induced. Dihydrobenzophenanthridine oxidase, which catalyzes a later step in the biogenesis of these alkaloids, is one of the enzymes whose activity is elevated in the process. Here we report the 211-fold...

journal_title：Archives of biochemistry and biophysics

authors： Arakawa H,Clark WG,Psenak M,Coscia CJ

更新日期：1992-11-15 00:00:00