The role of chloride ion on the photoreactivation of the oxygen-evolving center of tris-washed, 2,6-dichlorophenol indophenol-treated grana.

abstract：:Saccharomyces cerevisiae phosphoenolpyruvate carboxykinase [ATP:oxaloacetate carboxy-lyase (transphosphorylating), EC 4.1.1.49] is completely inactivated by the 2',3'-dialdehyde derivative of ATP (oATP) in the presence of Mn2+. The dependence of the pseudo-first-order rate constant on reagent concentration indicates t...

journal_title：Archives of biochemistry and biophysics

authors： Saavedra C,Araneda S,Cardemil E

更新日期：1988-11-15 00:00:00

abstract：:A new plant lectin from elderberry (Sambucus nigra L.) bark, which was shown by immunochemical techniques to bind specifically to terminal Neu5Ac(alpha 2-6)Gal/GalNAc residues of glycoconjugates, was immobilized onto Sepharose 4B (SNA-Sepharose) and its carbohydrate binding properties was determined using a series of ...

journal_title：Archives of biochemistry and biophysics

authors： Shibuya N,Goldstein IJ,Broekaert WF,Nsimba-Lubaki M,Peeters B,Peumans WJ

更新日期：1987-04-01 00:00:00

abstract：:Three isoenzymes of UDP-glucuronyltransferase (UDPGT) have been separated and purified from liver microsomes of untreated female rats or female rats pretreated with 3-methylcholanthrene. The UDPGT isoenzymes were purified utilizing Chromatofocusing, column isoelectric focusing, and UDP-hexanolamine Sepharose 4B affini...

journal_title：Archives of biochemistry and biophysics

authors： Falany CN,Tephly TR

更新日期：1983-11-01 00:00:00

abstract：:Carbamoyl phosphate synthetase I (CPSase I) catalyzes the first reaction of the urea cycle in mammalian ureotelic species. The positive allosteric cofactor N-acetyl-L-glutamate (AGA) is required for CPSase I activity and is important for regulation of the urea cycle. A similar enzyme, CPSase III, catalyzes this reacti...

journal_title：Archives of biochemistry and biophysics

authors： Julsrud EA,Walsh PJ,Anderson PM

更新日期：1998-02-01 00:00:00

abstract：:Forskolin, a diterpene that exerts several pharmacological effects, activates adenylate cyclase in brain and in some other mammalian tissues. Properties of forskolin activation of adenylate cyclase from central nervous system of the dipterous Ceratitis capitata are described. The interaction of forskolin with the inse...

journal_title：Archives of biochemistry and biophysics

authors： Guillen A,Haro A,Municio AM

更新日期：1987-04-01 00:00:00

abstract：:The kinetic properties of UDP-glucuronosyltransferase were measured after brief treatment of liver microsomes at alkaline pH, followed by assay with p-nitro-phenol as aglycone, at pH 7.5. Enzyme activity increased in a graded fashion as the pH of pretreatment was increased above 8.0, with apparent maximal activation o...

journal_title：Archives of biochemistry and biophysics

authors： Dannenberg A,Wong T,Zakim D

更新日期：1990-03-01 00:00:00

abstract：:Two N-terminal fusion proteins combining Escherichia coli maltose-binding protein (MBP) and the 12-transmembrane-segment pBR322 tetracycline resistance protein (Tet) have been constructed to determine the strength and location of topology control signals within the N-terminal portion of the Tet protein. The fusions co...

journal_title：Archives of biochemistry and biophysics

authors： Miller KW,Jewell JE

更新日期：1995-10-01 00:00:00

abstract：:A cDNA was cloned from Ruta graveolens cells encoding a novel O-methyltransferase (OMT) with high similarity to orcinol or chavicol/eugenol OMTs, but containing a serine-rich N-terminus and a 13 amino acid insertion between motifs IV and V. Expression in Escherichia coli revealed S-adenosyl-l-methionine-dependent OMT ...

journal_title：Archives of biochemistry and biophysics

authors： Burga L,Wellmann F,Lukacin R,Witte S,Schwab W,Schröder J,Matern U

更新日期：2005-08-01 00:00:00

abstract：:Two novel medicinally important legume lectins from Bauhinia purpurea (BPL) and Wisteria floribunda (WFL) possessing extended sugar binding site were investigated for functional and conformational transitions using biochemical and biophysical techniques as well as bioinformatical tools. Homology model of BPL was const...

journal_title：Archives of biochemistry and biophysics

authors： Agrawal SB,Ghosh D,Gaikwad SM

更新日期：2019-02-15 00:00:00

abstract：:The so-called 90,000-Da heat shock protein (hsp90) from chicken liver has been purified and physically characterized in the presence of high levels of the serine phosphatase inhibitor fluoride. The protein is an elongated dimer with a molecular weight of 160,000 and a frictional ratio of 1.6. On two-dimensional electr...

journal_title：Archives of biochemistry and biophysics

authors： Iannotti AM,Rabideau DA,Dougherty JJ

更新日期：1988-07-01 00:00:00

abstract：:Under homeostatic conditions, Nrf2 activity is constitutively repressed. This process is dependent on Keap1, to which Nrf2 binds through the Neh2 domain. Since the N-terminal subdomain of Neh2 (Neh2-NT) contains evolutionarily conserved motifs, we examined the roles they play in the degradation of Nrf2. In Neh2-NT, we...

journal_title：Archives of biochemistry and biophysics

authors： Katoh Y,Iida K,Kang MI,Kobayashi A,Mizukami M,Tong KI,McMahon M,Hayes JD,Itoh K,Yamamoto M

更新日期：2005-01-15 00:00:00

abstract：:A microsomal preparation from seedlings of Zea mays catalyzed the NAD+-dependent oxidative decarboxylation of several substrates, including 4alpha-carboxy-cholest-7-en-3beta-ol, synthesized according to a new procedure, giving the first in vitro evidence for this enzymatic activity in a higher plant. A GC assay has be...

journal_title：Archives of biochemistry and biophysics

authors： Rondet S,Taton M,Rahier A

更新日期：1999-06-15 00:00:00

abstract：:Affinity columns prepared by immobilizing monoclonal antibodies that specifically recognize the Lea or the Leb blood group antigens can be used for analytical or preparative isolation of oligosaccharides with the corresponding reactivities. The number of immobilized functional antibody combining sites on a column and ...

journal_title：Archives of biochemistry and biophysics

authors： Dakour J,Lundblad A,Zopf D

更新日期：1988-07-01 00:00:00

abstract：:Homogeneous cultures of epithelial, endothelial, and mesangial cells from calf glomeruli were radiolabeled with [35S]sulfate in order to evaluate their capacity for the biosynthesis of the proteoglycan (PG) components present in the glomerular extracellular matrix. Although each cell type was observed to incorporate i...

journal_title：Archives of biochemistry and biophysics

authors： Shen GQ,Kresbach G,Spiro MJ,Spiro RG

更新日期：1995-08-01 00:00:00

abstract：:Spontaneously beating isolated rat atria were subjected to 1 h of anoxia at 37 degrees C in various cardioplegic solutions. Contraction continued for different times upon initiation of anoxia, depending on the nature of the cardioplegic solution. Two hundred micromolar P1,P5-di(adenosine-5')pentaphosphate (Ap5A) stopp...

journal_title：Archives of biochemistry and biophysics

authors： Savabi F,Bessman SP

更新日期：1986-07-01 00:00:00

abstract：:Methionine is oxidized to methionine sulfoxide by rat liver and kidney microsomes in an O2- and NADPH-dependent manner. In all microsomal assays, no methionine sulfone was detected. Use of a monoclonal antibody to rat liver cytochrome P-450 reductase, various cytochrome P-450 and peroxidase inhibitors, antioxidants, a...

journal_title：Archives of biochemistry and biophysics

authors： Krause RJ,Ripp SL,Sausen PJ,Overby LH,Philpot RM,Elfarra AA

更新日期：1996-09-01 00:00:00

abstract：:Aldehyde oxidase (AO) and xanthine oxidase (XO) are cytosolic enzymes that have been involved in some pathological conditions and play an important role in the biotransformation of drugs and xenobiotics. The increasing interest in these enzymes demands for a simple and rapid procedure for their purification. This pape...

journal_title：Archives of biochemistry and biophysics

authors： Maia L,Mira L

更新日期：2002-04-01 00:00:00

abstract：:A large number of mutations affecting the F(0) sector of Escherichia coli F(1)F(0) ATP synthase have been constructed and characterized. A subset of the missense mutations resulted in fully assembled enzyme complexes blocked in proton translocation and displaying marked decreases in ATP hydrolysis activity. The cataly...

journal_title：Archives of biochemistry and biophysics

authors： Gardner JL,Cain BD

更新日期：2000-08-01 00:00:00

abstract：:Previous work has shown that inhibitors of protein or mRNA synthesis block endonuclease activation in thymocytes undergoing programmed cell death. In the present study we used isolated nuclei to investigate the effects of cycloheximide and actinomycin D, inhibitors of protein and mRNA synthesis, respectively, on endog...

journal_title：Archives of biochemistry and biophysics

authors： McConkey DJ,Hartzell P,Orrenius S

更新日期：1990-04-01 00:00:00

abstract：:We tested the effects of a series of synthetic oligonucleotides (hybridons) complementary to the 5' noncoding and coding regions of rabbit beta-globin mRNA on endogenous protein synthesis in a rabbit reticulocyte cell-free translation system. With highly purified hybridons inhibition was completely specific for beta-g...

journal_title：Archives of biochemistry and biophysics

authors： Goodchild J,Carroll E 3rd,Greenberg JR

更新日期：1988-06-01 00:00:00

abstract：:The pH dependence of the activity of Cu,Zn superoxide dismutases from bovine erythrocytes and shark liver was studied by pulse radiolysis in both the native enzymes and those chemically modified at lysine side chains. The study was aimed at identifying the residues responsible for the activity decrease at pH > 9, obse...

journal_title：Archives of biochemistry and biophysics

authors： Polticelli F,O'Neill P,Costanzo S,Lania A,Rotilio G,Desideri A

更新日期：1995-08-01 00:00:00

abstract：:Glucose-6-phosphatase is a multicomponent system located in the endoplasmic reticulum, involving both a catalytic subunit (G6PC) and several substrate and product carriers. The glucose-6-phosphate carrier is called G6PT1. Using light scattering, we determined K(D) values for phosphate and glucose transport in rat live...

journal_title：Archives of biochemistry and biophysics

authors： Clottes E,Middleditch C,Burchell A

更新日期：2002-12-01 00:00:00

abstract：:No major structural alteration of alkaline phosphatase can be observed in the early stages of enzyme oxidative inactivation by the ascorbate model system. Fluorescence changes of protein-bound 8-anilino-1-naphthalenesulfonic acid suggest, however, that localized modifications take place. Oxidized alkaline phosphatase ...

journal_title：Archives of biochemistry and biophysics

authors： Mordente A,Martorana GE,Miggiano GA,Meucci E,Santini SA,Castelli A

更新日期：1988-08-01 00:00:00

abstract：:Eleven hybridoma clones which secrete monoclonal antibodies against purified rat liver alcohol dehydrogenase (EC 1.1.1.1) were isolated. Antibodies (R-1-R-11) were identified by their ability to bind to immobilized pure alcohol dehydrogenase in an enzyme-linked immunoadsorbent assay, in which antibody R-9 showed the h...

journal_title：Archives of biochemistry and biophysics

authors： Lad PJ,Schenk DB,Leffert HL

更新日期：1984-12-01 00:00:00

abstract：:2,2'-Anhydro-3'-deoxy-5-ethyluridine, a new pyrimidine nucleoside analog, has been examined in terms of its binding potency to uridine phosphorylase, and its conformation in solution (NMR) was studied. 2,2'-Anhydro-3'-deoxy-5-ethyluridine has a Ki value of 3.4 microM for uridine phosphorylase from rat intestinal mucos...

journal_title：Archives of biochemistry and biophysics

authors： Veres Z,Neszmélyi A,Szabolcs A,Kiss AI,Dénes G

更新日期：1991-04-01 00:00:00

abstract：:Purified NAD-malic enzyme from Ascaris suum is rapidly inactivated by the arginine reagent, 2,3-butanedione, and this inactivation is facilitated by 30 mM borate. Determination of the inactivation rate as a function of butanedione concentration suggests a second-order process overall, which is first order in butanedio...

journal_title：Archives of biochemistry and biophysics

authors： Rao GS,Kong CT,Benjamin RC,Harris BG,Cook PF

更新日期：1987-05-15 00:00:00

abstract：:The reaction mechanism of a 1,4-benzoquinone reductase from the wood-rotting basidiomycete Phanerochaete chrysosporium was investigated. The native, oxidized, FMN-containing enzyme was reduced quantitatively by NADH and the resulting reduced enzyme was reoxidized in the presence of one equivalent of 2,6-di-methoxy-1,4...

journal_title：Archives of biochemistry and biophysics

authors： Brock BJ,Gold MH

更新日期：1996-07-01 00:00:00

abstract：:The mechanism by which dietary cholesterol regulates expression of the hepatic low-density lipoprotein (LDL) receptor was investigated. In a previous study (Arch. Biochem. Biophys. 325, 242-248, 1996), we demonstrated that dietary cholesterol reduces the rate of LDL receptor protein degradation without affecting stead...

journal_title：Archives of biochemistry and biophysics

authors： Lopez D,Ness GC

更新日期：1997-08-01 00:00:00

abstract：:Mycobactin acylation plays a crucial role in the ability of Mycobacterium tuberculosis to acquire intracellular iron during infection. M. tuberculosis Rv1347c, the lysine N(epsilon)-acyltransferase responsible for mycobactin acylation, represents a valid target for the development of novel anti-tubercular agents. Here...

journal_title：Archives of biochemistry and biophysics

authors： Frankel BA,Blanchard JS

更新日期：2008-09-15 00:00:00

abstract：:Bovine liver glutamate dehydrogenase reacts covalently with 2-(4-bromo-2,3-dioxobutylthio)adenosine 5'-monophosphate (2-BDB-TAMP) with incorporation of 1 mol reagent/mol enzyme subunit and loss of one of the two ADP sites of native enzyme [S. P. Batra and R. F. Colman, J. Biol. Chem. 261, 15565-15571 (1986)]. Incorpor...

journal_title：Archives of biochemistry and biophysics

authors： Batra SP,Lark RH,Colman RF

更新日期：1989-04-01 00:00:00