Inhibition of rabbit beta-globin synthesis by complementary oligonucleotides: identification of mRNA sites sensitive to inhibition.

abstract：:The effect of phenothiazines (trifluoperazine, chlorpromazine, methochlorpromazine, and imipramine) on Ca2+ fluxes in light and heavy sarcoplasmic reticulum (SR) isolated from rabbit fast-twitch skeletal muscle was investigated. These drugs inhibited Ca2+ loading and (Ca2+,Mg2+)-ATPase activity, but had no effect on u...

journal_title：Archives of biochemistry and biophysics

authors： Volpe P,Costello B,Chu A,Fleischer S

更新日期：1984-08-15 00:00:00

abstract：:The secretion of newly synthesized apolipoprotein E (apo E) by human monocyte-derived macrophages (HMD macrophages) was measured in the medium of cells which had been incubated for 24 h with or without either native or acetylated low-density lipoproteins (LDL or AcLDL, respectively), and subsequently with [35S]methion...

journal_title：Archives of biochemistry and biophysics

authors： Kayden HJ,Maschio F,Traber MG

更新日期：1985-06-01 00:00:00

abstract：:A technique is presented for the preparation of three major proteoglycans from 14-day embryonic chicken retinas following their culture overnight with [35S]sulfate and either [3H]glucosamine or [3H]serine. Homogenization of the tissue in saline permitted extraction of heterogeneous soluble proteoglycans separately fro...

journal_title：Archives of biochemistry and biophysics

authors： Morris JE

更新日期：1984-11-15 00:00:00

abstract：:9-Hydrazinoacridine irreversibly labeled pig plasma amine oxidase by covalent attachment to the active carbonyl cofactor. The visible absorption spectrum of the modified protein displays new absorption bands at 495 and 525 nm. Its emission spectrum exhibited maxima at 415 and 440 nm. In addition, both absorption and e...

journal_title：Archives of biochemistry and biophysics

authors： Lamkin MS,Williams TJ,Falk MC

更新日期：1988-02-15 00:00:00

abstract：:The sarcoplasmic reticulum of rabbit skeletal muscle contains a small "proteolipid," i.e., a protein which is soluble in acidic CHCl3/CH3OH. We propose the name sarcolipin for this small protein, to signify its lipid-like solubility and association with the sarcoplasmic reticulum. We have determined the following amin...

journal_title：Archives of biochemistry and biophysics

authors： Wawrzynow A,Theibert JL,Murphy C,Jona I,Martonosi A,Collins JH

更新日期：1992-11-01 00:00:00

abstract：:Investigations into the enzymes responsible for the reductive activation of antineoplastic agents are of particular interest with regard to the use of these agents in the treatment of solid tumors. Xanthine oxidase (EC 1.1.3.22; XO) and xanthine dehydrogenase (EC 1. 1.1.204; XDH) are two enzymes capable of the reducti...

journal_title：Archives of biochemistry and biophysics

authors： Yee SB,Pritsos CA

更新日期：1997-11-15 00:00:00

abstract：:The amino acid L-lysine is synthesized in Saccharomyces cerevisiae via the α-aminoadipate pathway. An as yet unidentified PLP-containing aminotransferase is thought to catalyze the formation of α-aminoadipate from α-ketoadipate in the L-lysine biosynthetic pathway that could be the yeast Aro8 gene product. A screen of...

journal_title：Archives of biochemistry and biophysics

authors： Karsten WE,Reyes ZL,Bobyk KD,Cook PF,Chooback L

更新日期：2011-12-01 00:00:00

abstract：:The major phenobarbital-inducible form of cytochrome P-450 (cytochrome P-450 PB) was purified to homogeneity from rat liver microsomes and rabbit antibodies prepared against the purified enzyme. Using these antibodies, an enzyme-linked immunosorbent assay (ELISA) was developed for the detection of cytochrome P-450 PB ...

journal_title：Archives of biochemistry and biophysics

authors： Seidel SL,Shawver LK,Shires TK

更新日期：1984-03-01 00:00:00

abstract：:Carbohydrate response element binding protein (ChREBP) is a transcription factor that activates liver glycolytic and lipogenetic enzyme genes in response to high carbohydrate diet. Here we report the transcriptional regulatory mechanisms for the rat ChREBP gene. Firstly, we determined the transcription initiation site...

journal_title：Archives of biochemistry and biophysics

authors： Satoh S,Masatoshi S,Shou Z,Yamamoto T,Ishigure T,Semii A,Yamada K,Noguchi T

更新日期：2007-05-01 00:00:00

abstract：:The vesicle-to-micelle structural transitions that occurred in the interaction of sodium dodecyl sulfate with phosphatidylcholine vesicles were studied at the equilibrium by means of dynamic light scattering (at different scattering angles) and freeze-fracture electron microscopy techniques. The incorporation of surfa...

journal_title：Archives of biochemistry and biophysics

authors： López O,Cócera M,Wehrli E,Parra JL,de la Maza A

更新日期：1999-07-15 00:00:00

abstract：:The short term response of the L-6 cell line of rat skeletal myoblasts to elevated extracellular iron concentrations was studied. It was found in all cases that iron as the nitrilotriacetate (NTA) chelate was effective at donating iron to the cells and at stimulating ferritin synthesis. After 48 h in 50 microM ferric ...

journal_title：Archives of biochemistry and biophysics

authors： Rittling SR,Woodworth RC

更新日期：1983-08-01 00:00:00

abstract：:Leukotoxin (ltx) and isoleukotoxin (iltx) methyl esters, are metabolites of methyl linoleic acid, an essential fatty acid. They have been associated with acute respiratory distress syndrome. The observed toxicity of ltx and iltx is, in fact, due to the metabolism of the epoxides to their corresponding diols by soluble...

journal_title：Archives of biochemistry and biophysics

authors： Greene JF,Williamson KC,Newman JW,Morisseau C,Hammock BD

更新日期：2000-04-15 00:00:00

abstract：:The rate constant for quenching, kq, of the phosphorescence of 6-bromo-2-naphthyl sulfate (BNS) by cytochromes c, cytochrome c peroxidase, catalase, and myoglobin has been measured as a function of temperature and solvent viscosity. In aqueous solution at pH 7.0 for cytochromes c and myoglobin the value of kq is nearl...

journal_title：Archives of biochemistry and biophysics

authors： Beckham S,Cook MP,Karki L,Luchsinger MM,Whitlock VR,Wu Y,Zhang Q,Schuh MD

更新日期：1994-05-01 00:00:00

abstract：:Diesters of 3-hydroxy C8, C10, and C12 acids, the female mallard duck pheromones, were found as the major products of the uropygial glands only during the breeding season. The 3-hydroxy acids were identified by mass spectrometry of the trimethylsilyl ethers of the methyl esters and of the diols derived from LiAlH4 red...

journal_title：Archives of biochemistry and biophysics

authors： Kolattukudy PE,Rogers L

更新日期：1987-01-01 00:00:00

abstract：:We have previously shown that melittin, a bee venom peptide, potently inhibited the catalytic and transport functions of rabbit gastric (H+ + K+)ATPase. A radioactive photoaffinity analog of melittin, ([125I]azidosalicylyl melittin), labeled the (H+ + K+)ATPase. These results suggested that melittin exerted inhibitory...

journal_title：Archives of biochemistry and biophysics

authors： Cuppoletti J

更新日期：1990-05-01 00:00:00

abstract：:Hyaluronan controls keratinocyte proliferation and regeneration. We examined effect of UV on the expression of hyaluronan synthases (HASs) and hyaluronidases in cultured normal human newborn foreskin epidermal keratinocytes, NHEK(F). HAS3 mRNA was expressed predominantly and HAS2 mRNA expressed in lesser amounts and b...

journal_title：Archives of biochemistry and biophysics

authors： Kakizaki I,Itano N,Kimata K,Hanada K,Kon A,Yamaguchi M,Takahashi T,Takagaki K

更新日期：2008-03-01 00:00:00

abstract：:Low-temperature plasma is useful for the care of wounded skin. It accelerates wound healing. However, the mechanism of this effect has not been fully elucidated yet. Galectin-1 is reported to accelerate wound healing via the Smad signaling pathway. In the present study to clarify whether or not galectins were expresse...

journal_title：Archives of biochemistry and biophysics

authors： Akimoto Y,Ikehara S,Yamaguchi T,Kim J,Kawakami H,Shimizu N,Hori M,Sakakita H,Ikehara Y

更新日期：2016-09-01 00:00:00

abstract：:Forskolin, a diterpene that exerts several pharmacological effects, activates adenylate cyclase in brain and in some other mammalian tissues. Properties of forskolin activation of adenylate cyclase from central nervous system of the dipterous Ceratitis capitata are described. The interaction of forskolin with the inse...

journal_title：Archives of biochemistry and biophysics

authors： Guillen A,Haro A,Municio AM

更新日期：1987-04-01 00:00:00

abstract：:We have studied the effectiveness of polyprenyl-P-mannose and polyprenol-P-glucose as donor substrates for the dolichyl-P-mannose:Man5(GlcNAc)2-PP-dolichol mannosyltransferase and the dolichyl-P-glucose:Man9(GlcNAc)2-PP-dolichol glucosyltransferase, respectively. The polyprenol moiety differs from dolichol only in the...

journal_title：Archives of biochemistry and biophysics

authors： D'Souza-Schorey C,McLachlan KR,Krag SS,Elbein AD

更新日期：1994-02-01 00:00:00

abstract：:We previously reported that rat testis and porcine testis contain a physicochemically indistinguishable Mr 32,000 calmodulin-binding protein, which we named "calspermin." In this study, antiserum against calspermin from porcine testis was raised in a rabbit, and a radioimmunoassay was developed. The antiserum reacted ...

journal_title：Archives of biochemistry and biophysics

authors： Ono T,Koide Y,Arai Y,Yamashita K

更新日期：1987-05-15 00:00:00

abstract：:The alcohol-inducible P450 2E subfamily in the rabbit has two known members that differ in only 16 amino acid residues scattered throughout the polypeptide chain. P450 2E1 has been thoroughly characterized, and is known to have diverse inducers and substrates. Little is known, however, about the properties of P450 2E2...

journal_title：Archives of biochemistry and biophysics

authors： Ding XX,Pernecky SJ,Coon MJ

更新日期：1991-12-01 00:00:00

abstract：:The Ah receptor from rat liver has been purified, using a specific oligonucleotide affinity column, in order to characterize the components of the receptor and to investigate features that modulate its DNA-binding activity. The purified DNA-binding form of rat Ah receptor contains three major components, with estimate...

journal_title：Archives of biochemistry and biophysics

authors： Ireland RC,Li SY,Dougherty JJ

更新日期：1995-06-01 00:00:00

abstract：:Hepatic flavin-containing monooxygenases catalyze NADPH-dependent oxygenation of a wide variety of drugs that possess a nucleophilic heteroatom. Two forms of these enzymes (form 1 and 2) have been isolated from rabbit liver microsomes and partially characterized (Ozols, J., 1989, Biochem. Biophys. Res. Commun. 163, 49...

journal_title：Archives of biochemistry and biophysics

authors： Ozols J

更新日期：1991-10-01 00:00:00

abstract：:Sulfhydryl-specific reagents were used to study the reactivities and function of the four cysteinyl residues per subunit present in Salmonella typhimurium 5-phosphoribosyl-alpha-1-pyrophosphate (PRPP) synthetase. In the presence of high concentrations of denaturants all four cysteinyl residues reacted with sulfhydryl-...

journal_title：Archives of biochemistry and biophysics

authors： Harlow KW,Switzer RL

更新日期：1990-02-01 00:00:00

abstract：:Tumor necrosis factor-alpha (TNF-alpha) binds to TNF-alpha receptors (TNFR) to produce a hexameric (TNF-alpha)(3)-(TNFR)(3) structure that stimulates apoptosis. We found by using ELISA that TNF-alpha binds to the glycosylphosphatidylinositol (GPI) anchor glycans of carcinoembryonic antigen, human placental alkaline ph...

journal_title：Archives of biochemistry and biophysics

authors： Fukushima K,Ishiyama C,Yamashita K

更新日期：2004-06-15 00:00:00

abstract：:Abnormal production of matrix metalloproteinases (MMPs) has been observed in a variety of diseases, such as emphysema, atherosclerosis, and cancer metastasis. Destruction of connective tissue ensues and elastin is often a key target. Three of the main elastolytic MMPs are the gelatinases MMP-2 and MMP-9 and the metall...

journal_title：Archives of biochemistry and biophysics

authors： Jensen SA,Andersen P,Vrhovski B,Weiss AS

更新日期：2003-01-15 00:00:00

abstract：:Procedures for the purification of bovine muscle carbonic anhydrase (isoenzyme III) are described. The purified enzyme has a molecular weight near 29,000 and contains one Zn2+ ion per molecule. The sedimentation coefficient, s(0)20,w, is 2.8 X 10(-13) s, the isoelectric pH is 8.5, and A280(0.1%) = 2.07 cm-1. The CO2 h...

journal_title：Archives of biochemistry and biophysics

authors： Engberg P,Millqvist E,Pohl G,Lindskog S

更新日期：1985-09-01 00:00:00

abstract：:Synechococcus sp. strain PCC 7002 is a robust, genetically tractable cyanobacterium that produces six different xanthophyll carotenoids (zeaxanthin, cryptoxanthin, myxoxanthophyll (myxol-2'-fucoside), echinenone, 3'-hydroxyechinenone, and synechoxanthin) and tolerates many environmental stresses, including high light ...

journal_title：Archives of biochemistry and biophysics

authors： Zhu Y,Graham JE,Ludwig M,Xiong W,Alvey RM,Shen G,Bryant DA

更新日期：2010-12-01 00:00:00

abstract：:Treatment of human pulmonary artery smooth muscle cells (HPASMCs) with the thromboxane A2 receptor antagonist, SQ29548 inhibited U46619 stimulation of phospholipase D (PLD) and NADPH oxidase activities in the cell membrane. Pretreatment with apocynin inhibited U46619 induced increase in NADPH oxidase activity. The cel...

journal_title：Archives of biochemistry and biophysics

authors： Chakraborti S,Sarkar J,Chowdhury A,Chakraborti T

更新日期：2017-11-01 00:00:00

abstract：:An amino acid-sensitive form of chorismate mutase (CM) has been purified over 1000-fold from disks excised from tubers of Solanum tuberosum L. cv White Rose. Purification was accomplished by chromatography on Matrix Blue A followed by affinity chromatography with tryptophan as ligand. CM assays performed in the absenc...

journal_title：Archives of biochemistry and biophysics

authors： Kuroki GW,Conn EE

更新日期：1988-02-01 00:00:00