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Mechanism of the aromatic aminotransferase encoded by the Aro8 gene from Saccharomyces cerevisiae.

Abstract：

:The amino acid L-lysine is synthesized in Saccharomyces cerevisiae via the α-aminoadipate pathway. An as yet unidentified PLP-containing aminotransferase is thought to catalyze the formation of α-aminoadipate from α-ketoadipate in the L-lysine biosynthetic pathway that could be the yeast Aro8 gene product. A screen of several different amino acids and keto-acids showed that the enzyme uses L-tyrosine, L-phenylalanine, α-ketoadipate, and L-α-aminoadipate as substrates. The UV-visible spectrum of the aminotransferase exhibits maxima at 280 and 343 nm at pH 7.5. As the pH is decreased the peak at 343 nm (the unprotonated internal aldimine) disappears and two new peaks at 328 and 400 nm are observed representing the enolimine and ketoenamine tautomers of the protonated aldimine, respectively. Addition, at pH 7.1, of α-ketoadipate to free enzyme leads to disappearance of the absorbance at 343 nm and appearance of peaks at 328 and 424 nm. The V/E(t) and V/K(α-ketoadipate)E(t) pH profiles are pH independent from pH 6.5 to 9.6, while the V/K(L-tyrosine) pH-rate profile decreases below a single pK(a) of 7.0 ± 0.1. Data suggest the active enzyme form is with the internal aldimine unprotonated. We conclude the enzyme should be categorized as a α-aminoadipate aminotransferase.

journal_name

Arch Biochem Biophys

journal_title

Archives of biochemistry and biophysics

authors

Karsten WE,Reyes ZL,Bobyk KD,Cook PF,Chooback L

doi

10.1016/j.abb.2011.09.008

subject

Has Abstract

pub_date

2011-12-01 00:00:00

pages

67-74

issue

1

eissn

0003-9861

issn

1096-0384

pii

S0003-9861(11)00319-5

journal_volume

516

pub_type

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