Alternative diaspirins for modification of hemoglobin and sickle hemoglobin.

abstract：:Ca2+ binding and internalization in sarcoplasmic reticulum ATPase can be investigated by the use of La3+ as a Ca2+ analog. Displacement kinetics of Ca2+ bound by La3+ in native vesicles is a slow biphasic process (k1 = 0.55 s-1 and k2 = 0.05 s-1) that is consistent with the existence of two Ca2+ binding populations wh...

journal_title：Archives of biochemistry and biophysics

authors： Fernandez-Belda F

更新日期：1988-12-01 00:00:00

abstract：:Dethiolation of proteins (reduction of protein mixed disulfides) by NADPH-dependent and glutathione (GSH)-dependent enzymes, and by nonenzymatic reaction with GSH, was studied by electrofocusing methodology with glycogen phosphorylase b and creatine kinase as substrates. Phosphorylase b was not rapidly dethiolated by ...

journal_title：Archives of biochemistry and biophysics

authors： Park EM,Thomas JA

更新日期：1989-10-01 00:00:00

abstract：:Changes in the levels of various molecular species of N-acylethanolamine in CdCl2-administered rat testis were examined. We found that the levels of various N-acylethanolamines including anandamide (N-arachidonoylethanolamine), an endogenous cannabinoid receptor ligand, were dramatically increased in CdCl2-admin-ister...

journal_title：Archives of biochemistry and biophysics

authors： Kondo S,Sugiura T,Kodaka T,Kudo N,Waku K,Tokumura A

更新日期：1998-06-15 00:00:00

abstract：:Triosephosphate isomerase from rabbit has 5 Cys and 2 Met, while triosephosphate isomerase from yeast has 2 Cys (present in the rabbit enzyme in equivalent positions) and no Met. Since chloramine-T oxidizes Cys and Met, we determined the effect it has on the activity and structure of both enzymes. The activity of trio...

journal_title：Archives of biochemistry and biophysics

authors： Zubillaga RA,Pérez-Montfort R,Gómez-Puyou A

更新日期：1994-09-01 00:00:00

abstract：:Renally excreted 8-oxo-7,8-dihydro-2(')-deoxyguanosine (oxo(8)dG) is a potential marker of oxidative DNA damage by reactive oxygen species. Whole-body degradation rates of t- and rRNA are potential indicators of the resting metabolic rate (RMR). Excretion rates of oxo(8)dG and degradation rates of t- and rRNA were det...

journal_title：Archives of biochemistry and biophysics

authors： Topp H,Armbrust S,Lengger C,Schöch G,Davies J,Stichler W,Manz F,Fusch C

更新日期：2002-06-01 00:00:00

abstract：:IGFBP-3 interacts with the retinoid X receptor-alpha (RXRalpha) and retinoic acid receptor-alpha (RARalpha) and thereby interferes with the formation of RXR:RAR heterodimers. Here we identify the domains in RXRalpha and IGFBP-3 that participate in this interaction. When different regions of RXRalpha were expressed ind...

journal_title：Archives of biochemistry and biophysics

authors： Schedlich LJ,Graham LD,O'Han MK,Muthukaruppan A,Yan X,Firth SM,Baxter RC

更新日期：2007-09-15 00:00:00

abstract：:Tripeptidyl-peptidase II (TPP II) is a cytosolic peptidase that has been implicated in fat formation and cancer, apparently independent of the enzymatic activity. In search for alternative functional regions, conserved motifs were identified and eleven signatures were constructed. Seven of the signatures covered previ...

journal_title：Archives of biochemistry and biophysics

authors： Eriksson S,Gutiérrez OA,Bjerling P,Tomkinson B

更新日期：2009-04-01 00:00:00

abstract：:This paper describes a theory for the mechanism of three-state transition of proteins which is often observed in aqueous organic cosolvent systems, i.e., from the native, via intermediate to helical forms. The first transition, accompanied by changes in the tertiary and/or secondary structures, was explained by larger...

journal_title：Archives of biochemistry and biophysics

authors： Arakawa T,Goddette D

更新日期：1985-07-01 00:00:00

abstract：BACKGROUND:The main functions of the skin are to protect against environmental insults and prevent water loss, which are performed by the complex lipid- and protein matrix present in the outermost layers of the epithelium. The lipidome of these outer layers is mainly composed of ceramides, fatty acids, and cholesterol,...

journal_title：Archives of biochemistry and biophysics

authors： Muresan XM,Narzt MS,Woodby B,Ferrara F,Gruber F,Valacchi G

更新日期：2019-05-15 00:00:00

abstract：:Sodium pump was the first ion pump discovered. A member of the family of active transporters that catalyze adenosine 5'-triphosphate hydrolysis by forming a phosphorylated enzyme intermediate, sodium pump couples the energy released to unequal countertransport of sodium and potassium ions. The ion gradient generated b...

journal_title：Archives of biochemistry and biophysics

authors： Faller LD

更新日期：2008-08-01 00:00:00

abstract：:FHRE-Luc is a promoter reporter construct that is widely used to assess the activity of FoxO (forkhead box, class O) transcription factors. We here demonstrate that this promoter construct responds to exposure of HepG2 human hepatoma cells to known agonists of the aryl hydrocarbon receptor (AhR), 3-methylcholanthrene,...

journal_title：Archives of biochemistry and biophysics

authors： Eckers A,Sauerbier E,Anwar-Mohamed A,Hamann I,Esser C,Schroeder P,El-Kadi AO,Klotz LO

更新日期：2011-12-15 00:00:00

abstract：:The antioxidant, butylated hydroxytoluene (BHT), causes lung toxicity in mice followed by regenerative repair, and can also modulate the development of carcinogen-induced lung adenomas. We are investigating changes in pulmonary biochemistry following BHT treatment in order to understand the mechanisms of BHT-induced p...

journal_title：Archives of biochemistry and biophysics

authors： Blumenthal EJ,Malkinson AM

更新日期：1987-07-01 00:00:00

abstract：:Four subunits of beta-conglycinin were purified from soybean cultivar CX 635-1-1-1, and were designated alpha, alpha', beta, and beta' in accordance with nomenclature proposed by Thanh and Shibasaki [(1977) Biochim. Biophys. Acta 490, 370-384]. Of these subunits, beta' has not previously been reported or characterized...

journal_title：Archives of biochemistry and biophysics

authors： Coates JB,Medeiros JS,Thanh VH,Nielsen NC

更新日期：1985-11-15 00:00:00

abstract：:Conversion of geranyl pyrophosphate to cyclic monoterpenes is considered to involve the preliminary isomerization of this acyclic precursor to enzyme-bound linalyl pyrophosphate and the cyclization of this tertiary intermediate. 2-Fluorogeranyl pyrophosphate and 2-fluorolinalyl pyrophosphate are effective competitive ...

journal_title：Archives of biochemistry and biophysics

authors： Croteau R

更新日期：1986-12-01 00:00:00

abstract：:Free radical-mediated reperfusion injury has been established as an important mechanism leading to post-ischemic reperfusion myocardial damage. The present study was undertaken to determine the protective role of vitamin E, a membrane-bound free-radical scavenger, on ischemia-reperfusion myocardial injury. After 4 mon...

journal_title：Archives of biochemistry and biophysics

authors： Pyke DD,Chan AC

更新日期：1990-03-01 00:00:00

abstract：:Pasteurella multocida is a pathogen of veterinary and medical importance. Here, we report the 1.85Å resolution crystal structure of the class C acid phosphatase from this organism (denoted rPmCCAP). The structure shows that rPmCCAP exhibits the same haloacid dehalogenase fold and dimeric assembly as the class C enzyme...

journal_title：Archives of biochemistry and biophysics

authors： Singh H,Malinski TJ,Reilly TJ,Henzl MT,Tanner JJ

更新日期：2011-05-01 00:00:00

abstract：:Previous studies have indicated that activation of a normally unexpressed beta 1----3-N-acetylglucosaminyltransferase is responsible for the accumulation of a wide diversity of both type 1 and 2 lacto-series antigens in human colonic adenocarcinomas. A beta 1----3-N-acetylglucosaminyltransferase has been solubilized f...

journal_title：Archives of biochemistry and biophysics

authors： Holmes EH

更新日期：1988-01-01 00:00:00

abstract：:We have previously identified and characterized GSHPx-GI, which is a cellular selenium-dependent glutathione peroxidase (GSHPx) distinct from the classic GSHPx-1 and phospholipid hydroperoxide glutathione peroxidase (PHGPX). We have determined the level of GSHPx-GI mRNA expression in the rat gastrointestinal tract fro...

journal_title：Archives of biochemistry and biophysics

authors： Chu FF,Esworthy RS

更新日期：1995-11-10 00:00:00

abstract：:ATP-activated P2Y receptors play an important role in renal sodium excretion. The aim of this study was to evaluate the modulation of ATPase-driven sodium reabsorption in the proximal tubule by ATP or adenosine (Ado). LLC-PK1 cells, a model of porcine proximal tubule cells, were used. ATP (10(-6)M) or Ado (10(-6)M) sp...

journal_title：Archives of biochemistry and biophysics

authors： Wengert M,Ribeiro MC,Abreu TP,Coutinho-Silva R,Leão-Ferreira LR,Pinheiro AA,Caruso-Neves C

更新日期：2013-07-15 00:00:00

abstract：:Incubation of rat liver nuclear envelopes with [gamma-32P]ATP resulted in the synthesis of phosphatidylinositol-[4-32P]phosphate (PIP). Degradation of endogenously labeled PIP was observed upon the dilution of the labeled ATP with an excess of unlabeled ATP. This degradation was most rapid in the presence of EDTA, and...

journal_title：Archives of biochemistry and biophysics

authors： Smith CD,Wells WW

更新日期：1984-12-01 00:00:00

abstract：:A series of novel peptidyl-alpha-ketoamide compounds were evaluated as inhibitors of the deltaNS3-NS4A serine protease from the hepatitis C virus. These peptidyl-alpha-ketoamide inhibitors with Ki values ranging from 0.17 nM to 5.6 microM exhibited slow-binding inhibition. Kinetic studies established one-step kinetic ...

journal_title：Archives of biochemistry and biophysics

authors： Liu Y,Stoll VS,Richardson PL,Saldivar A,Klaus JL,Molla A,Kohlbrenner W,Kati WM

更新日期：2004-01-15 00:00:00

abstract：:The 3-dimensional structure determination of rat phenylalanine hydroxylase (PAH) has identified potentially important amino acids lining the active site cleft with the majority of these having hydrophobic side-chains including several with aromatic side chains. Here we have analyzed the effect on rat PAH enzyme kineti...

journal_title：Archives of biochemistry and biophysics

authors： Jennings IG,Cotton RG,Kobe B

更新日期：2000-12-15 00:00:00

abstract：:Because of the severe limitations on growing large quantities of Drosophila affinidisjuncta in the laboratory, direct purification of alcohol dehydrogenase (ADH) from this species has proven impossible. As an alternative source of this enzyme, a cDNA encoding functional ADH was isolated from a newly constructed cDNA l...

journal_title：Archives of biochemistry and biophysics

authors： Green MM,Fang XM,Churchill P,Brennan MD

更新日期：1989-09-01 00:00:00

abstract：:The zaragozic acids are potent inhibitors of squalene synthase. In vivo studies in mice confirmed our earlier observations that inhibition of squalene synthase by zaragozic acid A was accompanied by an increase in the incorporation of label from [3H]mevalonate into farnesyl-diphosphate (FPP)-derived isoprenoic acids (...

journal_title：Archives of biochemistry and biophysics

authors： Vaidya S,Bostedor R,Kurtz MM,Bergstrom JD,Bansal VS

更新日期：1998-07-01 00:00:00

abstract：:Human hemoglobin (Hb) Coimbra (βAsp99Glu) is one of the seven βAsp99 Hb variants described to date. All βAsp99 substitutions result in increased affinity for O2 and decreased heme-heme cooperativity and their carriers are clinically characterized by erythrocytocis, caused by tissue hypoxia. Since βAsp99 plays an impor...

journal_title：Archives of biochemistry and biophysics

authors： Jorge SE,Bringas M,Petruk AA,Arrar M,Marti MA,Skaf MS,Costa FF,Capece L,Sonati MF,Estrin D

更新日期：2018-01-01 00:00:00

abstract：:Phosphatidylserine (PS) is predominantly confined to the inner leaflet of plasma membrane in cells, but it is externalized on the cell surface during apoptosis. This externalized PS is required for effective phagocytosis of apoptotic cells by macrophages. Because PS trans-bilayer asymmetry is not absolute in different...

journal_title：Archives of biochemistry and biophysics

authors： Borisenko GG,Matsura T,Liu SX,Tyurin VA,Jianfei J,Serinkan FB,Kagan VE

更新日期：2003-05-01 00:00:00

abstract：:Atrial muscles of the heart are known to produce polypeptide hormones called atrial natriuretic factors (ANF) which have potent diuretic and hypotensive action. These hormones are synthesized as a larger protein precursor called pro atrial natriuretic factor or proANF which contains the biologically active ANF sequenc...

journal_title：Archives of biochemistry and biophysics

authors： Gierse JK,Olins PO,Devine CS,Marlay JD,Obukowicz MG,Mortensen LH,McMahon EG,Blaine EH,Seetharam R

更新日期：1989-06-01 00:00:00

abstract：:A fraction containing a protein-glycogen complex was isolated from rat skeletal muscle in order to study the effect of hypothyroidism on phosphorylase activation in this structural and functional unit of the glycogenolytic process. The total activities of phosphorylase and phosphorylase phosphatase in euthyroids and h...

journal_title：Archives of biochemistry and biophysics

authors： Leijendekker WJ,Edauw P,van Hardeveld C,Simonides WS

更新日期：1989-10-01 00:00:00

abstract：:There is dose-dependent Cd(2+)-evoked swelling of isolated rat liver mitochondria energized by complex I, II, or IV respiratory substrates in sucrose medium in the absence of added Ca(2+) and P(i), which is prevented by Sr(2+). Permeability transition effectors (ADP, CsA, EGTA, RR, DTT, ATR, P(i), and Ca(2+)) affect i...

journal_title：Archives of biochemistry and biophysics

authors： Belyaeva EA,Glazunov VV,Korotkov SM

更新日期：2002-09-15 00:00:00

abstract：:In trout and mammals, the major extrahepatic expression site for CYP3A forms is in the intestine. A cDNA encoding a new CYP3A subfamily member was isolated from rainbow trout intestinal ceca by reverse transcriptase-polymerase chain reaction (RT-PCR), followed by rapid amplification of cDNA ends (RACE)-PCR. In a set o...

journal_title：Archives of biochemistry and biophysics

authors： Lee SJ,Buhler DR

更新日期：2003-04-01 00:00:00