Abstract:
:Atrial muscles of the heart are known to produce polypeptide hormones called atrial natriuretic factors (ANF) which have potent diuretic and hypotensive action. These hormones are synthesized as a larger protein precursor called pro atrial natriuretic factor or proANF which contains the biologically active ANF sequences at its C-terminus. Rat proANF (representing amino acids -1 to 128 of the coding sequence) was expressed in a soluble form in Escherichia coli. A simple purification procedure was developed which consists of boiling E. coli cell extracts in 1 M acetic acid and subjecting the supernatant to reversed-phase HPLC. The effect of intravenous administration of the purified recombinant proANF on mean arterial blood pressure was examined. The displacement dose-response curves obtained demonstrated that proANF exhibits similar, albeit less potent, physiological activity than ANF.
journal_name
Arch Biochem Biophysjournal_title
Archives of biochemistry and biophysicsauthors
Gierse JK,Olins PO,Devine CS,Marlay JD,Obukowicz MG,Mortensen LH,McMahon EG,Blaine EH,Seetharam Rdoi
10.1016/0003-9861(89)90294-4subject
Has Abstractpub_date
1989-06-01 00:00:00pages
441-6issue
2eissn
0003-9861issn
1096-0384pii
0003-9861(89)90294-4journal_volume
271pub_type
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