Evidence for requirement of NADPH-cytochrome P450 oxidoreductase in the microsomal NADPH-sterol Delta7-reductase system.

abstract：:While the general catalytic mechanism of the widespread serine hydrolase superfamily has been documented extensively, much less is known about its varied modes of functional modulation within biological systems. Under oxidizing conditions, inhibition of Saccharomyces cerevisiae S-formylglutathione hydrolase (SFGH, hom...

journal_title：Archives of biochemistry and biophysics

authors： Legler PM,Leary DH,Hervey WJ 4th,Millard CB

更新日期：2012-12-01 00:00:00

abstract：:A microsomal retinol dehydrogenase (RoDH) that recognizes holo-cellular retinol binding protein (CRBP) as substrate is inhibited by phenylarsine oxide (IC50 = 3 microM) in the presence of 10 mM cysteine. Inhibition was reversible with dithiothreitol, indicating that two cysteine residues in close proximity are essenti...

journal_title：Archives of biochemistry and biophysics

authors： Boerman MH,Napoli JL

更新日期：1995-08-20 00:00:00

abstract：:Accumulation of carboxymethylated proteins (CML-proteins) is taken as a biomarker of glycoxidative stress which is thought to contribute to the age-related impairment in tissue and cell function. To investigate the occurrence and extent of glycoxidative damage with aging in rat kidney, serum and urine, we have prepare...

journal_title：Archives of biochemistry and biophysics

authors： Hamelin M,Borot-Laloi C,Friguet B,Bakala H

更新日期：2003-03-15 00:00:00

abstract：:In order to search for probable conformations of the peptide, the amino acid side chain, and the carbohydrate linkage in glycoproteins, conformational energy surfaces of glycopeptide model compounds were studied by Monte Carlo methods using the Metropolis algorithm. The potential energies were composed of empirical en...

journal_title：Archives of biochemistry and biophysics

authors： Duben AJ,Bush CA

更新日期：1983-08-01 00:00:00

abstract：:Serine is an essential amino acid for the lectin-mediated transformation of human peripheral blood lymphocytes due to the inability of this cell to synthesize sufficient quantities via either the phosphorylated pathway or by reversal of the serine hydroxymethyltransferase reaction to meet the metabolic demands. The le...

journal_title：Archives of biochemistry and biophysics

authors： Rowe PB,Sauer D,Fahey D,Craig G,McCairns E

更新日期：1985-01-01 00:00:00

abstract：:The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that functions as an intracellular mediator in the xenobiotic signaling pathway. Although a number of studies have examined AhR-mediated CYP1A1 induction in detail, recent studies of AhR-null mice have revealed that AhR plays important regu...

journal_title：Archives of biochemistry and biophysics

authors： Kawajiri K,Fujii-Kuriyama Y

更新日期：2007-08-15 00:00:00

abstract：:Oxidative treatments of wood pulp lignin by one-electron-abstracting enzymatic or chemical systems result in modification phenomena which are not fully described in terms of those known from lignin model compound studies. The generation of, e.g., long-lived radicals necessitates nondestructive spectroscopic analysis o...

journal_title：Archives of biochemistry and biophysics

authors： Barsberg S

更新日期：2002-08-01 00:00:00

abstract：:Two recombinant sesquiterpene synthases from grand fir, delta-selinene synthase and gamma-humulene synthase, each produce more than 30 sesquiterpene olefins from the acyclic precursor farnesyl diphosphate. These enzymes contain a pair of DDxxD motifs, on opposite lips of the presumptive active site, which are thought ...

journal_title：Archives of biochemistry and biophysics

authors： Little DB,Croteau RB

更新日期：2002-06-01 00:00:00

abstract：:The relationship between erythroid delta-aminolevulinate (ALA) synthase and hepatic ALA synthase in rat was analyzed immunochemically, using antibodies directed against rat liver ALA synthase and against chicken liver ALA synthase. Rat erythroid ALA synthase showed no cross-reactivity with anti-liver ALA synthase anti...

journal_title：Archives of biochemistry and biophysics

authors： Yamamoto M,Fujita H,Watanabe N,Hayashi N,Kikuchi G

更新日期：1986-02-15 00:00:00

abstract：:The effect of the vinca alkaloid drugs, vincristine, vinblastine, catharanthine, and vindoline, on the aging process of tubulin has been examined. It was found that addition of vincristine or vinblastine accelerated by a factor of 3-3.5 the transformation of tubulin from the 5.8 S alpha-beta-tubulin dimer to paucidisp...

journal_title：Archives of biochemistry and biophysics

authors： Prakash V,Timasheff SN

更新日期：1992-05-15 00:00:00

abstract：:Glycosylasparaginase (EC 3.5.1.26) is a lysosomal amidase which hydrolyzes the bond between asparagine and the sugar moiety in N-linked glycoproteins. Deficiency of the enzyme results in aspartylglycosaminuria (AGU), the most common disorder of glycoprotein degradation. Mature enzyme is formed by two proteolytic cleav...

journal_title：Archives of biochemistry and biophysics

authors： Park H,Vettese-Dadey M,Aronson NN Jr

更新日期：1996-04-01 00:00:00

abstract：:Differentiation of pluripotent and lineage restricted stem cells such as neural stem cells (NSCs) was studied on conducting substrates of various nature without perturbation of the genome with exogenous genetic material or chemical stimuli. Primary mouse adult neural stem cells (NSCs) and P19 pluripotent embryonal (P1...

journal_title：Archives of biochemistry and biophysics

authors： Ostrakhovitch EA,Byers JC,O'Neil KD,Semenikhin OA

更新日期：2012-12-01 00:00:00

abstract：:Diastolic dysfunction is a clinically significant problem for patients with diabetes and often reflects increased ventricular stiffness. Attached cross-bridges contribute to myocardial stiffness and produce short-range forces, but it is not yet known whether these forces are altered in diabetes. In this study, we test...

journal_title：Archives of biochemistry and biophysics

authors： Chung CS,Mitov MI,Callahan LA,Campbell KS

更新日期：2014-06-15 00:00:00

abstract：:In this report we provide data, for the first time, demonstrating the conversion of the heme moiety of certain cytochrome P-450 and P-420 preparations, to biliverdin, catalyzed by heme oxygenase. We have used purified preparations of cytochromes P-450c, P-450b, P-450/P-420c, or P-450/P-420b as substrates in a heme oxy...

journal_title：Archives of biochemistry and biophysics

authors： Kutty RK,Daniel RF,Ryan DE,Levin W,Maines MD

更新日期：1988-02-01 00:00:00

abstract：:The topological arrangement of cytochrome b561 in the bovine adrenal medullary chromaffin granule membrane was investigated by radiolabeling and immunoprecipitation techniques using antibody raised against the purified cytochrome. The first labeling procedure involved a membrane-permeable amino group labeling reagent,...

journal_title：Archives of biochemistry and biophysics

authors： Duong LT,Fleming PJ

更新日期：1984-01-01 00:00:00

abstract：:While prior studies focusing on male mice suggest a role for sterol carrier protein-2/sterol carrier protein-x (SCP-2/SCP-x; DKO) on hepatic phytol metabolism, its role in females is unresolved. This issue was addressed using female and male wild-type (WT) and DKO mice fed a phytoestrogen-free diet without or with 0.5...

journal_title：Archives of biochemistry and biophysics

authors： McIntosh AL,Storey SM,Huang H,Kier AB,Schroeder F

更新日期：2017-12-01 00:00:00

abstract：:In an effort to model the interaction of lipid-based DNA delivery systems with anionic surfaces, such as a cell membrane, we have utilized microelectrophoresis to characterize how electrokinetic measurements can provide information on surface charge and binding characteristics. We have established that cationic lipids...

journal_title：Archives of biochemistry and biophysics

authors： Wong FM,Bally MB,Brooks DE

更新日期：1999-06-01 00:00:00

abstract：:Uncoupling protein 2 (UCP2) was reported to be involved in insulin-glucose homeostasis, based on well established event that inhibition of UCP2 stimulates insulin secretion in pancreatic beta-cells. However, the role of UCP2 on insulin-stimulated glucose uptake in adipose tissue, which is an indispensable process in i...

journal_title：Archives of biochemistry and biophysics

authors： Zhou H,Zhao J,Zhang X

更新日期：2009-06-01 00:00:00

abstract：:Conversion of arachidonic acid into the vicinal diol fatty acid 12R,13S-dihydroxy-5Z,8Z,10E,14Z-eicosatetraenoic acid using an acetone powder of the marine red alga, Gracilariopsis lemaneiformis, occurred via intermediate formation of 12S-hydroperoxy-5Z,8Z,10E,14Z-eicosatetraenoic acid. Incubations of the linoleic aci...

journal_title：Archives of biochemistry and biophysics

authors： Gerwick WH,Moghaddam M,Hamberg M

更新日期：1991-11-01 00:00:00

abstract：:Nitric oxide (.NO) and peroxynitrite (ONOO-) inhibit enzymes that depend on metal cofactors or oxidizable amino acids for activity. Since xanthine oxidase (XO) is a 2(2Fe2S) enzyme having essential sulfhydryl groups linked with Mo-pterin cofactor function, the influence of .NO and ONOO- on purified bovine XO was deter...

journal_title：Archives of biochemistry and biophysics

authors： Houston M,Chumley P,Radi R,Rubbo H,Freeman BA

更新日期：1998-07-01 00:00:00

abstract：:Many of the compounds considered for use in pharmaceutical formulations demonstrate incompatibilities with other components at high enough concentrations, including pairs of polymers, polymers and salts, or even proteins in combination with polymers, salts, or other proteins. Freeze concentration can force solutions i...

journal_title：Archives of biochemistry and biophysics

authors： Heller MC,Carpenter JF,Randolph TW

更新日期：1999-03-15 00:00:00

abstract：:Several 2-halo-acyl-CoA derivatives have been synthesized to examine the interaction of these potential inhibitors of mitochondrial beta-oxidation with the purified medium chain acyl-CoA dehydrogenase from pig kidney. Racemic 2-bromo-, 2-chloro-, and 2-fluoro-octanoyl-CoA thioesters show 6.6, 33, and 3.5% of the activ...

journal_title：Archives of biochemistry and biophysics

authors： Cummings JG,Thorpe C

更新日期：1993-04-01 00:00:00

abstract：:Phosphatidylinositol 3-kinase (PI3-kinase) is known to be a crucial regulator of muscle differentiation. However, its downstream pathway for this function is quite obscure. In this experiment we demonstrated the regulatory mechanism of the differentiation of H9c2 cardiomyoblasts, focusing on PI3-kinase, protein kinase...

journal_title：Archives of biochemistry and biophysics

authors： Kim JM,Yoon MY,Kim J,Kim SS,Kang I,Ha J,Kim SS

更新日期：1999-07-01 00:00:00

abstract：:The effect of phenothiazines (trifluoperazine, chlorpromazine, methochlorpromazine, and imipramine) on Ca2+ fluxes in light and heavy sarcoplasmic reticulum (SR) isolated from rabbit fast-twitch skeletal muscle was investigated. These drugs inhibited Ca2+ loading and (Ca2+,Mg2+)-ATPase activity, but had no effect on u...

journal_title：Archives of biochemistry and biophysics

authors： Volpe P,Costello B,Chu A,Fleischer S

更新日期：1984-08-15 00:00:00

abstract：:Studies of modification of hemoglobin and of sickle hemoglobin by alternative aspirins have been extended to a series of new bis esters with a variety of substituted bridging diacids and to a group of mono esters with polar acyl groups. Rates of hydrolysis of these alternative aspirins have also been examined, and the...

journal_title：Archives of biochemistry and biophysics

authors： Delaney EJ,Massil SE,Shi GY,Klotz IM

更新日期：1984-02-01 00:00:00

abstract：:Using fractionation procedures such as sucrose gradient sedimentation and gel permeation chromatography, a novel cytosolic binding protein for benzo[a]pyrene has been detected in liver of nonmammalian and mammalian species including human. This protein, called 8 S protein, cosediments with the Ah receptor after centri...

journal_title：Archives of biochemistry and biophysics

authors： Lesca P,Peryt B,Soues S,Maurel P,Cravedi JP

更新日期：1993-05-15 00:00:00

abstract：:Signal transducer and activator of transcription 3 (STAT3) is expressed aberrantly in multiple tumors, including gastric cancer (GC). STAT3 overexpression and excessive activation have been confirmed to play vital roles in tumorigenesis. Cucurbitacin B (CuB) is a natural product with potent anti-cancer activities in s...

journal_title：Archives of biochemistry and biophysics

authors： Xu J,Chen Y,Yang R,Zhou T,Ke W,Si Y,Yang S,Zhang T,Liu X,Zhang L,Xiang K,Guo Y,Liu Y

更新日期：2020-05-15 00:00:00

abstract：:We have characterized a Brassica rapa mutant (WR586) that has low levels of polyunsaturated fatty acids (D.L. Auld et al., 1992, Crop Sci. 32, 657-662). The mutant lacked oleoyl-phosphatidylcholine desaturase (ODS) activity when assayed in 6-day-old seedlings. To further characterize the mutant, the leaf fatty acid co...

journal_title：Archives of biochemistry and biophysics

authors： Lee MS,Guerra DJ

更新日期：1994-11-15 00:00:00

abstract：:Nickel (Ni) is widely present in the occupational environment and causes various adverse effects on the human body. Apoptosis induced by Ni2+ may be a key mechanism underlying its toxic effect. In the present study, we investigated the effect of Ni-smelting fumes on cell viability, mitochondrial damage, and apoptosis-...

journal_title：Archives of biochemistry and biophysics

authors： Pan YL,Xin R,Wang SY,Wang Y,Zhang L,Yu CP,Wu YH

更新日期：2018-12-15 00:00:00

abstract：:Abnormal production of matrix metalloproteinases (MMPs) has been observed in a variety of diseases, such as emphysema, atherosclerosis, and cancer metastasis. Destruction of connective tissue ensues and elastin is often a key target. Three of the main elastolytic MMPs are the gelatinases MMP-2 and MMP-9 and the metall...

journal_title：Archives of biochemistry and biophysics

authors： Jensen SA,Andersen P,Vrhovski B,Weiss AS

更新日期：2003-01-15 00:00:00