Chemiluminescent aerobic oxidation of protein adducts with glycolaldehyde catalyzed by horseradish peroxidase.

abstract：:The conversion of the precursor of apolipoprotein A-I (proapoA-I) to apolipoprotein A-I (apoA-I) is known to occur extracellularly by an enzyme that has been shown to be present in plasma. The hepatocarcinoma-derived cell line Hep G2, when grown in culture, secretes proapoA-I. We now show that this cell line also secr...

journal_title：Archives of biochemistry and biophysics

authors： Edelstein C,Kaiser M,Piras G,Scanu AM

更新日期：1988-11-15 00:00:00

abstract：:The absorption spectra of Ni(II) bound to the core histone tetramer, (H3-H4)2, of chicken erythrocytes in 500 mM NaCl + 100 mM phosphate (pH 7.4) were recorded. A charge transfer band was seen at 317 nm, characteristic of a bond between Ni(II) and the sulfur atom of Cys-110 of histone H3. The conditional affinity cons...

journal_title：Archives of biochemistry and biophysics

authors： Bal W,Karantza V,Moudrianakis EN,Kasprzak KS

更新日期：1999-04-15 00:00:00

abstract：:The crystalline beef liver protein of Sumner and Dounce (A. L. Dounce, P. Z. Allen, and G. A. Mourtzikos (1978) Arch. Biochem. Biophys. 188, 251-265) termed FTBL (football) protein because of the shape of its crystals, has been identified as a crystalline leucine aminopeptidase (LAP), on the basis of its high specific...

journal_title：Archives of biochemistry and biophysics

authors： Dounce AL,Allen PZ

更新日期：1987-08-15 00:00:00

abstract：:A gold(III) compound [Au(C^N^C)(IMe)]CF(3)SO(3) (Gold III) has been reported to have anticancer properties as it is able to reduce topoisomerase IB activity in vitro and suppress tumor growth in nude mice model. Here we have investigated the mechanism of inhibition of human topoisomerase IB activity by this compound, ...

journal_title：Archives of biochemistry and biophysics

authors： Castelli S,Vassallo O,Katkar P,Che CM,Sun RW,Desideri A

更新日期：2011-12-15 00:00:00

abstract：:Aryl hydrocarbon hydroxylase activity is most closely associated with cytochrome P-450c in the rat and cytochrome P1-450 in the mouse. The sequence for the orthologous human gene coding for this enzymatic activity has been determined from several sources: cytochrome P-450c isolated from human embryonic DNA [K. Kawajir...

journal_title：Archives of biochemistry and biophysics

authors： Iversen PL,Heiger WJ,Bresnick E,Hines RN

更新日期：1987-07-01 00:00:00

abstract：:We investigated the role of free radicals in hemoglobin (Hb) oxidation and denaturation. To generate free radicals, we used two azocompounds, the hydrophilic 2,2'-azobis(2-amidinopropane hydrochloride and the hydrophobic 2,2'-azobis(2,4-dimethylvaleronitrile) and a drug of the quinone family, phenazine methosulfate. T...

journal_title：Archives of biochemistry and biophysics

authors： Minetti M,Mallozzi C,Scorza G,Scott MD,Kuypers FA,Lubin BH

更新日期：1993-04-01 00:00:00

abstract：:An in vitro expression plasmid (pGRAP) that contained the cDNA coding for the rat mitochondrial aldehyde dehydrogenase precursor was constructed, mRNA was synthesized then translated, and the in vitro synthesized precursor of aldehyde dehydrogenase was used in an in vitro import assay. As expected the 19 amino acid si...

journal_title：Archives of biochemistry and biophysics

authors： Wang TT,Farrés J,Weiner H

更新日期：1989-08-01 00:00:00

abstract：:A new enzyme homologous to phosphotriesterase was identified from the bacterium Geobacillus stearothermophilus (GsP). This enzyme belongs to the amidohydrolase family and possesses the ability to hydrolyze both lactone and organophosphate (OP) compounds, making it a phosphotriesterase-like lactonase (PLL). GsP possess...

journal_title：Archives of biochemistry and biophysics

authors： Hawwa R,Aikens J,Turner RJ,Santarsiero BD,Mesecar AD

更新日期：2009-08-15 00:00:00

abstract：:In the presence of rat liver cytosol and homocysteine, trimethylselenonium ion (TMSe+) underwent time-dependent demethylation to dimethylselenide with the concurrent formation of methionine. Convenient methods were developed for assay of this activity using either radioactive methods based on the gamma emitting isotop...

journal_title：Archives of biochemistry and biophysics

authors： Goeger DE,Ganther HE

更新日期：1993-04-01 00:00:00

abstract：:Histone-DNA templates for bacteriophage T7 RNA polymerase were assembled from a plasmid containing a promoter and a terminator for this polymerase, (H3 x H4)2 tetramers deprived of their tail domains, and H2A x H2B dimers. Histone (H3 x H4)2 tetramers lacking their terminal domains were obtained from trypsin-digested ...

journal_title：Archives of biochemistry and biophysics

authors： Hernández F,López-Alarcón L,Puerta C,Palacián E

更新日期：1998-10-01 00:00:00

abstract：:The 3-dimensional structure determination of rat phenylalanine hydroxylase (PAH) has identified potentially important amino acids lining the active site cleft with the majority of these having hydrophobic side-chains including several with aromatic side chains. Here we have analyzed the effect on rat PAH enzyme kineti...

journal_title：Archives of biochemistry and biophysics

authors： Jennings IG,Cotton RG,Kobe B

更新日期：2000-12-15 00:00:00

abstract：:Vitronectin is a 70-kDa protein that is found in both the extracellular matrix as well as serum. Vitronectin is one of the few proteins that regulates both the complement and the coagulation systems. Heparin is known to bind to vitronectin. Review of the literature reveals apparently conflicting outcomes of the intera...

journal_title：Archives of biochemistry and biophysics

authors： Edens RE,LeBrun LA,Linhardt RJ,Kaul PR,Weiler JM

更新日期：2001-07-15 00:00:00

abstract：:We analyzed the effect of a natural osmolyte, trimethylamine N-oxide (TMAO), on structural properties and conformational stabilities of several proteins under macromolecular crowding conditions by a set of biophysical techniques. We also used the solvent interaction analysis method to look at the peculiarities of the ...

journal_title：Archives of biochemistry and biophysics

authors： Breydo L,Sales AE,Ferreira L,Fedotoff O,Shevelyova MP,Permyakov SE,Kroeck KG,Permyakov EA,Zaslavsky BY,Uversky VN

更新日期：2015-03-15 00:00:00

abstract：:The complete amino acid sequence of Rapana thomasiana hemocyanin functional unit RtH2-e was determined by direct sequencing and matrix-assisted laser desorption ionization mass spectrometry of peptides obtained by cleavage with EndoLysC proteinase, chymotrypsin, and trypsin. The single-polypeptide chain of RtH2-e cons...

journal_title：Archives of biochemistry and biophysics

authors： Stoeva S,Idakieva K,Betzel C,Genov N,Voelter W

更新日期：2002-03-15 00:00:00

abstract：:We isolated two new dihydrooxopyridine cross-links, oxodesmosine (OXD) and isooxodesmosine (IOXD) from the acid hydrolysates of the bovine aortic elastin. OXD and IOXD were identified to have N-substituted 1,2-dihydro-2-oxopyridine and N-substituted 1,4-dihydro-4-oxopyridine skeletons, respectively, with three alpha-a...

journal_title：Archives of biochemistry and biophysics

authors： Umeda H,Nakamura F,Suyama K

更新日期：2001-01-01 00:00:00

abstract：:Bacillus stearothermophilus was grown at the optimal temperature range (center, 65 degrees C), below it (48 and 55 degrees C), and above it (68 degrees C), in a complex medium with or without 2.5 mM Ca2+. The Ca(2+)-supplement improves growth at sub- and supraoptimal temperatures and extends it to higher temperatures ...

journal_title：Archives of biochemistry and biophysics

authors： Jurado AS,Pinheiro TJ,Madeira VM

更新日期：1991-08-15 00:00:00

abstract：:Kirsten murine sarcoma virus-transformed Balb/c 3T3 cells (KiMSV) are highly tumorigenic and metastatic in the appropriate murine host, are loosely adherent to the tissue culture substratum, and can be readily detached from the substratum by ethylene glycol bis(beta-aminoethyl ether) N,N'-tetraacetic acid treatment le...

journal_title：Archives of biochemistry and biophysics

authors： Miketo LJ,Culp LA

更新日期：1983-03-01 00:00:00

abstract：:Phosphatidylinositol (PtdIns) and many other phospholipids activated calmodulin (CaM)-dependent phosphatase in the presence or absence of Ca2+, and the stimulation was more pronounced in the presence of Ca2+. In addition, PtdIns modulated the response of phosphatase to CaM: at low and nonstimulatory concentrations (le...

journal_title：Archives of biochemistry and biophysics

authors： Huang SL,Merat D,Cheung WY

更新日期：1989-04-01 00:00:00

abstract：:The cytochromes P450 are capable of oxidizing a variety of xenobiotics. Binding of a small molecule heteroactivator to a P450 can alter the coupling of substrate oxidation during P450 catalysis, but the degree to which coupling or shunting via one of the three catalytic cycle branch points is linked to the heteroactiv...

journal_title：Archives of biochemistry and biophysics

authors： Locuson CW,Gannett PM,Tracy TS

更新日期：2006-05-15 00:00:00

abstract：:Carcinoembryonic antigen-related cell adhesion molecules (CEACAMs) belong to the immunoglobulin superfamily and contribute to cell-cell adhesion and signal modulation in various tissues. In humans, several CEACAMs are targeted by pathogenic bacteria. One peculiar member of this family, CEACAM3, is exclusively expresse...

journal_title：Archives of biochemistry and biophysics

authors： Buntru A,Roth A,Nyffenegger-Jann NJ,Hauck CR

更新日期：2012-08-01 00:00:00

abstract：:Chloride ion is found to be an essential factor in photoreactivation of the oxygen-evolving center. Tris-washed, 2,6-dichlorophenol indophenol-treated grana are low in both Mn content and oxygen-evolving activity. These grana can restore high oxygen-evolving activity, however, by incorporating Mn2+ ion under weak ligh...

journal_title：Archives of biochemistry and biophysics

authors： Yamashita T,Ashizawa A

更新日期：1985-05-01 00:00:00

abstract：:Based on previous findings of increased nitric oxide synthase (NOS) expression in human gliomas (4), we hypothesized that peroxynitrite, a highly reactive metabolite of nitric oxide (NO) and superoxide (O(*-)(2)), might be increased in these tumors in vivo. Here we demonstrate that nitrotyrosine (a footprint of peroxy...

journal_title：Archives of biochemistry and biophysics

authors： Cobbs CS,Samanta M,Harkins LE,Gillespie GY,Merrick BA,MacMillan-Crow LA

更新日期：2001-10-15 00:00:00

abstract：:Flavin-dependent monooxygenases catalyze a wide variety of redox reactions in important biological processes and are responsible for the synthesis of highly complex natural products. Although much has been learned about FMO chemistry in the last ~80 years of research, several aspects of the reactions catalyzed by thes...

journal_title：Archives of biochemistry and biophysics

authors： Reis RAG,Li H,Johnson M,Sobrado P

更新日期：2021-01-16 00:00:00

abstract：:The hexapeptide Hyp-Gly-Pro-Lys-Gly-Glu was synthesized as a potential substrate for collagen lysyl hydroxylase. Kinetic data on the interaction of this peptide with purified chicken embryo lysyl hydroxylase showed that the hexapeptide is a moderately good substrate having Km, Vmax, and Kcat/Km values comparable to th...

journal_title：Archives of biochemistry and biophysics

authors： Ananthanarayanan VS,Saint-Jean A,Jiang P

更新日期：1992-10-01 00:00:00

abstract：:The conformational behavior of receptor-bound acetylcholine (ACh) was investigated by molecular dynamics simulations. Based on the great similarity among muscarinic receptors, the study was focused on the human M(1), M(2), and M(5) receptors as previously modeled by us. The results showed that receptor-bound ACh was n...

journal_title：Archives of biochemistry and biophysics

authors： Vistoli G,Pedretti A,Testa B,Matucci R

更新日期：2007-08-01 00:00:00

abstract：:The role of malnutrition in the development of cachexia in rats bearing the Yoshida ascites hepatoma AH-130 was investigated by comparing the changes in tissue protein turnover in these animals with those observed in pair-fed controls. The tumor elicited in rats an early and conspicuous loss of body weight and tissue ...

journal_title：Archives of biochemistry and biophysics

authors： Tessitore L,Costelli P,Bonetti G,Baccino FM

更新日期：1993-10-01 00:00:00

abstract：:Lipoxygenase is suggested to be involved in the early event of atherosclerosis by inducing plasma low-density lipoprotein (LDL) oxidation in the subendothelial space of the arterial wall. Since flavonoids such as quercetin are recognized as lipoxygenase inhibitors and they occur mainly in the glycoside form, we assess...

journal_title：Archives of biochemistry and biophysics

authors： Luiz da Silva E,Tsushida T,Terao J

更新日期：1998-01-15 00:00:00

abstract：:Flavopiridol has been shown to induce cell cycle arrest and apoptosis in various tumor cells in vitro and in vivo. Using immobilized flavopiridol, we identified glycogen phosphorylases (GP) from liver and brain as flavopiridol binding proteins from HeLa cell extract. Purified rabbit muscle GP also bound to the flavopi...

journal_title：Archives of biochemistry and biophysics

authors： Kaiser A,Nishi K,Gorin FA,Walsh DA,Bradbury EM,Schnier JB

更新日期：2001-02-15 00:00:00

abstract：:Serratia marcescens metalloprotease inhibitor (SmaPI) is a proteinase inhibitor toward Serratia marcescens metalloprotease (SMP). In sequential deletion analysis of the N-terminal region of the SmaPI, SmaPIs starting at Ser-2 and Leu-3 residues, respectively, had nearly a full inhibitory activity toward SMP. However, ...

journal_title：Archives of biochemistry and biophysics

authors： Bae KH,Kim IC,Kim KS,Shin YC,Byun SM

更新日期：1998-04-01 00:00:00

abstract：:Transforming growth factor-beta (TGF-beta) reportedly induces vascular endothelial growth factor (VEGF) synthesis in osteoblast-like MC3T3-E1 cells. We have recently shown that TGF-beta activates p44/p42 mitogen-activated protein (MAP) kinase and p38 MAP kinase in these cells. In the present study, we investigated the...

journal_title：Archives of biochemistry and biophysics

authors： Tokuda H,Hatakeyama D,Akamatsu S,Tanabe K,Yoshida M,Shibata T,Kozawa O

更新日期：2003-07-01 00:00:00