当前位置： SCI文献检索 > ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS期刊下所有文献 > Structural basis for thermostability revealed through the identification and characterization of a highly thermostable phosphotriesterase-like lactonase from Geobacillus stearothermophilus.

Structural basis for thermostability revealed through the identification and characterization of a highly thermostable phosphotriesterase-like lactonase from Geobacillus stearothermophilus.

Abstract：

:A new enzyme homologous to phosphotriesterase was identified from the bacterium Geobacillus stearothermophilus (GsP). This enzyme belongs to the amidohydrolase family and possesses the ability to hydrolyze both lactone and organophosphate (OP) compounds, making it a phosphotriesterase-like lactonase (PLL). GsP possesses higher OP-degrading activity than recently characterized PLLs, and it is extremely thermostable. GsP is active up to 100 degrees C with an energy of activation of 8.0 kcal/mol towards ethyl paraoxon, and it can withstand an incubation temperature of 60 degrees C for two days. In an attempt to understand the thermostability of PLLs, the X-ray structure of GsP was determined and compared to those of existing PLLs. Based upon a comparative analysis, a new thermal advantage score and plot was developed and reveals that a number of different factors contribute to the thermostability of PLLs.

journal_name

Arch Biochem Biophys

journal_title

Archives of biochemistry and biophysics

authors

Hawwa R,Aikens J,Turner RJ,Santarsiero BD,Mesecar AD

doi

10.1016/j.abb.2009.06.005

subject

Has Abstract

pub_date

2009-08-15 00:00:00

pages

109-20

issue

2

eissn

0003-9861

issn

1096-0384

pii

S0003-9861(09)00182-9

journal_volume

488

pub_type

杂志文章

相关文献

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS文献大全
  • Dependence of exogenous SERCA gene expression on coxsackie adenovirus receptor levels in neonatal and adult cardiac myocytes.

    abstract：:We demonstrate that the efficiency of adenovirus-assisted exogenous Ca(2+) ATPase (SERCA) and reporter (EGFP) gene expression is much higher in primary cultures of myocytes from neonatal rat hearts, than in primary cultures of myocytes from adult rat hearts. In this respect, the neonatal myocytes behave similarly to t...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/s0003-9861(03)00258-3

    authors： Sumbilla C,Ma H,Seth M,Inesi G

    更新日期：2003-07-15 00:00:00

  • Peptaibols as a model for the insertions of chemical modifications.

    abstract：:Peptaibols are linear non ribosomal peptides which have been the object of intense research efforts regarding their synthesis and the elucidation of the mechanism allowing their insertion in biological membranes. Forty years after their discovery they are still considered as model compounds and suitable probes for the...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章,评审

    doi：10.1016/j.abb.2018.09.016

    authors： Das S,Ben Haj Salah K,Djibo M,Inguimbert N

    更新日期：2018-11-15 00:00:00

  • The bacterial hemoglobin from Vitreoscilla can support the aerobic growth of Escherichia coli lacking terminal oxidases.

    abstract：:Two Escherichia coli mutants that lack both cytochrome o and d terminal oxidases are able to grow with glucose as the carbon source but not with the aerobic substrates succinate or lactate. One of these, GV101, is a deletion mutant of cytochrome o and a point mutation of cytochrome d. The other, GK100, is a total dele...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(92)90391-9

    authors： Dikshit RP,Dikshit KL,Liu YX,Webster DA

    更新日期：1992-03-01 00:00:00

  • Both PA63 and PA83 are endocytosed within an anthrax protective antigen mixed heptamer: a putative mechanism to overcome a furin deficiency.

    abstract：:Anthrax toxin consists of protective antigen (PA), and lethal (LF) and edema (EF) factors. A 83 kDa PA monomer (PA83) precursor binds to the cell receptor. Furin-like proprotein convertases (PCs) cleave PA83 to generate cell-bound 63 kDa protein (PA63). PA63 oligomerizes to form a ring-shaped heptamer that binds LF-EF...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2005.11.013

    authors： Chekanov AV,Remacle AG,Golubkov VS,Akatov VS,Sikora S,Savinov AY,Fugere M,Day R,Rozanov DV,Strongin AY

    更新日期：2006-02-01 00:00:00

  • Relationships between the rate of synthesis of ATP and the concentrations of reactants and products of ATP hydrolysis in maize root tips, determined by 31P nuclear magnetic resonance.

    abstract：:Using 31P NMR spectroscopy, we have measured the rate of ATP synthesis, and the free concentrations of ATP, ADP, cytoplasmic Pi, and H+ in maize root tips under a wide range of conditions. We show that the ratio [ATP]/[ADP] in normoxic root tips is greater than 25. We found no simple relationship between the concentra...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(85)90080-3

    authors： Roberts JK,Lane AN,Clark RA,Nieman RH

    更新日期：1985-08-01 00:00:00

  • UDP-galactose 4-epimerase from Escherichia coli: formation of catalytic site during reversible folding.

    abstract：:UDP-galactose 4-epimerase from Escherichia coli is a homodimer of molecular weight 39 kDa/subunit having noncovalently bound NAD acting as cofactor. Denaturation by 8 M urea at pH 7.0 causes 85% loss of its secondary structure and dissociation of its constituent molecules. Dilution of the denaturant by buffer at pH 8....

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.2001.2380

    authors： Barat B,Bhattacharyya D

    更新日期：2001-07-15 00:00:00

  • Two chlorophyll-binding subunits of the photosystem 2 reaction center complex isolated from the thermophilic cyanobacterium Synechococcus sp.

    abstract：:The reaction center of photosystem 2 has been highly purified from digitonin-solubilized thylakoid membranes of the thermophilic cyanobacterium Synechococcus sp. by means of sucrose density gradient centrifugation and electrophoresis on polyacrylamide gels containing digitonin. Sodium dodecyl sulfate-polyacrylamide ge...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(83)90096-6

    authors： Yamagishi A,Katoh S

    更新日期：1983-09-01 00:00:00

  • Differential inhibition of fungal amd mammalian squalene epoxidases by the benzylamine SDZ SBA 586 in comparison with the allylamine terbinafine.

    abstract：:The allylamine class of antifungal compounds are specific inhibitors of squalene epoxidase (SE). However, depending on their chemical structure, allylamine derivatives can be highly selective for either fungal or mammalian SEs. All allylamines tested previously, irrespective of their selectivity, inhibit fungal SEs in...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1997.9908

    authors： Favre B,Ryder NS

    更新日期：1997-04-15 00:00:00

  • Activation of the Ca2+-ATPase of human erythrocyte membrane by an endogenous Ca2+-dependent neutral protease.

    abstract：:Limited proteolysis of the plasma membrane calcium transport ATPase (Ca2+-ATPase) from human erythrocytes by trypsin produces a calmodulin-like activation of its ATP hydrolytic activity and abolishes its calmodulin sensitivity. We now demonstrate a similar kind of activation of the human erythrocyte membrane Ca2+-ATPa...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(88)90498-5

    authors： Wang KK,Villalobo A,Roufogalis BD

    更新日期：1988-02-01 00:00:00

  • Characterization of a full-length, active-site mutant of diphtheria toxin.

    abstract：:A full-length recombinant mutant of diphtheria toxin containing serine in place of a crucial active-site glutamate has been purified and characterized. The serine substitution caused a minor structural alteration in the toxin as measured by trypsinolysis. ADP-ribosyltransferase activity and cytotoxicity of the mutant ...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(92)90626-8

    authors： O'Keefe DO

    更新日期：1992-08-01 00:00:00

  • Redundant enhancement of mouse constitutive androstane receptor transactivation by p160 coactivator family members.

    abstract：:Constitutive androstane receptor (CAR) transactivation is enhanced by p160 coactivators, which include three members, SRC-1, SRC-2, and SRC-3. Each of the p160 coactivators enhanced mouse CAR (mCAR) transactivation of the CYP2B1 phenobarbital (PB)-responsive enhancer in transfected cultured cells and mouse hepatocytes...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2007.09.005

    authors： Xia J,Liao L,Sarkar J,Matsumoto K,Reddy JK,Xu J,Kemper B

    更新日期：2007-12-01 00:00:00

  • An intrinsic ATPase activity of phospho-MEK-1 uncoupled from downstream ERK phosphorylation.

    abstract：:We have developed a highly sensitive assay of MEK-mediated ATP hydrolysis by coupling the formation of ADP to NADH oxidation through the enzymes pyruvate kinase and lactate dehydrogenase. Robust ATP hydrolysis is catalyzed by phosphorylated MEK in the absence of the protein substrate ERK. This ERK-uncoupled ATPase act...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2007.04.004

    authors： Rominger CM,Schaber MD,Yang J,Gontarek RR,Weaver KL,Broderick T,Carter L,Copeland RA,May EW

    更新日期：2007-08-01 00:00:00

  • Prostaglandin H synthase-1: evaluation of C-terminus function.

    abstract：:The first committed step in prostaglandin biosynthesis is catalyzed by prostaglandin H synthase, an enzyme localized to the endoplasmic reticulum (ER) membrane in a variety of cells. Several types of C-terminal region peptide sequence motifs have been found to lead to ER retention of other proteins. We have tested the...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1995.1100

    authors： Ren Y,Loose-Mitchell DS,Kulmacz RJ

    更新日期：1995-02-01 00:00:00

  • Minimizing frustration by folding in an aqueous environment.

    abstract：:Although life as we know it evolved in an aqueous medium, the properties of water are not completely understood. In this review, we focus on the role of water in guiding protein folding and stability. Specifically, we discuss the mechanisms of protein folding in an aqueous environment, the effects of water on the fold...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2007.07.007

    authors： Mattos C,Clark AC

    更新日期：2008-01-01 00:00:00

  • Synthesis of NAADP and cADPR in mitochondria.

    abstract：:Here we investigated whether cADPR and NAADP are synthesized in mitochondria. We found that ADPR-cyclase activity is present in mitochondria. In addition, we describe for the first time synthesis of NAADP in this intracellular organelle. ADPR-cyclase activities (V(MAX)) and NAADP synthesis in mitochondria were about 4...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1999.1463

    authors： Liang M,Chini EN,Cheng J,Dousa TP

    更新日期：1999-11-15 00:00:00

  • Degradation of the starch components amylopectin and amylose by barley α-amylase 1: role of surface binding site 2.

    abstract：:Barley α-amylase isozyme 1 (AMY1, EC 3.2.1.1) contains two surface binding sites, SBS1 and SBS2, involved in the degradation of starch granules. The distinct role of SBS1 and SBS2 remains to be fully understood. Mutational analysis of Tyr-380 situated at SBS2 previously revealed that Tyr-380 is required for binding of...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2012.08.005

    authors： Nielsen JW,Kramhøft B,Bozonnet S,Abou Hachem M,Stipp SL,Svensson B,Willemoës M

    更新日期：2012-12-01 00:00:00

  • Cytochrome P4502E (CYP2E) in brain: constitutive expression, induction by ethanol and localization by fluorescence in situ hybridization.

    abstract：:Cytochrome P4502E (P4502E), the major ethanol-inducible P450 metabolizes ethanol to acetaldehyde and bioactivates procarcinogens to ultimate carcinogens. Metabolism of ethanol to acetaldehyde in the brain could be deleterious since it can react with cytoskeletal proteins, forming adducts. In the present study, rats we...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1999.1477

    authors： Upadhya SC,Tirumalai PS,Boyd MR,Mori T,Ravindranath V

    更新日期：2000-01-01 00:00:00

  • The voltage dependent anion channel affects mitochondrial cholesterol distribution and function.

    abstract：:We have observed abnormally high membrane cholesterol levels and a subsequent deficiency of oxidative energy production in mitochondria from cultured Morris hepatoma cells (MH7777). Using cholesterol affinity chromatography and MALDI-TOF Mass Spectrometry, we have identified the voltage dependent anion channel (VDAC) ...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2007.06.012

    authors： Campbell AM,Chan SH

    更新日期：2007-10-15 00:00:00

  • Cloning and expression of the catalytic domain from rat hepatoma H35 cell GDP-fucose:GM1 alpha 1-->2fucosyltransferase, an enzyme which is activated during early stages of chemical carcinogenesis in rat liver.

    abstract：:A ganglioside GM1-specific alpha 1-->2fucosyltransferase is induced during the early stages of chemical carcinogenesis with N-2-acetylaminofluorene (AAF) in rat liver hepatocytes. The induction of this enzyme gives rise to the expression of a fucose-containing ganglioside with the same determinant structure as blood g...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1998.0728

    authors： Sherwood AL,Holmes EH

    更新日期：1998-07-15 00:00:00

  • The asymmetric orientation of cytochrome b561 in bovine chromaffin granule membranes.

    abstract：:The topological arrangement of cytochrome b561 in the bovine adrenal medullary chromaffin granule membrane was investigated by radiolabeling and immunoprecipitation techniques using antibody raised against the purified cytochrome. The first labeling procedure involved a membrane-permeable amino group labeling reagent,...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(84)90074-2

    authors： Duong LT,Fleming PJ

    更新日期：1984-01-01 00:00:00

  • Dietary and hormonal regulation of aldolase B gene transcription in rat liver.

    abstract：:In the liver of the fasted rat, the aldolase B (AldB) mRNA level decreased to about half of that of the control rat. When the control rat was refed the glucose-rich diet, the AldB mRNA level increased about six to seven times more than in the fasted rat. This increase was shown as the activation of the AldB gene trans...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1994.1447

    authors： Gomez PF,Ito K,Huang Y,Otsu K,Kuzumaki T,Ishikawa K

    更新日期：1994-11-01 00:00:00

  • Phospholipase C from Clostridium perfringens: preparation and characterization of homogeneous enzyme.

    abstract：:A new procedure for the purification of phospholipase C from Clostridium perfringens has been devised that results in essentially pure enzyme. The procedure consists of ammonium sulfate fractionation, ion-exchange chromatography on QAE-Sephadex, and affinity chromatography on phosphatidylcholine linked to Sepharose. T...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(84)90403-x

    authors： Krug EL,Kent C

    更新日期：1984-06-01 00:00:00

  • In vivo regulation of phosphorylation level and activity of phosphofructokinase by serotonin in Fasciola hepatica.

    abstract：:The level of phosphorylation and activation of phosphofructokinase by serotonin (5-hydroxytryptamine) was studied in intact liver flukes Fasciola hepatica. The enzyme was immunoprecipitated with antiserum prepared against pure enzyme from the liver flukes. The resuspended immunoprecipitated enzyme retained most of its...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(89)90307-x

    authors： Kamemoto ES,Lan L,Mansour TE

    更新日期：1989-06-01 00:00:00

  • Sequence and antigenicity of calf metallothionein II.

    abstract：:Metallothionein isoform II was purified from calf liver. The protein had a metal content of 1.2-1.9 Cu ions and 5.6-6.2 Zn ions per molecule in different preparations. The complete amino acid sequence of the molecule was determined by automatic Edman degradation of CNBr and tryptic peptides of the carboxymethylated pr...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(86)90212-2

    authors： Winge DR,Gray WR,Zelazowski A,Garvey JS

    更新日期：1986-02-15 00:00:00

  • 15-Keto prostaglandin E2 induces heme oxygenase-1 expression through activation of Nrf2 in human colon epithelial CCD 841 CoN cells.

    abstract：:Prostaglandin E2 (PGE2) plays a key role in inflammation-associated carcinogenesis. NAD+-dependent 15-hydroxyprostaglandin dehydrogenase (15-PGDH) catalyzes the oxidation of the 15(S)-hydroxyl group of PGE2 to generate 15-keto PGE2. 15-PGDH has been known as a tumor suppressor in various malignancies including colon c...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2019.108162

    authors： Lee JE,Zhong X,Lee JY,Surh YJ,Na HK

    更新日期：2020-01-15 00:00:00

  • Carbon-centered free radical intermediates in the hematin- and ram seminal vesicle-catalyzed decomposition of fatty acid hydroperoxides.

    abstract：:Heme-catalyzed decomposition of unsaturated hydroperoxy fatty acids has been proposed to proceed via carbon-centered free radicals (delocalized at positions C11, C12, and C13 for 15-hydroperoxy-eicosatetraenoic acid (15-HPETE). The stable products are usually epoxy fatty acids and epoxy alcohols. Hydroperoxides from a...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(86)90046-9

    authors： Schreiber J,Mason RP,Eling TE

    更新日期：1986-11-15 00:00:00

  • Inhibition of mammalian 15-lipoxygenase-dependent lipid peroxidation in low-density lipoprotein by quercetin and quercetin monoglucosides.

    abstract：:Lipoxygenase is suggested to be involved in the early event of atherosclerosis by inducing plasma low-density lipoprotein (LDL) oxidation in the subendothelial space of the arterial wall. Since flavonoids such as quercetin are recognized as lipoxygenase inhibitors and they occur mainly in the glycoside form, we assess...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1997.0455

    authors： Luiz da Silva E,Tsushida T,Terao J

    更新日期：1998-01-15 00:00:00

  • Detection of a functional xenobiotic response element in a widely employed FoxO-responsive reporter construct.

    abstract：:FHRE-Luc is a promoter reporter construct that is widely used to assess the activity of FoxO (forkhead box, class O) transcription factors. We here demonstrate that this promoter construct responds to exposure of HepG2 human hepatoma cells to known agonists of the aryl hydrocarbon receptor (AhR), 3-methylcholanthrene,...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2011.10.006

    authors： Eckers A,Sauerbier E,Anwar-Mohamed A,Hamann I,Esser C,Schroeder P,El-Kadi AO,Klotz LO

    更新日期：2011-12-15 00:00:00

  • Expression of P4501A1 in a primary culture of rainbow trout hepatocytes exposed to beta-naphthoflavone or 2,3,7,8-tetrachlorodibenzo-p-dioxin.

    abstract：:Primary cultures of rainbow trout hepatocytes were used to study the expression of CYP1A1 mRNA, its protein product (P4501A1), and catalytic activities (7-ethoxy-resorufin-O-deethylase, EROD) during a 96-h period after exposure of cells to beta-naphthoflavone (BNF) or 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Hepato...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(92)90072-5

    authors： Pesonen M,Goksøyr A,Andersson T

    更新日期：1992-01-01 00:00:00

  • Role of cytosolic NAD(P)H-quinone oxidoreductase and alcohol dehydrogenase in the reduction of p-nitrosophenol following chronic ethanol ingestion.

    abstract：:Rats fed an ethanol-containing diet for 4 weeks showed a 3- to 5-fold increase over isocalorically pair-fed controls with respect to cytosolic NAD(P)H-quinone oxidoreductase (NQOR) (E.C.1.6.99.2) with both menadione and dichlorophenol-indophenol as substrates. Rates of NAD(P)H-dependent p-nitrosophenol (pNSP) reductio...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(92)90510-4

    authors： Hajos AK,Winston GW

    更新日期：1992-06-01 00:00:00