Dependence of exogenous SERCA gene expression on coxsackie adenovirus receptor levels in neonatal and adult cardiac myocytes.

abstract：:Excessive iron accumulation in the heart can lead to iron overload cardiomyopathy (IOC), the leading cause of death in hemochromatosis patients. Current understanding regarding the mechanism by which iron overload causes a deterioration in cardiac performance, mitochondrial dysfunction, and impaired mitochondrial dyna...

journal_title：Archives of biochemistry and biophysics

authors： Sumneang N,Siri-Angkul N,Kumfu S,Chattipakorn SC,Chattipakorn N

更新日期：2020-02-15 00:00:00

abstract：:When applied in liquid paraffin to the upper surface of expanding spinach leaves, [1-14C]palmitic acid was efficiently and exclusively incorporated into the sn-1 position of cellular glycerolipids, principally phosphatidylcholine and triacylglycerol. A slow transfer of fatty acids from phosphatidylcholine to chloropla...

journal_title：Archives of biochemistry and biophysics

authors： Roughan PG,Thompson GA Jr,Cho SH

更新日期：1987-12-01 00:00:00

abstract：:Methionine is an essential amino acid for both prokaryotic and eukaryotic organisms; however, little is known concerning its utilization in African trypanosomes, protozoa of the Trypanosoma brucei group. This study explored the Michaelis-Menten kinetic constants for transport and pool formation as well as metabolic ut...

journal_title：Archives of biochemistry and biophysics

authors： Goldberg B,Rattendi D,Lloyd D,Yarlett N,Bacchi CJ

更新日期：2000-05-01 00:00:00

abstract：:A detailed knowledge about the substrate specificities of the heparin lyases is necessary when using these enzymes as tools for elucidating the sequence of heparin and heparan sulfate. The substrate specificity of heparin lyases I, II, and III have been profiled with structurally defined, heparin-derived oligosacchari...

journal_title：Archives of biochemistry and biophysics

authors： Desai UR,Wang HM,Linhardt RJ

更新日期：1993-11-01 00:00:00

abstract：:Conversion of a steroid receptor complex from the 8 S to the 4S form results in new interactions between the steroid and the receptor and/or formation of new intra-protein bonds within the receptor molecule itself. These bonds must be broken before the steroid is released. In order to localize these newly formed inter...

journal_title：Archives of biochemistry and biophysics

authors： Wolfson AJ,Ryu ML,Baghdady R

更新日期：1991-02-01 00:00:00

abstract：:The cytochromes P450 are capable of oxidizing a variety of xenobiotics. Binding of a small molecule heteroactivator to a P450 can alter the coupling of substrate oxidation during P450 catalysis, but the degree to which coupling or shunting via one of the three catalytic cycle branch points is linked to the heteroactiv...

journal_title：Archives of biochemistry and biophysics

authors： Locuson CW,Gannett PM,Tracy TS

更新日期：2006-05-15 00:00:00

abstract：:Much of what is known about the antioxidant mechanism of vitamin E has been learned from studies of lipid dispersions, solutions, or subcellular organelles. We have investigated the effect of vitamin E supplementation on intact live eucaryotic cells. L1210 murine leukemia cells were exposed to an oxidative stress indu...

journal_title：Archives of biochemistry and biophysics

authors： Wagner BA,Buettner GR,Burns CP

更新日期：1996-10-15 00:00:00

abstract：:The effect of high temperature stress on the expression of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activase was examined in wheat (Triticum aestivum L.) leaves, which normally possess 46- and 42-kDa activase forms. Heat stress at 38 degrees C significantly reduced total activase mRNA levels compared ...

journal_title：Archives of biochemistry and biophysics

authors： Law RD,Crafts-Brandner SJ

更新日期：2001-02-15 00:00:00

abstract：:The presence of a trifunctional beta-oxidation complex in pig heart and its relationship to the known long-chain enoyl-CoA hydratase (EC 4.2.1.74) from pig heart mitochondria were investigated. For this study, the complex was partially purified by chromatography on DEAE-cellulose in the absence of detergents and was p...

journal_title：Archives of biochemistry and biophysics

authors： Luo MJ,He XY,Sprecher H,Schulz H

更新日期：1993-07-01 00:00:00

abstract：:Nanoceria were synthesized by discharging plasma at 800 V with a frequency of 30 kHz for 0-25 min using a pulsed unipolar power supply into solutions containing 1 or 2 mM of Ce(NO3)2. UV-Vis spectroscopy showed a characteristic absorbance maxima at 304-320 nm for the nanoceria with increase in the intensity of the pea...

journal_title：Archives of biochemistry and biophysics

authors： Davoodbasha M,Park BR,Rhee WJ,Lee SY,Kim JW

更新日期：2018-05-01 00:00:00

abstract：:Glycosaminoglycan (GAG)-protein interactions regulate a myriad of physiologic and pathologic processes, yet an understanding of how these molecules interact is lacking. The role of the pattern and spacing of basic amino acids (arginine (R) and lysine (K)) in heparin binding sites was investigated using peptide analogs...

journal_title：Archives of biochemistry and biophysics

authors： Fromm JR,Hileman RE,Caldwell EE,Weiler JM,Linhardt RJ

更新日期：1997-07-01 00:00:00

abstract：:Previous experiments performed in recombinant systems have suggested that protein-protein interactions occur between the UGTs and may play a significant role in modulating enzyme activity. However, evidence of UGT protein-protein interactions either in vivo or in more physiologically relevant in vitro systems has yet ...

journal_title：Archives of biochemistry and biophysics

authors： Konopnicki CM,Dickmann LJ,Tracy JM,Tukey RH,Wienkers LC,Foti RS

更新日期：2013-07-15 00:00:00

abstract：:The Arabidopsis thaliana genome contains at least 32 terpenoid synthase (AtTPS) genes [Aubourg et al., Mol. Genet. Genom. 267 (2002) 730] a few of which have recently been characterized. Based on hierarchical cluster analysis of AtTPS gene expression, measured by microarray profiling and validated with published expre...

journal_title：Archives of biochemistry and biophysics

authors： Ro DK,Ehlting J,Keeling CI,Lin R,Mattheus N,Bohlmann J

更新日期：2006-04-15 00:00:00

abstract：:Low-density lipoprotein receptor-related protein (LRP)/alpha 2-macroglobulin receptor is a member of the low-density lipoprotein receptor family. It is known to bind a wide variety of unrelated ligands including alpha 2-macroglobulin-proteinase complexes, tissue plasminogen activator, apolipoprotein E-enriched very lo...

journal_title：Archives of biochemistry and biophysics

authors： Wu SM,Pizzo SV

更新日期：1996-02-01 00:00:00

abstract：:Cytochrome P450 (P450) 2C1/2 contains redundant endoplasmic reticulum (ER) retention signals and is excluded from the recycling pathway. Other P450s, such as P450 2E1, have been detected in the plasma membrane and Golgi apparatus. To examine whether the mechanisms of ER retention might differ for P450 2C1/2 and P450 2...

journal_title：Archives of biochemistry and biophysics

authors： Szczesna-Skorupa E,Chen CD,Kemper B

更新日期：2000-02-15 00:00:00

abstract：:A recent note from our laboratory reported that L-band (1.2 GHz) electron paramagnetic resonance spectroscopy can be utilized in detecting the formation of Cr(V) intermediates from chromate-treated whole mice. Since Cr(V) is thought to be one of the key species in the mechanism of chromate's toxicity, we carried out a...

journal_title：Archives of biochemistry and biophysics

authors： Liu KJ,Shi X,Jiang JJ,Goda F,Dalal N,Swartz HM

更新日期：1995-10-20 00:00:00

abstract：BACKGROUND:This study was designed to identify novel links between lipid species and disease progression in non-alcoholic fatty liver disease (NAFLD). METHODS:We analyzed lipid species in the liver and plasma of db/db mice fed a choline-deficient l-amino acid-defined, high-fat diet (CDAHFD) using liquid chromatography...

journal_title：Archives of biochemistry and biophysics

authors： Horas H Nababan S,Nishiumi S,Kawano Y,Kobayashi T,Yoshida M,Azuma T

更新日期：2017-06-01 00:00:00

abstract：:The oxidation of quercetin by horseradish peroxidase/H(2)O(2) was studied in the absence but especially also in the presence of glutathione (GSH). HPLC analysis of the reaction products formed in the absence of GSH revealed formation of at least 20 different products, a result in line with other studies reporting the ...

journal_title：Archives of biochemistry and biophysics

authors： Awad HM,Boersma MG,Vervoort J,Rietjens IM

更新日期：2000-06-15 00:00:00

abstract：:It was shown previously that the ATP synthase complex of bovine heart mitochondria contains an essential set of thiols or dithiols in its membrane sector (F0), whose modification by various reagents results in uncoupling [Yagi, T., and Hatefi, Y. (1984) Biochemistry 23, 2449-2455]. The sensitivity to modifiers was inc...

journal_title：Archives of biochemistry and biophysics

authors： Yagi T,Hatefi Y

更新日期：1987-04-01 00:00:00

abstract：:The glucose transporter of the bacterial phosphotransferase system mediates sugar transport across the cytoplasmic membrane concomitant with sugar phosphorylation. It consists of a cytoplasmic subunit IIA(Glc) and the transmembrane subunit IICB(Glc). IICB(Glc) was purified to homogeneity by urea/alkali washing of memb...

journal_title：Archives of biochemistry and biophysics

authors： Zhuang J,Gutknecht R,Flükiger K,Hasler L,Erni B,Engel A

更新日期：1999-12-01 00:00:00

abstract：:The mechanism of the biosynthesis of 12-oxo-10,15(Z)-phytodienoic acid (12-oxo-PDA) from 13(S)-hydroperoxy-9(Z),11(E),15(Z)-octadecatrienoic acid in preparations of corn (Zea mays L.) was studied. In the initial reaction the hydroperoxide was converted into an unstable allene oxide, 12,13(S)-epoxy-9(Z),11,15(Z)-octade...

journal_title：Archives of biochemistry and biophysics

authors： Hamberg M,Fahlstadius P

更新日期：1990-02-01 00:00:00

abstract：:Sensitive assays were developed for long chain and very long chain fatty acid oxidation in human skin fibroblast homogenates. Stearic and lignoceric acids were degraded by the fibroblasts by the beta-oxidation pathway. The cofactor requirements for stearic and lignoceric acid beta-oxidation were very similar but not i...

journal_title：Archives of biochemistry and biophysics

authors： Singh H,Poulos A

更新日期：1986-10-01 00:00:00

abstract：:Iowa trypanosome antigen type (IaTat) 1.2 variant surface glycoprotein (VSG) is synthesized in vitro as a Mr 54,000 preprotein that contains a 31-amino-acid signal peptide. Translation of mRNA in the presence of either dog pancreas or trypanosome microsomal membranes results in cotranslational cleavage of the signal p...

journal_title：Archives of biochemistry and biophysics

authors： Presper KA,Heath EC

更新日期：1986-04-01 00:00:00

abstract：:Binding of ligands to the catalytic center of mammalian triosephosphate isomerase (TPI) induces a conformational change(s) that enhances the specific deamidation of Asn71 at the subunit interface. Deamidation initiates dissociation and degradation of the enzyme in vivo and in vitro. We have utilized circular dichroism...

journal_title：Archives of biochemistry and biophysics

authors： Sun AQ,Yüksel KU,Rao GS,Gracy RW

更新日期：1992-06-01 00:00:00

abstract：:Many of the compounds considered for use in pharmaceutical formulations demonstrate incompatibilities with other components at high enough concentrations, including pairs of polymers, polymers and salts, or even proteins in combination with polymers, salts, or other proteins. Freeze concentration can force solutions i...

journal_title：Archives of biochemistry and biophysics

authors： Heller MC,Carpenter JF,Randolph TW

更新日期：1999-03-15 00:00:00

abstract：:In this study, the binding points of MethB and two structurally-related cationic phenoxazine dyes [meldola blue (MB) and nile blue (NB)] to human butyrylcholinesterase (BChE) were investigated by molecular docking and site directed mutagenesis. The comparative inhibitory effects of MethB, MB and NB on recombinant wild...

journal_title：Archives of biochemistry and biophysics

authors： Sezgin Z,Biberoglu K,Chupakhin V,Makhaeva GF,Tacal O

更新日期：2013-04-01 00:00:00

abstract：:Three alanine aminotransferases, two minor (AlaAT-1, AlaAT-3) and one major (AlaAT-2), were detected by native gel electrophoresis of leaf extracts from Panicum miliaceum L. AlaAT-2 was purified to homogeneity and a specific polyclonal antibody was raised against it which did not react with the other two forms of the ...

journal_title：Archives of biochemistry and biophysics

authors： Son D,Jo J,Sugiyama T

更新日期：1991-09-01 00:00:00

abstract：:The plant-derived flavonoid, quercetin (QCT), has many biological actions, including cardioprotective actions, resulting from its antioxidant and anti-inflammatory effects. In this study, effects of QCT and its metabolites on the contraction and Ca2+ transients (CaT) of mouse single cardiomyocytes were simultaneously ...

journal_title：Archives of biochemistry and biophysics

authors： Hayamizu K,Morimoto S,Nonaka M,Hoka S,Sasaguri T

更新日期：2018-01-01 00:00:00

abstract：:The pH dependence of the activity of Cu,Zn superoxide dismutases from bovine erythrocytes and shark liver was studied by pulse radiolysis in both the native enzymes and those chemically modified at lysine side chains. The study was aimed at identifying the residues responsible for the activity decrease at pH > 9, obse...

journal_title：Archives of biochemistry and biophysics

authors： Polticelli F,O'Neill P,Costanzo S,Lania A,Rotilio G,Desideri A

更新日期：1995-08-01 00:00:00

abstract：:Mitochondrial permeability transition (MPT) is a Ca(2+)-dependent, cyclosporine A-sensitive, non-selective inner membrane permeabilization induced by a wide range of agents or conditions, which has often been associated with necrotic or apoptotic cell death. When mitochondria isolated from livers of rats treated with ...

journal_title：Archives of biochemistry and biophysics

authors： Andreu GL,Delgado R,Velho JA,Curti C,Vercesi AE

更新日期：2005-07-15 00:00:00