Purification and characterization of alanine aminotransferase from Panicum miliaceum leaves.


:Three alanine aminotransferases, two minor (AlaAT-1, AlaAT-3) and one major (AlaAT-2), were detected by native gel electrophoresis of leaf extracts from Panicum miliaceum L. AlaAT-2 was purified to homogeneity and a specific polyclonal antibody was raised against it which did not react with the other two forms of the enzyme. The enzyme, with an apparent molecular size of 102 kDa, appeared to be a dimer of a single 50-kDa polypeptide. The enzyme has a relatively broad pH optima with similar curves for the forward and reverse directions, ranging between 6.5 and 7.5. The Km values of this enzyme were 6.67, 0.15, 5.00, and 0.33 mM for alanine, 2-oxoglutarate, glutamate, and pyruvate, respectively. The activity of AlaAT-2 was found to increase markedly during leaf greening in parallel with the increase of immunochemically titrated protein, and it is suggested to function in the C4 photosynthetic cycle.


Arch Biochem Biophys


Son D,Jo J,Sugiyama T




Has Abstract


1991-09-01 00:00:00














  • Role of cytochrome b5 in NADH-dependent microsomal reduction of ferric complexes, lipid peroxidation, and hydrogen peroxide generation.

    abstract::The NADH-dependent microsomal electron transfer system consists of NADH-cytochrome b5 reductase and cytochrome b5, which donates reducing equivalents to fatty acyl desaturase, cytochrome P450, and other reactions. A study was carried out to investigate the interaction of NADH with several ferric complexes and to evalu...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Yang MX,Cederbaum AI

    更新日期:1995-12-20 00:00:00

  • Normal mode analysis with molecular geometry restraints: bridging molecular mechanics and elastic models.

    abstract::A new method for normal mode analysis is reported for all-atom structures using molecular geometry restraints (MGR). Similar to common molecular mechanics force fields, the MGR potential contains short- and long-range terms. The short-range terms are defined by molecular geometry, i.e., bond lengths, angles and dihedr...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Lu M,Ma J

    更新日期:2011-04-01 00:00:00

  • Heterologous expression and characterization of an active lignin peroxidase from Phanerochaete chrysosporium using recombinant baculovirus.

    abstract::The cDNA clone lambda ML-1 encoding one of the extracellular lignin peroxidases from the white rot fungus, Phanerochaete chrysosporium, was heterologously expressed in an active form using a recombinant baculovirus system. The glycosylated extracellular form of the recombinant protein contained the ferriprotoporphyrin...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Johnson TM,Li JK

    更新日期:1991-12-01 00:00:00

  • Function of several critical amino acids in human pyruvate dehydrogenase revealed by its structure.

    abstract::Pyruvate dehydrogenase (E1), an alpha(2)beta(2) tetramer, catalyzes the oxidative decarboxylation of pyruvate and reductive acetylation of lipoyl moieties of the dihydrolipoamide acetyltransferase. The roles of betaW135, alphaP188, alphaM181, alphaH15, and alphaR349 of E1 determined by kinetic analysis were reassessed...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Korotchkina LG,Ciszak EM,Patel MS

    更新日期:2004-09-15 00:00:00

  • Ethanol exposure decreases mitochondrial outer membrane permeability in cultured rat hepatocytes.

    abstract::Mitochondrial metabolism depends on movement of hydrophilic metabolites through the mitochondrial outer membrane via the voltage-dependent anion channel (VDAC). Here we assessed VDAC permeability of intracellular mitochondria in cultured hepatocytes after plasma membrane permeabilization with 8 microM digitonin. Block...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Holmuhamedov E,Lemasters JJ

    更新日期:2009-01-15 00:00:00

  • Effects of sulfhydryl reagents, retinoids, and solubilization on the activity of microsomal retinol dehydrogenase.

    abstract::A microsomal retinol dehydrogenase (RoDH) that recognizes holo-cellular retinol binding protein (CRBP) as substrate is inhibited by phenylarsine oxide (IC50 = 3 microM) in the presence of 10 mM cysteine. Inhibition was reversible with dithiothreitol, indicating that two cysteine residues in close proximity are essenti...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Boerman MH,Napoli JL

    更新日期:1995-08-20 00:00:00

  • Hydroxyamino acid specificity of smooth muscle myosin light chain kinase.

    abstract::Synthetic peptides corresponding to the phosphorylation site in the myosin regulatory light chain from smooth muscle, Lys-Lys-Arg-Ala-Arg-Ala-Thr-Ser-Asn-Val-Phe-Ala ([Ala14,15]MLC(11-23] and containing a variety of hydroxyamino acid analogs at position 19, were tested as substrates for the smooth muscle myosin light ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Pearson RB,Floyd DM,Hunt JT,Lee VG,Kemp BE

    更新日期:1988-01-01 00:00:00

  • Low temperature plasma induces angiogenic growth factor via up-regulating hypoxia-inducible factor 1α in human dermal fibroblasts.

    abstract::Numerous studies on the application of low temperature plasma (LTP) have produced impressive results, including antimicrobial, antitumor, and wound healing effects. Although LTP research has branched out to include medical applications, the detailed effects and working mechanisms of LTP on wound healing have not been ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Cui HS,Joo SY,Lee DH,Yu JH,Jeong JH,Kim JB,Seo CH

    更新日期:2017-09-15 00:00:00

  • A pre-steady state and steady state kinetic analysis of the N-ribosyl hydrolase activity of hCD157.

    abstract::hCD157 catalyzes the hydrolysis of nicotinamide riboside (NR) and nicotinic acid riboside (NAR). The release of nicotinamide or nicotinic acid from NR or NAR was confirmed by spectrophotometric, HPLC and NMR analyses. hCD157 is inactivated by a mechanism-based inhibitor, 2'-deoxy-2'-fluoro-nicotinamide arabinoside (fN...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Preugschat F,Carter LH,Boros EE,Porter DJ,Stewart EL,Shewchuk LM

    更新日期:2014-12-15 00:00:00

  • The oxidizing power of the glutathione thiyl radical as measured by its electrode potential at physiological pH.

    abstract::The oxidizing power of the thiyl radical (GS*) produced on oxidation of glutathione (GSH) was determined as the mid-point electrode potential (reduction potential) of the one-electron couple E(m)(GS*,H+/GSH) in water, as a function of pH over the physiological range. The method involved measuring the equilibrium const...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Madej E,Wardman P

    更新日期:2007-06-01 00:00:00

  • Structural studies on glycoprotein oligosaccharides of chromaffin granule membranes and dopamine beta-hydroxylase.

    abstract::Dopamine beta-hydroxylase present in the soluble matrix of bovine adrenal medullary chromaffin granules contains biantennary complex oligosaccharides and high-mannose oligosaccharides in a molar ratio of approximately 2:1. The high-mannose oligosaccharides contain an average of six mannose residues. The largest biante...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Margolis RK,Finne J,Krusius T,Margolis RU

    更新日期:1984-02-01 00:00:00

  • Trimethyllead acetate: a first-choice heavy atom derivative for protein crystallography.

    abstract::The three-dimensional conformation of a protein provides a wealth of biochemical information and with the advent of cloning techniques that allow the preparation of proteins almost at will, a renewed interest has arisen in the crystallographic determination of protein structures. As in any research technique, however,...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Holden HM,Rayment I

    更新日期:1991-11-15 00:00:00

  • Functional reconstitution of prokaryote and eukaryote membrane proteins.

    abstract::A new strategy for the functional reconstitution of membrane proteins is described. This approach introduces a new class of protein stabilizing agents--osmolytes--whose presence at high concentration (10-20%) during detergent solubilization prevents the inactivations that normally occur when proteins are extracted fro...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Maloney PC,Ambudkar SV

    更新日期:1989-02-15 00:00:00

  • A residue-level investigation of the equilibrium unfolding of the C2A domain of synaptotagmin 1.

    abstract::Fibroblast growth factor (FGF) 1 is known to be released in response to cellular stress conditions through formation of a multi-protein complex with synaptotagmin 1 and S100A13. In this study, we characterized the denaturant-induced unfolding of synaptotagmin 1, C2A domain in a residue-specific manner by NMR spectrosc...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Anbazhagan V,Wang HM,Lu CS,Yu C

    更新日期:2009-10-15 00:00:00

  • BH3-only proteins trigger cytochrome c release, but how?

    abstract::The mitochondrial apoptosis pathway has been neatly ordered. Mitochondrial apoptosis is governed by Bcl-2 family proteins, and their respective contributions determine the release of cytochrome c. It is clear that, among the Bcl-2 family, BH3-only proteins are the triggers: activation of BH3-only proteins by apoptotic...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章,评审


    authors: Häcker G,Weber A

    更新日期:2007-06-15 00:00:00

  • HemITAM signaling by CEACAM3, a human granulocyte receptor recognizing bacterial pathogens.

    abstract::Carcinoembryonic antigen-related cell adhesion molecules (CEACAMs) belong to the immunoglobulin superfamily and contribute to cell-cell adhesion and signal modulation in various tissues. In humans, several CEACAMs are targeted by pathogenic bacteria. One peculiar member of this family, CEACAM3, is exclusively expresse...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章,评审


    authors: Buntru A,Roth A,Nyffenegger-Jann NJ,Hauck CR

    更新日期:2012-08-01 00:00:00

  • Efflux mechanism and pathway of verapamil pumping by human P-glycoprotein.

    abstract::Multidrug resistance (MDR) caused by overexpressed permeability-glycoprotein (P-gp) in cancer cells is the main barrier for the cure of cancers. P-gp can pump many chemotherapeutic drugs, which is a viable target to overcome P-gp-mediated MDR by efficient inhibitors of P-gp. However, limited understanding of the efflu...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Wang L,Sun Y

    更新日期:2020-12-15 00:00:00

  • Vitamin D improves pulmonary function in a rat model for congenital diaphragmatic hernia.

    abstract::A congenital diaphragmatic hernia (CDH) is an anomaly caused by defects in the diaphragm; the resulting limited thorax cavity in turn restricts lung growth (pulmonary hypoplasia). This condition is related to pulmonary hypertension. Despite advances in neonatal CDH therapy, the mortality for severe pulmonary hypoplasi...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Ito Y,Tsuda H,Imai K,Miki R,Miura M,Tachi A,Tano S,Hirako-Takamura S,Moriyama Y,Ushida T,Kobayashi T,Sumigama S,Kajiyama H,Kikkawa F,Kotani T

    更新日期:2021-01-20 00:00:00

  • Identification, characterization, and partial purification of 4 alpha-carboxysterol-C3-dehydrogenase/ C4-decarboxylase from Zea mays.

    abstract::A microsomal preparation from seedlings of Zea mays catalyzed the NAD+-dependent oxidative decarboxylation of several substrates, including 4alpha-carboxy-cholest-7-en-3beta-ol, synthesized according to a new procedure, giving the first in vitro evidence for this enzymatic activity in a higher plant. A GC assay has be...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Rondet S,Taton M,Rahier A

    更新日期:1999-06-15 00:00:00

  • Translocation of deltaPKC to mitochondria during cardiac reperfusion enhances superoxide anion production and induces loss in mitochondrial function.

    abstract::Activation of the delta-isoform of protein kinase C (deltaPKC) by certain conditions of oxidative stress results in translocation of the kinase to the mitochondria leading to release of cytochrome c and the induction of apoptosis. In the current study, the effects of myocardial reperfusion-induced deltaPKC translocati...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Churchill EN,Szweda LI

    更新日期:2005-07-15 00:00:00

  • Where is the vitamin D receptor?

    abstract::The vitamin D receptor (VDR) is a member of the nuclear receptor superfamily and plays a central role in the biological actions of vitamin D. VDR regulates the expression of numerous genes involved in calcium/phosphate homeostasis, cellular proliferation and differentiation, and immune response, largely in a ligand-de...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章,评审


    authors: Wang Y,Zhu J,DeLuca HF

    更新日期:2012-07-01 00:00:00

  • Characterization of a novel carboxypeptidase produced by the entomopathogenic fungus Metarhizium anisopliae.

    abstract::Preparative isoelectric focusing and gel filtration chromatography were used to purify a carboxypeptidase produced by the entomopathogenic fungus Metarhizium anisopliae during growth on cockroach cuticle. The enzyme was inhibited by diisopropyl fluorophosphate, implying involvement of a serine residue in catalysis. Ho...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: St Leger RJ,Bidochka MJ,Roberts DW

    更新日期:1994-11-01 00:00:00

  • The photosynthetic deficiency due to puhC gene deletion in Rhodobacter capsulatus suggests a PuhC protein-dependent process of RC/LH1/PufX complex reorganization.

    abstract::Optimal photosynthetic reaction centre (RC) and core antenna (LH1) levels in the purple bacterium Rhodobacter capsulatus require the puhC gene. Deletion of puhC had little effect on RC and LH1 assembly individually, but significantly inhibited the photosynthetic growth of RC+ LH1- strains, suggesting that maximal RC c...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Aklujkar M,Prince RC,Beatty JT

    更新日期:2006-10-01 00:00:00

  • Effect of dexamethasone and phenobarbital on run-on transcription rate and CYP3A mRNA concentration in rat liver: changes during development.

    abstract::Modulation of CYP3A1 and CYP3A2 mRNA expression by dexamethasone and by phenobarbital has been studied in immature (21-day-old) and adult (90-day-old) rat liver. Positive modulation of these forms by both agents markedly declines with the age of the animals. However, CYP3A2 mRNA, although physiologically extinguished ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Telhada MB,Pereira TM,Lechner MC

    更新日期:1992-11-01 00:00:00

  • Binding of soluble myelin basic protein to various conformational forms of alpha2-macroglobulin.

    abstract::Myelin basic protein is known to be released into the circulation following traumatic injuries or demyelination within the central nervous system, resulting in the generation of potentially immunogenic myelin basic protein material. In this investigation we have studied the binding of bovine and human myelin basic pro...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Gunnarsson M,Jensen PE

    更新日期:1998-11-15 00:00:00

  • Crystal structure and immunogenicity of the class C acid phosphatase from Pasteurella multocida.

    abstract::Pasteurella multocida is a pathogen of veterinary and medical importance. Here, we report the 1.85Å resolution crystal structure of the class C acid phosphatase from this organism (denoted rPmCCAP). The structure shows that rPmCCAP exhibits the same haloacid dehalogenase fold and dimeric assembly as the class C enzyme...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Singh H,Malinski TJ,Reilly TJ,Henzl MT,Tanner JJ

    更新日期:2011-05-01 00:00:00

  • 12-O-tetradecanoylphorbol-13-acetate induces apoptosis in renal epithelial cells through a growth signal conflict which is prevented by activated ras.

    abstract::12-O-Tetradecanoylphorbol-13-acetate (TPA) induced apoptosis in the pig renal epithelial cell line LLC-PK1 after 24 h of treatment as assessed by caspase 3 activation. Cotreatment of the cells with bryostatin markedly reduced the apoptotic effects of TPA. Okadaic acid, another tumor promoter, also induced apoptosis. E...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Lee RG,Rosson D

    更新日期:2001-01-15 00:00:00

  • Glycosphingolipids of human plasma.

    abstract::A number of glycosphingolipids, including 10 gangliosides, not previously identified in human plasma have been characterized. The plasma contains 2 micrograms of lipid-bound sialic acid/ml plasma and 54% of the gangliosides are monosialo, 30% disialo, 10% trisialo, and 6% tetrasialo. Individual glycosphingolipids were...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Kundu SK,Diego I,Osovitz S,Marcus DM

    更新日期:1985-05-01 00:00:00

  • Relationship between oxidative stress and heme oxygenase induction by copper sulfate.

    abstract::The effect of copper sulfate (CuSO4) on both hepatic oxidative stress and heme oxygenase induction was studied. A strong increase in in vivo rat liver chemiluminescence was observed 1 h after Cu(II) administration. To evaluate liver antioxidant enzymatic defenses, superoxide dismutase, catalase, and glutathione peroxi...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Ossola JO,Groppa MD,Tomaro ML

    更新日期:1997-01-15 00:00:00

  • The molecular weight and oligomerization of rabbit thrombomodulin as assessed by sedimentation equilibrium.

    abstract::Lubrol-solubilized rabbit thrombomodulin has been examined by equilibrium sedimentation in buffers that include sufficient D2O to make the detergent neutrally buoyant. Data were acquired at rotor speeds from 12,000 to 28,000 rpm from two thrombomodulin preparations, at protein concentrations from 0.01 to 0.07%, and in...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Winnard PT,Esmon CT,Laue TM

    更新日期:1989-02-15 00:00:00