Abstract:
:Iowa trypanosome antigen type (IaTat) 1.2 variant surface glycoprotein (VSG) is synthesized in vitro as a Mr 54,000 preprotein that contains a 31-amino-acid signal peptide. Translation of mRNA in the presence of either dog pancreas or trypanosome microsomal membranes results in cotranslational cleavage of the signal peptide and addition of core oligosaccharide side chains to the protein. Analysis of these products on sodium dodecyl sulfate (SDS)-gels indicates that removal of the signal peptide (Mr 3200) is almost exactly compensated for by an increase in molecular weight due to carbohydrate addition. Pulse-chase experiments in cultures of isolated trypanosomes indicate that two IaTat 1.2 VSG species (Mr 58,000 and 60,000) occur in vivo. When glycosylation is inhibited by incubation of trypanosomes with tunicamycin, a single Mr 50,000 polypeptide is immunoprecipitated. The multiple protein species, therefore, arise from heterogeneity in carbohydrate side chains whose synthesis and transfer to the protein are tunicamycin sensitive. Sequence analysis verified that both species of VSG contain identical amino-terminal sequences. Further post-translational processing of IaTat 1.2 VSG includes addition of phosphate and myristic acid residues, both of which have been shown to be located in the immunologically cross-reactive determinant at the carboxyl terminus of the protein. Exposure of this attachment site requires post-translational proteolytic removal of a 17-amino-acid peptide from the carboxyl terminus of an intermediate form of VSG.
journal_name
Arch Biochem Biophysjournal_title
Archives of biochemistry and biophysicsauthors
Presper KA,Heath ECdoi
10.1016/0003-9861(86)90492-3subject
Has Abstractpub_date
1986-04-01 00:00:00pages
460-8issue
1eissn
0003-9861issn
1096-0384pii
0003-9861(86)90492-3journal_volume
246pub_type
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journal_title:Archives of biochemistry and biophysics
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更新日期:2007-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:2011-12-01 00:00:00
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journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
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更新日期:2018-10-15 00:00:00
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pub_type: 杂志文章
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更新日期:1995-12-20 00:00:00
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pub_type: 杂志文章
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更新日期:1992-08-01 00:00:00