Abstract：
:The Metabotropic glutamate receptor 2 (mGluR2) is involved in several neurological and psychiatric disorders and is an attractive drug target. It is believed to form a strict dimer and the dimeric assembly is necessary for glutamate induced activation. Although many studies have focused on glutamate induced conformational changes, the dimerization propensity of mGluR2 with and without glutamate has never been investigated. Also, the role of the unstructured loop in dimerization of mGluR2 is not clear. Here, using Forster Resonance Energy Transfer (FRET) based assay in live cells we show that mGluR2 does not form a "strict dimer" rather it exists in a dynamic monomer-dimer equilibrium. The unstructured loop moderately destabilizes the dimers. Furthermore, binding of glutamate to mGluR2 induces conformational change that promotes monomerization of mGluR2. In the absence of an unstructured loop, mGluR2 neither undergoes conformational change nor monomerizes upon binding to glutamate.
journal_name
Arch Biochem Biophysjournal_title
Archives of biochemistry and biophysicsauthors
Singh DR,Pandey K,Mishra AK,Pandey P,Vivcharuk Vdoi
10.1016/j.abb.2020.108632subject
Has Abstractpub_date
2021-01-15 00:00:00pages
108632eissn
0003-9861issn
1096-0384pii
S0003-9861(20)30641-Xjournal_volume
697pub_type
杂志文章abstract：:Providing clean and affordable drinking water without harmful disinfection byproducts generated by conventional chemical disinfectants gives rise to the need for technological innovation. Nanotechnology has great potential in purifying water and wastewater treatment. A graphene oxide-silver (GO-Ag) nanocomposite with ...
journal_title：Archives of biochemistry and biophysics
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journal_title：Archives of biochemistry and biophysics
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doi：10.1016/0003-9861(92)90024-q
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pub_type： 杂志文章,评审
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doi：10.1016/j.abb.2005.05.001
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journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
doi：10.1006/abbi.1995.1236
更新日期：1995-04-20 00:00:00
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pub_type： 杂志文章
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更新日期：2009-01-01 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：1986-07-01 00:00:00
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pub_type： 杂志文章
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journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
doi：10.1006/abbi.2000.2043
更新日期：2000-11-15 00:00:00
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journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
doi：10.1016/j.abb.2013.10.006
更新日期：2013-12-01 00:00:00
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journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
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更新日期：2011-05-15 00:00:00
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journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
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更新日期：1986-06-01 00:00:00
