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High temperature stress increases the expression of wheat leaf ribulose-1,5-bisphosphate carboxylase/oxygenase activase protein.

Abstract：

:The effect of high temperature stress on the expression of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activase was examined in wheat (Triticum aestivum L.) leaves, which normally possess 46- and 42-kDa activase forms. Heat stress at 38 degrees C significantly reduced total activase mRNA levels compared to controls, and recovery of activase transcription was only marginal 24 h after alleviating heat stress. In contrast to transcript abundance, immunoblot analysis indicated that heat stress increased the accumulation of the 42-kDa activase and induced a putative 41-kDa form. Heat stress did not affect the amounts of the 46- and 42-kDa activase forms (present as 51- and 45-kDa preproteins) recovered after their immunoprecipitation from in vitro translation products. De novo protein synthesis in vivo in the presence of [35S]Met/Cys showed an increase in the amount of newly synthesized 42-kDa subunit after 4 h of heat stress, and synthesis of the putative 41-kDa activase was apparent. In contrast to activase, heat stress led to a rapid and large reduction in the de novo synthesis of the large and small subunits of Rubisco. Long-term (48-h) heat stress further increased the amounts of de novo synthesized 42- and 41-kDa activase forms. After 24 h of recovery from heat stress, de novo synthesis of the 42-kDa activase returned to control levels, while a small amount of 41-kDa protein was still expressed. Southern analysis suggested the presence of a single activase gene. These results indicate that heat stress alters activase expression, most likely posttranscriptionally, and suggest that the heat-induced expression of the 42- and 41-kDa subunits of wheat leaf Rubisco activase may be related to the maintenance and acclimation of photosynthetic CO2 fixation during high temperature stress in wheat.

journal_name

Arch Biochem Biophys

journal_title

Archives of biochemistry and biophysics

authors

Law RD,Crafts-Brandner SJ

doi

10.1006/abbi.2000.2225

subject

Has Abstract

pub_date

2001-02-15 00:00:00

pages

261-7

issue

eissn

0003-9861

issn

1096-0384

pii

S0003-9861(00)92225-2

journal_volume

386

pub_type

杂志文章

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High temperature stress increases the expression of wheat leaf ribulose-1,5-bisphosphate carboxylase/oxygenase activase protein.