Abstract：
:Diglycyl triperoxodivanadate [V2O2(O2)3(Gly H)2(H2O)2], a synthetic compound with mu-peroxo-bridge derived from H2O2 and vanadate, oxidized bromide to a bromination-competent intermediate in phosphate buffer and physiological pH. This is in contrast to the requirement of acid medium with H2O2 as the oxidant. Addition of its solid to bromide solution instantly produced a 262-nm-absorbing compound that converted phenol red (a trap) to its 592-nm-absorbing bromo-derivative. The high bromination activity was lost on dissolving this compound in water and the solution showed the presence of peroxovanadates (mono and di) and vanadates (V1 and oligomeric V10) in 51V-NMR spectrum. Of these, diperoxovanadate and vanadate together supported slow bromination activity by a second set of reactions including bromide-assisted reductive formation of vanadyl. Bromination activity dependent on vanadyl was sensitive to oxidation by excess H2O2 and to complexation by EDTA, whereas that of triperoxodivanadate was relatively insensitive. Vanadyl and diperoxovanadate are capable of forming a mu-peroxo-bridged complex that is essentially similar to the synthetic vanadate dimer used in the present experiments. It appears that a mu-peroxo-intermediate is the proximal oxidant of bromide in vanadium-catalyzed bromoperoxidation.
journal_name
Arch Biochem Biophysjournal_title
Archives of biochemistry and biophysicsauthors
Rao AV,Islam NS,Ramasarma Tdoi
10.1006/abbi.1997.0103subject
Has Abstractpub_date
1997-06-15 00:00:00pages
289-97issue
2eissn
0003-9861issn
1096-0384pii
S0003-9861(97)90103-Xjournal_volume
342pub_type
杂志文章abstract：:Glycerol phosphate acyltransferase (GPAT) catalyzes the formation of 1-acyl-sn-glycerol-3-phosphate from glycerol-3-phosphate and long chain fatty acyl-CoA substrates. We previously determined the topography of the mitochondrial GPAT1 isoform (mtGPAT1, 828 amino acids). mtGPAT1 has two transmembrane domains (TMDs) (aa...
journal_title：Archives of biochemistry and biophysics
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更新日期：1997-04-15 00:00:00
