Functionally conservative substitutions at cardiac troponin I S43/45.

abstract：:The isolation and purification of a 21,000-Da (pI 4.9) novel protein from porcine anterior pituitary and whole human pituitary is described. Comparison of the NH2-terminal sequence of the first 77 and 81 residues of the human and porcine homologs shows only one conservative substitution at residue 12, namely an Ala fo...

journal_title：Archives of biochemistry and biophysics

authors： Seidah NG,Hsi KL,De Serres G,Rochemont J,Hamelin J,Antakly T,Cantin M,Chrétien M

更新日期：1983-09-01 00:00:00

abstract：:We have shown that superoxide (O2.-) is produced during the oxidation of veratryl alcohol by lignin peroxidase (LiP) by the reaction of the veratryl alcohol cation radical with hydrogen peroxide (D. B. Barr, M. M. Shah, and S. D. Aust, 1993, J. Biol. Chem. 268, 241-244). Compound III, an inactive form of peroxidases c...

journal_title：Archives of biochemistry and biophysics

authors： Barr DP,Aust SD

更新日期：1994-06-01 00:00:00

abstract：:Protein tyrosine kinase Yes is a cellular homolog of v-Yes, the oncogenic protein product of avian sarcoma virus Y73. Yes is a member of the Src family and its activation has been associated with several types of human cancer. Human Yes has not been previously characterized enzymatically. To carry out biochemical char...

journal_title：Archives of biochemistry and biophysics

authors： Sun G,Budde RJ

更新日期：1997-09-01 00:00:00

abstract：:It has been demonstrated that lysosomal membrane proteins are directed to lysosomes by a tyrosine-containing structural motif in their cytoplasmic tails. It is presently unclear whether lysosomal membrane proteins are directly transported to lysosomes or first taken to the plasma membrane and then directed to the lyso...

journal_title：Archives of biochemistry and biophysics

authors： Carlsson SR,Fukuda M

更新日期：1992-08-01 00:00:00

abstract：:Functional concerns regarding hemoglobin-based red cell substitutes have generally centered on two parameters: (a) oxygen binding and delivery properties and (b) stabilization of the hemoglobin tetramer to prevent dimerization. Strategic chemical cross-linking and site-directed mutagenesis have produced proteins that ...

journal_title：Archives of biochemistry and biophysics

authors： Cashon RE,Alayash AI

更新日期：1995-01-10 00:00:00

abstract：:Reactive oxygen species like hydrogen peroxide (H2O2) have been shown to serve as messengers in the induction of NF-kappa B and, hence, in the activation and replication of human immunodeficiency virus type 1 (HIV-1) in human cells. Several antioxidant compounds and iron chelators have been shown to interfere with bot...

journal_title：Archives of biochemistry and biophysics

authors： Sappey C,Boelaert JR,Legrand-Poels S,Grady RW,Piette J

更新日期：1995-08-01 00:00:00

abstract：:Synephrinase, an enzyme catalyzing the conversion of (-)-synephrine into p-hydroxyphenylacetaldehyde and methylamine, was purified to apparent homogeneity from the cell-free extracts of Arthrobacter synephrinum grown on (+/-)-synephrine as the sole source of carbon and nitrogen. A 40-fold purification was sufficient t...

journal_title：Archives of biochemistry and biophysics

authors： Manne V,Kutty KR,Pillarisetti SR

更新日期：1986-07-01 00:00:00

abstract：:Leukotoxin (ltx) and isoleukotoxin (iltx) methyl esters, are metabolites of methyl linoleic acid, an essential fatty acid. They have been associated with acute respiratory distress syndrome. The observed toxicity of ltx and iltx is, in fact, due to the metabolism of the epoxides to their corresponding diols by soluble...

journal_title：Archives of biochemistry and biophysics

authors： Greene JF,Williamson KC,Newman JW,Morisseau C,Hammock BD

更新日期：2000-04-15 00:00:00

abstract：:Glycosylphosphatidylinositol transamidase (GPI-T) catalyzes the post-translational addition of the GPI anchor to the C-terminus of some proteins. In most eukaryotes, Gpi8, the active site subunit of GPI-T, is part of a hetero-pentameric complex containing Gpi16, Gaa1, Gpi17, and Gab1. Gpi8, Gaa1, and Gpi16 co-purify a...

journal_title：Archives of biochemistry and biophysics

authors： Gamage DG,Varma Y,Meitzler JL,Morissette R,Ness TJ,Hendrickson TL

更新日期：2017-11-01 00:00:00

abstract：:Using Escherichia coli guanosine triphosphate cyclohydrolase, dihydroneopterin triphosphate was synthesized from guanosine triphosphate and was compared with sepiapterin as a substrate for tetrahydrobiopterin formation in bovine adrenal medulla extracts. The dihydrofolate reductase inhibitor, methotrexate, blocks the ...

journal_title：Archives of biochemistry and biophysics

authors： Smith GK,Nichol CA

更新日期：1983-11-01 00:00:00

abstract：:The molecular basis for the unique activities of rabbit cytochrome P450 2B5, compared with the highly related rabbit 2B4, was investigated using hybrid enzymes and site-directed mutagenesis. Alterations in androstenedione hydroxylase profiles observed with 2B4-2B5 hybrids expressed in COS cells showed that key amino a...

journal_title：Archives of biochemistry and biophysics

authors： Szklarz GD,He YQ,Kedzie KM,Halpert JR,Burnett VL

更新日期：1996-03-15 00:00:00

abstract：:Nonspecific lipid transfer protein (sterol carrier protein2) has previously been proposed to function as (i) a catalyst for intracellular movement of newly synthesized phospholipid, (ii) a cofactor in the biosynthesis and metabolism of cholesterol, and (iii) a cofactor in the feedback inhibition of cholesterol synthes...

journal_title：Archives of biochemistry and biophysics

authors： Crain RC,Clark RW

更新日期：1985-08-15 00:00:00

abstract：:Three forms of 20-kDa myosin light chain (MLC), unphosphorylated, monophosphorylated, and diphosphorylated MLC (designated 20K, 20K-P, and 20K-PP) were demonstrated in thrombin-stimulated human platelets by two different gel electrophoretic methods: in the presence of glycerol urea or in two dimensions (isoelectric an...

journal_title：Archives of biochemistry and biophysics

authors： Naka M,Saitoh M,Hidaka H

更新日期：1988-03-01 00:00:00

abstract：:Macrophages play a pivotal role in the defense response against harmful pathogens and stimuli by releasing various pro-inflammatory mediators. However, overproduction of pro-inflammatory mediators will do harm to the organism and cause inflammation-associated diseases. Omentin-1, which is a newly discovered adipokine,...

journal_title：Archives of biochemistry and biophysics

authors： Wang J,Gao Y,Lin F,Han K,Wang X

更新日期：2020-01-15 00:00:00

abstract：:Mitochondrial permeability transition (MPT) is a Ca(2+)-dependent, cyclosporine A-sensitive, non-selective inner membrane permeabilization induced by a wide range of agents or conditions, which has often been associated with necrotic or apoptotic cell death. When mitochondria isolated from livers of rats treated with ...

journal_title：Archives of biochemistry and biophysics

authors： Andreu GL,Delgado R,Velho JA,Curti C,Vercesi AE

更新日期：2005-07-15 00:00:00

abstract：:A novel bioluminescence-based solid-phase assay is described for the enzyme GDPFuc:Gal(beta)1-3GlcNAc (Fuc to GlcNAc) alpha1,4-fucosyltransferase (alpha1,4FT), which generates the Lewis a blood group antigen (Le(a)) (Gal(beta)1-3[Fuc(alpha)1-4]GlcNAc-R). Lacto-N-tetraose (LNT,Ga ta)1-3GlcNAc(beta)1-3Gal(beta)1-4Glc) w...

journal_title：Archives of biochemistry and biophysics

authors： DeBose-Boyd RA,Nyame AK,Smith DF,Cummings RD

更新日期：1996-11-01 00:00:00

abstract：:Complete primary structures of five allergenically active oligosaccharitols (HPG-beta 2-N5a, -N6, -N7a, -N7b, and -N9) derived from a sea squirt H-antigen were studied. Structural characterization was carried out by a new method in which products of limited periodate oxidation, followed by derivatization with p-aminob...

journal_title：Archives of biochemistry and biophysics

authors： Ohta M,Matsuura F,Kobayashi Y,Shigeta S,Ono K,Oka S

更新日期：1991-11-01 00:00:00

abstract：:Under a variety of conditions, the permeability of the inner mitochondrial membrane to small solutes can be nonselectively increased. A classic mitochondrial permeability transition (MPT) was originally identified based on its dependence on matrix Ca2+ and its extreme sensitivity to cyclosporin A (CsA). It is now clea...

journal_title：Archives of biochemistry and biophysics

authors： Kushnareva YE,Haley LM,Sokolove PM

更新日期：1999-03-01 00:00:00

abstract：:Excessive iron accumulation in the heart can lead to iron overload cardiomyopathy (IOC), the leading cause of death in hemochromatosis patients. Current understanding regarding the mechanism by which iron overload causes a deterioration in cardiac performance, mitochondrial dysfunction, and impaired mitochondrial dyna...

journal_title：Archives of biochemistry and biophysics

authors： Sumneang N,Siri-Angkul N,Kumfu S,Chattipakorn SC,Chattipakorn N

更新日期：2020-02-15 00:00:00

abstract：:The putrescine analogue 1,4-diamino-2-butanone (DAB) is highly toxic to various microorganisms, including Trypanosoma cruzi. Similar to other α-aminocarbonyl metabolites, DAB exhibits pro-oxidant properties. DAB undergoes metal-catalyzed oxidation yielding H(2)O(2), NH(4)(+) ion, and a highly toxic α-oxoaldehyde. In v...

journal_title：Archives of biochemistry and biophysics

authors： Soares CO,Colli W,Bechara EJ,Alves MJ

更新日期：2012-12-15 00:00:00

abstract：:Cystathionine beta-lyase, the second enzyme of the transsulfuration pathway leading to homocysteine synthesis was purified over 16,000-fold from spinach (Spinacia oleracea L.) leaf chloroplasts (soluble fraction). Enzyme activity was followed along the purification scheme by either a colorimetric method for the determ...

journal_title：Archives of biochemistry and biophysics

authors： Droux M,Ravanel S,Douce R

更新日期：1995-01-10 00:00:00

abstract：:Adenylate cyclase activation by GTP and octopamine as well as basal activity (in the presence of Mg2+) have been studied as a function of membrane structure in plasma membranes from brain of the dipterous Ceratitis capitata. Benzyl alcohol and lidocaine, but not phenobarbital, inhibited the three activities to the sam...

journal_title：Archives of biochemistry and biophysics

authors： Guillen A,Haro A,Gavilanes FG,Municio AM

更新日期：1991-05-01 00:00:00

abstract：:Cellobiose dehydrogenase (CDH), an extracellular hemoflavoenzyme produced by the cellulose-degrading cultures of Phanerochaete chrysosporium, oxidizes cellobiose to cellobionolactone. CDH has been purified to homogeneity by a five-step purification procedure. The homogeneous CDH is monomeric and has a relative molecul...

journal_title：Archives of biochemistry and biophysics

authors： Bao W,Usha SN,Renganathan V

更新日期：1993-02-01 00:00:00

abstract：:Hemorrhagic toxin f (HT-f) was isolated from Crotalus atrox (Western Diamondback Rattlesnake) venom by a five-step purification procedure. Homogeneity was established by the formation of a single band in acrylamide gel electrophoresis, isoelectric focusing, and sodium dodecyl sulfate (SDS)-electrophoresis. HT-f has a ...

journal_title：Archives of biochemistry and biophysics

authors： Nikai T,Mori N,Kishida M,Sugihara H,Tu AT

更新日期：1984-06-01 00:00:00

abstract：:The complete amino acid sequence of Rapana thomasiana hemocyanin functional unit RtH2-e was determined by direct sequencing and matrix-assisted laser desorption ionization mass spectrometry of peptides obtained by cleavage with EndoLysC proteinase, chymotrypsin, and trypsin. The single-polypeptide chain of RtH2-e cons...

journal_title：Archives of biochemistry and biophysics

authors： Stoeva S,Idakieva K,Betzel C,Genov N,Voelter W

更新日期：2002-03-15 00:00:00

abstract：:The solution structure of native and systematically modified ovine submaxillary mucin (OSM) has been probed by proton NMR spectroscopic methods. Most of the resonances in the spectra have been tentatively assigned to the peptide and O-linked disaccharide, alpha-N-acetylneuraminic acid 2----6 alpha-N-acetylgalactosamin...

journal_title：Archives of biochemistry and biophysics

authors： Gerken TA

更新日期：1986-06-01 00:00:00

abstract：:To evaluate the conformation of reduced HEL, the monoclonal antibodies HyC1 and HyC2, which recognize different conformational epitopes on native hen egg lysozyme (HEL), were used, and the kinetics of their interactions with native HEL, S-1,2-dicarboxyethylated HEL (DCE-HEL), and carboxymethylated Cys6 and Cys127 HEL ...

journal_title：Archives of biochemistry and biophysics

authors： Oda M,Kitai A,Murakami A,Nishimura M,Ohkuri T,Abe Y,Ueda T,Nakamura H,Azuma T

更新日期：2010-02-15 00:00:00

abstract：:Human programmed cell death 5 (PDCD5) is a translocatory protein playing an important role in the apoptotic process of cells. Although there are accumulated data about PDCD5 function, the correlation of the structure with the function of PDCD5 has not been investigated. Here, we report the studies of structure-functio...

journal_title：Archives of biochemistry and biophysics

authors： Yao H,Xu L,Feng Y,Liu D,Chen Y,Wang J

更新日期：2009-06-15 00:00:00

abstract：:We previously reported that administration of dexamethasone (DEX) and other selected pharmacological agents to rats resulted in a profound increase in hepatic cytochrome P450 3A1 in both sexes, but male constitutive P450 3A2 was modestly increased (4-fold) in adult males and not detected in either treated or untreated...

journal_title：Archives of biochemistry and biophysics

authors： Ghosal A,Sadrieh N,Reik L,Levin W,Thomas PE

更新日期：1996-08-01 00:00:00

abstract：:The study aimed to evaluate the impact of adenosine receptors (ARs) on human colon tumor cells (HCT 116, HT-29) growth and sensitivity to 5-Fluorouracil (5-FU) an anticancer chemotherapeutic drug. The exposure of cancer cells to a selective A(3)-AR agonist (IB-MECA) resulted in an increase in HT-29 cells number, where...

journal_title：Archives of biochemistry and biophysics

authors： Sakowicz-Burkiewicz M,Kitowska A,Grden M,Maciejewska I,Szutowicz A,Pawelczyk T

更新日期：2013-05-01 00:00:00