Superoxide from glucose oxidase or from nitroblue tetrazolium?

abstract：:Dethiolation of proteins (reduction of protein mixed disulfides) by NADPH-dependent and glutathione (GSH)-dependent enzymes, and by nonenzymatic reaction with GSH, was studied by electrofocusing methodology with glycogen phosphorylase b and creatine kinase as substrates. Phosphorylase b was not rapidly dethiolated by ...

journal_title：Archives of biochemistry and biophysics

authors： Park EM,Thomas JA

更新日期：1989-10-01 00:00:00

abstract：:Vitamin E (vE) is a biological free radical scavenger capable of providing antioxidant protection depending upon its tissue content. In previous studies, we observed that vE increased significantly in rat lungs after oxidant exposure, and we postulated that vE may be mobilized to the lung from other body sites under o...

journal_title：Archives of biochemistry and biophysics

authors： Elsayed NM,Mustafa MG,Mead JF

更新日期：1990-11-01 00:00:00

abstract：:A neutral proteolytic activity that converts human Big endothelin-1 (Big Et-1) to endothelin-1 has been identified from a human endothelial hybrid cell line, EAHY 926. This enzyme is an integral membrane protein and cofractionates with other enzymes typically found in the plasma membrane. The activity has been solubil...

journal_title：Archives of biochemistry and biophysics

authors： Waxman L,Doshi KP,Gaul SL,Wang S,Bednar RA,Stern AM

更新日期：1994-01-01 00:00:00

abstract：:After an energy source shift-down, Escherichia coli accumulates 70 S ribosome-mRNA complexes ("70 S monosomes"). The monosome mRNA strands are predominantly primary transcription products with purine nucleoside 5'-triphosphate and 5'-diphosphate termini present at a 1:2 ratio. The number-average chain length is 564 +/...

journal_title：Archives of biochemistry and biophysics

authors： Wnek AP,Jacobson LA

更新日期：1985-08-15 00:00:00

abstract：:Glycine residues are recognized as important structural determinants in nucleotide-binding domains of many enzymes. The functional significance of seven glycine residues invariant in all 22 eNTPDase sequences was therefore examined. Glycine-to-alanine mutants of eNTPDase3 were analyzed for nucleotidase activities and ...

journal_title：Archives of biochemistry and biophysics

authors： Kirley TL,Yang F,Ivanenkov VV

更新日期：2001-11-01 00:00:00

abstract：:Germinated seeds of barley, after anaerobic treatment, produce considerable amounts of hydrogen. The anaerobic stress induces hydrogenase activity, as measured by the ability to evolve hydrogen from reduced methyl viologen. Several pieces of evidence, including bacteriological controls and induction in axenic calluses...

journal_title：Archives of biochemistry and biophysics

authors： Torres V,Ballesteros A,Fernández VM

更新日期：1986-02-15 00:00:00

abstract：:It has previously been found that there is a synergistic effect of free purine bases and low concentrations of dialyzed platelet extract on net synthesis of DNA in serum-starved fibroblast-like mouse L-cells. Experiments with a mutant line of L-cells that was deficient in hypoxanthine phosphoribosyl transferase (EC 2....

journal_title：Archives of biochemistry and biophysics

authors： Flodgaard H,Klenow H

更新日期：1985-06-01 00:00:00

abstract：:cDNA for mouse cytochrome c oxidase subunit IV (COXIV) was isolated by screening mouse liver and kidney cDNA libraries with a bovine COXIV cDNA probe. The 679-nucleotide nearly full length cDNA codes for a 22-amino acid presequence and a 147-amino acid mature protein which show 77 to 95% positional identity with the p...

journal_title：Archives of biochemistry and biophysics

authors： Carter RS,Avadhani NG

更新日期：1991-07-01 00:00:00

abstract：:The severe acute respiratory syndrome coronavirus papain-like protease (SARS-CoV PLpro) carries out N-terminal processing of the viral replicase polyprotein, and also exhibits Lys48-linked polyubiquitin chain debranching and ISG15 precursor processing activities in vitro. Here, we used SDS-PAGE and fluorescence-based ...

journal_title：Archives of biochemistry and biophysics

authors： Lindner HA,Lytvyn V,Qi H,Lachance P,Ziomek E,Ménard R

更新日期：2007-10-01 00:00:00

abstract：:The extrahepatic expression of UDP-glucuronosyltransferases (UGTs) is important in the detoxification of a number of endogenous and exogenous compounds, including 5-hydroxytryptamine and morphine. Studies were designed to investigate the extrahepatic expression of human UGTs using RT-PCR techniques and to determine th...

journal_title：Archives of biochemistry and biophysics

authors： King CD,Rios GR,Assouline JA,Tephly TR

更新日期：1999-05-01 00:00:00

abstract：:Mutations in MYBPC3, the gene encoding cardiac myosin binding protein C (cMyBP-C), are a major cause of hypertrophic cardiomyopathy (HCM). While most mutations encode premature stop codons, missense mutations causing single amino acid substitutions are also common. Here we investigated effects of a single proline for ...

journal_title：Archives of biochemistry and biophysics

authors： van Dijk SJ,Bezold Kooiker K,Mazzalupo S,Yang Y,Kostyukova AS,Mustacich DJ,Hoye ER,Stern JA,Kittleson MD,Harris SP

更新日期：2016-07-01 00:00:00

abstract：:Methylglyoxal (MGO), a reactive dicarbonyl metabolite is a potent arginine directed glycating agent which has implications for diabetes-related complications. Dicarbonyl metabolites are produced endogenously and in a state of misbalance, they contribute to cell and tissue dysfunction through protein and DNA modificati...

journal_title：Archives of biochemistry and biophysics

authors： Abidi M,Khan MS,Ahmad S,Kausar T,Nayeem SM,Islam S,Ali A,Alam K,Moinuddin

更新日期：2018-05-01 00:00:00

abstract：:CYP119 from Sulfolobus solfataricus is the first well-characterized thermophilic cytochrome P450 enzyme. The endogenous substrate for this enzyme is not known but it hydroxylates lauric acid in a reaction supported by surrogate mesophilic electron donors. However, reconstitution of a high-temperature catalytic system ...

journal_title：Archives of biochemistry and biophysics

authors： Puchkaev AV,Ortiz de Montellano PR

更新日期：2005-02-01 00:00:00

abstract：:The topographical localization of the N-terminus of cytochrome b in the inner mitochondrial membrane was determined by mild proteolysis of the yeast mitochondrial cytochrome bc1 complex and identification of the proteolytic fragments derived from subunits of the complex with an established orientation in the inner mem...

journal_title：Archives of biochemistry and biophysics

authors： Beattie DS,Jenkins HC,Howton MM

更新日期：1994-07-01 00:00:00

abstract：:The activities, properties, and steady-state kinetics of the five enzymes catalyzing the synthesis of 1-acyl- and 1-alkyl-sn-glycerol 3-phosphate in the cultured skin fibroblasts from Zellweger syndrome patients and normal controls were studied in detail. Judging from their Km and Vmax values, glycerol phosphate acylt...

journal_title：Archives of biochemistry and biophysics

authors： Webber KO,Datta NS,Hajra AK

更新日期：1987-05-01 00:00:00

abstract：:Studies were carried out at pH 7.0 and gamma/2 0.15 before addition of CaCl2 or EDTA. Clotting time, tau, at 3.03 microM fibrinogen and 0.91 u/ml thrombin was determined for equilibrium systems. With added Ca2+, tau decreases, from tau 0 at 0 added Ca2+ (mean, 29.7 +/- 3 s), by approximately 3 s at 5 mM added Ca2+. Wi...

journal_title：Archives of biochemistry and biophysics

authors： Perizzolo KE,Sullivan S,Waugh DF

更新日期：1985-03-01 00:00:00

abstract：:The regulation of phosphofructokinase during development of C2C12 myoblasts to myotubes was investigated. Enzyme activity was markedly increased during myogenic development. The increase was observed when enzyme activity was measured under optimal conditions and was not due to changes in the allosteric kinetic propert...

journal_title：Archives of biochemistry and biophysics

authors： Wills KN,Mansour TE

更新日期：1990-04-01 00:00:00

abstract：:p53, a tumor suppressor and transcription factor, is a critical modulator in the cellular response to stress. Exposure of glutathione-deficient GCS-2 cells to arsenite significantly phosphorylated and stabilized p53. In addition, p53 transcriptionally repressed Hsp90beta gene expression. Mutation analysis revealed a p...

journal_title：Archives of biochemistry and biophysics

authors： Habib GM

更新日期：2009-01-01 00:00:00

abstract：:Plant 3-hydroxy-3-methylglutaryl-CoA reductase(HMGR; EC 1.1.1.34) and sucrose-phosphate synthase (SPS; EC 2.4.1.14) and synthetic peptides designed from the known phosphorylation sites of plant HMGR (SAMS*: KSHMKYNRSTKDVK), rat acetyl-CoA carboxylase (SAMS: HMRSAMSGLHLVKRR), spinach SPS (SP2: GRRJRRISSVEJJDKK), and sp...

journal_title：Archives of biochemistry and biophysics

authors： Toroser D,Huber SC

更新日期：1998-07-15 00:00:00

abstract：:Cancer osaka thyroid (COT), a human MAP 3 K, is essential for lipopolysaccharide activation of the Erk MAPK cascade in macrophages. COT 30--467 is insoluble, whereas low levels of COT 30--397 can be expressed, but this protein is unstable. However, both COT 30--467 and COT 30--397 are expressed in a soluble and stable...

journal_title：Archives of biochemistry and biophysics

authors： Jia Y,Quinn CM,Bump NJ,Clark KM,Clabbers A,Hardman J,Gagnon A,Kamens J,Tomlinson MJ,Wishart N,Allen H

更新日期：2005-09-01 00:00:00

abstract：:Phosphofructokinase was purified from muscle tissue of two different molluscs, edible snails, Helix pomatia (gastropoda), and mussels, Mytilus edulis (bivalvia). Under denaturing conditions, both enzymes had a molecular mass of 82 kDa. In the presence of ATP-Mg2+, the enzymes were rapidly phosphorylated in vitro by th...

journal_title：Archives of biochemistry and biophysics

authors： Biethinger M,Hoffmann R,Hofer HW

更新日期：1991-06-01 00:00:00

abstract：:The enzyme phenylalanine hydroxylase catalyzes the hydroxylation of excess phenylalanine in the liver to tyrosine. The enzyme is regulated allosterically by phenylalanine and by phosphorylation of Ser16. Hydrogen/deuterium exchange monitored by mass spectrometry has been used to gain insight into any structural change...

journal_title：Archives of biochemistry and biophysics

authors： Li J,Fitzpatrick PF

更新日期：2013-07-15 00:00:00

abstract：:Hyaluronan controls keratinocyte proliferation and regeneration. We examined effect of UV on the expression of hyaluronan synthases (HASs) and hyaluronidases in cultured normal human newborn foreskin epidermal keratinocytes, NHEK(F). HAS3 mRNA was expressed predominantly and HAS2 mRNA expressed in lesser amounts and b...

journal_title：Archives of biochemistry and biophysics

authors： Kakizaki I,Itano N,Kimata K,Hanada K,Kon A,Yamaguchi M,Takahashi T,Takagaki K

更新日期：2008-03-01 00:00:00

abstract：:Previous work from this laboratory indicated that the N-terminus of recombinant human cytochrome P450 (P450) 1A2 expressed in Escherichia coli is blocked (P. Sandhu, Z. Guo, T. Baba, M. V. Martin, R. H. Tukey, and F. P. Guengerich, (1994) Arch. Biochem. Biophys. 30, 168 -177). A modification of this construct was done...

journal_title：Archives of biochemistry and biophysics

authors： Dong MS,Yamazaki H,Guo Z,Guengerich FP

更新日期：1996-03-01 00:00:00

abstract：:Linamarase (EC 3.2.1.21) was purified from cassava petiole, stem, and root cortex by ammonium sulfate precipitation, column chromatography on Sepharose 6B, and chromatofocusing. The last step resolved the enzyme from each source into three forms with pI values of 4.3, 3.3, and 2.9. Each form was found to be oligomeric...

journal_title：Archives of biochemistry and biophysics

authors： Eksittikul T,Chulavatnatol M

更新日期：1988-10-01 00:00:00

abstract：:Casein kinase 1 phosphorylated rabbit skeletal muscle glycogen synthase at both seryl and threonyl residues. With glycogen synthase phosphorylated up to 7.5 mol phosphate/mol subunit, about 26% of the phosphate was present in the N-terminal cyanogen bromide fragment (CB1) and 74% in the C-terminal fragment (CB2). Both...

journal_title：Archives of biochemistry and biophysics

authors： Guasch MD,Plana M,Vila J,Itarte E

更新日期：1986-11-01 00:00:00

abstract：:The role of the conserved sequence motif 301DDLTDP306 in the F0F1 ATP synthase beta subunit was assessed by mutagenic analysis in the Escherichia coli enzyme. Mutations gave variable effects on F1 sector activity, stability, and membrane binding to the F0 sector. Upon solubilization, F1 sectors of the betaD302E and be...

journal_title：Archives of biochemistry and biophysics

authors： Omote H,Tainaka K,Fujie K,Iwamoto-Kihara A,Wada Y,Futai M

更新日期：1998-10-15 00:00:00

abstract：:A class II Zn(2+)-dependent fructose-1,6-bisphosphate (FBP)- aldolase was purified from an overproducer strain of Escherichia coli and characterized by standard biochemical techniques and 13C NMR spectroscopy. The principal finding of these studies was identification, by 13C NMR spectroscopy, of an enzyme-bound reacti...

journal_title：Archives of biochemistry and biophysics

authors： Szwergold BS,Ugurbil K,Brown TR

更新日期：1995-02-20 00:00:00

abstract：:The protein sequence encoded by a creatine transporter cDNA cloned from a human heart library was identical to that cloned from a human kidney library (Nash et al., Receptors Channels 2, 165-174, 1994), except that at position 285 the former contained an Ala residue and the latter contained a Pro residue. Expression o...

journal_title：Archives of biochemistry and biophysics

authors： Dai W,Vinnakota S,Qian X,Kunze DL,Sarkar HK

更新日期：1999-01-01 00:00:00

abstract：:Recent X-ray crystal structures of human cytochrome P450 2B6 and rabbit cytochrome P450 2B4 in complex with amlodipine showed two bound ligand molecules, one in the active site and one in the substrate access channel. Based on the X-ray crystal structures, we investigated the interactions of P450 2B4 and 2B6 with amlo...

journal_title：Archives of biochemistry and biophysics

authors： Jang HH,Davydov DR,Lee GY,Yun CH,Halpert JR

更新日期：2014-03-01 00:00:00