Glycolytic oscillations and intracellular K+ concentration are strongly coupled in the yeast Saccharomyces cerevisiae.

abstract：:Previous studies with methyl ethyl ketone peroxide (MEKP), a radical generator, showed depletion of plasma vitamin E and liver glutathione (GSH) levels prior to a decrease of liver vitamin E levels. Since hepatic pools of this vitamin may serve to maintain circulating levels of vitamin E under conditions of oxidative ...

journal_title：Archives of biochemistry and biophysics

authors： Warren DL,Brown MK,Reed DJ

更新日期：1991-08-01 00:00:00

abstract：:Nonspecific lipid transfer protein (sterol carrier protein2) has previously been proposed to function as (i) a catalyst for intracellular movement of newly synthesized phospholipid, (ii) a cofactor in the biosynthesis and metabolism of cholesterol, and (iii) a cofactor in the feedback inhibition of cholesterol synthes...

journal_title：Archives of biochemistry and biophysics

authors： Crain RC,Clark RW

更新日期：1985-08-15 00:00:00

abstract：:The acute effect of ethanol on hepatic protein synthesis is a rather controversial issue. In view of the conflicting reports on this subject, the effect of ethanol on protein labeling from L-[3H]valine in isolated liver cells was studied under a variety of experimental conditions. When tracer doses of the isotope were...

journal_title：Archives of biochemistry and biophysics

authors： Girbes T,Susin A,Ayuso MS,Parrilla R

更新日期：1983-10-01 00:00:00

abstract：:The interaction of reducing sugars with proteins leads to the formation of advanced glycation end products (AGE) and reactive oxidative species (ROS). ROS peroxidise free or membrane included unsaturated fatty acids, leading to generate reactive aldehydes as advanced lipid peroxidation end products (ALE). Aldehydes fr...

journal_title：Archives of biochemistry and biophysics

authors： Nooshi-Nedamani S,Habibi-Rezaei M,Farzadfard A,Moosavi-Movahedi AA

更新日期：2019-06-15 00:00:00

abstract：:Prostacyclin (PGI(2)) synthase is a heme-thiolate (P450) protein which reacts with low levels of peroxynitrite (PN) under tyrosine nitration and inactivation. Studying heme proteins as models, we have found the heme-thiolate protein NADH-NO reductase (P450(NOR)) to be highly efficient in decomposing PN under concomita...

journal_title：Archives of biochemistry and biophysics

authors： Zou MH,Daiber A,Peterson JA,Shoun H,Ullrich V

更新日期：2000-04-01 00:00:00

abstract：:The effects of the addition of hypolipidemic drugs and 1-acylglycerolipids on the metabolism of oleate in plants have been studied in vivo and in vitro. Using aged potato slices with [14C]oleate as a precursor, it was found that these drugs markedly inhibited both the incorporation into complex lipids and the desatura...

journal_title：Archives of biochemistry and biophysics

authors： Sánchez J,Stumpf PK

更新日期：1984-01-01 00:00:00

abstract：:The flavoprotein monoamine oxidase B (MAO B) from bovine liver, as isolated, has an unusual additional absorption band at 412 nm, which is similar to the absorption of its anionic flavin semiquinone form, (Fl.-), and other typical (Fl.-) flavoproteins. Denaturation of the enzyme results in the elimination of this anom...

journal_title：Archives of biochemistry and biophysics

authors： Yue KT,Bhattacharyya AK,Zhelyaskov VR,Edmondson DE

更新日期：1993-01-01 00:00:00

abstract：:The various interactions of rat liver dihydrofolate reductase with two unconjugated 7,8-dihydropteridines, 7,8-dihydrobiopterin and 6-methyl-7,8-dihydropteridine, have been compared with those of 7,8-dihydrofolate and folate. Of particular interest was the reactivity demonstrated by 7,8-dihydrobiopterin because of the...

journal_title：Archives of biochemistry and biophysics

authors： Webber S,Whiteley JM

更新日期：1985-02-01 00:00:00

abstract：:A cyanide-insensitive superoxide dismutase was purified to electrophoretic homogeneity from leaves of the eucaryote, Ginkgo biloba L. A molecular mass of 47,000 was determined for the enzyme, which consisted of two subunits of equal size. The enzyme preparation contained two isoenzymes with isoelectric points of 5.25 ...

journal_title：Archives of biochemistry and biophysics

authors： Duke MV,Salin ML

更新日期：1985-11-15 00:00:00

abstract：:Nanoceria were synthesized by discharging plasma at 800 V with a frequency of 30 kHz for 0-25 min using a pulsed unipolar power supply into solutions containing 1 or 2 mM of Ce(NO3)2. UV-Vis spectroscopy showed a characteristic absorbance maxima at 304-320 nm for the nanoceria with increase in the intensity of the pea...

journal_title：Archives of biochemistry and biophysics

authors： Davoodbasha M,Park BR,Rhee WJ,Lee SY,Kim JW

更新日期：2018-05-01 00:00:00

abstract：:Ferredoxin reductase (Fd-reductase) supplies reducing equivalents obtained from NADPH to mitochondrial cytochrome P450 enzymes via the small iron-sulfur protein ferredoxin. Two cDNAs (differing by the presence or absence of an 18-bp insert in the coding region) for the human Fd-reductase were subcloned into a newly co...

journal_title：Archives of biochemistry and biophysics

authors： Brandt ME,Vickery LE

更新日期：1992-05-01 00:00:00

abstract：:In order to characterize cellular retinoic acid-binding protein (CRABP) from chick embryos, we resolved the [3H]RA-binding activity of embryo extracts by Mono Q anion-exchange chromatography. Fractions containing [3H]RA-binding proteins eluted in two peaks at 7 and 10 min with the latter showing 10-fold higher activit...

journal_title：Archives of biochemistry and biophysics

authors： Venepally P,Reddy LG,Sani BP

更新日期：1996-12-15 00:00:00

abstract：:Studies were carried out at pH 7.0 and gamma/2 0.15 before addition of CaCl2 or EDTA. Clotting time, tau, at 3.03 microM fibrinogen and 0.91 u/ml thrombin was determined for equilibrium systems. With added Ca2+, tau decreases, from tau 0 at 0 added Ca2+ (mean, 29.7 +/- 3 s), by approximately 3 s at 5 mM added Ca2+. Wi...

journal_title：Archives of biochemistry and biophysics

authors： Perizzolo KE,Sullivan S,Waugh DF

更新日期：1985-03-01 00:00:00

abstract：:The key technique in proteome analysis is high-resolution two-dimensional (2D) electrophoretic separation of proteins from biological samples. This method combines isoelectric focusing (IEF) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Derivatization of protein carbonyls with 2, 4-dinitrop...

journal_title：Archives of biochemistry and biophysics

authors： Reinheckel T,Körn S,Möhring S,Augustin W,Halangk W,Schild L

更新日期：2000-04-01 00:00:00

abstract：:Human leukemic T-lymphocytes undergo extensive and rapid apoptosis in the presence of L1AD3, a small cyclic peptide derivative of cobra cardiotoxin. The first step in this process involves its binding to membranes of susceptible cells. By the use of a biotin "handle" synthetically incorporated at the N-terminus of L1A...

journal_title：Archives of biochemistry and biophysics

authors： Smith CA,Hinman CL

更新日期：2004-12-01 00:00:00

abstract：:CYP119 from Sulfolobus solfataricus is the first well-characterized thermophilic cytochrome P450 enzyme. The endogenous substrate for this enzyme is not known but it hydroxylates lauric acid in a reaction supported by surrogate mesophilic electron donors. However, reconstitution of a high-temperature catalytic system ...

journal_title：Archives of biochemistry and biophysics

authors： Puchkaev AV,Ortiz de Montellano PR

更新日期：2005-02-01 00:00:00

abstract：:The vesicle-to-micelle structural transitions that occurred in the interaction of sodium dodecyl sulfate with phosphatidylcholine vesicles were studied at the equilibrium by means of dynamic light scattering (at different scattering angles) and freeze-fracture electron microscopy techniques. The incorporation of surfa...

journal_title：Archives of biochemistry and biophysics

authors： López O,Cócera M,Wehrli E,Parra JL,de la Maza A

更新日期：1999-07-15 00:00:00

abstract：:In vitro phosphorylation of purified spectrin dimer was studied in the presence of Ca2+-calmodulin (CaM). CaM inhibited autophosphorylation of the beta subunit of spectrin. The inhibitory effect (65% at a 32-fold molar excess) appeared to be due to a weak interaction of CaM with spectrin. CaM was similarly effective i...

journal_title：Archives of biochemistry and biophysics

authors： Maretzki D,Lutz HU

更新日期：1986-04-01 00:00:00

abstract：:Transport of the antifolate cancer drug methotrexate was studied in vesicles isolated from the basolateral membrane of rat liver. Transport of methotrexate by basolateral membrane vesicles (BLMVs) was mostly via uptake into an osmotically active intravesicular space, with some binding (approximately 9%), as shown by i...

journal_title：Archives of biochemistry and biophysics

authors： Horne DW,Reed KA

更新日期：1992-10-01 00:00:00

abstract：:Phosphatidylserine (PS) is predominantly confined to the inner leaflet of plasma membrane in cells, but it is externalized on the cell surface during apoptosis. This externalized PS is required for effective phagocytosis of apoptotic cells by macrophages. Because PS trans-bilayer asymmetry is not absolute in different...

journal_title：Archives of biochemistry and biophysics

authors： Borisenko GG,Matsura T,Liu SX,Tyurin VA,Jianfei J,Serinkan FB,Kagan VE

更新日期：2003-05-01 00:00:00

abstract：:The human folate receptor (hFR) is a plasma membrane protein that is anchored to the membrane via a glycosylphosphatidylinositol (GPI) tail in some cell types. The KB hFR cDNA sequence predicts a hydrophobic, alpha-helical 31-residue carboxyl terminus that is thought to be the signal for cleavage and attachment of the...

journal_title：Archives of biochemistry and biophysics

authors： Chung KN,Roberts S,Kim CH,Kirassova M,Trepel J,Elwood PC

更新日期：1995-09-10 00:00:00

abstract：:Earlier studies have shown that the Fur (ferric uptake regulation) protein acts as an anaerobic/aerobic repressor of MnSOD (sodA) expression. We found that the aerobic expression of sodA::lacZ fusion in a Fur- background to be threefold higher than in a Fur+ background. This effect of fur mutation was not seen in a st...

journal_title：Archives of biochemistry and biophysics

authors： Schrum LW,Hassan HM

更新日期：1994-03-01 00:00:00

abstract：:Pyruvate carboxylase deficiency was previously reported to be the biochemical lesion in a yeast mutant, designated pyc, which cannot utilize ethanol, acetate, pyruvate, aspartate, or oxaloacetate as the sole carbon source [C. Wills and T. Melham (1985) Arch. Biochem. Biophys. 236, 782-791; C. Wills et al. (1986) Arch....

journal_title：Archives of biochemistry and biophysics

authors： Lim F,Rohde M,Morris CP,Wallace JC

更新日期：1987-10-01 00:00:00

abstract：:The association of myelin basic protein charge isomers with the lipid part of the myelin membrane was investigated at the microscopic (molecular) level in a model membrane system, using optical waveguide lightmode spectrometry to determine with high precision the kinetics of association and dissociation to planar phos...

journal_title：Archives of biochemistry and biophysics

authors： Shanshiashvili LV,Suknidze NCh,Machaidze GG,Mikeladze DG,Ramsden JJ

更新日期：2003-11-15 00:00:00

abstract：:L-Tryptophan is an essential amino acid for mammals. It is utilized not only for protein synthesis but also for the biosynthesis of serotonin and melatonin by the serotonin pathway as well as nicotinamide adenine dinucleotide by the kynurenine pathway. Although the kynurenine pathway is responsible for the catabolism ...

journal_title：Archives of biochemistry and biophysics

authors： Geng J,Liu A

更新日期：2014-02-15 00:00:00

abstract：:Atroxase, isolated from the venom of Crotalus atrox (western diamondback rattlesnake), is a nonhemorrhagic protease which has fibrinolytic activity in vivo. The primary structure of atroxase was deduced from the cDNA encoding the atroxase protein. The venom glands of Crotalus atrox were used to prepare a cDNA library....

journal_title：Archives of biochemistry and biophysics

authors： Baker BJ,Wongvibulsin S,Nyborg J,Tu AT

更新日期：1995-03-10 00:00:00

abstract：:A series of esters of adenosine 5'-monophosphate with ethyl, propyl, or hexyl moieties substituted at the omega-position with chlorine or bromine were prepared. The compounds were competitive inhibitors of horse liver alcohol dehydrogenase with respect to coenzyme, NAD+, and had inhibition (dissociation) constants in ...

journal_title：Archives of biochemistry and biophysics

authors： Fries RW,Bohlken DP,Murch BP,Leidal KG,Plapp BV

更新日期：1983-08-01 00:00:00

abstract：:The mechanism by which phenylalanine ammonia-lyase (PAL, EC 4.3.1.5) catalyzes the reversible elimination of ammonia from phenylalanine yielding (E)-cinnamic acid has gained much attention in the recent years. Dehydroalanine is essential for the catalysis. It was assumed that this prostetic group acts as the electroph...

journal_title：Archives of biochemistry and biophysics

authors： Lewandowicz A,Jemielity J,Kańska M,Zoń J,Paneth P

更新日期：1999-10-15 00:00:00

abstract：:The essential Zn(II) in bovine liver dihydropyrimidine amidohydrolase (DHPase) was removed by incubation with 2,6-dipicolinic acid and replaced with Mn(II). Electron paramagnetic resonance studies of Mn(II) binding show that there are four binding sites per tetramer, and the dissociation constant at pH 7.5 is 13.5 mic...

journal_title：Archives of biochemistry and biophysics

authors： Lee MH,Pettigrew DW,Sander EG,Nowak T

更新日期：1987-12-01 00:00:00

abstract：:Arachidonate 12-lipoxygenase (12-LO) from porcine leukocytes was expressed in insect cells using a baculovirus expression vector. The recombinant 12-LO was expressed as an N-terminal fusion protein with a 31-amino acid polypeptide carrying a six-histidine tag and an enterokinase cleavage site. Maximal intracellular en...

journal_title：Archives of biochemistry and biophysics

authors： Reddy RG,Yoshimoto T,Yamamoto S,Funk CD,Marnett LJ

更新日期：1994-07-01 00:00:00