The process of folding proteins into membranes: challenges and progress.

abstract：:Electron spin resonance (ESR) spectroscopy and spin label techniques have been used to study the effects of fumonisin B1 (FB1) and hydrolyzed fumonisin backbone (AP1) on the structural and dynamic properties of phosphatidylcholine membranes at the molecular level. Multilamellar liposomes consisting of dimyristoylphosp...

journal_title：Archives of biochemistry and biophysics

authors： Yin JJ,Smith MJ,Eppley RM,Troy AL,Page SW,Sphon JA

更新日期：1996-11-01 00:00:00

abstract：:Radiotherapy plays a limited role in the treatment of hepatocellular carcinoma (HCC) due to the development of resistance. Therefore, further investigation of underlying mechanisms involved in HCC radioresistance is warranted. Increasing evidence shows that long non-coding RNAs (linc-RNAs) are involved in the patholog...

journal_title：Archives of biochemistry and biophysics

authors： Chen Y,Shen Z,Zhi Y,Zhou H,Zhang K,Wang T,Feng B,Chen Y,Song H,Wang R,Chu X

更新日期：2018-05-01 00:00:00

abstract：:Horseradish peroxidase A1 thermal stability was studied by steady-state fluorescence, circular dichroism and differential scanning calorimetry at pH values of 4, 7 and 10. Changes in the intrinsic protein probes, tryptophan fluorescence, secondary structure, and heme group environment are not coincident. The T(m) valu...

journal_title：Archives of biochemistry and biophysics

authors： Carvalho AS,Melo EP,Ferreira BS,Neves-Petersen MT,Petersen SB,Aires-Barros MR

更新日期：2003-07-15 00:00:00

abstract：:The localization, expression, and transport of two lysosomal integral membrane glycoproteins of human cells, hLAMP-1 and hLAMP-2, have been studied in mucolipidosis II (I-cell disease) fibroblasts. These cells are deficient in N-acetylglucosaminylphosphotransferase, one of the enzymes required for addition of the mann...

journal_title：Archives of biochemistry and biophysics

authors： Sandoval IV,Chen JW,Yuan L,August JT

更新日期：1989-05-15 00:00:00

abstract：:The type I phosphatase associated with glycogen, PP1G, plays an important role in glycogen metabolism. PP1G is targeted to glycogen by the R(GL) subunit, which regulates the function of the enzyme. We report the cloning and characterization of the gene as well as the pattern of expression of the R(GL) subunit from mou...

journal_title：Archives of biochemistry and biophysics

authors： Lannér C,Suzuki Y,Bi C,Zhang H,Cooper LD,Bowker-Kinley MM,DePaoli-Roach AA

更新日期：2001-04-01 00:00:00

abstract：:Plant 3-hydroxy-3-methylglutaryl-CoA reductase(HMGR; EC 1.1.1.34) and sucrose-phosphate synthase (SPS; EC 2.4.1.14) and synthetic peptides designed from the known phosphorylation sites of plant HMGR (SAMS*: KSHMKYNRSTKDVK), rat acetyl-CoA carboxylase (SAMS: HMRSAMSGLHLVKRR), spinach SPS (SP2: GRRJRRISSVEJJDKK), and sp...

journal_title：Archives of biochemistry and biophysics

authors： Toroser D,Huber SC

更新日期：1998-07-15 00:00:00

abstract：:Fibroblast growth factor (FGF) 1 is known to be released in response to cellular stress conditions through formation of a multi-protein complex with synaptotagmin 1 and S100A13. In this study, we characterized the denaturant-induced unfolding of synaptotagmin 1, C2A domain in a residue-specific manner by NMR spectrosc...

journal_title：Archives of biochemistry and biophysics

authors： Anbazhagan V,Wang HM,Lu CS,Yu C

更新日期：2009-10-15 00:00:00

abstract：:A new fluorescent ATP analog, adenosine-5'-triphosphoro-gamma-1-(5-sulfonic acid)naphthyl ethylamidate (gamma-1,5-EDANS)ATP, containing the fluorophore N-(aminoethyl)-5-naphthylamine-1-sulfonic acid attached via a gamma-phosphoamidate bond was synthesized in good yield. It has absorption maxima at 255 and 344 nm and a...

journal_title：Archives of biochemistry and biophysics

authors： Wu FY,Abdulwajid AW,Solaiman D

更新日期：1986-05-01 00:00:00

abstract：:Human seminal fluid was recently found to contain 18,19-dehydroprostaglandins E1 and E2 (E. H. Oliw, H. Sprecher, and M. Hamberg, (1986) J. Biol. Chem. 261, 2675-2683). In the present study, the cis and trans isomers of 18,19-dehydroprostaglandins E1 and E2 were prepared by incubation of microsomes of ram vesicular gl...

journal_title：Archives of biochemistry and biophysics

authors： Oliw EH,Benthin G,Hamberg M

更新日期：1987-10-01 00:00:00

abstract：:Proliferation and migratory behavior of L929 murine fibroblasts were shown to be modified in the presence of a cytosolic extract of Phormidium sp. (Cyanobacteria). The addition of Phormidium extract to the growth medium (Dulbecco's modified Eagle's medium) supplemented with 0.5% newborn calf serum increased cell proli...

journal_title：Archives of biochemistry and biophysics

authors： Hodara ML,Bré MH,Bouzinba H,Pouphile M,Lefort-Tran M

更新日期：1991-04-01 00:00:00

abstract：:The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that mediates the toxicity of dioxin and other xenobiotics. In the absence of exogenous ligand, AhR is cytosolic. We investigated how AhR is retained in the cytosol and how dioxin induces AhR to move to the nucleus. Disruption of nuclear ex...

journal_title：Archives of biochemistry and biophysics

authors： Richter CA,Tillitt DE,Hannink M

更新日期：2001-05-15 00:00:00

abstract：:Raspberries are a rich source of ellagitannins and anthocyanins. The aim of this work was to investigate whether raspberry consumption can improve vascular function and to understand which phenolic metabolites may be responsible for the effects. A 3 arm double-blind randomized controlled crossover human intervention t...

journal_title：Archives of biochemistry and biophysics

authors： Istas G,Feliciano RP,Weber T,Garcia-Villalba R,Tomas-Barberan F,Heiss C,Rodriguez-Mateos A

更新日期：2018-08-01 00:00:00

abstract：:Reports suggest that microRNAs have implications in the development of several diseases including cancer. It is therefore believed that miRs may act as therapeutic targets for cancer treatment. The treatment of ovarian cancer is mainly obstructed by lack of biomarkers and efficient drug targets. Against this backdrop,...

journal_title：Archives of biochemistry and biophysics

authors： Si L,Jia Y,Lin R,Jian W,Yu Q,Yang S

更新日期：2019-06-15 00:00:00

abstract：:I had the fortune to be introduced to Helmut Sies during the mid 1980s, while working as a post-doctoral scientist at the University of California, Berkeley. At that time, Helmut was a frequent visitor of the Bruce Ames' laboratory and a leading authority in antioxidants and oxidative stress. His concepts, ideas and w...

journal_title：Archives of biochemistry and biophysics

authors： Stocker R

更新日期：2016-04-01 00:00:00

abstract：:Platelet-activating factor (PAF) plays an important role in a number of biological processes ranging from inflammation to reproductive biology. We have reported that the enzyme that inactivates this potent autacoid, PAF-acetylhydrolase (PAF-AH), is decreased in maternal plasma during the latter stages of pregnancy. Th...

journal_title：Archives of biochemistry and biophysics

authors： Narahara H,Frenkel RA,Johnston JM

更新日期：1993-03-01 00:00:00

abstract：:The secondary structures of staphylococcal nuclease (SNase) have been assigned and semiquantitatively estimated from the deconvoluted Fourier transformed infrared (FTIR) spectrum. The changes in the secondary structures accompanying unfolding and refolding of SNase during reversible thermal denaturation up to 70 degre...

journal_title：Archives of biochemistry and biophysics

authors： Xie L,Jing GZ,Zhou JM

更新日期：1996-04-01 00:00:00

abstract：:The ionophore properties of cationomycin and monensin were studied on human erythrocytes by measuring Na+ influx by 23Na NMR and concomitant K+ efflux by potentiometry in the presence of increasing amounts of serum. Both ion currents (Na+ or K+) decreased linearly with the reciprocal of serum amount. The serum effects...

journal_title：Archives of biochemistry and biophysics

authors： Gibot S,Jeminet G,Juillard J,Gumila C,Ancelin ML,Vial H,Delort AM

更新日期：1999-03-15 00:00:00

abstract：:Electron spin lattice relaxation times (T1) and the phase memory times (Tm) were obtained for the synthetic melanin system from 3-hydroxytyrosine (dopa) by means of electron spin echo spectroscopy at 77 degrees K. Saturation behavior of the ESR spectra of melanins in melanin-containing tissue and of the synthetic mela...

journal_title：Archives of biochemistry and biophysics

authors： Okazaki M,Kuwata K,Miki Y,Shiga S,Shiga T

更新日期：1985-10-01 00:00:00

abstract：:The zinc-binding motif (HELLGH) of dipeptidyl peptidase III (DPP III) is different from the common zinc-binding motif (HExxH) of metallopeptidases. To clarify the importance of the zinc-binding motif part of DPP III for enzymatic activity, we measured the recovery of the enzyme activity of apo-Leu(453)-deleted dipepti...

journal_title：Archives of biochemistry and biophysics

authors： Hirose J,Kamigakiuchi H,Iwamoto H,Fujii H,Nakai M,Takenaka M,Kataoka R,Sugahara M,Yamamoto S,Fukasawa KM

更新日期：2004-11-01 00:00:00

abstract：:The maximal amounts of Ca2+ being accumulated (delta Ca2+max) and H+ emitted (delta H+max) by Ca2+-loading mitochondria, with succinate (+rotenone) as respiratory substrate, were evaluated. delta Ca2+max was increased by providing either citrate or ATP to a Pi- and Mg2+-free medium. With citrate, delta H+max was only ...

journal_title：Archives of biochemistry and biophysics

authors： Guidoux R

更新日期：1985-12-01 00:00:00

abstract：:Inactivation of the von Hippel-Lindau (VHL) tumor suppressor is associated with renal carcinoma, hemangioblastoma and pheochromocytoma. The VHL protein is a component of a ubiquitin ligase complex that ubiquitinates and degrades hypoxia inducible factor-α (HIF-α). Degradation of HIF-α by VHL is proposed to suppress tu...

journal_title：Archives of biochemistry and biophysics

authors： Lai Y,Song M,Hakala K,Weintraub ST,Shiio Y

更新日期：2012-02-15 00:00:00

abstract：:3-Methyl-substituted fatty acids are first oxidatively decarboxylated (alpha-oxidation) before they are degraded further via beta-oxidation. We synthesized [1-14C]phytanic and 3-[1-14C]methylmargaric acids in order to study their alpha-oxidation in isolated rat hepatocytes, rat liver homogenates and subcellular fracti...

journal_title：Archives of biochemistry and biophysics

authors： Huang S,Van Veldhoven PP,Vanhoutte F,Parmentier G,Eyssen HJ,Mannaerts GP

更新日期：1992-07-01 00:00:00

abstract：:Chlorite dismutase-like proteins are structurally closely related to functional chlorite dismutases which are heme b-dependent oxidoreductases capable of reducing chlorite to chloride with simultaneous production of dioxygen. Chlorite dismutase-like proteins are incapable of performing this reaction and their biologic...

journal_title：Archives of biochemistry and biophysics

authors： Hofbauer S,Hagmüller A,Schaffner I,Mlynek G,Krutzler M,Stadlmayr G,Pirker KF,Obinger C,Daims H,Djinović-Carugo K,Furtmüller PG

更新日期：2015-05-15 00:00:00

abstract：:Adriamycin (AdM) and related anthracyclines are potent antineoplastic agents, the clinical utility of which is limited by severe cardiotoxicity. Aglycone derivatives of AdM have recently been reported to trigger the release of Ca2+ from isolated, preloaded rat heart mitochondria and to modify mitochondrial sulfhydryl ...

journal_title：Archives of biochemistry and biophysics

authors： Sokolove PM

更新日期：1991-02-01 00:00:00

abstract：:An intermediate state of lentil lectin was characterized at pH 1 having low content of secondary as well as tertiary structure. Far- and near-UV CD spectroscopy showed loss of structure when pH was lowered from 7 to 0.8 but the structure loss was less than that of the protein in presence of 6M GndHCl. Intrinsic trypto...

journal_title：Archives of biochemistry and biophysics

authors： Naseem F,Khan RH

更新日期：2004-11-15 00:00:00

abstract：:A soluble monoterpene primary alcohol:NADP+ oxidoreductase has been purified to apparent homogeneity from leaves of the catmint, Nepeta racemosa. The purified enzyme consisted of two polypeptides, with molecular masses of 42,000 and 40,000 Da, and contained zinc ions. A number of monoterpene alcohols (geraniol, nerol,...

journal_title：Archives of biochemistry and biophysics

authors： Hallahan DL,West JM,Wallsgrove RM,Smiley DW,Dawson GW,Pickett JA,Hamilton JG

更新日期：1995-04-01 00:00:00

abstract：:Mutagenic substitution of the invariant D42 and D169 residues in phosphoribulokinase (PRK) with amino acids that contain neutral side chains (e.g., alanine or asparagine) results in large decreases in catalytic efficiency (10(5)- and 10(4)-fold for replacement of D42 and D169, respectively). To further evaluate the im...

journal_title：Archives of biochemistry and biophysics

authors： Runquist JA,Miziorko HM

更新日期：2002-09-15 00:00:00

abstract：:Fibronectin heterogeneity is, in part, the result of post-translational modifications. In these experiments, cartilage fibronectins were purified by anion exchange chromatography, followed by gelatin affinity chromatography or immunoprecipitation, and, finally, sodium dodecyl sulfate--polyacrylamide gel electrophoresi...

journal_title：Archives of biochemistry and biophysics

authors： Burton-Wurster N,Lust G

更新日期：1993-11-01 00:00:00

abstract：:Glaucoma is the leading cause of irreversible blindness in the world and trabeculectomy remains still the most commonly performed filtration surgery. Failure of trabeculectomy is due to the formation of scarring, which is associated with the increased fibroblast proliferation, activation, and collagen deposition at th...

journal_title：Archives of biochemistry and biophysics

authors： Wang L,Guo D,Wang Z,Yin X,Wei H,Hu W,Chen R,Chen C

更新日期：2020-04-15 00:00:00

abstract：:Expression of several forms of cytochrome P450 2B and of P450 4B1 in rabbit kidney was investigated by cloning from cDNA libraries constructed with renal mRNA from animals treated with phenobarbital. Isolation and sequencing of several cDNAs demonstrated that: (i) cytochrome P450 2B-B0 can be found in rabbit kidney al...

journal_title：Archives of biochemistry and biophysics

authors： Ryan R,Grimm SW,Kedzie KM,Halpert JR,Philpot RM

更新日期：1993-08-01 00:00:00