Abstract:
:Glaucoma is the leading cause of irreversible blindness in the world and trabeculectomy remains still the most commonly performed filtration surgery. Failure of trabeculectomy is due to the formation of scarring, which is associated with the increased fibroblast proliferation, activation, and collagen deposition at the site of the drainage channel with subconjunctival fibrosis. Our previous study has revealed that zinc oxide (ZnO) nanoparticles could efficiently decrease the expressions of TGF-β1 and inhibit fibroblast-mediated collagen lattice contraction. However, the mechanism underlying ZnO nanoparticle-induced fibroblast apoptosis is still unclear. In the present study, we investigated the effect of ZnO nanoparticles on the reactive oxygen species (ROS) and mitochondrial membrane potential (Δψm) in human Tenon fibroblasts (HTFs). Moreover, we also explored the influence of ZnO nanoparticles on the expression of Caspase-3, Caspase-9, apoptotic protease-activating factor-1 (Apaf-1), fibroblast-specific protein-1 (FSP-1), collagen III, and E-cadherin. The results indicated that ZnO nanoparticles markedly inhibit HTFs viability and decrease the Δψm in a concentration-dependent pattern. Exposure of HTFs to ZnO nanoparticles could also induce the elevated Caspase-3, Caspase-9, and Apaf-1 expression, decrease the levels of FSP-1, collagen III, and E-cadherin expression, leading to HTFs apoptosis. Our results suggested that elevated ROS and activated Caspase signaling play a fundamental role in ZnO nanoparticle-induced HTFs apoptosis.
journal_name
Arch Biochem Biophysjournal_title
Archives of biochemistry and biophysicsauthors
Wang L,Guo D,Wang Z,Yin X,Wei H,Hu W,Chen R,Chen Cdoi
10.1016/j.abb.2020.108324subject
Has Abstractpub_date
2020-04-15 00:00:00pages
108324eissn
0003-9861issn
1096-0384pii
S0003-9861(19)31108-7journal_volume
683pub_type
杂志文章abstract::Cellular distribution in rat livers of the mannan-binding protein, a liver lectin specific for mannose and N-acetylglucosamine was investigated. Estimation of mannan binding activity of isolated cells demonstrated that more than 99% of the mannan binding activity recovered from whole livers was derived from hepatocyte...
journal_title:Archives of biochemistry and biophysics
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doi:10.1016/0003-9861(83)90552-0
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abstract::Vitamin D was discovered as an anti-rachitic agent, but even at present, there is no direct evidence to support the concept that vitamin D directly stimulates osteoblastic bone formation and mineralization. It appears to be paradoxical, but vitamin D functions in the process of osteoclastic bone resorption. In 1952, C...
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pub_type: 杂志文章,评审
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abstract::Functional activities of isolated atria from 4-week diabetic rats and their anoxic tolerance and recovery were compared to those of control rats. The present study showed a two-fold increase in maximum developed contraction force (CF), the rate of force development, and the rate of relaxation in isolated atria from di...
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pub_type: 杂志文章
doi:10.1016/0003-9861(83)90519-2
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pub_type: 杂志文章
doi:10.1006/abbi.1995.1456
更新日期:1995-09-10 00:00:00
abstract::Isoniazid (INH) is one of the oldest drugs for the treatment of tuberculosis (TB) and is of continual clinical and research interest. The aim of the current study is to investigate the ability of INH to induce monocyte differentiation and the underlying signaling pathway involved in this phenomenon using HL-60 cells. ...
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pub_type: 杂志文章
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doi:10.1016/0003-9861(91)90048-n
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journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章,评审
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pub_type: 杂志文章
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journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
doi:10.1006/abbi.1999.1402
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doi:10.1006/abbi.1996.0096
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pub_type: 杂志文章
doi:10.1016/j.abb.2003.08.033
更新日期:2004-01-01 00:00:00
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journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
doi:10.1006/abbi.1994.1407
更新日期:1994-10-01 00:00:00
abstract::Cytosolic extracts from the posterior adductor muscle of the bivalve mollusk Mytilus galloprovincialis contain significant amounts of both cGMP-binding and cGMP-stimulated protein kinase activities. However, photoaffinity labeling with 8-azido-[32P]cGMP revealed only a major cGMP-binding protein with an apparent molec...
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pub_type: 杂志文章
doi:10.1006/abbi.1998.0879
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journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
doi:10.1006/abbi.1997.0537
更新日期:1998-02-15 00:00:00
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doi:10.1016/0003-9861(83)90001-2
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journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
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更新日期:2001-01-01 00:00:00
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journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
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journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
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更新日期:1997-06-15 00:00:00