Characterization and partial amino acid sequence of human plasma glutathione peroxidase.

abstract：:Ischemia/reperfusion injury (IRI) initiates from oxidative stress caused by lack of blood supply and subsequent reperfusion. It is often associated with sterile inflammation, cell death and microvascular dysfunction, which ultimately results in myocardial, cerebral and hepatic IRIs. Reportedly, deregulation of Nrf2 pa...

journal_title：Archives of biochemistry and biophysics

authors： Padmavathi G,Ramkumar KM

更新日期：2021-02-15 00:00:00

abstract：:Diethyl dithiocarbamate (DDC) was used to inhibit the copper- and zinc- containing superoxide dismutase (Cu,ZnSOD) of Escherichia coli in order to expose its physiological function. DDC inhibited the aerobic growth of a sodA sodB mutant much more than it did the growth of a SOD-replete parental strain and this inhibit...

journal_title：Archives of biochemistry and biophysics

authors： Benov L,Fridovich I

更新日期：1996-03-15 00:00:00

abstract：:Vanadate-dependent oxidation of NADH by xanthine oxidase does not require the presence of xanthine and therefore is not due to cooxidation. Addition of NADH or xanthine had no effect on the oxidation of the other substrate. Oxidation of NADH was high at acid pH and oxidation of xanthine was high at alkaline pH. The sp...

journal_title：Archives of biochemistry and biophysics

authors： Khandke L,Gullapalli S,Patole MS,Ramasarma T

更新日期：1986-02-01 00:00:00

abstract：:Short, strong (low barrier) hydrogen bonds occur when the pK values of the atoms sharing the proton are similar. The overall distance is 2.5 A or less, the deuterium fractionation factor is less than 0.5, the proton NMR chemical shift can approach 20 ppm, and deuterium or tritium substitution causes an up-field change...

journal_title：Archives of biochemistry and biophysics

authors： Cleland WW

更新日期：2000-10-01 00:00:00

abstract：:Gene expression in the obligate intracellular bacterium Chlamydia trachomatis ranges from nil in the infectious EB form to high in the dividing RB form. Little is known about the mechanisms of gene regulation in chlamydiae and only a few promoter sequences have been characterized. The purpose of our study was to exami...

journal_title：Archives of biochemistry and biophysics

authors： Shen L,Shi Y,Douglas AL,Hatch TP,O'Connell CM,Chen JM,Zhang YX

更新日期：2000-07-01 00:00:00

abstract：:Binding of ligands to the catalytic center of mammalian triosephosphate isomerase (TPI) induces a conformational change(s) that enhances the specific deamidation of Asn71 at the subunit interface. Deamidation initiates dissociation and degradation of the enzyme in vivo and in vitro. We have utilized circular dichroism...

journal_title：Archives of biochemistry and biophysics

authors： Sun AQ,Yüksel KU,Rao GS,Gracy RW

更新日期：1992-06-01 00:00:00

abstract：:Phosphatidylinositol (PtdIns) and many other phospholipids activated calmodulin (CaM)-dependent phosphatase in the presence or absence of Ca2+, and the stimulation was more pronounced in the presence of Ca2+. In addition, PtdIns modulated the response of phosphatase to CaM: at low and nonstimulatory concentrations (le...

journal_title：Archives of biochemistry and biophysics

authors： Huang SL,Merat D,Cheung WY

更新日期：1989-04-01 00:00:00

abstract：:Prostacyclin (PGI(2)) synthase is a heme-thiolate (P450) protein which reacts with low levels of peroxynitrite (PN) under tyrosine nitration and inactivation. Studying heme proteins as models, we have found the heme-thiolate protein NADH-NO reductase (P450(NOR)) to be highly efficient in decomposing PN under concomita...

journal_title：Archives of biochemistry and biophysics

authors： Zou MH,Daiber A,Peterson JA,Shoun H,Ullrich V

更新日期：2000-04-01 00:00:00

abstract：:We hypothesized that cellular oxygen consumption is abnormal during sepsis as a result of increased oxidative stress and selective mitochondrial damage. In a rat model of sepsis (cecal ligation and puncture), we studied the respiratory characteristics of isolated hepatocytes and liver mitochondria 16 h after onset of ...

journal_title：Archives of biochemistry and biophysics

authors： Kantrow SP,Taylor DE,Carraway MS,Piantadosi CA

更新日期：1997-09-15 00:00:00

abstract：:Human leukemic T-lymphocytes undergo extensive and rapid apoptosis in the presence of L1AD3, a small cyclic peptide derivative of cobra cardiotoxin. The first step in this process involves its binding to membranes of susceptible cells. By the use of a biotin "handle" synthetically incorporated at the N-terminus of L1A...

journal_title：Archives of biochemistry and biophysics

authors： Smith CA,Hinman CL

更新日期：2004-12-01 00:00:00

abstract：:L-Tryptophan is an essential amino acid for mammals. It is utilized not only for protein synthesis but also for the biosynthesis of serotonin and melatonin by the serotonin pathway as well as nicotinamide adenine dinucleotide by the kynurenine pathway. Although the kynurenine pathway is responsible for the catabolism ...

journal_title：Archives of biochemistry and biophysics

authors： Geng J,Liu A

更新日期：2014-02-15 00:00:00

abstract：:The complete amino acid sequence of Rapana thomasiana hemocyanin functional unit RtH2-e was determined by direct sequencing and matrix-assisted laser desorption ionization mass spectrometry of peptides obtained by cleavage with EndoLysC proteinase, chymotrypsin, and trypsin. The single-polypeptide chain of RtH2-e cons...

journal_title：Archives of biochemistry and biophysics

authors： Stoeva S,Idakieva K,Betzel C,Genov N,Voelter W

更新日期：2002-03-15 00:00:00

abstract：:Mutagenic substitution of the invariant D42 and D169 residues in phosphoribulokinase (PRK) with amino acids that contain neutral side chains (e.g., alanine or asparagine) results in large decreases in catalytic efficiency (10(5)- and 10(4)-fold for replacement of D42 and D169, respectively). To further evaluate the im...

journal_title：Archives of biochemistry and biophysics

authors： Runquist JA,Miziorko HM

更新日期：2002-09-15 00:00:00

abstract：:Previous studies showed that vegetative cells of Dictyostelium discoideum make a cysteine proteinase called proteinase-1, which contains multiple residues of GlcNAc-1-P linked directly to peptidyl serines. As a prelude to understanding the function of this novel carbohydrate modification, we purified and extensively c...

journal_title：Archives of biochemistry and biophysics

authors： Mehta DP,Etchison JR,Freeze HH

更新日期：1995-08-01 00:00:00

abstract：:Biophysical evidences suggest that transthyretin (TTR) tetramer dissociation to the monomeric intermediate and subsequent polymerization leads to amyloid fibril formation, which is implicated in the pathogenesis of familial amyloid polyneuropathy (FAP) and senile systemic amyloidosis (SSA). Hence, inhibition of fibril...

journal_title：Archives of biochemistry and biophysics

authors： Pullakhandam R,Srinivas PN,Nair MK,Reddy GB

更新日期：2009-05-15 00:00:00

abstract：:Phorbol esters induce rapid downregulation of PAF receptors on human neutrophils. The molecular mechanism responsible for this observation has not been elucidated. In the present study, treatment of neutrophils with PMA (10(-7) M) for 10 min caused a complete loss of PAF receptors from the cell surface as determined b...

journal_title：Archives of biochemistry and biophysics

authors： Zhou W,Olson MS

更新日期：1994-08-15 00:00:00

abstract：:Fresh human A1 erythrocytes, washed and pretreated in phosphate buffer with or without papain, were incubated at 37 degrees C with blood group-degrading enzymes from the human fecal Ruminococcus torques strain IX-70. The effects were assayed as changes in hemagglutination patterns, and blood group activities of alkali...

journal_title：Archives of biochemistry and biophysics

authors： Falk P,Hoskins LC,Lindstedt R,Svanborg C,Larson G

更新日期：1991-11-01 00:00:00

abstract：:Recombinant CYP4F8 and CYP4F12 metabolize prostaglandin H2 (PGH2) analogs by omega2- and omega3-hydroxylation and arachidonic acid (20:4n-6) by omega3-hydroxylation. CYP4F8 was found to catalyze epoxidation of docosahexaenoic acid (22:6n-3) and docosapentaenoic acid (22:5n-3) and omega3-hydroxylation of 22:5n-6. CYP4F...

journal_title：Archives of biochemistry and biophysics

authors： Stark K,Wongsud B,Burman R,Oliw EH

更新日期：2005-09-15 00:00:00

abstract：:We demonstrate that the efficiency of adenovirus-assisted exogenous Ca(2+) ATPase (SERCA) and reporter (EGFP) gene expression is much higher in primary cultures of myocytes from neonatal rat hearts, than in primary cultures of myocytes from adult rat hearts. In this respect, the neonatal myocytes behave similarly to t...

journal_title：Archives of biochemistry and biophysics

authors： Sumbilla C,Ma H,Seth M,Inesi G

更新日期：2003-07-15 00:00:00

abstract：:The quinoid pigments pthiocol, produced by Mycobacterium tuberculosis, and pyocyanine, produced by Pseudomonas aeruginosa, were examined for their effects on O2.- production in cultured human lung epithelial-like A549 cells. Intracellular O2.- levels were measured using the O2.-sensitive aconitase(s), and rates of O2....

journal_title：Archives of biochemistry and biophysics

authors： Gardner PR

更新日期：1996-09-01 00:00:00

abstract：:Putidaredoxin reductase (PdR), an FAD-containing protein, mediates the transfer of electrons from NADH to putidaredoxin in the cytochrome P-450cam-dependent oxidation of camphor. Using stopped-flow spectrophotometry, reduction of putidaredoxin reductase by NADH (70 microM) at 4 degrees C appeared to be a pseudo-first-...

journal_title：Archives of biochemistry and biophysics

authors： Roome PW,Peterson JA

更新日期：1988-10-01 00:00:00

abstract：:Sendai virus envelopes have been a useful tool in studying the mechanism of membrane-membrane fusion and have served as a vehicle for introducing foreign molecules (e.g., membrane proteins) into recipient cells. Reconstituted Sendai virus envelopes are routinely obtained following solubilization of virus particles wit...

journal_title：Archives of biochemistry and biophysics

authors： Ran S,Nussbaum O,Loyter A,Marikovsky Y,Rivnay B

更新日期：1988-03-01 00:00:00

abstract：:The vitamin D receptor (VDR) is a member of the nuclear receptor superfamily and plays a central role in the biological actions of vitamin D. VDR regulates the expression of numerous genes involved in calcium/phosphate homeostasis, cellular proliferation and differentiation, and immune response, largely in a ligand-de...

journal_title：Archives of biochemistry and biophysics

authors： Wang Y,Zhu J,DeLuca HF

更新日期：2012-07-01 00:00:00

abstract：:The objective of this study was to develop straightforward processes that could be applied to the large-scale production of β-cryptoxanthin in an attempt to facilitate investigation of its biological activity. An oleoresin obtained from crude extracts of marigold flowers (Tagetes erecta) with approximately 24% total l...

journal_title：Archives of biochemistry and biophysics

authors： Khachik F

更新日期：2018-09-01 00:00:00

abstract：:Heparin is thought to regulate the rate of mammalian blood clotting by enhancing the activity of antithrombin, an inhibitor of coagulation enzymes. The present study establishes that this same inhibitor is present in the blood plasma of each of the terrestrial vertebrate groups including mammals, birds, reptiles, and ...

journal_title：Archives of biochemistry and biophysics

authors： Jordan RE

更新日期：1983-12-01 00:00:00

abstract：:Five functional isoforms of a novel plant thiol methyltransferase from the leaves of cabbage (Brassica oleracea L.) were purified to electrophoretic homogeneity. Pooled, partly purified preparations of the enzyme were previously shown to methylate thiol compounds released upon the hydrolysis of glucosinolates. The enz...

journal_title：Archives of biochemistry and biophysics

authors： Attieh J,Sparace SA,Saini HS

更新日期：2000-08-15 00:00:00

abstract：:Serratia rubidaea (ATCC 27614) typifies a substantial number of enteric bacteria which, unlike Escherichia coli, possess a monofunctional species of chorismate mutase (denoted CM-F). CM-F coexists with two additional species of chorismate mutase, each of the latter being one catalytic domain of a bifunctional protein....

journal_title：Archives of biochemistry and biophysics

authors： Xia T,Jensen RA

更新日期：1992-04-01 00:00:00

abstract：:Glucosylsphingosine is a potent inhibitor of lysosomal glucocerebrosidase and the broad-specificity, cytosolic beta-glucosidase of human liver. In the present study, it was demonstrated that the broad-specificity beta-glucosidase was also inhibited by galactosylsphingosine. The inhibition was observed when the enzyme ...

journal_title：Archives of biochemistry and biophysics

authors： LaMarco KL,Glew RH

更新日期：1985-02-01 00:00:00

abstract：:Guanidine hydrochloride-induced denaturation and thermal denaturation of three kinds of tryptophan synthase alpha subunit have been compared by circular dichroism measurements. The three alpha subunits are from Escherichia coli, Salmonella typhimurium, and an interspecies hybrid in which the C-terminal domain comes fr...

journal_title：Archives of biochemistry and biophysics

authors： Yutani K,Sato T,Ogasahara K,Miles EW

更新日期：1984-03-01 00:00:00

abstract：:The modulation of the plasma membrane calcium pump (PMCA) gene expression is not well understood. We now present evidence that PMCA mRNA is induced by various hormones or agonists via multiple second messenger pathways that include protein kinase C-, cAMP-, and Ca(2+)-dependent mechanisms. Among several types of cultu...

journal_title：Archives of biochemistry and biophysics

authors： Kuo TH,Liu BF,Diglio C,Tsang W

更新日期：1993-09-01 00:00:00