Rapid reactions of peroxynitrite with heme-thiolate proteins as the basis for protection of prostacyclin synthase from inactivation by nitration.

abstract：:In order to understand how the Glu54Lys mutation of alpha-tropomyosin affects actomyosin interactions, we labeled SH1 helix of myosin subfragment-1 (S1) and the actin subdomain-1 with fluorescent probes. These proteins were incorporated into ghost muscle fibers and their conformational states were monitored during the...

journal_title：Archives of biochemistry and biophysics

authors： Borovikov YS,Karpicheva OE,Avrova SV,Robinson P,Redwood CS

更新日期：2009-09-01 00:00:00

abstract：:In trout and mammals, the major extrahepatic expression site for CYP3A forms is in the intestine. A cDNA encoding a new CYP3A subfamily member was isolated from rainbow trout intestinal ceca by reverse transcriptase-polymerase chain reaction (RT-PCR), followed by rapid amplification of cDNA ends (RACE)-PCR. In a set o...

journal_title：Archives of biochemistry and biophysics

authors： Lee SJ,Buhler DR

更新日期：2003-04-01 00:00:00

abstract：:Point mutations at position 141 in the murine interleukin-2 (IL-2) sequence have been reported to generate proteins with full antagonist activity on some IL-2-dependent cell lines. To evaluate the potential therapeutic utility of this observation, we have prepared recombinant human IL-2 with a point mutation at the co...

journal_title：Archives of biochemistry and biophysics

authors： Buchli P,Ciardelli T

更新日期：1993-12-01 00:00:00

abstract：:Guanidine hydrochloride-induced denaturation and thermal denaturation of three kinds of tryptophan synthase alpha subunit have been compared by circular dichroism measurements. The three alpha subunits are from Escherichia coli, Salmonella typhimurium, and an interspecies hybrid in which the C-terminal domain comes fr...

journal_title：Archives of biochemistry and biophysics

authors： Yutani K,Sato T,Ogasahara K,Miles EW

更新日期：1984-03-01 00:00:00

abstract：:Restriction fragments isolated from a 17-kb rat genomic DNA clone containing the gene for apolipoprotein (apo) E were radiolabeled and used to screen a rat liver cDNA library. A cDNA clone hybridizing to a 6-kb genomic DNA fragment was isolated and the nucleotide sequence of the cDNA insert determined. The sequence wa...

journal_title：Archives of biochemistry and biophysics

authors： Shen P,Howlett GJ

更新日期：1992-09-01 00:00:00

abstract：:The three-dimensional conformation of a protein provides a wealth of biochemical information and with the advent of cloning techniques that allow the preparation of proteins almost at will, a renewed interest has arisen in the crystallographic determination of protein structures. As in any research technique, however,...

journal_title：Archives of biochemistry and biophysics

authors： Holden HM,Rayment I

更新日期：1991-11-15 00:00:00

abstract：:Several 13C isotope effects of relevance to reactions involving carbamate and carbamoyl phosphate have been determined. The fractionation factor of carbamate relative to aqueous CO2 is 1.011; the equilibrium isotope effect on the reaction catalyzed by carbamate kinase is 0.9983. From these data we can calculate that t...

journal_title：Archives of biochemistry and biophysics

authors： Tipton PA,Cleland WW

更新日期：1988-01-01 00:00:00

abstract：:The subcellular mechanism of alkenyl halide S-conjugate-induced nephrotoxicity was studied in mitochondria isolated from rat kidney cortex in vitro using the cysteine conjugate of hexachloro-1,3-butadiene, i.e., S-pentachlorobutadienyl-L-cysteine (PCBC) as a model substrate. Respiring mitochondria exposed to various c...

journal_title：Archives of biochemistry and biophysics

authors： Wallin A,Jones TW,Vercesi AE,Cotgreave I,Ormstad K,Orrenius S

更新日期：1987-11-01 00:00:00

abstract：:UDP-galactose 4-epimerase from Escherichia coli is a homodimer of molecular weight 39 kDa/subunit having noncovalently bound NAD acting as cofactor. Denaturation by 8 M urea at pH 7.0 causes 85% loss of its secondary structure and dissociation of its constituent molecules. Dilution of the denaturant by buffer at pH 8....

journal_title：Archives of biochemistry and biophysics

authors： Barat B,Bhattacharyya D

更新日期：2001-07-15 00:00:00

abstract：:In bovine retinal pigment epithelium membranes we have found three hydrolases which were active against trans-retinyl palmitate. This was possible by assaying different subcellular fractions as a function of pH in the range 3-9. Detection of these activities has been favored by the use in the enzyme assay of Triton X-...

journal_title：Archives of biochemistry and biophysics

authors： Gueli MC,Nicotra CM,Pintaudi AM,Paganini A,Pandolfo L,De Leo G,Di Bella MA

更新日期：1991-08-01 00:00:00

abstract：:The dicarbonyl compounds, phenylgloxyl and 2,3-butanedione were used to demonstrate the presence of an essential arginine residue in the mechanism of the red beet (Beta vulgaris L.) plasma membrane ATPase. Treatment of the red beet ATPase with either of these reagents resulted in an inhibition of ATP hydrolytic activi...

journal_title：Archives of biochemistry and biophysics

authors： Gildensoph LH,Briskin DP

更新日期：1989-05-15 00:00:00

abstract：:In the present study, the activity of Topoisomerase IIβ (TopoIIβ) is evaluated during peroxide induced double stranded DNA breaks (DSBs) repair in primary neurons. The results showed that the TopoIIβ levels were enhanced during recovery from peroxide mediated damage (PED) along with Ku70, PARP-1, pol beta, and WRN hel...

journal_title：Archives of biochemistry and biophysics

authors： Mandraju R,Chekuri A,Bhaskar C,Duning K,Kremerskothen J,Kondapi AK

更新日期：2011-12-15 00:00:00

abstract：:The roles of p67-deglycosylase (p67-DG) in the regulation of protein synthesis in baculovirus-infected insect cells were studied. Like vaccinia viral infection, baculovirus infection of insect cells also induced the appearance of a p67-DG. However, p67-DG activity could not be detected because these cells do not conta...

journal_title：Archives of biochemistry and biophysics

authors： Saha D,Wu S,Bose A,Chatterjee N,Chakraborty A,Chatterjee M,Gupta NK

更新日期：1997-06-15 00:00:00

abstract：:Four subunits of beta-conglycinin were purified from soybean cultivar CX 635-1-1-1, and were designated alpha, alpha', beta, and beta' in accordance with nomenclature proposed by Thanh and Shibasaki [(1977) Biochim. Biophys. Acta 490, 370-384]. Of these subunits, beta' has not previously been reported or characterized...

journal_title：Archives of biochemistry and biophysics

authors： Coates JB,Medeiros JS,Thanh VH,Nielsen NC

更新日期：1985-11-15 00:00:00

abstract：:Lipoxygenase-induced lipid oxidation contributes to plasma lipoprotein oxidation and may be an underlying pathogenic mechanism of atherogenesis. Since inactivation of the vasorelaxant actions of nitric oxide (.NO) plays a critical role in the impaired function of atherosclerotic vessels and because .NO reacts rapidly ...

journal_title：Archives of biochemistry and biophysics

authors： Rubbo H,Parthasarathy S,Barnes S,Kirk M,Kalyanaraman B,Freeman BA

更新日期：1995-12-01 00:00:00

abstract：:Increasing evidence suggests that long non-coding RNAs (lncRNAs) are implicated with chemoresistance of cancers. However, their functional role and molecular mechanisms in colorectal cancer (CRC) chemoresistance are still largely unclear. In this work, we aimed to investigate the functional role of lncRNA cancer susce...

journal_title：Archives of biochemistry and biophysics

authors： Gao R,Fang C,Xu J,Tan H,Li P,Ma L

更新日期：2019-03-15 00:00:00

abstract：:Binding of ligands to the catalytic center of mammalian triosephosphate isomerase (TPI) induces a conformational change(s) that enhances the specific deamidation of Asn71 at the subunit interface. Deamidation initiates dissociation and degradation of the enzyme in vivo and in vitro. We have utilized circular dichroism...

journal_title：Archives of biochemistry and biophysics

authors： Sun AQ,Yüksel KU,Rao GS,Gracy RW

更新日期：1992-06-01 00:00:00

abstract：:Venom from the common tiger snake, Notechis scutatus scutatus, contains several toxic acidic proteins which promote hypotension and hemorrhage in mice. One of these toxins, HTe, has a phospholipase A2 (PLA2) amino acid sequence. It contains 125 amino acids rather than the 119/120 found in other N. s. scutatus PLA2s, b...

journal_title：Archives of biochemistry and biophysics

authors： Francis B,Coffield JA,Simpson LL,Kaiser II

更新日期：1995-04-20 00:00:00

abstract：:We have isolated and characterized a human liver glutathione S-transferase Ha1 subunit gene. The gene spans approximately 12 kilobases and comprises seven exons separated by six introns. The transcription initiation site has been determined by primer extension analysis. A TATA box is located 26 nucleotides upstream fr...

journal_title：Archives of biochemistry and biophysics

authors： Rozen F,Nguyen T,Pickett CB

更新日期：1992-02-01 00:00:00

abstract：:Two histone acetyltransferases, A and B, have been extracted and partially purified from yeast cells. The purification scheme included ammonium sulfate precipitation, and chromatography on DEAE-Sepharose and Sephadex G-200. The basic properties of both enzymes closely correspond to those of acetyltransferase A and B f...

journal_title：Archives of biochemistry and biophysics

authors： Lopez-Rodas G,Perez-Ortin JE,Tordera V,Salvador ML,Franco L

更新日期：1985-05-15 00:00:00

abstract：:L-ribulokinase is unusual among kinases since it phosphorylates all four 2-ketopentoses with almost the same k(cat) values. The K(m)'s differ, however, being 0.14 mM for L- and 0.39 mM for d-ribulose and 3.4 mM for l- and 16 mM for d-xylulose. In addition, L-arabitol is phosphorylated at C-5 (K(m) 4 mM) and ribitol (a...

journal_title：Archives of biochemistry and biophysics

authors： Lee LV,Gerratana B,Cleland WW

更新日期：2001-12-15 00:00:00

abstract：:Erythrocyte maturation is accompanied by RNA degradation and release of mononucleotides. Pyrimidine 5'-nucleotidase, PN-I, has been purified and characterized. The molecular and enzymatic properties determined for the enzyme shows a 36-kDa and 5.1 pI monomeric protein with no disulfide bridges and no phosphate content...

journal_title：Archives of biochemistry and biophysics

authors： Amici A,Magni G

更新日期：2002-01-15 00:00:00

abstract：:Acidithiobacillus ferrooxidans sulfide:quinone oxidoreductase (SQR) catalyzes the oxidation of sulfide to polysulfide chains or elemental sulfur coupled to quinone reduction via a non-covalent FAD cofactor. We investigated the role of the FAD using kinetics and EPR spectroscopy. The properties of the enzyme were compa...

journal_title：Archives of biochemistry and biophysics

authors： Zhang Y,Weiner JH

更新日期：2014-12-15 00:00:00

abstract：:Three isoenzymes of UDP-glucuronyltransferase (UDPGT) have been separated and purified from liver microsomes of untreated female rats or female rats pretreated with 3-methylcholanthrene. The UDPGT isoenzymes were purified utilizing Chromatofocusing, column isoelectric focusing, and UDP-hexanolamine Sepharose 4B affini...

journal_title：Archives of biochemistry and biophysics

authors： Falany CN,Tephly TR

更新日期：1983-11-01 00:00:00

abstract：:Incubation of either Chlorella nitrate reductase or the recombinant flavin domain of spinach nitrate reductase with reagents specific for modification of cysteine residues, such as N-ethylmaleimide, resulted in a time-dependent inactivation of NADH:ferricyanide reductase activity which could be prevented by incubation...

journal_title：Archives of biochemistry and biophysics

authors： Trimboli AJ,Quinn GB,Smith ET,Barber MJ

更新日期：1996-07-01 00:00:00

abstract：:Activated leukocytes generate the potent oxidants HOCl and HOBr via the formation of H(2)O(2) and the release of peroxidase enzymes (myeloperoxidase, eosinophil peroxidase). HOCl and HOBr are potent microbiocidal agents, but excessive or misplaced production can cause tissue damage and cell lysis. In this study it is ...

journal_title：Archives of biochemistry and biophysics

authors： Hawkins CL,Brown BE,Davies MJ

更新日期：2001-11-15 00:00:00

abstract：:Xenopus laevis nucleoplasmin is a pentameric nuclear chaperone. The relation between the structure and the multifunctional aspects of the molecule has not yet been clearly established. In the course of analysing a C-terminally His-tagged recombinant version of the region equivalent to the trypsin resistant core (r-NP1...

journal_title：Archives of biochemistry and biophysics

authors： Arnan C,Prieto C,Chiva M,Salvany L,Ausió J,Subirana JA,Saperas N

更新日期：2005-05-15 00:00:00

abstract：:Polycation binding to the negatively charged surface of chloroplast thylakoid membranes is known to cause an inhibition of photosystem I activity. It also interferes with the cation-dependent rearrangement of chlorophyll proteins in the thylakoid membrane. It was shown that added anions prevented or reversed the inhib...

journal_title：Archives of biochemistry and biophysics

authors： Richter ML,Homann PH

更新日期：1983-04-01 00:00:00

abstract：:The amino acid sequences of two very different high-potential iron-sulfur protein (HiPIP) isozymes have been determined from the moderately halophilic purple phototrophic bacterium, Rhodospirillum salinarum. Iso-1 HiPIP, which is monomeric and contains 57 amino acid residues, is most similar to the Thiobacillus ferroo...

journal_title：Archives of biochemistry and biophysics

authors： Ambler RP,Daniel M,Meyer TE,Cusanovich MA

更新日期：1999-09-01 00:00:00

abstract：:A number of early experiments suggested a relationship between methyl group metabolism and the exocrine secretion of the pancreas. These included nutritional studies showing that ethionine, the ethyl analog of methionine which inhibits cellular methylation reactions, is a specific pancreatic toxin. Other studies indic...

journal_title：Archives of biochemistry and biophysics

authors： Capdevila A,Decha-Umphai W,Song KH,Borchardt RT,Wagner C

更新日期：1997-09-01 00:00:00