Characterization of the kinetics and electron paramagnetic resonance spectroscopic properties of Acidithiobacillus ferrooxidans sulfide:quinone oxidoreductase (SQR).

abstract：:Alanine:glyoxylate aminotransferase has been reported to be present as the apo enzyme in the peroxisomes and as the holo enzyme in the mitochondria in chick (white leghorn) embryonic liver. However, surprisingly, birds were found to be classified into two groups on the basis of intraperoxisomal forms of liver alanine:...

journal_title：Archives of biochemistry and biophysics

authors： Sakuraba H,Fujiwara S,Noguchi T

更新日期：1991-05-01 00:00:00

abstract：:The enzyme catalase catalyzes the breakdown of hydrogen peroxide into oxygen and water. It is the main regulator of hydrogen peroxide metabolism. Hydrogen peroxide is a highly reactive small molecule formed as a natural byproducts of energy metabolism. Excessive concentrations may cause significant damages to protein,...

journal_title：Archives of biochemistry and biophysics

authors： Góth L,Nagy T

更新日期：2012-09-15 00:00:00

abstract：:(+)-Menthofuran is an undesirable monoterpenoid component of peppermint (Mentha x piperita) essential oil that is derived from the alpha,beta-unsaturated ketone (+)-pulegone. Microsomal preparations, from the oil gland secretory cells of a high (+)-menthofuran-producing chemotype of Mentha pulegium, transform (+)-pule...

journal_title：Archives of biochemistry and biophysics

authors： Bertea CM,Schalk M,Karp F,Maffei M,Croteau R

更新日期：2001-06-15 00:00:00

abstract：:Cancer cells exhibit extreme sensitivity to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) over normal cells, highlighting TRAIL's potential as a novel and effective cancer drug. However, the therapeutic effect of TRAIL is limited due to drug resistance. In the present study, we sought to investigate ...

journal_title：Archives of biochemistry and biophysics

authors： Wu B,Xiong J,Zhou Y,Wu Y,Song Y,Wang N,Chen L,Zhang J

更新日期：2020-10-15 00:00:00

abstract：:Methionine is oxidized to methionine sulfoxide by rat liver and kidney microsomes in an O2- and NADPH-dependent manner. In all microsomal assays, no methionine sulfone was detected. Use of a monoclonal antibody to rat liver cytochrome P-450 reductase, various cytochrome P-450 and peroxidase inhibitors, antioxidants, a...

journal_title：Archives of biochemistry and biophysics

authors： Krause RJ,Ripp SL,Sausen PJ,Overby LH,Philpot RM,Elfarra AA

更新日期：1996-09-01 00:00:00

abstract：:Linamarase (EC 3.2.1.21) was purified from cassava petiole, stem, and root cortex by ammonium sulfate precipitation, column chromatography on Sepharose 6B, and chromatofocusing. The last step resolved the enzyme from each source into three forms with pI values of 4.3, 3.3, and 2.9. Each form was found to be oligomeric...

journal_title：Archives of biochemistry and biophysics

authors： Eksittikul T,Chulavatnatol M

更新日期：1988-10-01 00:00:00

abstract：:The ability for various ligands to modulate the binding of fructose 1,6-bisphosphate (Fru-1,6-P2) with purified rat liver pyruvate kinase was examined. Binding of Fru-1,6-P2 with pyruvate kinase exhibits positive cooperativity, with maximum binding of 4 mol Fru-1,6-P2 per enzyme tetramer. The Hill coefficient (nH), an...

journal_title：Archives of biochemistry and biophysics

authors： Blair JB,Walker RG

更新日期：1984-07-01 00:00:00

abstract：:During the deglycosylation reaction of fish egg polysialoglycoproteins under the conditions of 1 M NaBH4 in 0.1 M NaOH at 37 degrees C for 48 h, a marked loss of the glycine content has been encountered, besides the serine and threonine residues to which the carbohydrate units are linked. The chemical basis behind thi...

journal_title：Archives of biochemistry and biophysics

authors： Shimamura M,Inoue Y,Inoue S

更新日期：1984-08-01 00:00:00

abstract：:A uridinediphosphateglucose:thiohydroximate glucosyltransferase (EC 2.4.1.-) has been purified 3700-fold from Brassica napus L. seedlings. The enzyme catalyzes the formation of desulfoglucosinolates by transfer of glucose from UDP-glucose to thiohydroximates and is believed to be the second to last step involved in gl...

journal_title：Archives of biochemistry and biophysics

authors： Reed DW,Davin L,Jain JC,Deluca V,Nelson L,Underhill EW

更新日期：1993-09-01 00:00:00

abstract：:Glycosaminoglycan (GAG)-protein interactions regulate a myriad of physiologic and pathologic processes, yet an understanding of how these molecules interact is lacking. The role of the pattern and spacing of basic amino acids (arginine (R) and lysine (K)) in heparin binding sites was investigated using peptide analogs...

journal_title：Archives of biochemistry and biophysics

authors： Fromm JR,Hileman RE,Caldwell EE,Weiler JM,Linhardt RJ

更新日期：1997-07-01 00:00:00

abstract：:Mutagenic substitution of the invariant D42 and D169 residues in phosphoribulokinase (PRK) with amino acids that contain neutral side chains (e.g., alanine or asparagine) results in large decreases in catalytic efficiency (10(5)- and 10(4)-fold for replacement of D42 and D169, respectively). To further evaluate the im...

journal_title：Archives of biochemistry and biophysics

authors： Runquist JA,Miziorko HM

更新日期：2002-09-15 00:00:00

abstract：:Restriction fragments isolated from a 17-kb rat genomic DNA clone containing the gene for apolipoprotein (apo) E were radiolabeled and used to screen a rat liver cDNA library. A cDNA clone hybridizing to a 6-kb genomic DNA fragment was isolated and the nucleotide sequence of the cDNA insert determined. The sequence wa...

journal_title：Archives of biochemistry and biophysics

authors： Shen P,Howlett GJ

更新日期：1992-09-01 00:00:00

abstract：:gamma-Synuclein is a small cytoplasmic protein implicated in neurodegenerative diseases and cancer. However, the mechanism of its involvement in diseases is not clear. We studied the role of gamma-synuclein in the regulation of matrix metalloproteinases in retinoblastoma cell culture. Matrix metalloproteinases play im...

journal_title：Archives of biochemistry and biophysics

authors： Surgucheva IG,Sivak JM,Fini ME,Palazzo RE,Surguchov AP

更新日期：2003-02-01 00:00:00

abstract：:Histidine acid phosphatases (HAPs) utilize a nucleophilic histidine residue to catalyze the transfer of a phosphoryl group from phosphomonoesters to water. HAPs function as protein phosphatases and pain suppressors in mammals, are essential for Giardia lamblia excystation, and contribute to virulence of the category A...

journal_title：Archives of biochemistry and biophysics

authors： Dhatwalia R,Singh H,Reilly TJ,Tanner JJ

更新日期：2015-11-01 00:00:00

abstract：:Minoxidil was found to inhibit the proliferation of smooth muscle cells in the proliferating phase, but not in the quiescent phase. Treatment of proliferating or quiescent cells with minoxidil resulted in a dose- and time-dependent stimulation of elastin synthesis specifically. Maximum stimulation (fourfold) occurred ...

journal_title：Archives of biochemistry and biophysics

authors： Hayashi A,Suzuki T,Wachi H,Tajima S,Nishikawa T,Murad S,Pinnell SR

更新日期：1994-11-15 00:00:00

abstract：:In this study, we have analyzed interflavin electron transfer reactions from FAD to FMN in both the full-length inducible nitric oxide synthase (iNOS) and its reductase domain. Comparison is made with the interflavin electron transfer in NADPH-cytochrome P450 reductase (CPR). For the analysis of interflavin electron t...

journal_title：Archives of biochemistry and biophysics

authors： Yamamoto K,Kimura S,Shiro Y,Iyanagi T

更新日期：2005-08-01 00:00:00

abstract：:The diagnostic substrate tetramethylcyclopropane (TMCP) has been reexamined as a substrate with three drug- and xenobiotic-metabolizing cytochrome P450 enzymes, human CYP2E1, CYP3A4 and rat CYP2B1. The major hydroxylation product in all cases was the unrearranged primary alcohol along with smaller amounts of a rearran...

journal_title：Archives of biochemistry and biophysics

authors： Cooper HL,Groves JT

更新日期：2011-03-01 00:00:00

abstract：:Histidinol dehydrogenase (EC 1.1.1.23) activity was determined in several plant species and in cultured plant cell lines. The enzyme was purified from cabbage (Brassica oleracea) to apparent homogeneity. To render complete purification, a new, specific histidinol-Sepharose 4B affinity chromatography was developed. The...

journal_title：Archives of biochemistry and biophysics

authors： Nagai A,Scheidegger A

更新日期：1991-01-01 00:00:00

abstract：:The rates of reactive absorption of ozone by various biomolecule solutions were measured. At pH 7, the ability of various biomolecules to reactively absorb ozone was in the following sequence: thiosulfate > ascorbate > cysteine approximately methionine > glutathione. The rates of reactive absorption under a variety of...

journal_title：Archives of biochemistry and biophysics

authors： Kanofsky JR,Sima PD

更新日期：1995-01-10 00:00:00

abstract：:NADP-malate dehydrogenase activity, the ratio of NADPH to NADP, and thioredoxin redox state in Zea mays chloroplasts were determined after various treatments. Following transfer from dark to light, NADP-malate dehydrogenase was activated more than 20-fold within 10 min while the proportion of pyridine nucleotide as NA...

journal_title：Archives of biochemistry and biophysics

authors： Rebeille F,Hatch MD

更新日期：1986-08-15 00:00:00

abstract：:In the absence of added calcium, inhibition of NAD-specific isocitrate dehydrogenase by ATP occurred without ADP (I0.5 = 1.8 mM) and with 0.2 mM ADP3- (I0.5 = 1.0 mM) at subsaturating substrate concentrations at pH 7.4. Inhibition by ATP was competitive with NAD+ in the presence and absence of ADP and was not reversed...

journal_title：Archives of biochemistry and biophysics

authors： Gabriel JL,Milner R,Plaut GW

更新日期：1985-07-01 00:00:00

abstract：:Salicylhydroxamic acid reductase, which catalyzes the reduction of salicylhydroxamic acid to salicylamide, was purified from rat liver cytosol. The purification procedure consisted of fractionation with ammonium sulfate, chromatography with Phenyl-Toyopearl 650, DEAE-cellulose, hydroxyapatite, and Sephadex G-200, and ...

journal_title：Archives of biochemistry and biophysics

authors： Katsura H,Kitamura S,Tatsumi K

更新日期：1993-05-01 00:00:00

abstract：:The serine carboxylesterase from horse serum was characterized by amino acid composition, peptide mapping, molecular and subunit weights, and sequencing of the amino acids around the essential serine residue at the active site. A protocol was developed for using reversed-phase high-performance liquid chromatography as...

journal_title：Archives of biochemistry and biophysics

authors： Torres JL,Rush RS,Main AR

更新日期：1988-11-15 00:00:00

abstract：:Diquat is a hepatotoxin whose toxicity in vivo and in vitro is mediated by redox cycling and greatly enhanced by pretreatment with 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU), an inhibitor of glutathione reductase. The mechanism by which redox cycling mediates diquat cytotoxicity is unclear, however. Here, we have att...

journal_title：Archives of biochemistry and biophysics

authors： Sandy MS,Moldeus P,Ross D,Smith MT

更新日期：1987-11-15 00:00:00

abstract：:Cytochrome P450 reductase was purified to homogeneity from cell suspension cultures of the opium poppy Papaver somniferum, the enzyme was characterized (K(m) cytochrome c, 8.3 microM; K(m) NADPH, 4.2 microM; pH optimum, 8.0; M(r), 80 kDa), and the amino acid sequence of internal peptides was determined. Partial cDNA c...

journal_title：Archives of biochemistry and biophysics

authors： Rosco A,Pauli HH,Priesner W,Kutchan TM

更新日期：1997-12-15 00:00:00

abstract：:The mitochondrial ATP synthase/ATPase (F0F1 ATPase) is perhaps the most complex enzyme known. In animal systems it consists of a minimum of 11 different polypeptide chains, 10 (or more) of which appear to be essential for function, and 1 called the "ATPase inhibitor peptide" which is involved in regulation. Recent stu...

journal_title：Archives of biochemistry and biophysics

authors： Schwerzmann K,Pedersen PL

更新日期：1986-10-01 00:00:00

abstract：:Phospholipid transfer protein catalyzes the transfer of phospholipids between bilayer membranes. A general model is developed for describing the kinetics of this process. While previous models derive detailed expressions only for the initial rate of transfer from donor to acceptor membranes, this model takes into acco...

journal_title：Archives of biochemistry and biophysics

authors： Yoshimura T,Welti R,Helmkamp GM Jr

更新日期：1988-11-01 00:00:00

abstract：:Although the relationship between hypercholesterolemia and oxidative stress has been extensively investigated, direct evidence regarding to the roles of cholesterol accumulation in the generations of reactive oxygen species (ROS) and apoptotic cell death under oxidative stress is lack. In this study, we investigated p...

journal_title：Archives of biochemistry and biophysics

authors： Lee W,Xu M,Li Y,Gu Y,Chen J,Wong D,Fung PC,Shen J

更新日期：2011-10-01 00:00:00

abstract：:Photoinduced reduction of methylviologen (MV2+) by ethylenediaminetetraacetate (EDTA3-), which was sensitized by thiacarbocyanine dyes having long alkyl chains (C+m-n) embedded in palmitoyl lysophosphatidylcholine micelle and dipalmitoyl phosphatidylcholine liposomal membrane, was carried out. The formation rate of re...

journal_title：Archives of biochemistry and biophysics

authors： Nakagaki M,Komatsu H,Handa T

更新日期：1986-09-01 00:00:00

abstract：:Cellular distribution in rat livers of the mannan-binding protein, a liver lectin specific for mannose and N-acetylglucosamine was investigated. Estimation of mannan binding activity of isolated cells demonstrated that more than 99% of the mannan binding activity recovered from whole livers was derived from hepatocyte...

journal_title：Archives of biochemistry and biophysics

authors： Mori K,Kawasaki T,Yamashina I

更新日期：1983-04-15 00:00:00