Two coding regions closely linked to the rat apolipoprotein E gene: nucleotide sequences of rat apolipoprotein C-I and ECL cDNA.

abstract：:The insulin receptor from rat skeletal muscle was characterized. Treatment of muscle membranes with the photoactive insulin analog, 125I[N-epsilonB29-monoazidobenzoyl]-insulin revealed a single protein band of 135,000 Da, the alpha subunit. Iodination of total membrane protein followed by Triton X-100 solubilization a...

journal_title：Archives of biochemistry and biophysics

authors： Dubler RE,Whipple JH,Larner J

更新日期：1985-01-01 00:00:00

abstract：:Tetrahydro-dUMP, an analog of the putative transition state in aminohydrolysis of deoxycytidine monophosphate (dCMP) inhibits the allosteric enzyme deoxycytidylate aminohydrolase with high affinity. The inhibition is reversible, and its kinetics is consistent with the analog binding at the substrate site only to one a...

journal_title：Archives of biochemistry and biophysics

authors： Nucci R,Febbraio F,Piccialli G,de Napoli L,Vaccaro C,Rossi M,Whitehead EP

更新日期：1994-04-01 00:00:00

abstract：:Epidemiological evidence strongly suggests that lycopene consumption contributes to prostate cancer risk reduction. Preclinical studies show that lycopene acts via different mechanisms, which have the potential to cooperate in reducing the proliferation of normal and cancerous prostate epithelial cells, in reducing DN...

journal_title：Archives of biochemistry and biophysics

authors： Wertz K,Siler U,Goralczyk R

更新日期：2004-10-01 00:00:00

abstract：:A deficiency in microsomal glucose-6-phosphatase (G6Pase) activity causes glycogen storage disease type 1 (GSD-1), a clinically and biochemically heterogeneous group of diseases. It has been suggested that catalysis by G6Pase involves multiple components, with defects in the G6Pase catalytic unit causing GSD-1a and de...

journal_title：Archives of biochemistry and biophysics

authors： Pan CJ,Lei KJ,Chen H,Ward JM,Chou JY

更新日期：1998-10-01 00:00:00

abstract：:The tertiary structure model of EF-Tu predicts that the amino acid sequence Val-Asp-His-Gly-Lys-Thr-Thr-Leu (residues 20-27) forms a pocket that binds the pyrophosphate group. To test this model we used site-directed mutagenesis to produce forms of EF-Tu altered in this region. The following mutations were constructed...

journal_title：Archives of biochemistry and biophysics

authors： Hwang YW,McCabe PG,Innis MA,Miller DL

更新日期：1989-11-01 00:00:00

abstract：:A fraction containing a protein-glycogen complex was isolated from rat skeletal muscle in order to study the effect of hypothyroidism on phosphorylase activation in this structural and functional unit of the glycogenolytic process. The total activities of phosphorylase and phosphorylase phosphatase in euthyroids and h...

journal_title：Archives of biochemistry and biophysics

authors： Leijendekker WJ,Edauw P,van Hardeveld C,Simonides WS

更新日期：1989-10-01 00:00:00

abstract：:Candida wickerhamii NRRL Y-2563 produced a cell-bound beta-glucosidase when grown in complex media containing 50 g of cellobiose per liter. The majority of the enzyme was located on the cell surface and was released into the supernatant upon treatment of intact cells with Zymolyase 60,000. Only about 10% of the total ...

journal_title：Archives of biochemistry and biophysics

authors： Freer SN

更新日期：1985-12-01 00:00:00

abstract：:Under physiological conditions the uptake of transferrin-bound iron by reticulocytes involves transferrin binding to membrane receptors followed by endocytosis, release of iron from the transferrin within endosomes, and recycling of apotransferrin to the cell surface. However, as shown in the present work, iron uptake...

journal_title：Archives of biochemistry and biophysics

authors： Hodgson LL,Quail EA,Morgan EH

更新日期：1994-01-01 00:00:00

abstract：:Numerous conditions induce expression of manganese-containing superoxide dismutase (MnSOD) in mammalian cells. The reported inducers of MnSOD are all agents that activate two transcription factors, AP-1 and NF kappa B, but several reports have suggested that MnSOD induction relies solely on NF kappa B. We investigated...

journal_title：Archives of biochemistry and biophysics

authors： Borrello S,Demple B

更新日期：1997-12-15 00:00:00

abstract：:This work studied the effect of cholesteryl hemisuccinate incorporation on membrane fluidity and on the kinetics of oxygen diffusion at different depths in the erythrocyte membrane. Cholesterol concentration in the membrane was expressed as the cholesterol-protein ratio (C/Pt). The membrane fluidity, as assessed by a ...

journal_title：Archives of biochemistry and biophysics

authors： Dumas D,Muller S,Gouin F,Baros F,Viriot ML,Stoltz JF

更新日期：1997-05-01 00:00:00

abstract：:The stabilities of bilirubin (BR) glucuronide, monoglucuronide (BMG), and diglucuronide (BDG) were studied under various conditions by HPLC. In aqueous media, BMG showed a pronounced lability and was easily transformed into equimolar BDG and BR. It was proved by direct analysis of tetrapyrrole isomers that BDG and BR ...

journal_title：Archives of biochemistry and biophysics

authors： Adachi S,Uesugi T,Kamisaka K

更新日期：1985-09-01 00:00:00

abstract：:Characterization of aldose reductase purified from human placenta confirms that activation, as first analyzed in detail for the bovine enzyme, also occurs in humans. Routinely between 5 and 20% of the aldose reductase activity freshly purified from human placenta exhibits kinetic properties and insensitivity to aldose...

journal_title：Archives of biochemistry and biophysics

authors： Grimshaw CE,Lai CJ

更新日期：1996-03-01 00:00:00

abstract：:The changes in retinoid metabolism have been documented in liver cirrhosis. However, the dynamic alterations in levels of this vitamin between circulation and liver during development of the liver cirrhosis are not well understood. The aim of this study was to measure retinoids in the liver and circulation in parallel...

journal_title：Archives of biochemistry and biophysics

authors： Natarajan SK,Thomas S,Ramachandran A,Pulimood AB,Balasubramanian KA

更新日期：2005-11-15 00:00:00

abstract：:Lipoxygenases react with hydroperoxy fatty acids and catalyze dioxygenase or dehydrase (leukotriene A4 (LTA4) synthase) types of reactions. In the present investigation we studied the mechanism of reaction of the purified porcine leukocyte 12S-lipoxygenase with 15S-hydroperoxyeicosatetraenoic acid (15S-HPETE). Oxygen-...

journal_title：Archives of biochemistry and biophysics

authors： Brash AR,Yokoyama C,Oates JA,Yamamoto S

更新日期：1989-09-01 00:00:00

abstract：:The conditions for accurately determining distance constraints from TrNOESY data on a small ligand (3'CMP) bound to a small protein (RNase A, <14 kDa) are described. For small proteins, normal TrNOESY conditions of 10:1 ligand:protein or greater can lead to inaccurate structures for the ligand-bound conformation due t...

journal_title：Archives of biochemistry and biophysics

authors： Lee YC,Jackson PL,Jablonsky MJ,Muccio DD

更新日期：2007-07-01 00:00:00

abstract：:Chlorophyllase catalyzes the initial step in the degradation of chlorophyll and plays a key role in leaf senescence and fruit ripening. Here, we report the cloning of chlorophyllase from Triticum aestivum (wheat) and provide a detailed mechanistic analysis of the enzyme. Purification of recombinant chlorophyllase from...

journal_title：Archives of biochemistry and biophysics

authors： Arkus KA,Cahoon EB,Jez JM

更新日期：2005-06-15 00:00:00

abstract：:Diastolic dysfunction is a clinically significant problem for patients with diabetes and often reflects increased ventricular stiffness. Attached cross-bridges contribute to myocardial stiffness and produce short-range forces, but it is not yet known whether these forces are altered in diabetes. In this study, we test...

journal_title：Archives of biochemistry and biophysics

authors： Chung CS,Mitov MI,Callahan LA,Campbell KS

更新日期：2014-06-15 00:00:00

abstract：:Upon treatment of Papaveracea cells with fungal elicitors, the biosynthesis of benzo[c]phenanthridine alkaloids is induced. Dihydrobenzophenanthridine oxidase, which catalyzes a later step in the biogenesis of these alkaloids, is one of the enzymes whose activity is elevated in the process. Here we report the 211-fold...

journal_title：Archives of biochemistry and biophysics

authors： Arakawa H,Clark WG,Psenak M,Coscia CJ

更新日期：1992-11-15 00:00:00

abstract：:Recently our group synthesized a new class of melanins obtained by the tyrosinase-catalyzed oxidation of opioid peptides (opiomelanins). Owing to the presence of the peptide moiety such pigments exhibit high solubility in hydrophilic solvents, which allows spectroscopic investigations. In particular, the absence of so...

journal_title：Archives of biochemistry and biophysics

authors： Mosca L,De Marco C,Fontana M,Rosei MA

更新日期：1999-11-01 00:00:00

abstract：:FtsZ (Filamentous temperature sensitivity Z) cell division protein from Escherichia coli binds the fluorescence probe DAPI. Bundling of FtsZ was facilitated in the presence of DAPI, and the polymers in solution remained polymerized longer time than the protofilaments formed in the absence of DAPI. DAPI decreased both ...

journal_title：Archives of biochemistry and biophysics

authors： Nova E,Montecinos F,Brunet JE,Lagos R,Monasterio O

更新日期：2007-09-15 00:00:00

abstract：:Rabbit anti-tyrosinase antibodies were used to study the abundance, processing, and degradation of tyrosinase in murine (Cloudman) melanoma cells. The polyclonal antibodies precipitated low-molecular-weight (68,000 and 70,000) and high-molecular-weight (78,000 and 80,000) tyrosinases that had a precursor-product relat...

journal_title：Archives of biochemistry and biophysics

authors： Halaban R,Pomerantz SH,Marshall S,Lerner AB

更新日期：1984-04-01 00:00:00

abstract：:Most important processes in cell life are regulated by calcium (Ca2+). A number of mechanisms have thus been developed to maintain the concentration of free Ca2+ inside cells at the level (100-200nM) necessary for the optimal operation of the targets of its regulatory function. The systems that move Ca2+ back and fort...

journal_title：Archives of biochemistry and biophysics

authors： Di Leva F,Domi T,Fedrizzi L,Lim D,Carafoli E

更新日期：2008-08-01 00:00:00

abstract：:The conversion of the precursor of apolipoprotein A-I (proapoA-I) to apolipoprotein A-I (apoA-I) is known to occur extracellularly by an enzyme that has been shown to be present in plasma. The hepatocarcinoma-derived cell line Hep G2, when grown in culture, secretes proapoA-I. We now show that this cell line also secr...

journal_title：Archives of biochemistry and biophysics

authors： Edelstein C,Kaiser M,Piras G,Scanu AM

更新日期：1988-11-15 00:00:00

abstract：:The influence of oxidative stress on agonist-stimulated changes of intracellular free calcium and inositol trisphosphate in the neurosecretory PC12 cell line was investigated. The oxidant H2O2 modulated the bradykinin-induced calcium signal by decreasing the initial peak and the plateau phase in the same manner as tet...

journal_title：Archives of biochemistry and biophysics

authors： Klyszcz-Nasko H,Richter-Landsberg C,Beyersmann D

更新日期：1993-11-01 00:00:00

abstract：:Previous studies showed that vegetative cells of Dictyostelium discoideum make a cysteine proteinase called proteinase-1, which contains multiple residues of GlcNAc-1-P linked directly to peptidyl serines. As a prelude to understanding the function of this novel carbohydrate modification, we purified and extensively c...

journal_title：Archives of biochemistry and biophysics

authors： Mehta DP,Etchison JR,Freeze HH

更新日期：1995-08-01 00:00:00

abstract：:60S ribosomal protein L35 (RPL35) is an important component of the 60S ribosomal subunit and has a role in protein translation and endoplasmic reticulum (ER) docking. However, few studies have investigated RPL35 in eukaryotes and much remains to be learned. Here, we analyzed the function of RPL35 in β-casein (CSN2) sy...

journal_title：Archives of biochemistry and biophysics

authors： Jiang N,Hu L,Liu C,Gao X,Zheng S

更新日期：2015-10-01 00:00:00

abstract：:Based on previous findings of increased nitric oxide synthase (NOS) expression in human gliomas (4), we hypothesized that peroxynitrite, a highly reactive metabolite of nitric oxide (NO) and superoxide (O(*-)(2)), might be increased in these tumors in vivo. Here we demonstrate that nitrotyrosine (a footprint of peroxy...

journal_title：Archives of biochemistry and biophysics

authors： Cobbs CS,Samanta M,Harkins LE,Gillespie GY,Merrick BA,MacMillan-Crow LA

更新日期：2001-10-15 00:00:00

abstract：:Diglycyl triperoxodivanadate [V2O2(O2)3(Gly H)2(H2O)2], a synthetic compound with mu-peroxo-bridge derived from H2O2 and vanadate, oxidized bromide to a bromination-competent intermediate in phosphate buffer and physiological pH. This is in contrast to the requirement of acid medium with H2O2 as the oxidant. Addition ...

journal_title：Archives of biochemistry and biophysics

authors： Rao AV,Islam NS,Ramasarma T

更新日期：1997-06-15 00:00:00

abstract：:The mechanisms underlying the inhibition of bile acid-induced apoptosis by cyclic AMP (cAMP) were studied in 24-h-cultured rat hepatocytes. Taurolithocholate 3-sulfate (TLCS, 100 micromol/l) led to a sustained activation of mitogen activated protein (MAP) kinases (JNK, p38(MAPK), and ERKs), dephosphorylation of protei...

journal_title：Archives of biochemistry and biophysics

authors： Graf D,Reinehr R,Kurz AK,Fischer R,Häussinger D

更新日期：2003-07-01 00:00:00

abstract：:The important characteristic of novel Mimosa pudica tubulin is its ability to bind colchicine only when dithiothreitol is included in the isolation buffer, indicating the involvement of sulfhydryl groups in colchicine binding. Modification of sulfhydryl groups by a sulfhydryl modifying agent also affects the normal as...

journal_title：Archives of biochemistry and biophysics

authors： Roychaudhuri A,Biswas S

更新日期：1992-05-01 00:00:00