Reductive cleavage of Xaa-proline peptide bonds by mild alkaline borohydride treatment employed to release O-glycosidically linked carbohydrate units of glycoproteins.

abstract：:Incubation of Novikoff rat hepatoma cells; mouse L929, P388 and L1210 cells; and Chinese hamster ovary cells with sulfhydryl reagents, such as p-hydroxymercuribenzoate or p-hydroxymercuribenzenesulfonate, reduced the zero-trans influx of uridine in a concentration-dependent manner. The sensitivity of uridine transport...

journal_title：Archives of biochemistry and biophysics

authors： Plagemann PG,Wohlhueter RM

更新日期：1984-09-01 00:00:00

abstract：:Triosephosphate isomerase from rabbit has 5 Cys and 2 Met, while triosephosphate isomerase from yeast has 2 Cys (present in the rabbit enzyme in equivalent positions) and no Met. Since chloramine-T oxidizes Cys and Met, we determined the effect it has on the activity and structure of both enzymes. The activity of trio...

journal_title：Archives of biochemistry and biophysics

authors： Zubillaga RA,Pérez-Montfort R,Gómez-Puyou A

更新日期：1994-09-01 00:00:00

abstract：:An inhibitor protein of the catalytic subunit of the cyclic 3',5'-AMP-dependent protein kinase from the nematode Ascaris suum was isolated and characterized. The molecular weight of the inhibitor was estimated as 28,000 by electrophoresis under denaturing conditions and as 30,000 by gel permeation chromatography on Su...

journal_title：Archives of biochemistry and biophysics

authors： Hoffmann R,Jung S,Hofer HW

更新日期：1992-09-01 00:00:00

abstract：:In the absence of added calcium, inhibition of NAD-specific isocitrate dehydrogenase by ATP occurred without ADP (I0.5 = 1.8 mM) and with 0.2 mM ADP3- (I0.5 = 1.0 mM) at subsaturating substrate concentrations at pH 7.4. Inhibition by ATP was competitive with NAD+ in the presence and absence of ADP and was not reversed...

journal_title：Archives of biochemistry and biophysics

authors： Gabriel JL,Milner R,Plaut GW

更新日期：1985-07-01 00:00:00

abstract：:Hemorrhagic toxin f (HT-f) was isolated from Crotalus atrox (Western Diamondback Rattlesnake) venom by a five-step purification procedure. Homogeneity was established by the formation of a single band in acrylamide gel electrophoresis, isoelectric focusing, and sodium dodecyl sulfate (SDS)-electrophoresis. HT-f has a ...

journal_title：Archives of biochemistry and biophysics

authors： Nikai T,Mori N,Kishida M,Sugihara H,Tu AT

更新日期：1984-06-01 00:00:00

abstract：:Purified NAD-malic enzyme from Ascaris suum is rapidly inactivated by the arginine reagent, 2,3-butanedione, and this inactivation is facilitated by 30 mM borate. Determination of the inactivation rate as a function of butanedione concentration suggests a second-order process overall, which is first order in butanedio...

journal_title：Archives of biochemistry and biophysics

authors： Rao GS,Kong CT,Benjamin RC,Harris BG,Cook PF

更新日期：1987-05-15 00:00:00

abstract：:Hepatic flavin-containing monooxygenases catalyze NADPH-dependent oxygenation of a wide variety of drugs that possess a nucleophilic heteroatom. Two forms of these enzymes (form 1 and 2) have been isolated from rabbit liver microsomes and partially characterized (Ozols, J., 1989, Biochem. Biophys. Res. Commun. 163, 49...

journal_title：Archives of biochemistry and biophysics

authors： Ozols J

更新日期：1991-10-01 00:00:00

abstract：:This study has been done to know what kind of factors in plasmas and processes on cells induce plasma gene transfection. We evaluated the contribution weight of three groups of the effects and processes, i.e. electrical, chemical and biochemical ones, inducing gene transfection. First, the laser produced plasma (LPP) ...

journal_title：Archives of biochemistry and biophysics

authors： Jinno M,Ikeda Y,Motomura H,Kido Y,Satoh S

更新日期：2016-09-01 00:00:00

abstract：:Phagocytes generate superoxide (O2-.) and hydrogen peroxide (H2O2) and their interaction in an iron-catalyzed reaction to form hydroxyl radicals (OH.) (Haber-Weiss reaction) has been proposed. Deferoxamine chelates iron in a catalytically inactive form, and thus inhibition by deferoxamine has been employed as evidence...

journal_title：Archives of biochemistry and biophysics

authors： Klebanoff SJ,Waltersdorph AM

更新日期：1988-08-01 00:00:00

abstract：:Several experiments were carried out to study the difference between two isozymes (alpha(+) and alpha) of (Na+ + K+)-ATPase in the conformational equilibrium. Rat brain (Na+ + K+)-ATPase was much more thermolabile than the kidney enzyme. Both enzymes were protected from heat inactivation not only by Na+ and K+, but al...

journal_title：Archives of biochemistry and biophysics

authors： Matsuda T,Iwata H

更新日期：1988-06-01 00:00:00

abstract：:The effects of nonlamellar-prone lipids, diacylglycerol and phosphatidylethanolamine (PE), on the kinetic association of SecA with model membranes were examined by measuring changes in the intrinsic emission fluorescence with a stopped-flow apparatus. Upon interaction with standard liposomes composed of 50 mol% dioleo...

journal_title：Archives of biochemistry and biophysics

authors： Ahn T,Kim JS,Lee BC,Yun CH

更新日期：2001-11-01 00:00:00

abstract：:Artemia trypsin-like proteinase has been reported previously to be highly inhibited in the embryo (B. Ezquieta and C.G. Vallejo (1985) Comp. Biochem. Physiol. 82B, 731-736). We report now that Artemia lipovitellin, the major storage protein complex, inhibits the proteinase. We have carried out an in vitro study of the...

journal_title：Archives of biochemistry and biophysics

authors： Ezquieta B,Vallejo CG

更新日期：1986-11-01 00:00:00

abstract：:Protein synthesis and lipid peroxidation were evaluated in rat liver slices incubated in the presence of oxidants and protein synthesis inhibitors. Protein synthesis by rat liver slices was evaluated by [3H]leucine incorporation into the trichloroacetic acid (TCA)-insoluble material, and lipid peroxidation was evaluat...

journal_title：Archives of biochemistry and biophysics

authors： Fraga CG,Zamora R,Tappel AL

更新日期：1989-04-01 00:00:00

abstract：:Bidentate cobalt(III)tetraamine adenosine triphosphate [Co(NH3)4ATP] was investigated as an inhibitor of the beef heart mitochondrial F1-ATPase. The compound was found to have a mixed noncompetitive mechanism with a Ki of 0.4 mM and an alpha of 1.4 during ATP hydrolysis. Co(NH3)4ATP also noncompetitively inhibited ATP...

journal_title：Archives of biochemistry and biophysics

authors： Steinke L,Schuster SM

更新日期：1985-05-01 00:00:00

abstract：:Rat Leydig cells in primary culture were used as a model system to investigate the effects of human chorionic gonadotropin (hCG) and dibutyryl cyclic AMP (Bt2cAMP) on the synthesis of cholesterol side chain cleavage cytochrome P-450 (cytochrome P-450scc) and the iron-sulfur protein, adrenodoxin. Leydig cells isolated ...

journal_title：Archives of biochemistry and biophysics

authors： Anderson CM,Mendelson CR

更新日期：1985-05-01 00:00:00

abstract：:Under a variety of conditions, the permeability of the inner mitochondrial membrane to small solutes can be nonselectively increased. A classic mitochondrial permeability transition (MPT) was originally identified based on its dependence on matrix Ca2+ and its extreme sensitivity to cyclosporin A (CsA). It is now clea...

journal_title：Archives of biochemistry and biophysics

authors： Kushnareva YE,Haley LM,Sokolove PM

更新日期：1999-03-01 00:00:00

abstract：:In order to characterize cellular retinoic acid-binding protein (CRABP) from chick embryos, we resolved the [3H]RA-binding activity of embryo extracts by Mono Q anion-exchange chromatography. Fractions containing [3H]RA-binding proteins eluted in two peaks at 7 and 10 min with the latter showing 10-fold higher activit...

journal_title：Archives of biochemistry and biophysics

authors： Venepally P,Reddy LG,Sani BP

更新日期：1996-12-15 00:00:00

abstract：:The relative reactivities of lysine residues of tropomyosin complexed with troponin have been measured in order to locate the binding site of troponin on tropomyosin in a complex between the two native proteins. The lysines were labeled with acetic anhydride using a competitive labeling procedure and the relative reac...

journal_title：Archives of biochemistry and biophysics

authors： Hitchcock-DeGregori SE,Lewis SF,Mistrik M

更新日期：1988-08-01 00:00:00

abstract：:Replication of the human immunodeficiency virus type 1 (HIV-1) is inhibited by interferons (IFNs), in part through activity of the IFN-inducible protein kinase PKR. To escape this antiviral effect, HIV-1 has developed strategies for blocking PKR function. We have previously shown that the HIV-1 Tat protein can associa...

journal_title：Archives of biochemistry and biophysics

authors： Cai R,Carpick B,Chun RF,Jeang KT,Williams BR

更新日期：2000-01-15 00:00:00

abstract：:Excessive iron accumulation in the heart can lead to iron overload cardiomyopathy (IOC), the leading cause of death in hemochromatosis patients. Current understanding regarding the mechanism by which iron overload causes a deterioration in cardiac performance, mitochondrial dysfunction, and impaired mitochondrial dyna...

journal_title：Archives of biochemistry and biophysics

authors： Sumneang N,Siri-Angkul N,Kumfu S,Chattipakorn SC,Chattipakorn N

更新日期：2020-02-15 00:00:00

abstract：:Peroxynitrite, a reactive cytotoxic species generated by the reaction of superoxide with nitric oxide, rapidly oxidizes phenylaminoethyl selenide (PAESe) and its para-substituted derivatives with second-order rate constants ranging from 900 to 3000 M(-1) s(-1) at neutral pH (pH 7.0) and 25 degrees C. These values are ...

journal_title：Archives of biochemistry and biophysics

authors： Woznichak MM,Overcast JD,Robertson K,Neumann HM,May SW

更新日期：2000-07-15 00:00:00

abstract：:Incubation of rat liver nuclear envelopes with [gamma-32P]ATP resulted in the synthesis of phosphatidylinositol-[4-32P]phosphate (PIP). Degradation of endogenously labeled PIP was observed upon the dilution of the labeled ATP with an excess of unlabeled ATP. This degradation was most rapid in the presence of EDTA, and...

journal_title：Archives of biochemistry and biophysics

authors： Smith CD,Wells WW

更新日期：1984-12-01 00:00:00

abstract：:Pyruvate dehydrogenase (E1), an alpha(2)beta(2) tetramer, catalyzes the oxidative decarboxylation of pyruvate and reductive acetylation of lipoyl moieties of the dihydrolipoamide acetyltransferase. The roles of betaW135, alphaP188, alphaM181, alphaH15, and alphaR349 of E1 determined by kinetic analysis were reassessed...

journal_title：Archives of biochemistry and biophysics

authors： Korotchkina LG,Ciszak EM,Patel MS

更新日期：2004-09-15 00:00:00

abstract：:L-Tryptophan is an essential amino acid for mammals. It is utilized not only for protein synthesis but also for the biosynthesis of serotonin and melatonin by the serotonin pathway as well as nicotinamide adenine dinucleotide by the kynurenine pathway. Although the kynurenine pathway is responsible for the catabolism ...

journal_title：Archives of biochemistry and biophysics

authors： Geng J,Liu A

更新日期：2014-02-15 00:00:00

abstract：:Site-mutations were introduced into putative cation binding site 1 of the H,K-ATPase at glu-797, thr-825, and glu-938. The side chain oxygen of each was not essential but the mutations produced different activation and inhibition kinetics. Site mutations thr-825 (ala, leu) and glu-938 (ala, gln) modestly decreased the...

journal_title：Archives of biochemistry and biophysics

authors： Rulli SJ,Louneva NM,Skripnikova EV,Rabon EC

更新日期：2001-03-01 00:00:00

abstract：:Lubrol-solubilized rabbit thrombomodulin has been examined by equilibrium sedimentation in buffers that include sufficient D2O to make the detergent neutrally buoyant. Data were acquired at rotor speeds from 12,000 to 28,000 rpm from two thrombomodulin preparations, at protein concentrations from 0.01 to 0.07%, and in...

journal_title：Archives of biochemistry and biophysics

authors： Winnard PT,Esmon CT,Laue TM

更新日期：1989-02-15 00:00:00

abstract：:As a means of comparing the functional properties of an enzyme in dilute solution in vitro with those for the same enzyme acting in its normal cellular environment, a study was conducted with 4-substituted pyrazoles as inhibitors of rat liver alcohol dehydrogenase in vitro and ethanol oxidation in isolated rat hepatoc...

journal_title：Archives of biochemistry and biophysics

authors： Cornell NW,Hansch C,Kim KH,Henegar K

更新日期：1983-11-01 00:00:00

abstract：:An acid phosphatase from Brassica nigra (black mustard) leaf petiole cell-suspension cultures has been purified 1633-fold to a final specific activity of 1225 (mumols orthophosphate produced/min)/mg protein and near homogeneity. The native protein was a glycosylated monomer having a molecular mass of 60 kDa and a pI o...

journal_title：Archives of biochemistry and biophysics

authors： Duff SM,Lefebvre DD,Plaxton WC

更新日期：1991-04-01 00:00:00

abstract：:CYP27A1, an enzyme with several important roles in cholesterol homeostasis and vitamin D₃ metabolism, has been ascribed anti-atherogenic properties. This study addresses an important problem regarding how this enzyme, involved in cholesterol metabolism in the liver and peripheral tissues, is regulated. Our results ide...

journal_title：Archives of biochemistry and biophysics

authors： Norlin M,Pettersson H,Tang W,Wikvall K

更新日期：2011-02-15 00:00:00

abstract：:Pentobarbital, a general anesthetic and non-genotoxic carcinogen, can induce gene expression by activating transcription. In the Drosophila glutathione S-transferase D21 (gstD21) gene, pentobarbital's regulatory influence extends to the level of mRNA turnover. Transcribed from an intronless gene, gstD21 mRNA is intrin...

journal_title：Archives of biochemistry and biophysics

authors： Akgül B,Tu CP

更新日期：2007-03-01 00:00:00