Investigation of plasma induced electrical and chemical factors and their contribution processes to plasma gene transfection.

abstract：:The pH dependence of the activity of Cu,Zn superoxide dismutases from bovine erythrocytes and shark liver was studied by pulse radiolysis in both the native enzymes and those chemically modified at lysine side chains. The study was aimed at identifying the residues responsible for the activity decrease at pH > 9, obse...

journal_title：Archives of biochemistry and biophysics

authors： Polticelli F,O'Neill P,Costanzo S,Lania A,Rotilio G,Desideri A

更新日期：1995-08-01 00:00:00

abstract：:Synephrinase, an enzyme catalyzing the conversion of (-)-synephrine into p-hydroxyphenylacetaldehyde and methylamine, was purified to apparent homogeneity from the cell-free extracts of Arthrobacter synephrinum grown on (+/-)-synephrine as the sole source of carbon and nitrogen. A 40-fold purification was sufficient t...

journal_title：Archives of biochemistry and biophysics

authors： Manne V,Kutty KR,Pillarisetti SR

更新日期：1986-07-01 00:00:00

abstract：:The regulation of the plasma membrane Ca2+ pump by hormones via phosphorylation in intact cells has not been clearly established. We now present evidence that the Ca2+ pump is phosphorylated on both serine and threonine residues in unstimulated and stimulated cultured rat aortic endothelial cells. Among the stimuli te...

journal_title：Archives of biochemistry and biophysics

authors： Wang KK,Du YS,Diglio C,Tsang W,Kuo TH

更新日期：1991-08-15 00:00:00

abstract：:Bovine collateral ligament synthesized a 35S-labeled large proteoglycan species which eluted with a Kav of approximately 0.27 on Sepharose CL-2B and contained only chondroitin sulfate chains with a molecular mass of approximately 32 kDa. Fluorography of the 35S-labeled core proteins derived from the large ligament pro...

journal_title：Archives of biochemistry and biophysics

authors： Campbell MA,Tester AM,Handley CJ,Checkley GJ,Chow GL,Cant AE,Winter AD,Cain WE

更新日期：1996-05-15 00:00:00

abstract：:A novel series of nonpeptidic compounds structurally related to the known anticholesteremic thyropropic acid were found to inhibit Escherichia coli peptide deformylase (PDF), with IC50 values in the low-micromolar range. Kinetic analysis of [4-(4-hydroxyphenoxy)-3,5-diiodophenyl]acetic acid reveals competitive inhibit...

journal_title：Archives of biochemistry and biophysics

authors： Jayasekera MM,Kendall A,Shammas R,Dermyer M,Tomala M,Shapiro MA,Holler TP

更新日期：2000-09-15 00:00:00

abstract：:Hyaluronidase from fresh human serum was purified to apparent homogeneity in a two-step procedure. Potent serum inhibitors of hyaluronidase activity were removed during the course of the purification. Isolation of the enzyme was expedited by the use of a newly devised ELISA-like assay. Enzyme activity was measured by ...

journal_title：Archives of biochemistry and biophysics

authors： Afify AM,Stern M,Guntenhöner M,Stern R

更新日期：1993-09-01 00:00:00

abstract：:Nitric oxide formation by GH3 pituitary cells is stimulated by depolarizing concentrations of K+ and by the L-channel Ca2+ agonist Bay kappa 8644 in an additive manner that depends on extracellular Ca2+. Ca(2+)-dependent NO formation at 100 microM arginine was inhibited 50% over a 30-min period by 5 microM NG-amino-L-...

journal_title：Archives of biochemistry and biophysics

authors： Cooper GR,Barr A,Wolff DJ

更新日期：1998-09-15 00:00:00

abstract：:The cardiomyopathy produced by the widely used anticancer drug adriamycin (ADR) is believed to be related to the production of reaction oxygen species and consumption of reduced glutathione (GSH) during redox cycling of the drug. Protection by vitamin E against the toxicity of ADR was studied in a model of compromised...

journal_title：Archives of biochemistry and biophysics

authors： Pascoe GA,Reed DJ

更新日期：1987-07-01 00:00:00

abstract：:A solution hybridization assay employing specific synthetic oligodeoxynucleotide probes was developed to study the regulation of intestinal calbindin-D28K mRNA. The technique is rapid and quantitative and eliminates the need for sample transfer, blotting, autoradiography, and densitometry. Following validation of the ...

journal_title：Archives of biochemistry and biophysics

authors： Fullmer CS

更新日期：1990-11-15 00:00:00

abstract：:Glucosidase I, the enzyme catalyzing the first step of N-linked oligosaccharide processing, has been purified from calf liver crude membranes [H. Hettkamp, G. Legler, and E. Bause, (1984) Eur. J. Biochem. 142, 85-90]. Binding experiments with concanavalin A-Sepharose suggest that glucosidase I is a glycoprotein with h...

journal_title：Archives of biochemistry and biophysics

authors： Schweden J,Borgmann C,Legler G,Bause E

更新日期：1986-07-01 00:00:00

abstract：:Lysosomes were isolated from Chinese hamster ovary cells by fractionation of a postnuclear supernatant in consecutive density gradients. By marker enzyme analysis, the preparation was 63-fold enriched for lysosomes compared to the homogenate and contained at most trace amounts of marker activities for plasma membrane,...

journal_title：Archives of biochemistry and biophysics

authors： Madden EA,Wirt JB,Storrie B

更新日期：1987-08-15 00:00:00

abstract：:The solution structure of native and systematically modified ovine submaxillary mucin (OSM) has been probed by proton NMR spectroscopic methods. Most of the resonances in the spectra have been tentatively assigned to the peptide and O-linked disaccharide, alpha-N-acetylneuraminic acid 2----6 alpha-N-acetylgalactosamin...

journal_title：Archives of biochemistry and biophysics

authors： Gerken TA

更新日期：1986-06-01 00:00:00

abstract：:Reductant-independent ATPase activity was initiated and studied for Azotobacter vinelandii and Clostridium pasteuranium nitrogenase proteins (Av1, Cp1 and Av2, Cp2, 1 designating the iron molybdenum protein and 2 the iron protein) and their heterologous crosses by two methods: (1) allowing dithionite to be depleted fr...

journal_title：Archives of biochemistry and biophysics

authors： Larsen C,Christensen S,Watt GD

更新日期：1995-11-10 00:00:00

abstract：:The properties of porcine platelet acyltransferases which catalyze the incorporation of unsaturated fatty acids into the 2 positions of phospholipids were compared with those of porcine liver microsomes and rat liver microsomes. There were significant differences in the relative rates of incorporation of acyl groups i...

journal_title：Archives of biochemistry and biophysics

authors： Inoue M,Murase S,Okuyama H

更新日期：1984-05-15 00:00:00

abstract：:The cis and trans conformations of the Xaa-Pro (Xaa: any amino acid) peptide bond are thermodynamically stable while other peptide bonds strongly prefer trans. The effect of proline cis-trans isomerization on protein binding has not been thoroughly investigated. In this study, computer simulations were used to calcula...

journal_title：Archives of biochemistry and biophysics

authors： Zhan YA,Ytreberg FM

更新日期：2015-06-01 00:00:00

abstract：:The native conformation of bovine retinal arrestin has been characterized by a variety of spectroscopic methods. The purified protein gives rise to a near uv absorption band centered at 279 nm which results from the absorbance of its 14 tyrosine and one tryptophan residue. The extinction coefficient for this absorptio...

journal_title：Archives of biochemistry and biophysics

authors： Kotake S,Hey P,Mirmira RG,Copeland RA

更新日期：1991-02-15 00:00:00

abstract：:The multifunctional tyrosine N-hydroxylase, cytochrome P450TYR (CYP79), from Sorghum bicolor catalyzing the conversion of tyrosine to p-hydroxyphenyl-acetaldoxime in the biosynthesis of the cyanogenic glucoside dhurrin, has been expressed in Escherichia coli using the isopropyl-beta-D-thiogalactopyranoside-inducible v...

journal_title：Archives of biochemistry and biophysics

authors： Halkier BA,Nielsen HL,Koch B,Møller BL

更新日期：1995-10-01 00:00:00

abstract：:The in vivo binding of platinum to metallothionein (MT) has been observed in rat tissues following injections of the cis and trans isomers of DDP (dichlorodiammineplatinum(II]. Platinum in either cis-DDP or trans-DDP does not directly induce MT; platinum-MT is produced by the replacement of previously bound zinc in th...

journal_title：Archives of biochemistry and biophysics

authors： Zelazowski AJ,Garvey JS,Hoeschele JD

更新日期：1984-02-15 00:00:00

abstract：:The tumor-promoting phorbol ester 4 beta-phorbol 12-myristate 13-acetate (PMA) inhibited thrombin-stimulated arachidonic acid (AA) release in rabbit and human platelets. PMA was effective over the same concentration range that activates protein kinase C in intact rabbit platelets: IC50 vs thrombin = 0.5 nM, greater th...

journal_title：Archives of biochemistry and biophysics

authors： Murayama T,Kajiyama Y,Takahashi A,Nomura Y

更新日期：1990-01-01 00:00:00

abstract：:The combination of phorbol 12-myristate 13-acetate (PMA) and ionomycin produces a dramatic increase in the incorporation of [2-3H]mannose into Glc3Man9GlcNAc2-P-P-dolichol and glycoprotein, and the induction of RNA and DNA synthesis in murine splenic B lymphocytes (B cells). The kinetics of the induction processes and...

journal_title：Archives of biochemistry and biophysics

authors： Rush JS,Snow EC,Waechter CJ

更新日期：1988-11-15 00:00:00

abstract：:The majority of hypertrophic cardiomyopathy mutations in (cTnT) occur within the alpha-helical tropomyosin binding TNT1 domain. A highly charged region at the C-terminal end of TNT1 unwinds to create a flexible "hinge". While this region has not been structurally resolved, it likely acts as an extended linker between ...

journal_title：Archives of biochemistry and biophysics

authors： Moore RK,Abdullah S,Tardiff JC

更新日期：2014-06-15 00:00:00

abstract：:Glycosphingolipids were extracted from human semen and purified. Based on the fluorometric assay of sphingosine, in spermatozoa a content of 4.4 +/- 0.9 nmol/10(8) cells of gangliosides and 22.1 +/- 1.7 nmol/10(8) cells of neutral glycosphingolipids was determined. Seminal plasma contained 4.1 +/- 0.6 nmol ganglioside...

journal_title：Archives of biochemistry and biophysics

authors： Ritter G,Krause W,Geyer R,Stirm S,Wiegandt H

更新日期：1987-09-01 00:00:00

abstract：:Electron spin lattice relaxation times (T1) and the phase memory times (Tm) were obtained for the synthetic melanin system from 3-hydroxytyrosine (dopa) by means of electron spin echo spectroscopy at 77 degrees K. Saturation behavior of the ESR spectra of melanins in melanin-containing tissue and of the synthetic mela...

journal_title：Archives of biochemistry and biophysics

authors： Okazaki M,Kuwata K,Miki Y,Shiga S,Shiga T

更新日期：1985-10-01 00:00:00

abstract：:Two novel medicinally important legume lectins from Bauhinia purpurea (BPL) and Wisteria floribunda (WFL) possessing extended sugar binding site were investigated for functional and conformational transitions using biochemical and biophysical techniques as well as bioinformatical tools. Homology model of BPL was const...

journal_title：Archives of biochemistry and biophysics

authors： Agrawal SB,Ghosh D,Gaikwad SM

更新日期：2019-02-15 00:00:00

abstract：:A radiochemical assay was developed for measuring branched-chain alpha-ketoacid dehydrogenase activity of Triton X-100 extracts of freeze-clamped rat liver. The proportion of active (dephosphorylated) enzyme was determined by measuring enzyme activities before and after activation of the complex with a broad-specifici...

journal_title：Archives of biochemistry and biophysics

authors： Harris RA,Powell SM,Paxton R,Gillim SE,Nagae H

更新日期：1985-12-01 00:00:00

abstract：:Previously human cytochrome P450 3A4 was efficiently and specifically photolabeled by the photoaffinity ligand lapachenole. One of the modification sites was identified as cysteine 98 in the B-C loop region of the protein [B. Wen, C.E. Doneanu, C.A. Gartner, A.G. Roberts, W.M. Atkins, S.D. Nelson, Biochemistry 44 (200...

journal_title：Archives of biochemistry and biophysics

authors： Wen B,Lampe JN,Roberts AG,Atkins WM,David Rodrigues A,Nelson SD

更新日期：2006-10-01 00:00:00

abstract：:Reduction-oxidation (redox) response is one of the most important biological phenomena. The concept introduced by Helmut Sies encouraged many researchers to examine oxidative stress under pathophysiological conditions. Our group has been interested in redox regulation under oxidative stress as well as glycobiology in ...

journal_title：Archives of biochemistry and biophysics

authors： Taniguchi N,Kizuka Y,Takamatsu S,Miyoshi E,Gao C,Suzuki K,Kitazume S,Ohtsubo K

更新日期：2016-04-01 00:00:00

abstract：:We have developed a protocol to purify apo-ovine (o) prostaglandin endoperoxide H(2) synthase-1 (PGHS-1) to homogeneity from ram seminal vesicles. The resulting apo enzyme can then be reconstituted with Co(3+)-protoporphyrin IX instead of Fe(3+)-protoporphyrin IX to produce a native-like, but functionally inert, enzym...

journal_title：Archives of biochemistry and biophysics

authors： Malkowski MG,Theisen MJ,Scharmen A,Garavito RM

更新日期：2000-08-01 00:00:00

abstract：:Interleukin-1 alpha (IL-1) stimulated the release of degraded proteoglycan from primary cultures of chondrocyte monolayers in a time- and dose-dependent fashion. Bafilomycin A1, a specific inhibitor of the vacuolar H(+)-ATPase, efficiently blocked acidification of the chondrocyte vacuolar system. Under these condition...

journal_title：Archives of biochemistry and biophysics

authors： Yocum SA,Lopresti-Morrow LL,Gabel CA,Milici AJ,Mitchell PG

更新日期：1995-02-01 00:00:00

abstract：:We have examined the effects of calcium and magnesium on both the structural characteristics and the self-association reaction of hyalin, a major protein component of the sea urchin extraembryonic matrix, the hyaline layer. In the absence of calcium, the circular dichroic spectrum revealed a protein possessing a high ...

journal_title：Archives of biochemistry and biophysics

authors： Rimsay R,Robinson JJ

更新日期：2003-06-15 00:00:00