当前位置： SCI文献检索 > ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS期刊下所有文献 > The cis conformation of proline leads to weaker binding of a p53 peptide to MDM2 compared to trans.

The cis conformation of proline leads to weaker binding of a p53 peptide to MDM2 compared to trans.

Abstract：

:The cis and trans conformations of the Xaa-Pro (Xaa: any amino acid) peptide bond are thermodynamically stable while other peptide bonds strongly prefer trans. The effect of proline cis-trans isomerization on protein binding has not been thoroughly investigated. In this study, computer simulations were used to calculate the absolute binding affinity for a p53 peptide (residues 17-29) to MDM2 for both cis and trans isomers of the p53 proline in position 27. Results show that the cis isomer of p53(17-29) binds more weakly to MDM2 than the trans isomer, and that this is primarily due to the difference in the free energy cost associated with the loss of conformational entropy of p53(17-29) when it binds to MDM2. The population of cis p53(17-29) was estimated to be 0.8% of the total population in the bound state. The stronger binding of trans p53(17-29) to MDM2 compared to cis may leave a minimal level of p53 available to respond to cellular stress. This study demonstrates that it is feasible to estimate the absolute binding affinity for an intrinsically disordered protein fragment binding to an ordered protein that are in good agreement with experimental results.

journal_name

Arch Biochem Biophys

journal_title

Archives of biochemistry and biophysics

authors

Zhan YA,Ytreberg FM

doi

10.1016/j.abb.2015.03.021

subject

Has Abstract

pub_date

2015-06-01 00:00:00

pages

22-9

eissn

0003-9861

issn

1096-0384

pii

S0003-9861(15)00157-5

journal_volume

575

pub_type

杂志文章

相关文献

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS文献大全
  • Manganese, calcium, and saccharide binding to concanavalin A, as studied by ultrafiltration.

    abstract：:The binding of the ligands Mn2+, Ca2+, and methyl alpha-D-glucopyranoside to concanavalin A, purified as described (A.J. Sophianopoulos and J.A. Sophianopoulos (1981) Prep. Biochem. 11, 413-435), was studied by ultrafiltration in 0.2 M NaCl, pH 5.2 and pH 6.5 to 7, and at 23 to 25 degrees C. The association constant (...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(83)90599-4

    authors： Sophianopoulos JA,Sophianopoulos AJ,MacMahon WC

    更新日期：1983-06-01 00:00:00

  • Roles of xanthophyll carotenoids in protection against photoinhibition and oxidative stress in the cyanobacterium Synechococcus sp. strain PCC 7002.

    abstract：:Synechococcus sp. strain PCC 7002 is a robust, genetically tractable cyanobacterium that produces six different xanthophyll carotenoids (zeaxanthin, cryptoxanthin, myxoxanthophyll (myxol-2'-fucoside), echinenone, 3'-hydroxyechinenone, and synechoxanthin) and tolerates many environmental stresses, including high light ...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2010.07.007

    authors： Zhu Y,Graham JE,Ludwig M,Xiong W,Alvey RM,Shen G,Bryant DA

    更新日期：2010-12-01 00:00:00

  • Cardiac transcription factor Nkx2.5 interacts with p53 and modulates its activity.

    abstract：:Transcription factor Nkx2.5, essential for heart development, regulates cardiomyocyte-specific gene expression through combinatorial interactions with other cardiac-restricted (GATA4 and dHAND) or ubiquitous (p300) transcription regulators. Here we demonstrate that Nkx2.5 and p53 synergistically activate the promoter ...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2015.02.001

    authors： Kojic S,Nestorovic A,Rakicevic L,Protic O,Jasnic-Savovic J,Faulkner G,Radojkovic D

    更新日期：2015-03-01 00:00:00

  • Effect of age and caloric intake on protein oxidation in different brain regions and on behavioral functions of the mouse.

    abstract：:The objective of this study was to determine if oxidative stress/damage is a possible causal factor in the senescence-related loss of brain functions in the mouse. If such a relationship indeed existed, it was expected that oxidative protein damage would increase with age within regions of the brain associated with se...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1996.0380

    authors： Dubey A,Forster MJ,Lal H,Sohal RS

    更新日期：1996-09-01 00:00:00

  • Reaction of ozone with glycophorin in solution and in lipid vesicles.

    abstract：:Glycophorin from human red blood cells was exposed to ozone in aqueous solution. Amino acid analysis of glycophorin exposed to a 10-fold molar excess of ozone showed that the only residue affected was methionine. Both methionine residues of the protein were oxidized to methionine sulfoxide. Exposure of the oxidized pr...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(92)90491-e

    authors： Banerjee SK,Mudd JB

    更新日期：1992-05-15 00:00:00

  • Effects of the semisynthetic bis-indole derivative KAR-2 on store-operated calcium entry in human neutrophils.

    abstract：:We studied the effect of KAR-2 on cytosolic Ca(2+) level in human neutrophils by using a fluorescent dye (Fura-2) trapped in the cells. KAR-2 is a semisynthetic bis-indole derivative that shares vinblastine anti-microtubular properties, but does not share the vinblastine antagonistic effect on calmodulin. Therefore KA...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2013.07.011

    authors： Salerno C,Capuozzo E

    更新日期：2013-09-01 00:00:00

  • The rat enhancer of split- and hairy-related protein-2 gene: hepatic expression, genomic structure, and promoter analysis.

    abstract：:The rat enhancer of split- and hairy-related protein-2 (SHARP-2) is a basic helix-loop-helix transcription factor. The hepatic expression of SHARP-2 mRNA was investigated under various conditions. The level was decreased in the regenerating rat liver and malignant hepatoma cells. In contrast, the expression of SHARP-2...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2003.11.011

    authors： Hirano S,Yamada K,Kawata H,Shou Z,Mizutani T,Shigematsu Y,Mayumi M,Miyamoto K

    更新日期：2004-02-01 00:00:00

  • Rapid turnover and impaired cell-surface expression of the human folate receptor in mouse L(tk-) fibroblasts, a cell line defective in glycosylphosphatidylinositol tail synthesis.

    abstract：:The human folate receptor (hFR) is a plasma membrane protein that is anchored to the membrane via a glycosylphosphatidylinositol (GPI) tail in some cell types. The KB hFR cDNA sequence predicts a hydrophobic, alpha-helical 31-residue carboxyl terminus that is thought to be the signal for cleavage and attachment of the...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1995.1456

    authors： Chung KN,Roberts S,Kim CH,Kirassova M,Trepel J,Elwood PC

    更新日期：1995-09-10 00:00:00

  • Inhibition of human DNA topoisomerase IB by a cyclometalated gold III compound: analysis on the different steps of the enzyme catalytic cycle.

    abstract：:A gold(III) compound [Au(C^N^C)(IMe)]CF(3)SO(3) (Gold III) has been reported to have anticancer properties as it is able to reduce topoisomerase IB activity in vitro and suppress tumor growth in nude mice model. Here we have investigated the mechanism of inhibition of human topoisomerase IB activity by this compound, ...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2011.10.008

    authors： Castelli S,Vassallo O,Katkar P,Che CM,Sun RW,Desideri A

    更新日期：2011-12-15 00:00:00

  • Differential scanning calorimetry as a tool for protein folding and stability.

    abstract：:Differential scanning calorimetry measures the heat capacity of states and the excess heat associated with transitions that can be induced by temperature change. The integral of the excess heat capacity is the enthalpy for this process. Despite this potentially intimidating sounding physical chemistry background, DSC ...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章,评审

    doi：10.1016/j.abb.2012.09.008

    authors： Johnson CM

    更新日期：2013-03-01 00:00:00

  • Peroxidase-catalyzed formation of quercetin quinone methide-glutathione adducts.

    abstract：:The oxidation of quercetin by horseradish peroxidase/H(2)O(2) was studied in the absence but especially also in the presence of glutathione (GSH). HPLC analysis of the reaction products formed in the absence of GSH revealed formation of at least 20 different products, a result in line with other studies reporting the ...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.2000.1832

    authors： Awad HM,Boersma MG,Vervoort J,Rietjens IM

    更新日期：2000-06-15 00:00:00

  • The effect of putative nucleation sites on the loading and stability of iron in ferritin.

    abstract：:The L chain of the iron storage protein ferritin contains more putative nucleation sites in the core (Glu53, 56, 57, 60, and 63) than does the H chain (Glu61, 64, and 67). Recombinant DNA techniques were used to investigate the role of these putative nucleation sites on iron loading by ceruloplasmin and on the stabili...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1997.0523

    authors： Juan SH,Aust SD

    更新日期：1998-02-15 00:00:00

  • Function of several critical amino acids in human pyruvate dehydrogenase revealed by its structure.

    abstract：:Pyruvate dehydrogenase (E1), an alpha(2)beta(2) tetramer, catalyzes the oxidative decarboxylation of pyruvate and reductive acetylation of lipoyl moieties of the dihydrolipoamide acetyltransferase. The roles of betaW135, alphaP188, alphaM181, alphaH15, and alphaR349 of E1 determined by kinetic analysis were reassessed...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2004.06.027

    authors： Korotchkina LG,Ciszak EM,Patel MS

    更新日期：2004-09-15 00:00:00

  • Abnormal membrane properties of the sarcoplasmic reticulum of pigs susceptible to malignant hyperthermia: modes of action of halothane, caffeine, dantrolene, and two other drugs.

    abstract：:The role of sarcoplasmic reticulum (SR) in malignant hyperthermia (MH) was studied using the heavy microsomal fraction prepared from semitendinosus muscles of both normal and genetically MH-susceptible pigs. In the presence of ATP, SR was loaded with 70 nmol Ca2+/mg SR protein. Under these conditions, MH-SR demonstrat...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(86)90587-4

    authors： Ohnishi ST,Waring AJ,Fang SR,Horiuchi K,Flick JL,Sadanaga KK,Ohnishi T

    更新日期：1986-06-01 00:00:00

  • The mechanism of helical transition of proteins by organic solvents.

    abstract：:This paper describes a theory for the mechanism of three-state transition of proteins which is often observed in aqueous organic cosolvent systems, i.e., from the native, via intermediate to helical forms. The first transition, accompanied by changes in the tertiary and/or secondary structures, was explained by larger...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(85)90004-9

    authors： Arakawa T,Goddette D

    更新日期：1985-07-01 00:00:00

  • Diabetes alters LDL receptor and PCSK9 expression in rat liver.

    abstract：:Since the hepatic LDL receptor is regarded as a major determinant of plasma LDL levels, the effect of diabetes on the expression of this receptor was examined in rat liver. Inducing diabetes with streptozotocin caused a significant reduction in hepatic LDL receptor mRNA levels in concert with an increase in serum chol...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2007.11.009

    authors： Niesen M,Bedi M,Lopez D

    更新日期：2008-02-15 00:00:00

  • Rat brain hexokinase: location of the substrate hexose binding site in a structural domain at the C-terminus of the enzyme.

    abstract：:A glucose analog, N-(bromoacetyl)-D-glucosamine (GlcNBrAc), previously used to label the glucose binding sites of rat muscle Type II and bovine brain Type I hexokinases, also inactivates rat brain hexokinase (ATP:D-hexose 6-phosphotransferase, EC 2.7.1.1) with pseudo-first-order kinetics. Inactivation occurs predomina...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(87)90116-0

    authors： Schirch DM,Wilson JE

    更新日期：1987-05-01 00:00:00

  • Human platelet activation is inhibited by the occupancy of glycoprotein IIb/IIIa receptor.

    abstract：:We studied the effect of glycoprotein GPIIb/IIIa (integrin alpha IIb beta 3) receptor occupancy by adenosine 5',1-thiotriphosphate (ATP alpha S), a competitive inhibitor of the ADP receptor, by fibrinogen, and by peptides containing the RGD (Arg-Gly-Asp) sequence as RGDW (Arg-Gly-Asp-Trp), RGDS (Arg-Gly-Asp-Ser), or t...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1996.0408

    authors： Padoin E,Alexandre A,Cavallini L,Polverino de Laureto P,Rao GH,Doni MG

    更新日期：1996-09-15 00:00:00

  • The dihydroorotate dehydrogenases: Past and present.

    abstract：:The flavoenzyme dihydroorotate dehydrogenase catalyzes the stereoselective oxidation of (S)-dihydroorotate to orotate in the fourth of the six conserved enzymatic reactions involved in the de novo pyrimidine biosynthetic pathway. Inhibition of pyrimidine metabolism by selectively targeting DHODHs has been exploited in...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章,评审

    doi：10.1016/j.abb.2017.06.019

    authors： Reis RAG,Calil FA,Feliciano PR,Pinheiro MP,Nonato MC

    更新日期：2017-10-15 00:00:00

  • Monoterpene biosynthesis: mechanistic evaluation of the geranyl pyrophosphate:(-)-endo-fenchol cyclase from fennel (Foeniculum vulgare).

    abstract：:Geranyl pyrophosphate:(-)-endo-fenchol cyclase catalyzes the conversion of geranyl pyrophosphate to (-)-endo-fenchol by a process thought to involve the initial isomerization of the substrate to the tertiary allylic isomer, linalyl pyrophosphate, and the subsequent cyclization of this bound intermediate. Studies with ...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(89)90134-3

    authors： Croteau R,Miyazaki JH,Wheeler CJ

    更新日期：1989-03-01 00:00:00

  • Characterization of the complete genomic structure of human thromboxane synthase gene and functional analysis of its promoter.

    abstract：:Thromboxane A2 (TXA) is a potent vasoconstrictor and mediator of platelet aggregation. Thromboxane synthase (TXAS), which catalyzes the biosynthesis of TXA, is a member of the cytochrome P450 superfamily. We report here the complete genomic structure of the human TXAS gene. The gene contains 13 exons and is 193 kb lon...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1996.0464

    authors： Tazawa R,Green ED,Ohashi K,Wu KK,Wang LH

    更新日期：1996-10-15 00:00:00

  • Frontal gel chromatography of interacting systems: theoretical and experimental evaluation of the shapes of elution profiles for systems of the type A + B in equilibrium C.

    abstract：:A theoretical and experimental study has been made of the advancing elution profile in frontal gel chromatography of interacting systems for which the elution volume of the complex is smaller than that of the larger reactant. First, Gilbert-Jenkins theory is used to delineate the form of the elution profile from the m...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(87)90427-9

    authors： Cann JR,Winzor DJ

    更新日期：1987-07-01 00:00:00

  • Two phosphorylated forms of myosin in thrombin-stimulated platelets.

    abstract：:Three forms of 20-kDa myosin light chain (MLC), unphosphorylated, monophosphorylated, and diphosphorylated MLC (designated 20K, 20K-P, and 20K-PP) were demonstrated in thrombin-stimulated human platelets by two different gel electrophoretic methods: in the presence of glycerol urea or in two dimensions (isoelectric an...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(88)90337-2

    authors： Naka M,Saitoh M,Hidaka H

    更新日期：1988-03-01 00:00:00

  • Site-directed mutagenesis of cation coordinating residues in the gastric H,K-ATPase.

    abstract：:Site-mutations were introduced into putative cation binding site 1 of the H,K-ATPase at glu-797, thr-825, and glu-938. The side chain oxygen of each was not essential but the mutations produced different activation and inhibition kinetics. Site mutations thr-825 (ala, leu) and glu-938 (ala, gln) modestly decreased the...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.2000.2243

    authors： Rulli SJ,Louneva NM,Skripnikova EV,Rabon EC

    更新日期：2001-03-01 00:00:00

  • The mechanisms of reduction of protein mixed disulfides (dethiolation) in cardiac tissue.

    abstract：:Dethiolation of proteins (reduction of protein mixed disulfides) by NADPH-dependent and glutathione (GSH)-dependent enzymes, and by nonenzymatic reaction with GSH, was studied by electrofocusing methodology with glycogen phosphorylase b and creatine kinase as substrates. Phosphorylase b was not rapidly dethiolated by ...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(89)90413-x

    authors： Park EM,Thomas JA

    更新日期：1989-10-01 00:00:00

  • Gene expression of iron-related proteins during iron deficiency caused by scurvy in guinea pigs.

    abstract：:The regulation of expression of hepatic iron-related proteins was examined during iron deficiency caused by scurvy in guinea pigs. Previous studies showed that some effects of scurvy, such as suppression of collagen gene expression, result from events associated with weight loss. During the initial phase of scurvy whe...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1996.0037

    authors： Gosiewska A,Mahmoodian F,Peterkofsky B

    更新日期：1996-01-15 00:00:00

  • Microarray expression profiling and functional characterization of AtTPS genes: duplicated Arabidopsis thaliana sesquiterpene synthase genes At4g13280 and At4g13300 encode root-specific and wound-inducible (Z)-gamma-bisabolene synthases.

    abstract：:The Arabidopsis thaliana genome contains at least 32 terpenoid synthase (AtTPS) genes [Aubourg et al., Mol. Genet. Genom. 267 (2002) 730] a few of which have recently been characterized. Based on hierarchical cluster analysis of AtTPS gene expression, measured by microarray profiling and validated with published expre...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2005.09.019

    authors： Ro DK,Ehlting J,Keeling CI,Lin R,Mattheus N,Bohlmann J

    更新日期：2006-04-15 00:00:00

  • A novel hepatopancreatic phospholipase A2 from Hexaplex trunculus with digestive and toxic activities.

    abstract：:A marine snail digestive phospholipase A2 (mSDPL) was purified from delipidated hepatopancreas. Unlike known digestive phospholipases A2, which are 14 kDa proteins, the purified mSDPL has a molecular mass of about 30 kDa. It has a specific activity of about 180 U/mg measured at 50 degrees C and pH 8.5 using phosphatid...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2009.11.020

    authors： Zarai Z,Bacha AB,Horchani H,Bezzine S,Zouari N,Gargouri Y,Mejdoub H

    更新日期：2010-02-15 00:00:00

  • Modulation of tumor cell stiffness and migration by type IV collagen through direct activation of integrin signaling pathway.

    abstract：:Excessive collagen deposition plays a critical role in tumor progression and metastasis. To understand how type IV collagen affects mechanical stiffness and migration, low-collagen-IV-expressing transfectants of B16F10, U118MG, and Huh7 (denoted shCol cells) were established by the lentiviral-mediated delivery of smal...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2014.05.004

    authors： Chen SY,Lin JS,Yang BC

    更新日期：2014-08-01 00:00:00

  • Upregulation of α2-adrenoceptor synthesis in SHR cardiomyocytes: Recompense without sense - Increased amounts, impaired commands.

    abstract：AIMS:to investigate α2-AR subtype distribution and the relationship between receptor amounts and their functionality in normotensive and spontaneously hypertensive rats. METHODS:experiments were performed on left ventricular cardiomyocytes isolated from Wistar rats and SHR (2-2.5 months). Molecular routine tools (RT-P...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2019.108109

    authors： Kokoz YM,Evdokimovskii EV,Maltsev AV

    更新日期：2019-10-15 00:00:00