Abnormal membrane properties of the sarcoplasmic reticulum of pigs susceptible to malignant hyperthermia: modes of action of halothane, caffeine, dantrolene, and two other drugs.

abstract：:Stem cells maintain their self-renewal and multipotency capacities through a self-organizing network of transcription factors and intracellular pathways activated by extracellular signaling from the microenvironment or "niche" in which they reside in vivo. In the adult mammalian brain new neurons continue to be genera...

journal_title：Archives of biochemistry and biophysics

authors： Porlan E,Perez-Villalba A,Delgado AC,Ferrón SR

更新日期：2013-06-01 00:00:00

abstract：:The formation of alpha-hydroxyethyl radical from ethanol by deermouse microsomes supplemented with NADPH has been demonstrated with the EPR technique of spin trapping with alpha-(4-pyridyl-1-oxide)-N-t-butylnitrone (POBN) as the spin trap, in the presence of deferoxamine mesylate. Superoxide dismutase prevented the fo...

journal_title：Archives of biochemistry and biophysics

authors： Knecht KT,Thurman RG,Mason RP

更新日期：1993-06-01 00:00:00

abstract：:In bovine retinal pigment epithelium membranes we have found three hydrolases which were active against trans-retinyl palmitate. This was possible by assaying different subcellular fractions as a function of pH in the range 3-9. Detection of these activities has been favored by the use in the enzyme assay of Triton X-...

journal_title：Archives of biochemistry and biophysics

authors： Gueli MC,Nicotra CM,Pintaudi AM,Paganini A,Pandolfo L,De Leo G,Di Bella MA

更新日期：1991-08-01 00:00:00

abstract：:Studies have demonstrated that accumulation of mitochondrial tocopheroxyl radical, the primary oxidation product of alpha-tocopherol, accompanies rapid consumption of tocopherol. Enzyme-linked electron flow lowers both the steady-state concentration of the radical and the consumption of tocopherol. Reduction of tocoph...

journal_title：Archives of biochemistry and biophysics

authors： Maguire JJ,Kagan V,Ackrell BA,Serbinova E,Packer L

更新日期：1992-01-01 00:00:00

abstract：:Human alcohol dehydrogenases (ADH1 and ADH4) actively use retinoids oxidized at the cyclohexenyl ring (4-oxo-, 4-hydroxy-, and 3,4-didehydro-retinoids), which are functional compounds in several cells and tissues (i.e., in human skin). Remarkably, activities with 4-oxo-retinal and 4-hydroxy-retinol (kcat = 2050 min(-1...

journal_title：Archives of biochemistry and biophysics

authors： Martras S,Alvarez R,Gallego O,Domínguez M,de Lera AR,Farrés J,Parés X

更新日期：2004-10-15 00:00:00

abstract：:(6R)-5,6,7,8-Tetrahydro-L-biopterin (R-THBP) is a cofactor not only for aromatic amino acid hydroxylases in mammalian tissues but also for nitric oxide synthase (NOS) induced by endotoxins or cytokines in some kinds of cells. Recently it has been reported that nitric oxide (NO) has biological activity in endothelium a...

journal_title：Archives of biochemistry and biophysics

authors： Nunokawa Y,Ishihara T,Kanai T,Noguchi T

更新日期：1992-11-01 00:00:00

abstract：:Although in vitro models are often used in beta-carotene research, knowledge about the uptake and metabolism of beta-carotene in cell lines is lacking. We measured by HPLC the intracellular levels of beta-carotene and its metabolites in 9 human intestinal and lung cell lines after exposure to 1 microM beta-carotene du...

journal_title：Archives of biochemistry and biophysics

authors： Franssen-van Hal NL,Bunschoten JE,Venema DP,Hollman PC,Riss G,Keijer J

更新日期：2005-07-01 00:00:00

abstract：:The parent pyridine nucleotide NAD is the end product of oxidative tryptophan catabolism via the kynurenine pathway. Indoleamine 2, 3-dioxygenase, the rate-limiting enzyme for this pathway, is induced by the proinflammatory cytokine interferon-gamma. The aim of this study was to investigate the effect of interferon-ga...

journal_title：Archives of biochemistry and biophysics

authors： Grant RS,Passey R,Matanovic G,Smythe G,Kapoor V

更新日期：1999-12-01 00:00:00

abstract：:Three major pathways mediate amino acid transport into mammalian cells: the A-system and the ASC-system, which require a sodium gradient across the plasma membrane, and the L-system, which has no requirement for a sodium gradient. We have found that the lymphocytes from patients with B-cell chronic lymphocytic leukemi...

journal_title：Archives of biochemistry and biophysics

authors： Segel GB,Tometsko AM,Lichtman MA

更新日期：1985-11-01 00:00:00

abstract：:Previous studies demonstrated that liver microsomes from untreated rats catalyze the omega, omega-1, and omega-2 hydroxylation of prostaglandins [K. A. Holm, R. J. Engell, and D. Kupfer (1985) Arch. Biochem. Biophys. 237, 477-489]. The current study examined the regioselectivity of hydroxylation of PGE1 and PGE2 by pu...

journal_title：Archives of biochemistry and biophysics

authors： Kupfer D,Jansson I,Favreau LV,Theoharides AD,Schenkman JB

更新日期：1988-02-15 00:00:00

abstract：:The visible and near-uv absorption and circular dichroic spectra were determined for spinach and poplar plastocyanin under a variety of conditions. The visible spectra showed that the copper center was invariant to changes in species, chemical modification with ethylenediamine, and addition of high concentrations of s...

journal_title：Archives of biochemistry and biophysics

authors： Draheim JE,Anderson GP,Pan RL,Rellick LM,Duane JW,Gross EL

更新日期：1985-02-15 00:00:00

abstract：:The pH dependence of the activity of Cu,Zn superoxide dismutases from bovine erythrocytes and shark liver was studied by pulse radiolysis in both the native enzymes and those chemically modified at lysine side chains. The study was aimed at identifying the residues responsible for the activity decrease at pH > 9, obse...

journal_title：Archives of biochemistry and biophysics

authors： Polticelli F,O'Neill P,Costanzo S,Lania A,Rotilio G,Desideri A

更新日期：1995-08-01 00:00:00

abstract：:To study the relationship between neutral aminopeptidase activity and hemoglobin accumulation in malaria parasites, we treated mice infected with Plasmodium berghei NYU-2 with chloroquine intraperitoneally in doses ranging from 0.3 to 3 micromol per 25 g mouse. Preparations of infected erythrocytes (normalized to repr...

journal_title：Archives of biochemistry and biophysics

authors： Fitch CD,Cai GZ,Chen YF,Ryerse JS

更新日期：2003-02-15 00:00:00

abstract：:We tested the effects of a series of synthetic oligonucleotides (hybridons) complementary to the 5' noncoding and coding regions of rabbit beta-globin mRNA on endogenous protein synthesis in a rabbit reticulocyte cell-free translation system. With highly purified hybridons inhibition was completely specific for beta-g...

journal_title：Archives of biochemistry and biophysics

authors： Goodchild J,Carroll E 3rd,Greenberg JR

更新日期：1988-06-01 00:00:00

abstract：:Plant 3-hydroxy-3-methylglutaryl-CoA reductase(HMGR; EC 1.1.1.34) and sucrose-phosphate synthase (SPS; EC 2.4.1.14) and synthetic peptides designed from the known phosphorylation sites of plant HMGR (SAMS*: KSHMKYNRSTKDVK), rat acetyl-CoA carboxylase (SAMS: HMRSAMSGLHLVKRR), spinach SPS (SP2: GRRJRRISSVEJJDKK), and sp...

journal_title：Archives of biochemistry and biophysics

authors： Toroser D,Huber SC

更新日期：1998-07-15 00:00:00

abstract：:The interaction of 5'-deoxy-5'-thioadenosine 5'-monophosphate (A(S)MP) and 5'-deoxy-5'-thioinosine 5'-monophosphate (I(S)MP) with snake venom, 5'nucleotidase, and calf intestinal mucosa alkaline phosphatase has been characterized. The substrates, A(S)MP and I(S)MP, are analogs of adenosine 5'-monophosphate and inosine...

journal_title：Archives of biochemistry and biophysics

authors： Rossomando EF,Cordis GA,Markham GD

更新日期：1983-01-01 00:00:00

abstract：:Dideoxyosones (DDOs) are intermediates in the synthesis of advanced glycation endproducts (AGEs), such as pentosidine and glucosepane. Although the formation of pentosidine and glucosepane in the human lens has been firmly established, the formation of DDOs has not been demonstrated. The aim of this study was to devel...

journal_title：Archives of biochemistry and biophysics

authors： Linetsky M,Kaid Johar SR,Meltretter J,Padmanabha S,Parmar T,Vasavada AR,Pischetsrieder M,Nagaraj RH

更新日期：2011-10-01 00:00:00

abstract：:We recently cloned a rat brain agmatinase-like protein (ALP) whose amino acid sequence greatly differs from other agmatinases and exhibits a LIM-like domain close to its carboxyl terminus. The protein was immunohistochemically detected in the hypothalamic region and hippocampal astrocytes and neurons. We now show that...

journal_title：Archives of biochemistry and biophysics

authors： Castro V,Fuentealba P,Henríquez A,Vallejos A,Benítez J,Lobos M,Díaz B,Carvajal N,Uribe E

更新日期：2011-08-01 00:00:00

abstract：:Fluidity of the red blood cell membrane decreases as pH changes from 8 to 7.5. In rat liver mitochondrial (RLM) membrane fluidity precipitously declines as pH drops from 7.35 toward 7.0. With dithionitrobenzoate (Nbs2), reaction rates of mitochondrial -SH groups from rat liver and heart (RHM) and in beef heart submito...

journal_title：Archives of biochemistry and biophysics

authors： Zimmer G,Freisleben HJ,Fuchs J

更新日期：1990-11-01 00:00:00

abstract：:The essential Zn(II) in bovine liver dihydropyrimidine amidohydrolase (DHPase) was removed by incubation with 2,6-dipicolinic acid and replaced with Mn(II). Electron paramagnetic resonance studies of Mn(II) binding show that there are four binding sites per tetramer, and the dissociation constant at pH 7.5 is 13.5 mic...

journal_title：Archives of biochemistry and biophysics

authors： Lee MH,Pettigrew DW,Sander EG,Nowak T

更新日期：1987-12-01 00:00:00

abstract：:Using proton NMR, the solution conformation of the A ring of vitamin D3 and its analogs has been studied by application of the Karplus relation to the observed coupling constants. The A-ring conformations of vitamins D3, D2, and 25-hydroxyvitamin D3 were found to be solvent dependent, with a clear preference for an eq...

journal_title：Archives of biochemistry and biophysics

authors： Helmer B,Schnoes HK,DeLuca HF

更新日期：1985-09-01 00:00:00

abstract：:Biotin synthase catalyzes the insertion of a sulfur atom between two carbon atoms of dethiobiotin to form biotin in the last step of the biotin biosynthesis pathway. In Escherichia coli, biotin synthase is coded for by bioB gene. We report here cloning, sequencing, and initial functional characterization of the yeast ...

journal_title：Archives of biochemistry and biophysics

authors： Zhang S,Sanyal I,Bulboaca GH,Rich A,Flint DH

更新日期：1994-02-15 00:00:00

abstract：:Novel vitamin D3 derivatives, 26-homo-delta 22-1 alpha,25(S)-dihydroxyvitamin D3 and 26-homo-delta 22-1 alpha,25(R)-dihydroxyvitamin D3 were compared with the native hormone, 1,25-dihydroxyvitamin D3, and with other vitamin D3 derivatives, in inhibition of cell growth, induction of phenotypic differentiation, and c-my...

journal_title：Archives of biochemistry and biophysics

authors： Koizumi T,Nakao Y,Ishizuka S,Oshida J,Hara N,Ikekawa N,Fijita T

更新日期：1990-02-01 00:00:00

abstract：:Interactions between loops 2, 2' and 4, known as the loop bundle, stabilize the active site of caspase-3. Loop 4 (L4) is of particular interest due to its location between the active site and the dimer interface. We have disrupted a salt bridge between K242 and E246 at the base of L4 to determine its role in overall c...

journal_title：Archives of biochemistry and biophysics

authors： Walters J,Swartz P,Mattos C,Clark AC

更新日期：2011-04-01 00:00:00

abstract：:Aspartate aminotransferase (AspAT) isozymes from Eleusine coracana (an NAD-malic enzyme type C4 plant) were examined. Three groups of isoenzymes were identified (AspAT-1, AspAT-2, and AspAT-3). AspAT-1 (localized in the mesophyll cells) and AspAT-3 (localized in the bundle sheath cells), both of which are considered t...

journal_title：Archives of biochemistry and biophysics

authors： Taniguchi M,Sugiyama T

更新日期：1990-11-01 00:00:00

abstract：:Phosphofructokinase was purified from muscle tissue of two different molluscs, edible snails, Helix pomatia (gastropoda), and mussels, Mytilus edulis (bivalvia). Under denaturing conditions, both enzymes had a molecular mass of 82 kDa. In the presence of ATP-Mg2+, the enzymes were rapidly phosphorylated in vitro by th...

journal_title：Archives of biochemistry and biophysics

authors： Biethinger M,Hoffmann R,Hofer HW

更新日期：1991-06-01 00:00:00

abstract：:Two new types of bacterial Fe-S L-serine dehydratases have been identified. These join two previously recognized enzyme types, for a total of four, that are distinguished on the basis of domain arrangement and amino acid sequence. A Type 3 enzyme from Amphibacillus xylanus (axLSD) and a Type 4 enzyme from Heliscomenob...

journal_title：Archives of biochemistry and biophysics

authors： Xu XL,Grant GA

更新日期：2013-12-01 00:00:00

abstract：:A new galactose-specific lectin has been purified from the extracts of Trichosanthes dioica seeds by affinity chromatography on cross-linked guar gum. The purified lectin (T. dioica seed lectin, TDSL) moved as a single symmetrical peak on gel filtration on Superose-12 in the presence of 0.1 M lactose with an M(r) of 5...

journal_title：Archives of biochemistry and biophysics

authors： Sultan NA,Kenoth R,Swamy MJ

更新日期：2004-12-15 00:00:00

abstract：:A detailed knowledge about the substrate specificities of the heparin lyases is necessary when using these enzymes as tools for elucidating the sequence of heparin and heparan sulfate. The substrate specificity of heparin lyases I, II, and III have been profiled with structurally defined, heparin-derived oligosacchari...

journal_title：Archives of biochemistry and biophysics

authors： Desai UR,Wang HM,Linhardt RJ

更新日期：1993-11-01 00:00:00

abstract：:Vacuolar H(+)-translocating inorganic pyrophosphatase (V-PPase; EC 3.6.1.1) is a homodimeric proton-translocase; it contains a single type of polypeptide of approximately 81kDa. A line of evidence demonstrated that the carboxyl terminus of V-PPase is relatively conserved in various plant V-PPases and presumably locate...

journal_title：Archives of biochemistry and biophysics

authors： Lin HH,Pan YJ,Hsu SH,Van RC,Hsiao YY,Chen JH,Pan RL

更新日期：2005-10-15 00:00:00