Abstract：
:Dideoxyosones (DDOs) are intermediates in the synthesis of advanced glycation endproducts (AGEs), such as pentosidine and glucosepane. Although the formation of pentosidine and glucosepane in the human lens has been firmly established, the formation of DDOs has not been demonstrated. The aim of this study was to develop a reliable method to detect DDOs in lens proteins. A specific DDO trapping agent, biotinyl-diaminobenzene (3,4-diamino-N-(3-[5-(2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoyl]aminopropyl)benzamide) (BDAB) was added during in vitro protein glycation or during protein extraction from human lenses. In vitro glycated human lens protein showed strong reaction in monomeric and polymeric crosslinked proteins by Western blot and ELISA. Glycation of BSA in the presence of BDAB resulted in covalent binding of BDAB to the protein and inhibited pentosidine formation. Mass spectrometric analysis of lysozyme glycated in the presence of BDAB showed the presence of quinoxalines at lysine residues at positions K1, K33, K96, and K116. The ELISA results indicated that cataractous lens proteins contain significantly higher levels of DDO than non-cataractous lenses (101.9±67.8 vs. 31.7±19.5AU/mg protein, p<0.0001). This study provides first direct evidence of DDO presence in human tissue proteins and establishes that AGE crosslink synthesis in the human lens occurs via DDO intermediates.
journal_name
Arch Biochem Biophysjournal_title
Archives of biochemistry and biophysicsauthors
Linetsky M,Kaid Johar SR,Meltretter J,Padmanabha S,Parmar T,Vasavada AR,Pischetsrieder M,Nagaraj RHdoi
10.1016/j.abb.2011.07.012subject
Has Abstractpub_date
2011-10-01 00:00:00pages
16-26issue
1-2eissn
0003-9861issn
1096-0384pii
S0003-9861(11)00264-5journal_volume
514pub_type
杂志文章abstract：:Altered iso-1- and iso-2-cytochromes c, with certain amino acid replacements, occur at diminished levels due to degradation in the yeast Saccharomyces cerevisiae. A subclass of the labile isocytochromes c are significantly protected from degradation by the presence of cytochromes a.a3 and c1, the physiological partner...
journal_title：Archives of biochemistry and biophysics
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pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：1996-12-01 00:00:00
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更新日期：2014-07-15 00:00:00
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pub_type： 杂志文章
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更新日期：1994-02-15 00:00:00
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journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：1987-05-01 00:00:00
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pub_type： 杂志文章
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更新日期：1984-04-01 00:00:00
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journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
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更新日期：1998-05-15 00:00:00
