Hormone-induced phosphorylation of the plasma membrane calcium pump in cultured aortic endothelial cells.

Abstract:

:The regulation of the plasma membrane Ca2+ pump by hormones via phosphorylation in intact cells has not been clearly established. We now present evidence that the Ca2+ pump is phosphorylated on both serine and threonine residues in unstimulated and stimulated cultured rat aortic endothelial cells. Among the stimuli tested, the protein kinase C activator phorbol 12-myristate 13-acetate (PMA) was most potent and increased the level of phosphorylation threefold, while the cAMP-dependent protein kinase activator 8-(4-chlorophenylthio)-cAMP (CPT-cAMP) stimulated the phosphorylation 1.6-fold. Two-dimensional tryptic phosphopeptide maps of the Ca2+ pump from unstimulated and CPT-cAMP-stimulated cells have identical patterns (five phosphopeptides) while PMA-stimulated cells have three additional phosphopeptides. Isoproterenol-, ATP-, angiotensin II-, and bradykinin-stimulated cells also have increased levels of Ca2+ pump phosphorylation. Stimuli-induced phosphorylation of the Ca2+ pump was rapid (5-10 min) and was concomitant with stimulated calcium efflux from the same cells. This is the first direct evidence that the plasma membrane Ca2+ pump in intact cells is regulated by various hormones or agonists via cAMP-dependent protein kinase or protein kinase C phosphorylation.

journal_name

Arch Biochem Biophys

authors

Wang KK,Du YS,Diglio C,Tsang W,Kuo TH

doi

10.1016/0003-9861(91)90448-r

subject

Has Abstract

pub_date

1991-08-15 00:00:00

pages

103-8

issue

1

eissn

0003-9861

issn

1096-0384

pii

0003-9861(91)90448-R

journal_volume

289

pub_type

杂志文章
  • Cyanidin-3-O-glucoside functions like chemical chaperone and attenuates the glycation mediated amyloid formation in albumin.

    abstract::In this study, chemical chaperone like function of cyanidin-3-O-glucoside (C3G) was investigated through fluorescence spectroscopy, UV-visible spectroscopy, circular dichroism spectroscopy, confocal microscopy, scanning electron microscopy and molecular docking studies. Early and advanced glycation inhibitory effect w...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/j.abb.2018.02.012

    authors: Prasanna G,Jing P

    更新日期:2018-04-02 00:00:00

  • Glycosphingolipid composition of human semen.

    abstract::Glycosphingolipids were extracted from human semen and purified. Based on the fluorometric assay of sphingosine, in spermatozoa a content of 4.4 +/- 0.9 nmol/10(8) cells of gangliosides and 22.1 +/- 1.7 nmol/10(8) cells of neutral glycosphingolipids was determined. Seminal plasma contained 4.1 +/- 0.6 nmol ganglioside...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/0003-9861(87)90579-0

    authors: Ritter G,Krause W,Geyer R,Stirm S,Wiegandt H

    更新日期:1987-09-01 00:00:00

  • Long non-coding RNA ROR promotes radioresistance in hepatocelluar carcinoma cells by acting as a ceRNA for microRNA-145 to regulate RAD18 expression.

    abstract::Radiotherapy plays a limited role in the treatment of hepatocellular carcinoma (HCC) due to the development of resistance. Therefore, further investigation of underlying mechanisms involved in HCC radioresistance is warranted. Increasing evidence shows that long non-coding RNAs (linc-RNAs) are involved in the patholog...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/j.abb.2018.03.018

    authors: Chen Y,Shen Z,Zhi Y,Zhou H,Zhang K,Wang T,Feng B,Chen Y,Song H,Wang R,Chu X

    更新日期:2018-05-01 00:00:00

  • Role of vitamin E in ascorbate-dependent protein thiol oxidation in rat liver endoplasmic reticulum.

    abstract::Addition of ascorbate or its generation from gulonolactone causes the oxidation of protein thiols and a simultaneous dehydroascorbate formation in rat liver microsomes. The participation of vitamin E in the phenomenon was studied. We measured ascorbate and protein thiol oxidation and lipid peroxidation in vitamin E de...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1006/abbi.2000.2260

    authors: Csala M,Szarka A,Margittai E,Mile V,Kardon T,Braun L,Mandl J,Bánhegyi G

    更新日期:2001-04-01 00:00:00

  • Effect of the phosphate substrate on drug-inhibitor binding to human purine nucleoside phosphorylase.

    abstract::The thermodynamics of the drug-inhibitors acyclovir, ganciclovir, and 9-benzylguanine binding to human purine nucleoside phosphorylase (hsPNP) were determined from isothermal titration calorimetry as a function of the substrate phosphate ion (Pi) concentration from 0 to 0.125 M and temperature from 15 degrees C to 35 ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/j.abb.2008.10.008

    authors: Todorova NA,Schwarz FP

    更新日期:2008-12-15 00:00:00

  • Interflavin one-electron transfer in the inducible nitric oxide synthase reductase domain and NADPH-cytochrome P450 reductase.

    abstract::In this study, we have analyzed interflavin electron transfer reactions from FAD to FMN in both the full-length inducible nitric oxide synthase (iNOS) and its reductase domain. Comparison is made with the interflavin electron transfer in NADPH-cytochrome P450 reductase (CPR). For the analysis of interflavin electron t...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/j.abb.2005.05.027

    authors: Yamamoto K,Kimura S,Shiro Y,Iyanagi T

    更新日期:2005-08-01 00:00:00

  • Protein engineering of thromboxane synthase: conversion of membrane-bound to soluble form.

    abstract::Thromboxane A2 synthase (TXAS) binds to the endoplasmic reticulum membrane and catalyzes both an isomerization of prostaglandin H2 (PGH2) to form thromboxane A2 (TXA2) and a fragmentation of PGH2 to form 12-hydroxy-5,8,10-heptadecatrienoic acid (HHT) and malondialdehyde (MDA). TXAS is a non-classic cytochrome P450 in ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/s0003-9861(03)00287-x

    authors: Hsu PY,Wang LH

    更新日期:2003-08-01 00:00:00

  • The purification of alpha 1-antichymotrypsin from human serum using DNA-cellulose chromatography.

    abstract::By exploiting its capacity for binding to DNA, the protease inhibitor alpha 1-antichymotrypsin has been isolated from human serum by ammonium sulfate fractionation and successive chromatography on QAE-Sephadex, DNA-cellulose, and Sephacryl S-300. This experimental procedure compares favorably with existing methods for...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/0003-9861(83)90034-6

    authors: Abdullah M,Siddiqui AA,Hill JA,Davies RJ

    更新日期:1983-08-01 00:00:00

  • Glutamine transport in normal and acidotic rat kidney mitochondria.

    abstract::Glutamine transport in both normal and acidotic rat kidney mitochondria was investigated using both isotopic techniques and by spectroscopic measurements in which glutamine metabolism was allowed to occur. Widely used criteria for demonstrating the occurrence of carrier-mediated transport were successfully applied in ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1006/abbi.1994.1513

    authors: Atlante A,Passarella S,Minervini GM,Quagliariello E

    更新日期:1994-12-01 00:00:00

  • Regulation of 25-hydroxyvitamin D3 1alpha-hydroxylase gene expression by parathyroid hormone and 1,25-dihydroxyvitamin D3.

    abstract::The conversion of 25-hydroxyvitamin D3 to 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3) takes place mainly in the kidney and is catalyzed by the enzyme 1alpha-hydroxylase. Parathyroid hormone (PTH) and 1,25-(OH)2D3 are well-known regulators of this tightly controlled step, but the mechanisms by which they modulate 1alpha-hy...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1006/abbi.2000.1970

    authors: Brenza HL,DeLuca HF

    更新日期:2000-09-01 00:00:00

  • Regulation and intracellular localization of the biotin holocarboxylase synthetase of 3T3-L1 cells.

    abstract::A quantitative assay has been developed to measure holocarboxylase synthetase activity in cellular extracts. This assay was based on measuring the incorporation of [3H]biotin of high specific activity (4.3 Ci/mmol) into purified rat liver apopyruvate carboxylase. With this assay, holocarboxylase synthetase in 3T3-L1 m...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/0003-9861(83)90026-7

    authors: Chang HI,Cohen ND

    更新日期:1983-08-01 00:00:00

  • Cobalamin-dependent dehydratases and a deaminase: radical catalysis and reactivating chaperones.

    abstract::Adenosylcobalamin, a coenzyme form of vitamin B12, is an organometallic compound that participates in about ten enzymatic reactions. These enzymes catalyze chemically challenging reactions by using a highly reactive primary carbon radical that is derived from homolysis of the coenzyme Co-C bond. Among them, diol dehyd...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章,评审

    doi:10.1016/j.abb.2013.11.002

    authors: Toraya T

    更新日期:2014-02-15 00:00:00

  • Bovine liver dihydropyrimidine amidohydrolase: pH dependencies of the steady-state kinetic and proton relaxation rate properties of the Mn(II)-containing enzyme.

    abstract::The essential Zn(II) in bovine liver dihydropyrimidine amidohydrolase (DHPase) was removed by incubation with 2,6-dipicolinic acid and replaced with Mn(II). Electron paramagnetic resonance studies of Mn(II) binding show that there are four binding sites per tetramer, and the dissociation constant at pH 7.5 is 13.5 mic...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/0003-9861(87)90526-1

    authors: Lee MH,Pettigrew DW,Sander EG,Nowak T

    更新日期:1987-12-01 00:00:00

  • Inhibitory effects of streptozotocin, tumor necrosis factor-alpha, and interleukin-1beta on glucokinase activity in pancreatic islets and gene expression of GLUT2 and glucokinase.

    abstract::Treatment of streptozotocin (ST), tumor necrosis factor-alpha (TNF-alpha), and interleukin-1beta (IL-1beta) resulted in destroying insulin-secreting beta-cells of pancreatic islets and impairment of islet glucose oxidation and glucose-induced insulin secretion. IL-1beta and TNF-alpha inhibited insulin release and gluc...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1006/abbi.1998.1004

    authors: Park C,Kim JR,Shim JK,Kang BS,Park YG,Nam KS,Lee YC,Kim CH

    更新日期:1999-02-15 00:00:00

  • Analysis of regulatory mechanisms of an insulin-inducible SHARP-2 gene by (S)-Equol.

    abstract::Small compounds that activate the insulin-dependent signaling pathway have potential therapeutic applications in controlling type 2 diabetes mellitus. The rat enhancer of split- and hairy-related protein-2 (SHARP-2) is an insulin-inducible transcription factor that decreases expression of the phosphoenolpyruvate carbo...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/j.abb.2012.05.026

    authors: Haneishi A,Takagi K,Asano K,Yamamoto T,Tanaka T,Nakamura S,Noguchi T,Yamada K

    更新日期:2012-09-01 00:00:00

  • Metabolism of monoepoxides of methyl linoleate: bioactivation and detoxification.

    abstract::Leukotoxin (ltx) and isoleukotoxin (iltx) methyl esters, are metabolites of methyl linoleic acid, an essential fatty acid. They have been associated with acute respiratory distress syndrome. The observed toxicity of ltx and iltx is, in fact, due to the metabolism of the epoxides to their corresponding diols by soluble...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1006/abbi.2000.1753

    authors: Greene JF,Williamson KC,Newman JW,Morisseau C,Hammock BD

    更新日期:2000-04-15 00:00:00

  • Recognition of type 1 chain oligosaccharides and lacto-series glycolipids by an antibody to human secretory component.

    abstract::Binding of the mouse IgM antibody 6C4 is lost after treatment of human free secretory component with peptide N-glycosidase F (Bakos et al. (1991) J. Immunol. 146, 162-168) or periodate, suggesting that asparagine-linked oligosaccharides contain the epitope recognized by this antibody. Inhibition of antibody binding to...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1006/abbi.1995.1467

    authors: Yu H,Sipes JM,Cashel J,Bakos MA,Goldblum RM,Roberts DD

    更新日期:1995-10-01 00:00:00

  • The conformational analysis of oligosaccharides by H-NMR and HSEA calculation.

    abstract::The application of 1H-nuclear Overhauser enhancement, 1H-spin-lattice-relaxation-time and 1H-chemical shift measurements for the assessment of the conformational preferences of oligosaccharides are briefly reviewed. It is demonstrated that additivity rules, for the correlation of the chemical shifts of similar hydroge...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/0003-9861(83)90128-5

    authors: Lemieux RU,Bock K

    更新日期:1983-02-15 00:00:00

  • Human erythrocyte pyrimidine 5'-nucleotidase, PN-I.

    abstract::Erythrocyte maturation is accompanied by RNA degradation and release of mononucleotides. Pyrimidine 5'-nucleotidase, PN-I, has been purified and characterized. The molecular and enzymatic properties determined for the enzyme shows a 36-kDa and 5.1 pI monomeric protein with no disulfide bridges and no phosphate content...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1006/abbi.2001.2676

    authors: Amici A,Magni G

    更新日期:2002-01-15 00:00:00

  • Effects of nitroxide stable radicals on juglone cytotoxicity.

    abstract::Nitroxides stable radicals are unreactive toward most diamagnetic molecules, but readily undergo one-electron redox reactions with paramagnetic species such as free radicals and transition metals, thus serving as cell-permeable antioxidants. The cytotoxicity of juglone (5-hydroxy-1,4-naphthoquinone), like that of othe...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1006/abbi.1994.1323

    authors: Zhang R,Hirsch O,Mohsen M,Samuni A

    更新日期:1994-08-01 00:00:00

  • Transient adaptation to oxidative stress in yeast.

    abstract::Adaptive responses to the oxidative stress of hydrogen peroxide (H2O2) were studied in the yeast Saccharomyces cerevisciae strain RZ53. Our results show that the growth of naive cells is readily arrested by H2O2 challenge. In contrast, cells that have been preexposed to relatively low H2O2 priming treatments (i.e., ce...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1006/abbi.1995.1128

    authors: Davies JM,Lowry CV,Davies KJ

    更新日期:1995-02-20 00:00:00

  • A novel acetyl-CoA carboxylase inhibitor reduces de novo fatty acid synthesis in HepG2 cells and rat primary hepatocytes.

    abstract::To identify the novel inhibitor of de novo lipogenesis in hepatocytes, we screened for inhibitory activity of triglyceride (TG) synthesis using [14C]acetate in the human hepatoma cell line, HepG2. Using this assay system we discovered the novel compound, benzofuranyl alpha-pyrone (TEI-B00422). TEI-B00422 also inhibite...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/j.abb.2007.09.012

    authors: Sugimoto Y,Naniwa Y,Nakamura T,Kato H,Yamamoto M,Tanabe H,Inoue K,Imaizumi A

    更新日期:2007-12-01 00:00:00

  • ATP, histidine or magnesium ions can protect DNA against sisomicin-induced damage, following stray Cu(II) binding.

    abstract::The oxidative DNA damage by the cupric complexes of sisomicin was investigated in the presence of varying amounts of histidine, ATP, Mg(II) ions or phosphates. We found that by very low concentrations, the amino acid is able to inhibit the cleavage totally. This occurs both by its competition with antibiotic for coppe...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/j.abb.2004.07.018

    authors: Szczepanik W,Swiatek M,Skała J,Jezowska-Bojczuk M

    更新日期:2004-11-01 00:00:00

  • How iron is handled in the course of heme catabolism: Integration of heme oxygenase with intracellular iron transport mechanisms mediated by poly (rC)-binding protein-2.

    abstract::Heme and iron are essential to almost all forms of life. The strict maintenance of heme and iron homeostasis is essential to prevent cellular toxicity and the existence of systemic and intracellular regulation is fundamental. Cytosolic heme can be catabolized and detoxified by heme oxygenases (HOs). Interestingly, fre...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章,评审

    doi:10.1016/j.abb.2019.108071

    authors: Yanatori I,Richardson DR,Toyokuni S,Kishi F

    更新日期:2019-09-15 00:00:00

  • Purification and characterization of an abscisic acid-inducible anionic peroxidase associated with suberization in potato (Solanum tuberosum).

    abstract::An anionic peroxidase (EC 1.11.1.7), thought to be involved in suberization, was purified 110-fold from wound-healing slices of Solanum tuberosum by a combination of ammonium sulfate fractionation, Sephadex G-100 gel filtration, isoelectric focusing, and phenyl-Sepharose CL-4B chromatography in 24% yield. The purified...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/0003-9861(85)90060-8

    authors: Espelie KE,Kolattukudy PE

    更新日期:1985-08-01 00:00:00

  • Purification and some properties of delta 2-isopentenylpyrophosphate:5'AMP delta 2-isopentenyltransferase from the cellular slime mold Dictyostelium discoideum.

    abstract::delta 2-Isopentenylpyrophosphate:5'AMP delta 2-isopentenyltransferase, which catalyzes the formation of isopentenyl-AMP from delta 2-isopentenylpyrophosphate and 5'AMP, was purified 6800-fold from the fruiting body of the cellular slime mold Dictyostelium discoideum using several separation procedures including 5'AMPo...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/0003-9861(84)90446-6

    authors: Ihara M,Taya Y,Nishimura S,Tanaka Y

    更新日期:1984-05-01 00:00:00

  • CK2-mediated phosphorylation of a type II regulatory subunit of cAMP-dependent protein kinase from the mollusk Mytilus galloprovincialis.

    abstract::Two isoforms of regulatory (R) subunit of cAMP-dependent protein kinase (PKA), named R(myt1) and R(myt2), were identified so far in the sea mussel Mytilus galloprovincialis. Out of them, only R(myt2) was phosphorylated in vitro by casein kinase 2 (CK2) using GTP as phosphate donor. CK2 catalytic subunit (CK2alpha) its...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/j.abb.2007.02.008

    authors: Bardales JR,Hellman U,Villamarín JA

    更新日期:2007-05-01 00:00:00

  • Detection and isolation of oligosaccharides with Lea and Leb blood group activities by affinity chromatography using monoclonal antibodies.

    abstract::Affinity columns prepared by immobilizing monoclonal antibodies that specifically recognize the Lea or the Leb blood group antigens can be used for analytical or preparative isolation of oligosaccharides with the corresponding reactivities. The number of immobilized functional antibody combining sites on a column and ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/0003-9861(88)90586-3

    authors: Dakour J,Lundblad A,Zopf D

    更新日期:1988-07-01 00:00:00

  • P8 deficiency increases cellular ROS and induces HO-1.

    abstract::The gene p8 encodes for a small cytoprotective protein with no apparent enzymatic activity being proposed to act as co-transcription factor whose expression is increased during inflammation. Recent data from astrocytes demonstrates that p8 suppression leads to induction of heme oxygenase 1 (HO-1). Here, we assessed th...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/j.abb.2014.11.007

    authors: Weis S,Bielow T,Sommerer I,Iovanna J,Malicet C,Mössner J,Hoffmeister A

    更新日期:2015-01-01 00:00:00

  • Stimulation of bovine brain phospholipase C activity by myelin basic protein requires arginyl residues in peptide linkage.

    abstract::We reported previously a highly purified phosphatidylinositol-specific phospholipase C (PI-PLC) from bovine brain and from human myelin which was stimulated by myelin basic protein. In this paper we report that the stimulation of the PI-PLC activity by myelin basic protein (MBP) requires arginine residues in peptide l...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1006/abbi.1993.1242

    authors: Tompkins TA,Moscarello MA

    更新日期:1993-05-01 00:00:00