Purification, characterization, and subcellular localization of an acid phosphatase from black mustard cell-suspension cultures: comparison with phosphoenolpyruvate phosphatase.


:An acid phosphatase from Brassica nigra (black mustard) leaf petiole cell-suspension cultures has been purified 1633-fold to a final specific activity of 1225 (mumols orthophosphate produced/min)/mg protein and near homogeneity. The native protein was a glycosylated monomer having a molecular mass of 60 kDa and a pI of 4.5. The enzyme displayed a broad pH optimum of about pH 5.6 and was heat stable. The final preparation hydrolyzed a wide variety of phosphate esters. The highest specificity constants were obtained with 3-phosphoglycerate, 2,3-diphosphoglycerate, PPi, and phosphoenolpyruvate (PEP). The enzyme was activated 1.4-fold by 4 mM Mg2+ or Mn2+, but was strongly inhibited by Mo, Pi, F, and several phosphorylated compounds. Subcellular localization experiments revealed that this nonspecific acid phosphatase is probably a secreted enzyme, localized in the cell wall. By contrast, B. nigra PEP phosphatase appeared to be localized in the cell vacuole. Peptide mapping via CNBr fragmentation was employed to investigate the structural relatedness of the two phosphatases. Their respective CNBr cleavage patterns were dissimilar, suggesting that B. nigra acid and PEP phosphatases are distinct polypeptides. Putative metabolic functions of these two phosphatases are discussed in relation to the biochemical adaptations of B. nigra cell-suspension cultures to nutritional phosphate deprivation.


Arch Biochem Biophys


Duff SM,Lefebvre DD,Plaxton WC




Has Abstract


1991-04-01 00:00:00














  • Quantification of cysteine sulfinic acid decarboxylase in male and female rats: effect of adrenalectomy and methionine.

    abstract::Hepatic cysteine sulfinic acid decarboxylase (EC activity has been reported to decrease in response to both L-methionine (Met) feeding and adrenalectomy in rats. A series of experiments was conducted to (a) determine if CSAD depression was evident in female rats fed a methionine-supplemented diet; and (b) de...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Jerkins AA,Steele RD

    更新日期:1992-05-01 00:00:00

  • Calcium desensitizer catechin reverses diastolic dysfunction in mice with restrictive cardiomyopathy.

    abstract::Diastolic dysfunction refers to an impaired relaxation and an abnormality in ventricular blood filling during diastole while systolic function is preserved. Cardiac myofibril hypersensitivity to Ca(2+) is a major factor that causes impaired relaxation of myocardial cells. The present study investigates the effect of t...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Zhang L,Nan C,Chen Y,Tian J,Jean-Charles PY,Getfield C,Wang X,Huang X

    更新日期:2015-05-01 00:00:00

  • Taurine bromamine: a potent oxidant of tryptophan residues in albumin.

    abstract::Taurine is the most abundant free amino acid in leukocytes and can react with HOBr to produce taurine bromamine (Tau-NHBr). The aim of this study was to assess the ability of Tau-NHBr to oxidize tryptophan, either free or as a residue in albumin. We have demonstrated that Tau-NHBr is a powerful oxidant for tryptophan....

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Ximenes VF,da Fonseca LM,de Almeida AC

    更新日期:2011-03-15 00:00:00

  • Dietary control and tissue specific expression of branched-chain alpha-ketoacid dehydrogenase kinase.

    abstract::The branched-chain alpha-ketoacid dehydrogenase complex, catalyst for the rate-limiting step of branched-chain amino acid catabolism, is controlled by a highly specific protein kinase (branched-chain alpha-ketoacid dehydrogenase kinase) that associates tightly with the complex. The activity state (proportion of the en...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Popov KM,Zhao Y,Shimomura Y,Jaskiewicz J,Kedishvili NY,Irwin J,Goodwin GW,Harris RA

    更新日期:1995-01-10 00:00:00

  • Plasma urolithin metabolites correlate with improvements in endothelial function after red raspberry consumption: A double-blind randomized controlled trial.

    abstract::Raspberries are a rich source of ellagitannins and anthocyanins. The aim of this work was to investigate whether raspberry consumption can improve vascular function and to understand which phenolic metabolites may be responsible for the effects. A 3 arm double-blind randomized controlled crossover human intervention t...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章,随机对照试验


    authors: Istas G,Feliciano RP,Weber T,Garcia-Villalba R,Tomas-Barberan F,Heiss C,Rodriguez-Mateos A

    更新日期:2018-08-01 00:00:00

  • Analysis of the stability and function of nucleoplasmin through cysteine mutants.

    abstract::Xenopus laevis nucleoplasmin is a pentameric nuclear chaperone. The relation between the structure and the multifunctional aspects of the molecule has not yet been clearly established. In the course of analysing a C-terminally His-tagged recombinant version of the region equivalent to the trypsin resistant core (r-NP1...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Arnan C,Prieto C,Chiva M,Salvany L,Ausió J,Subirana JA,Saperas N

    更新日期:2005-05-15 00:00:00

  • Viral infection. II. Hemin induces overexpression of p67 as it partially prevents appearance of an active p67-deglycosylase in baculovirus-infected insect cells.

    abstract::The roles of p67-deglycosylase (p67-DG) in the regulation of protein synthesis in baculovirus-infected insect cells were studied. Like vaccinia viral infection, baculovirus infection of insect cells also induced the appearance of a p67-DG. However, p67-DG activity could not be detected because these cells do not conta...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Saha D,Wu S,Bose A,Chatterjee N,Chakraborty A,Chatterjee M,Gupta NK

    更新日期:1997-06-15 00:00:00

  • Metabolic biomarkers for chronic kidney disease.

    abstract::Chronic kidney disease (CKD) is an increasingly recognized burden for patients and health care systems with high (and growing) global incidence and prevalence, significant mortality, and disproportionately high treatment costs. Yet, the available diagnostic tools are either impractical in clinical routine or have seri...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章,评审


    authors: Breit M,Weinberger KM

    更新日期:2016-01-01 00:00:00

  • Identification and characterization of two new types of bacterial L-serine dehydratases and assessment of the function of the ACT domain.

    abstract::Two new types of bacterial Fe-S L-serine dehydratases have been identified. These join two previously recognized enzyme types, for a total of four, that are distinguished on the basis of domain arrangement and amino acid sequence. A Type 3 enzyme from Amphibacillus xylanus (axLSD) and a Type 4 enzyme from Heliscomenob...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Xu XL,Grant GA

    更新日期:2013-12-01 00:00:00

  • Post-translational modifications of myofilament proteins involved in length-dependent prolongation of relaxation in rabbit right ventricular myocardium.

    abstract::The phosphorylation state of several cardiac myofilament proteins changes with the level of stretch in intact, twitch-contracting cardiac muscles. It remains unclear which kinases are involved in the length-dependent phosphorylation of these proteins. We set out to investigate which kinases are involved after a step-w...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Monasky MM,Taglieri DM,Jacobson AK,Haizlip KM,Solaro RJ,Janssen PM

    更新日期:2013-07-01 00:00:00

  • Heme: a regulator of rat hepatic tryptophan 2,3-dioxygenase?

    abstract::The hepatic cytosolic hemoprotein tryptophan 2,3-dioxygenase (TDO) is the rate-limiting enzyme in tryptophan catabolism and thus plays a key role in regulating the physiological flux of tryptophan into relevant metabolic pathways. The TDO protein is induced by corticosteroids such as dexamethasone (DEX) and is stabili...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Ren S,Correia MA

    更新日期:2000-05-01 00:00:00

  • Structural selectivity and molecular nature of L-glutamate transport in cultured human fibroblasts.

    abstract::Uptake of L-[3H]glutamate by monolayers of fibroblasts cultured from human embryonic skin has been studied in the presence of several nonradioactive structural analogs of glutamate and aspartate. Results have suggested that the structural specificites of glutamate transporters in cultured human fibroblasts are similar...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Cooper B,Chebib M,Shen J,King NJ,Darvey IG,Kuchel PW,Rothstein JD,Balcar VJ

    更新日期:1998-05-15 00:00:00

  • Molecular characterization of the human CRT-1 creatine transporter expressed in Xenopus oocytes.

    abstract::The protein sequence encoded by a creatine transporter cDNA cloned from a human heart library was identical to that cloned from a human kidney library (Nash et al., Receptors Channels 2, 165-174, 1994), except that at position 285 the former contained an Ala residue and the latter contained a Pro residue. Expression o...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Dai W,Vinnakota S,Qian X,Kunze DL,Sarkar HK

    更新日期:1999-01-01 00:00:00

  • Enzymatic conversion of the antibiotic metronidazole to an analog of thiamine.

    abstract::We propose that adverse effects of the antibiotic metronidazole may be due, wholly or in part, to its conversion to a thiamine analog and consequent vitamin B1 antagonism. Consistent with this hypothesis, the drug is accepted as a substrate for the thiaminase (EC elaborated as an exoenzyme by the human gut fl...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Alston TA,Abeles RH

    更新日期:1987-09-01 00:00:00

  • Identification of the FTBL protein of Sumner and Dounce as a leucine aminopeptidase.

    abstract::The crystalline beef liver protein of Sumner and Dounce (A. L. Dounce, P. Z. Allen, and G. A. Mourtzikos (1978) Arch. Biochem. Biophys. 188, 251-265) termed FTBL (football) protein because of the shape of its crystals, has been identified as a crystalline leucine aminopeptidase (LAP), on the basis of its high specific...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Dounce AL,Allen PZ

    更新日期:1987-08-15 00:00:00

  • The effect of essential fatty acid deficiency on the adrenergic activation of glycogenolysis in rat hepatocytes.

    abstract::The fatty acid composition of total lipids and the adrenoceptor-mediated activation of glycogenolysis were studied in isolated hepatocytes from rats maintained on a control diet or on an essential fatty acid (EFA)-free diet. In cells from rats on the EFA-free diet there was a marked reduction in linoleic and arachidon...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Grojec MS,Ishac EJ,Kapocsi J,Kunos G

    更新日期:1990-11-15 00:00:00

  • Characterization of the peribacteroid membrane ATPase of lupin root nodules.

    abstract::Peribacteroid membranes can be isolated in essentially pure form from 20-day lupin root nodules by osmotic shock of the purified membrane enclosed bacteroids. The ATPase (EC associated with this membrane has an acid pH optimum (5.25) and is specific for ATP (Mg-ATP Km = 0.16 mM). The enzyme activity requires ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Domigan NM,Farnden KJ,Robertson JG,Monk BC

    更新日期:1988-08-01 00:00:00

  • Regulation of NADP-malate dehydrogenase in C4 plants: relationship among enzyme activity, NADPH to NADP ratios, and thioredoxin redox states in intact maize mesophyll chloroplasts.

    abstract::NADP-malate dehydrogenase activity, the ratio of NADPH to NADP, and thioredoxin redox state in Zea mays chloroplasts were determined after various treatments. Following transfer from dark to light, NADP-malate dehydrogenase was activated more than 20-fold within 10 min while the proportion of pyridine nucleotide as NA...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Rebeille F,Hatch MD

    更新日期:1986-08-15 00:00:00

  • Functionally conservative substitutions at cardiac troponin I S43/45.

    abstract::A phospho-null Ala substitution at protein kinase C (PKC)-targeted cardiac troponin I (cTnI) S43/45 reduces myocyte and cardiac contractile function. The goal of the current study was to test whether cTnIS43/45N is an alternative, functionally conservative substitution in cardiac myocytes. Partial and more extensive e...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Lang SE,Stevenson TK,Xu D,O'Connell R,Westfall MV

    更新日期:2016-07-01 00:00:00

  • Relative activities of retrovirally expressed murine prostaglandin synthase-1 and -2 depend on source of arachidonic acid.

    abstract::We have developed derivatives of mouse embryonic fibroblasts (10T1/2) and Chinese hamster ovary (AS52) cells that stably express high levels of murine prostaglandin synthase-1 or -2 (PGHS-1 or -2). The cDNAs were transferred using retroviral vectors and the resulting G418-resistant clones were analyzed for prostagland...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Chulada PC,Loftin CD,Winn VD,Young DA,Tiano HF,Eling TE,Langenbach R

    更新日期:1996-06-15 00:00:00

  • Regulation of aldose reductase activity by tubulin and phenolic acid derivates.

    abstract::In this work we demonstrate that aldose reductase (AR) interacts directly with tubulin and, was subjected to microtubule formation conditions, enzymatic AR activity increased more than sixfold. Since AR interacts mainly with tubulin that has 3-nitro-tyrosine in its carboxy-terminal, we evaluated whether tyrosine and o...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Rivelli JF,Ochoa AL,Santander VS,Nigra A,Previtali G,Casale CH

    更新日期:2018-09-15 00:00:00

  • Effect of brief treatment at alkaline pH on the properties of UDP-glucuronosyltransferase.

    abstract::The kinetic properties of UDP-glucuronosyltransferase were measured after brief treatment of liver microsomes at alkaline pH, followed by assay with p-nitro-phenol as aglycone, at pH 7.5. Enzyme activity increased in a graded fashion as the pH of pretreatment was increased above 8.0, with apparent maximal activation o...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Dannenberg A,Wong T,Zakim D

    更新日期:1990-03-01 00:00:00

  • Mangiferin, a natural occurring glucosyl xanthone, increases susceptibility of rat liver mitochondria to calcium-induced permeability transition.

    abstract::Mitochondrial permeability transition (MPT) is a Ca(2+)-dependent, cyclosporine A-sensitive, non-selective inner membrane permeabilization induced by a wide range of agents or conditions, which has often been associated with necrotic or apoptotic cell death. When mitochondria isolated from livers of rats treated with ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Andreu GL,Delgado R,Velho JA,Curti C,Vercesi AE

    更新日期:2005-07-15 00:00:00

  • Up-regulation of hyaluronan synthase genes in cultured human epidermal keratinocytes by UVB irradiation.

    abstract::Hyaluronan controls keratinocyte proliferation and regeneration. We examined effect of UV on the expression of hyaluronan synthases (HASs) and hyaluronidases in cultured normal human newborn foreskin epidermal keratinocytes, NHEK(F). HAS3 mRNA was expressed predominantly and HAS2 mRNA expressed in lesser amounts and b...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Kakizaki I,Itano N,Kimata K,Hanada K,Kon A,Yamaguchi M,Takahashi T,Takagaki K

    更新日期:2008-03-01 00:00:00

  • Suppression of c-Src activity stimulates muscle differentiation via p38 MAPK activation.

    abstract::Role of c-Src in muscle differentiation has been controversial. Here, we investigated if c-Src positively or negatively regulates muscle differentiation, using H9c2 and C2C12 cell lines. Inhibition of c-Src by treatment with PP1 and SU6656, pharmacologic inhibitors of Src family kinases, or by expression of a dominant...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Lim MJ,Seo YH,Choi KJ,Cho CH,Kim BS,Kim YH,Lee J,Lee H,Jung CY,Ha J,Kang I,Kim SS

    更新日期:2007-09-01 00:00:00

  • Role of cytosolic NAD(P)H-quinone oxidoreductase and alcohol dehydrogenase in the reduction of p-nitrosophenol following chronic ethanol ingestion.

    abstract::Rats fed an ethanol-containing diet for 4 weeks showed a 3- to 5-fold increase over isocalorically pair-fed controls with respect to cytosolic NAD(P)H-quinone oxidoreductase (NQOR) (E.C. with both menadione and dichlorophenol-indophenol as substrates. Rates of NAD(P)H-dependent p-nitrosophenol (pNSP) reductio...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Hajos AK,Winston GW

    更新日期:1992-06-01 00:00:00

  • Frontal gel chromatography of interacting systems: theoretical and experimental evaluation of the shapes of elution profiles for systems of the type A + B in equilibrium C.

    abstract::A theoretical and experimental study has been made of the advancing elution profile in frontal gel chromatography of interacting systems for which the elution volume of the complex is smaller than that of the larger reactant. First, Gilbert-Jenkins theory is used to delineate the form of the elution profile from the m...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Cann JR,Winzor DJ

    更新日期:1987-07-01 00:00:00

  • Bacterial expression of functional membrane-bound thromboxane synthase having intact sequence and truncated N-terminal hydrophobic segment.

    abstract::A full-length cDNA for human placental thromboxane synthase and a shortened cDNA lacking the sequence corresponding to the N-terminal 2-29 amino acids were expressed in Escherichia coli using a pCW expression vector. Both intact and truncated recombinant enzyme were found in the membrane fraction and were catalyticall...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Xia Z,Tai HH

    更新日期:1995-08-20 00:00:00

  • Raman spectroscopic evidence for a disulfide bridge in calf gamma II crystallin.

    abstract::Laser Raman spectroscopy has been applied to native and dithiothreitol-treated bovine cortical gamma II crystallin to examine the state of the thiol groups and the presence of a putative disulfide bridge. The data reveal significant differences in two key spectral regions. In the thiol stretching region (2500-2600 cm-...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Pande J,McDermott MJ,Callender RH,Spector A

    更新日期:1989-02-15 00:00:00

  • Modulation of the activity of NAD malic enzyme from solanum tuberosum by changes in oligomeric state.

    abstract::The effects of pH, NaCl, and malate2- on the equilibrium between dimeric and higher-molecular-weight forms of NAD malic enzyme from Solanum tuberosum var. Chieftain have been analyzed by monitoring the kinetic changes associated with disaggregation [S. D. Grover and R. T. Wedding (1982) Plant Physiol. 70, 1169-1172]. ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Grover SD,Wedding RT

    更新日期:1984-11-01 00:00:00