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Amino acid sequence of a new type of toxic phospholipase A2 from the venom of the Australian tiger snake (Notechis scutatus scutatus).

Abstract：

:Venom from the common tiger snake, Notechis scutatus scutatus, contains several toxic acidic proteins which promote hypotension and hemorrhage in mice. One of these toxins, HTe, has a phospholipase A2 (PLA2) amino acid sequence. It contains 125 amino acids rather than the 119/120 found in other N. s. scutatus PLA2s, because it also has the loop of residues (62-66) found in helix D of pancreatic PLA2s, the gamma-subunit of taipoxin, and the D-subunit of textilotoxin. High sequence identity is found between the first 57 and the last 25 amino acids of HTe and other N. s. scutatus PLA2s. In the central section containing the pancreatic loop and the beta-wing, sequence similarity with other N. s. scutatus PLA2s is low. The beta-wing amino acids are highly homologous to taipoxin-gamma. HTg, an isoform of HTe, has a sequence almost identical to that of HTe in the central section. Neuropharmacological and neurophysiological studies show that HTe blocks neuromuscular transmission, but it does not produce blockade by virtue of a selective action on nerve endings. Instead, the toxin acts both on nerve and on muscle. Unlike taipoxin-gamma and textilotoxin-D, HTe and HTg are not glycosylated and are not otherwise modified. HTe, HTg, and the other acidic proteins hydrolyze the synthetic PLA2 substrate, 3-octanoyloxy-4-nitrobenzoic acid, as well as L-alpha-phosphatidylcholine.

journal_name

Arch Biochem Biophys

journal_title

Archives of biochemistry and biophysics

authors

Francis B,Coffield JA,Simpson LL,Kaiser II

doi

10.1006/abbi.1995.1257

subject

Has Abstract

pub_date

1995-04-20 00:00:00

pages

481-8

issue

2

eissn

0003-9861

issn

1096-0384

pii

S0003-9861(85)71257-X

journal_volume

318

pub_type

杂志文章

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