Effects of oxidants on properties of fluorescent calcium indicators.

abstract：:Conversion of geranyl pyrophosphate to cyclic monoterpenes is considered to involve the preliminary isomerization of this acyclic precursor to enzyme-bound linalyl pyrophosphate and the cyclization of this tertiary intermediate. 2-Fluorogeranyl pyrophosphate and 2-fluorolinalyl pyrophosphate are effective competitive ...

journal_title：Archives of biochemistry and biophysics

authors： Croteau R

更新日期：1986-12-01 00:00:00

abstract：:A new procedure for the purification of phospholipase C from Clostridium perfringens has been devised that results in essentially pure enzyme. The procedure consists of ammonium sulfate fractionation, ion-exchange chromatography on QAE-Sephadex, and affinity chromatography on phosphatidylcholine linked to Sepharose. T...

journal_title：Archives of biochemistry and biophysics

authors： Krug EL,Kent C

更新日期：1984-06-01 00:00:00

abstract：:The reaction of phospholipid hydroperoxide glutathione peroxidase (PHGPx) and Ebselen with phospholipid and cholesterylester hydroperoxides associated with HDLox and LDLox was investigated using specific HPLC assays for the hydroperoxides of phosphatidylcholine (PCOOH) and cholesteryllinolate (Ch18:2-OOH) and for chol...

journal_title：Archives of biochemistry and biophysics

authors： Sattler W,Maiorino M,Stocker R

更新日期：1994-03-01 00:00:00

abstract：:The electron transfer reactions from FeII(CN)6(4-) and W(IV)(CN)8(4-) to the cupric ions in human copper, zinc superoxide dismutase were followed by the micro-stopped-flow method. The kinetic rate data clearly indicate that FeII(CN)6(4-) or W(IV)(CN)8(4-) first forms an adduct with the enzyme through the interaction w...

journal_title：Archives of biochemistry and biophysics

authors： Hirose J,Minakami M,Settu K,Tsukahara K,Ueda J,Ozawa T

更新日期：2000-11-15 00:00:00

abstract：:Although in vitro models are often used in beta-carotene research, knowledge about the uptake and metabolism of beta-carotene in cell lines is lacking. We measured by HPLC the intracellular levels of beta-carotene and its metabolites in 9 human intestinal and lung cell lines after exposure to 1 microM beta-carotene du...

journal_title：Archives of biochemistry and biophysics

authors： Franssen-van Hal NL,Bunschoten JE,Venema DP,Hollman PC,Riss G,Keijer J

更新日期：2005-07-01 00:00:00

abstract：:Myeloperoxidase (MPO) is a heme-containing peroxidase expressed mainly in neutrophils and to a lesser degree in monocytes. In the presence of hydrogen peroxide and halides, MPO catalyzes the formation of reactive oxygen intermediates, including hypochlorous acid (HOCl). The MPO/HOCl system plays an important role in m...

journal_title：Archives of biochemistry and biophysics

authors： Aratani Y

更新日期：2018-02-15 00:00:00

abstract：:Previous studies in which dicyclohexylcarbodiimide (DCCD) was used to inactivate F1-ATPase enzymes have suggested that two glutamate residues in the beta-subunit are essential for catalysis. In the Escherichia coli F1-ATPase, these are residues beta-Glu-181 and beta-Glu-192. Oligonucleotide-directed mutagenesis was us...

journal_title：Archives of biochemistry and biophysics

authors： Parsonage D,Wilke-Mounts S,Senior AE

更新日期：1988-02-15 00:00:00

abstract：:Purification of equilibrative nucleoside transporters has been hampered by the functional instability of the detergent-solubilized proteins. A variety of nonionic detergents were compared with octylglucoside (the most commonly used detergent in this regard) for their abilities to solubilize functionally stable nucleos...

journal_title：Archives of biochemistry and biophysics

authors： Hammond JR,Zarenda M

更新日期：1996-08-15 00:00:00

abstract：:Bovine heart muscle microsomes rapidly convert lysophosphatidylcholine (LPC) into phosphatidylcholine (PC) in the presence of oleoyl-CoA. Both substrates are incorporated into the product, although the rate of incorporation of radiolabel into PC from 1-[14C]palmitoyl-LPC was approximately threefold higher than the rat...

journal_title：Archives of biochemistry and biophysics

authors： Sanjanwala M,Sun GY,Cutrera MA,MacQuarrie RA

更新日期：1988-09-01 00:00:00

abstract：:This paper presents previously unobserved signals in the 1H NMR spectra of oxidized and reduced [2Fe-2S]-ferredoxin from Anabaena 7120 detected at 400, 500, and 600 MHz. The signals shifted to low field exhibited longitudinal relaxation (T1) values in the range of 100-400 microseconds and line widths in the range of 1...

journal_title：Archives of biochemistry and biophysics

authors： Skjeldal L,Westler WM,Markley JL

更新日期：1990-05-01 00:00:00

abstract：:The relationship between erythroid delta-aminolevulinate (ALA) synthase and hepatic ALA synthase in rat was analyzed immunochemically, using antibodies directed against rat liver ALA synthase and against chicken liver ALA synthase. Rat erythroid ALA synthase showed no cross-reactivity with anti-liver ALA synthase anti...

journal_title：Archives of biochemistry and biophysics

authors： Yamamoto M,Fujita H,Watanabe N,Hayashi N,Kikuchi G

更新日期：1986-02-15 00:00:00

abstract：:An in vitro expression plasmid (pGRAP) that contained the cDNA coding for the rat mitochondrial aldehyde dehydrogenase precursor was constructed, mRNA was synthesized then translated, and the in vitro synthesized precursor of aldehyde dehydrogenase was used in an in vitro import assay. As expected the 19 amino acid si...

journal_title：Archives of biochemistry and biophysics

authors： Wang TT,Farrés J,Weiner H

更新日期：1989-08-01 00:00:00

abstract：:We have reported that dietary long-chain triacylglycerols (LCT) enhance the transcription of cellular retinol-binding protein, the type II (CRBPII) gene, and the liver-type fatty acid-binding protein (L-FABP) gene in the small intestine. Because the cis elements on the CRBPII gene consisting of two AGGTCA motifs separ...

journal_title：Archives of biochemistry and biophysics

authors： Mochizuki K,Suruga K,Kitagawa M,Takase S,Goda T

更新日期：2001-05-01 00:00:00

abstract：:Providing clean and affordable drinking water without harmful disinfection byproducts generated by conventional chemical disinfectants gives rise to the need for technological innovation. Nanotechnology has great potential in purifying water and wastewater treatment. A graphene oxide-silver (GO-Ag) nanocomposite with ...

journal_title：Archives of biochemistry and biophysics

authors： Song B,Zhang C,Zeng G,Gong J,Chang Y,Jiang Y

更新日期：2016-08-15 00:00:00

abstract：:A ganglioside GM1-specific alpha 1-->2fucosyltransferase is induced during the early stages of chemical carcinogenesis with N-2-acetylaminofluorene (AAF) in rat liver hepatocytes. The induction of this enzyme gives rise to the expression of a fucose-containing ganglioside with the same determinant structure as blood g...

journal_title：Archives of biochemistry and biophysics

authors： Sherwood AL,Holmes EH

更新日期：1998-07-15 00:00:00

abstract：:We explored whether modulation of the estrogen receptor (ER) signaling is possible through an aryl hydrocarbon receptor nuclear translocator (Arnt)-dependent mechanism. We utilized the Arnt-interacting protein 2 (Ainp2) to examine whether the presence of Ainp2 in MCF-7 cells would interfere with the Arnt-mediated ER s...

journal_title：Archives of biochemistry and biophysics

authors： Li Y,Li Y,Zhang T,Chan WK

更新日期：2010-10-15 00:00:00

abstract：:Conversion of arachidonic acid into the vicinal diol fatty acid 12R,13S-dihydroxy-5Z,8Z,10E,14Z-eicosatetraenoic acid using an acetone powder of the marine red alga, Gracilariopsis lemaneiformis, occurred via intermediate formation of 12S-hydroperoxy-5Z,8Z,10E,14Z-eicosatetraenoic acid. Incubations of the linoleic aci...

journal_title：Archives of biochemistry and biophysics

authors： Gerwick WH,Moghaddam M,Hamberg M

更新日期：1991-11-01 00:00:00

abstract：:X-ray crystallographic studies on glucose oxidase showed a strong interaction between carbohydrate and protein moieties of the glycoprotein. However, experimental studies under physiological conditions reported no influence of carbohydrate moiety on the structural and functional properties of glucose oxidase. In order...

journal_title：Archives of biochemistry and biophysics

authors： Akhtar MS,Bhakuni V

更新日期：2003-05-15 00:00:00

abstract：:A novel serum protein inhibiting specifically the enzymatic activity of the basic phospholipase A(2) (PLA(2)) from the venom of the Chinese mamushi snake (Agkistrodon blomhoffii siniticus) was purified from a nonvenomous Colubridae snake, Elaphe quadrivirgata. The purified inhibitor was a 150-kDa glycoprotein having a...

journal_title：Archives of biochemistry and biophysics

authors： Okumura K,Inoue S,Ikeda K,Hayashi K

更新日期：2002-12-01 00:00:00

abstract：:Studies of modification of hemoglobin and of sickle hemoglobin by alternative aspirins have been extended to a series of new bis esters with a variety of substituted bridging diacids and to a group of mono esters with polar acyl groups. Rates of hydrolysis of these alternative aspirins have also been examined, and the...

journal_title：Archives of biochemistry and biophysics

authors： Delaney EJ,Massil SE,Shi GY,Klotz IM

更新日期：1984-02-01 00:00:00

abstract：:Vitamin D was discovered as an anti-rachitic agent, but even at present, there is no direct evidence to support the concept that vitamin D directly stimulates osteoblastic bone formation and mineralization. It appears to be paradoxical, but vitamin D functions in the process of osteoclastic bone resorption. In 1952, C...

journal_title：Archives of biochemistry and biophysics

authors： Suda T,Takahashi F,Takahashi N

更新日期：2012-07-01 00:00:00

abstract：:The stability of the Glossoscolex paulistus hemoglobin (HbGp), in two iron oxidation states (and three forms), as monitored by optical absorption, fluorescence emission and circular dichroism (CD) spectroscopies, in the presence of the chaotropic agent urea, is studied. HbGp oligomeric dissociation, denaturation and i...

journal_title：Archives of biochemistry and biophysics

authors： Carvalho FA,Santiago PS,Tabak M

更新日期：2012-03-01 00:00:00

abstract：:The reversible oxidation of protein cysteine residues is well recognized as an important regulatory mechanism in redox-dependent cell signaling. Cysteine oxidation is diverse in nature and involves various post-translational modifications (sulfenic acids, disulfides, etc.) and the specific functional or structural imp...

journal_title：Archives of biochemistry and biophysics

authors： Heppner DE,Janssen-Heininger YMW,van der Vliet A

更新日期：2017-02-15 00:00:00

abstract：:We have developed a protocol to purify apo-ovine (o) prostaglandin endoperoxide H(2) synthase-1 (PGHS-1) to homogeneity from ram seminal vesicles. The resulting apo enzyme can then be reconstituted with Co(3+)-protoporphyrin IX instead of Fe(3+)-protoporphyrin IX to produce a native-like, but functionally inert, enzym...

journal_title：Archives of biochemistry and biophysics

authors： Malkowski MG,Theisen MJ,Scharmen A,Garavito RM

更新日期：2000-08-01 00:00:00

abstract：:The single cysteine residue of human serum albumin (HSA-SH) is the most abundant plasma thiol. HSA transports fatty acids (FA), a cargo that increases under conditions of diabetes, exercise or adrenergic stimulation. The stearic acid-HSA (5/1) complex reacted sixfold faster than FA-free HSA at pH 7.4 with the disulfid...

journal_title：Archives of biochemistry and biophysics

authors： Torres MJ,Turell L,Botti H,Antmann L,Carballal S,Ferrer-Sueta G,Radi R,Alvarez B

更新日期：2012-05-01 00:00:00

abstract：:The soluble form of mitochondrial adenosine triphosphatase was purified in an electrophoretically and immunologically pure form from sweet potato root tissue. The enzyme consisted of six kinds of subunits with different molecular weights (52,500, 51,500, 35,500, 26,000, 23,000, and 12,000), and its molecular weight wa...

journal_title：Archives of biochemistry and biophysics

authors： Iwasaki Y,Asahi T

更新日期：1983-11-01 00:00:00

abstract：:Phospholipase C-gamma1, a substrate for many growth factor receptor and nonreceptor tyrosine kinases, produces second messenger molecules that are elements of signal transduction pathways related to cell proliferation. The influence of deletion mutations, which do not intrude on the domains required for catalytic func...

journal_title：Archives of biochemistry and biophysics

authors： Horstman DA,Chattopadhyay A,Carpenter G

更新日期：1999-01-01 00:00:00

abstract：:No larger molecular weight component appeared in the high-performance gel chromatogram when calcium alone was added to the riboflavin-binding protein solution (RfBP), indicating that calcium alone did not aggregate it. RfBP bound calcium, but the amount of bound calcium decreased markedly upon dephosphorylation. Cross...

journal_title：Archives of biochemistry and biophysics

authors： Aoki T,Yamao Y,Yonemasu E,Kumasaki Y,Kako Y

更新日期：1993-09-01 00:00:00

abstract：:Peptaibols are linear non ribosomal peptides which have been the object of intense research efforts regarding their synthesis and the elucidation of the mechanism allowing their insertion in biological membranes. Forty years after their discovery they are still considered as model compounds and suitable probes for the...

journal_title：Archives of biochemistry and biophysics

authors： Das S,Ben Haj Salah K,Djibo M,Inguimbert N

更新日期：2018-11-15 00:00:00

abstract：:Bone-forming cells originate from distinct embryological layers, mesoderm (axial and appendicular bones) and ectoderm (precursor of neural crest cells, which mainly form facial bones). These cells will develop bones by two principal mechanisms: intramembranous and endochondral ossification. In both cases, condensation...

journal_title：Archives of biochemistry and biophysics

authors： Paiva KB,Granjeiro JM

更新日期：2014-11-01 00:00:00