Abstract:
:Providing clean and affordable drinking water without harmful disinfection byproducts generated by conventional chemical disinfectants gives rise to the need for technological innovation. Nanotechnology has great potential in purifying water and wastewater treatment. A graphene oxide-silver (GO-Ag) nanocomposite with excellent antibacterial activity was prepared and characterized by transmission electron microscope and X-ray photoelectron spectroscopy. The tests were carried out using Escherichia coli and Staphylococcus aureus as model strains of Gram-negative and Gram-positive bacteria, respectively. The effect of bactericide dosage and pH on antibacterial activity of GO-Ag was examined. Morphological observation of bacterial cells by scanning electron microscope showed that GO-Ag was much more destructive to cell membrane of Escherichia coli than that of Staphylococcus aureus. Experiments were carried out using catalase, superoxide dismutase and sodium thioglycollate to investigate the formation of reactive oxygen species and free silver ions in the bactericidal process. The activity of intracellular antioxidant enzymes was measured to investigate the potential role of oxidative stress. According to the consequence, synergetic mechanism including destruction of cell membranes and oxidative stress accounted for the antibacterial activity of GO-Ag nanocomposites. All the results suggested that GO-Ag nanocomposites displayed a good potential for application in water disinfection.
journal_name
Arch Biochem Biophysjournal_title
Archives of biochemistry and biophysicsauthors
Song B,Zhang C,Zeng G,Gong J,Chang Y,Jiang Ydoi
10.1016/j.abb.2016.04.018subject
Has Abstractpub_date
2016-08-15 00:00:00pages
167-76eissn
0003-9861issn
1096-0384pii
S0003-9861(16)30152-7journal_volume
604pub_type
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pub_type: 杂志文章,评审
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更新日期:2013-03-01 00:00:00