Glycogen synthase turnover and phosphorylation in rat H4IIE hepatoma cells.

Abstract:

:Glycogen synthase was isolated from rat H4IIE hepatoma cells by the use of specific antibodies. Immunoprecipitates from cells grown in the presence of [35S]methionine contained two 35S-labeled polypeptides, designated GS1 and GS2, separable by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Labeling of both species was half-maximal after 3 h and remained constant up to 48 h. When cells were incubated with [32P]-phosphate, 32P was incorporated into both species with similar kinetics, half-maximal labeling occurring after 2-3 h. The steady-state ratio 32P/35S was significantly higher for the lower mobility GS2 polypeptide. Pulse-chase experiments showed that the two subunits followed similar kinetics with respect to 35S-labeling. However, the turnover of 32P on the GS2 subunit was significantly faster (t1/2 approximately 30 min) than that on the GS1 subunit (t1/2 approximately 2 h). We suggest that the two polypeptides represent different phosphorylation states of the glycogen synthase subunit and are rapidly interconverted.

journal_name

Arch Biochem Biophys

authors

Armstrong DJ,Roach PJ

doi

10.1016/0003-9861(89)90344-5

subject

Has Abstract

pub_date

1989-11-15 00:00:00

pages

16-22

issue

1

eissn

0003-9861

issn

1096-0384

pii

0003-9861(89)90344-5

journal_volume

275

pub_type

杂志文章
  • Catalytic oxidation of acetaminophen by tyrosinase in the presence of L-proline: a kinetic study.

    abstract::A kinetic study of acetaminophen oxidation by tyrosinase in the presence of a physiological nucleophilic agent such as the amino acid L-proline is performed in the present paper. The o-quinone product of the catalytic activity, 4-acetamido-o-benzoquinone, becomes unstable through the chemical addition of L-proline, in...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/s0003-9861(03)00288-1

    authors: Valero E,Varón R,García-Carmona F

    更新日期:2003-08-15 00:00:00

  • Cofactor and tryptophan accessibility and unfolding of brain glutamate decarboxylase.

    abstract::Cofactor and tryptophan accessibility of the 65-kDa form of rat brain glutamate decarboxylase (GAD) was investigated by fluorescence quenching measurements using acrylamide, I-, and Cs+ as the quenchers. Trp residues were partially exposed to solvent. I- was less able and Cs+ was more able to quench the fluorescence o...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1006/abbi.2001.2466

    authors: Rust E,Martin DL,Chen CH

    更新日期:2001-08-15 00:00:00

  • The role of cytochrome P450 2B6 and 2B4 substrate access channel residues predicted based on crystal structures of the amlodipine complexes.

    abstract::Recent X-ray crystal structures of human cytochrome P450 2B6 and rabbit cytochrome P450 2B4 in complex with amlodipine showed two bound ligand molecules, one in the active site and one in the substrate access channel. Based on the X-ray crystal structures, we investigated the interactions of P450 2B4 and 2B6 with amlo...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/j.abb.2014.01.008

    authors: Jang HH,Davydov DR,Lee GY,Yun CH,Halpert JR

    更新日期:2014-03-01 00:00:00

  • The 1 alpha-hydroxylation of 24-hydroxyvitamin D3 by chick kidney homogenates.

    abstract::Tritium-labeled 24(R)-hydroxyvitamin D3 and 24(S)-hydroxyvitamin D3 were chemically synthesized and the 1 alpha-hydroxylation of these compounds by chick kidney homogenates was studied. A marked stereospecific preference with regard to the orientation of the hydroxyl functionality on carbon-24 was noted: while the 24(...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/0003-9861(83)90115-7

    authors: Ishizuka S,Bannai K,Norman AW

    更新日期:1983-09-01 00:00:00

  • Force-flow and back-pressure relationships in mitochondrial energy transduction: an examination of extended state 3-state 4 transitions.

    abstract::Liver mitochondria were incubated through extended State 3-State 4 transitions (B. Chance and G. N. Williams (1955) J. Biol. Chem. 217, 409-423) in the presence of high concentrations of adenine nucleotides and in presence and absence of a protonophore. In the terminal phase of these transitions (the region of respira...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/0003-9861(89)90391-3

    authors: Davis EJ,Davis-van Thienen WI

    更新日期:1989-12-01 00:00:00

  • Modulation of the reactivity of the thiol of human serum albumin and its sulfenic derivative by fatty acids.

    abstract::The single cysteine residue of human serum albumin (HSA-SH) is the most abundant plasma thiol. HSA transports fatty acids (FA), a cargo that increases under conditions of diabetes, exercise or adrenergic stimulation. The stearic acid-HSA (5/1) complex reacted sixfold faster than FA-free HSA at pH 7.4 with the disulfid...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/j.abb.2012.03.011

    authors: Torres MJ,Turell L,Botti H,Antmann L,Carballal S,Ferrer-Sueta G,Radi R,Alvarez B

    更新日期:2012-05-01 00:00:00

  • Fatostatin inhibits the development of endometrial carcinoma in endometrial carcinoma cells and a xenograft model by targeting lipid metabolism.

    abstract::Endometrial carcinoma is a type of gynecological cancer that originates in the endometrial epithelial tissue. Due to its high proliferation and ability to invade muscle tissue, it is one of the most common malignant tumors in the female reproductive system. Fatostatin is a small molecule non-sterol diarylthiazole deri...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/j.abb.2020.108327

    authors: Yao L,Chen S,Li W

    更新日期:2020-05-15 00:00:00

  • Association of a heat-stable inhibitor protein with cyclic-3',5'-AMP-dependent protein kinase from the nematode Ascaris suum: purification and characterization of the inhibitor.

    abstract::An inhibitor protein of the catalytic subunit of the cyclic 3',5'-AMP-dependent protein kinase from the nematode Ascaris suum was isolated and characterized. The molecular weight of the inhibitor was estimated as 28,000 by electrophoresis under denaturing conditions and as 30,000 by gel permeation chromatography on Su...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/0003-9861(92)90676-n

    authors: Hoffmann R,Jung S,Hofer HW

    更新日期:1992-09-01 00:00:00

  • Induction of refractoriness of cyclic AMP responses to prostaglandin E1 and epinephrine by prior exposure of guinea pig macrophages to lipopolysaccharide.

    abstract::Prior exposure of guinea pig macrophages to LPS (lipopolysaccharide) resulted in reduced cAMP-generating responses to prostaglandin E1 and epinephrine. LPS-induced refractoriness was diminished when LPS treatment was carried out in the presence of an inhibitor of prostaglandin synthesis, hydrocortisone, or indomethaci...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/0003-9861(86)90334-6

    authors: Hazeki K,Mori Y,Ui M

    更新日期:1986-05-01 00:00:00

  • Disruption of mitochondrial functions and oxidative stress contribute to neurologic dysfunction in organic acidurias.

    abstract::Organic acidurias (OADs) are inherited disorders of amino acid metabolism biochemically characterized by accumulation of short-chain carboxylic acids in tissues and biological fluids of the affected patients and clinically by predominant neurological manifestations. Some of these disorders are amenable to treatment, w...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章,评审

    doi:10.1016/j.abb.2020.108646

    authors: Wajner M,Vargas CR,Amaral AU

    更新日期:2020-12-15 00:00:00

  • Effect of brief treatment at alkaline pH on the properties of UDP-glucuronosyltransferase.

    abstract::The kinetic properties of UDP-glucuronosyltransferase were measured after brief treatment of liver microsomes at alkaline pH, followed by assay with p-nitro-phenol as aglycone, at pH 7.5. Enzyme activity increased in a graded fashion as the pH of pretreatment was increased above 8.0, with apparent maximal activation o...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/0003-9861(90)90585-m

    authors: Dannenberg A,Wong T,Zakim D

    更新日期:1990-03-01 00:00:00

  • Partial biochemical and immunochemical characterization of avian eggshell extracellular matrices.

    abstract::There is evidence to suggest that extracellular matrix molecules, such as proteoglycans, are involved in the regulation of mineral deposition in calcifying tissues. One mineralizing system which is characterized by extremely rapid mineralization is the hen eggshell. This eggshell consists of a pair of nonmineralized e...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/0003-9861(92)90126-h

    authors: Arias JL,Carrino DA,Fernández MS,Rodríguez JP,Dennis JE,Caplan AI

    更新日期:1992-10-01 00:00:00

  • Myeloperoxidase: Its role for host defense, inflammation, and neutrophil function.

    abstract::Myeloperoxidase (MPO) is a heme-containing peroxidase expressed mainly in neutrophils and to a lesser degree in monocytes. In the presence of hydrogen peroxide and halides, MPO catalyzes the formation of reactive oxygen intermediates, including hypochlorous acid (HOCl). The MPO/HOCl system plays an important role in m...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章,评审

    doi:10.1016/j.abb.2018.01.004

    authors: Aratani Y

    更新日期:2018-02-15 00:00:00

  • CK2-mediated phosphorylation of a type II regulatory subunit of cAMP-dependent protein kinase from the mollusk Mytilus galloprovincialis.

    abstract::Two isoforms of regulatory (R) subunit of cAMP-dependent protein kinase (PKA), named R(myt1) and R(myt2), were identified so far in the sea mussel Mytilus galloprovincialis. Out of them, only R(myt2) was phosphorylated in vitro by casein kinase 2 (CK2) using GTP as phosphate donor. CK2 catalytic subunit (CK2alpha) its...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/j.abb.2007.02.008

    authors: Bardales JR,Hellman U,Villamarín JA

    更新日期:2007-05-01 00:00:00

  • Age-related changes in the composition and mechanical properties of human nasal cartilage.

    abstract::Nasal cartilage is widely used in reconstructive surgery for the replacement of soft tissue defects and nasal reconstruction procedures. The ability to shape harvested tissue and the performance in the transplant site are related to the mechanical properties of nasal cartilage. Several studies have documented changes ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/S0003-9861(02)00263-1

    authors: Rotter N,Tobias G,Lebl M,Roy AK,Hansen MC,Vacanti CA,Bonassar LJ

    更新日期:2002-07-01 00:00:00

  • Induction of the male-specific cytochrome P450 3A2 in female rats by phenytoin.

    abstract::We previously reported that administration of dexamethasone (DEX) and other selected pharmacological agents to rats resulted in a profound increase in hepatic cytochrome P450 3A1 in both sexes, but male constitutive P450 3A2 was modestly increased (4-fold) in adult males and not detected in either treated or untreated...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1006/abbi.1996.0327

    authors: Ghosal A,Sadrieh N,Reik L,Levin W,Thomas PE

    更新日期:1996-08-01 00:00:00

  • Translocation of deltaPKC to mitochondria during cardiac reperfusion enhances superoxide anion production and induces loss in mitochondrial function.

    abstract::Activation of the delta-isoform of protein kinase C (deltaPKC) by certain conditions of oxidative stress results in translocation of the kinase to the mitochondria leading to release of cytochrome c and the induction of apoptosis. In the current study, the effects of myocardial reperfusion-induced deltaPKC translocati...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/j.abb.2005.05.007

    authors: Churchill EN,Szweda LI

    更新日期:2005-07-15 00:00:00

  • Structural and kinetic characterization of two 4-oxalocrotonate tautomerases in Methylibium petroleiphilum strain PM1.

    abstract::Methylibium petroleiphilum strain PM1 uses various petroleum products including the fuel additive methyl tert-butyl ether and straight chain and aromatic hydrocarbons as sole carbon and energy sources. It has two operons, dmpI and dmpII, that code for the enzymes in a pair of parallel meta-fission pathways. In order t...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/j.abb.2013.06.016

    authors: Terrell CR,Burks EA,Whitman CP,Hoffman DW

    更新日期:2013-09-01 00:00:00

  • Peroxidase-catalyzed formation of quercetin quinone methide-glutathione adducts.

    abstract::The oxidation of quercetin by horseradish peroxidase/H(2)O(2) was studied in the absence but especially also in the presence of glutathione (GSH). HPLC analysis of the reaction products formed in the absence of GSH revealed formation of at least 20 different products, a result in line with other studies reporting the ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1006/abbi.2000.1832

    authors: Awad HM,Boersma MG,Vervoort J,Rietjens IM

    更新日期:2000-06-15 00:00:00

  • Polyphenols and health: update and perspectives.

    abstract::Current issues in research on health effects by polyphenols are addressed. As to the cardiovascular system, flow-mediated dilation (FMD), a functional biomarker, can be used as surrogate marker for cardiovascular risk. Acute short-term effects peaking at 2h after ingestion of polyphenol-rich food items are distinguish...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章,评审

    doi:10.1016/j.abb.2010.04.006

    authors: Sies H

    更新日期:2010-09-01 00:00:00

  • Docking of verapamil in a synthetic Ca2+ channel: formation of a ternary complex involving Ca2+ ions.

    abstract::The mechanism by which diverse drugs modulate voltage-dependent Ca2+ channels is ill-understood. We have approached this problem by examining the interaction of verapamil with a 97-residue synthetic channel peptide (SCP) that exhibits functional similarities to authentic L-type Ca2+ channels in terms of cation selecti...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1006/abbi.1997.9968

    authors: Zhorov BS,Ananthanarayanan VS

    更新日期:1997-05-15 00:00:00

  • Gene expression of iron-related proteins during iron deficiency caused by scurvy in guinea pigs.

    abstract::The regulation of expression of hepatic iron-related proteins was examined during iron deficiency caused by scurvy in guinea pigs. Previous studies showed that some effects of scurvy, such as suppression of collagen gene expression, result from events associated with weight loss. During the initial phase of scurvy whe...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1006/abbi.1996.0037

    authors: Gosiewska A,Mahmoodian F,Peterkofsky B

    更新日期:1996-01-15 00:00:00

  • In vivo and in vitro binding of platinum to metallothionein.

    abstract::The in vivo binding of platinum to metallothionein (MT) has been observed in rat tissues following injections of the cis and trans isomers of DDP (dichlorodiammineplatinum(II]. Platinum in either cis-DDP or trans-DDP does not directly induce MT; platinum-MT is produced by the replacement of previously bound zinc in th...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/0003-9861(84)90150-4

    authors: Zelazowski AJ,Garvey JS,Hoeschele JD

    更新日期:1984-02-15 00:00:00

  • Extracellular ATP activates polyphosphoinositide breakdown and Ca2+ mobilization in Ehrlich ascites tumor cells.

    abstract::The effects of extracellular ATP on phosphoinositide metabolism and intracellular Ca2+ homeostasis were studied in Ehrlich ascites tumor cells. Cytosolic [Ca2+] was measured using either quin 2 or the recently described indicator fura 2. Addition of 0.5-25 microM extracellular ATP to intact cells results in a rapid mo...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/0003-9861(86)90192-x

    authors: Dubyak GR

    更新日期:1986-02-15 00:00:00

  • Structural and functional characterization of the first intracellular loop of human thromboxane A2 receptor.

    abstract::The conformation of a constrained peptide mimicking the putative first intracellular domain (iLP1) of thromboxane A(2) receptor (TP) was determined by (1)H 2D NMR spectroscopy. Through completed assignments of TOCSY, DQF-COSY, and NOESY spectra, a NMR structure of the peptide showed a beta-turn in residues 56-59 and a...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/j.abb.2004.01.001

    authors: Geng L,Wu J,So SP,Huang G,Ruan KH

    更新日期:2004-03-15 00:00:00

  • Oxygenation of polyunsaturated long chain fatty acids by recombinant CYP4F8 and CYP4F12 and catalytic importance of Tyr-125 and Gly-328 of CYP4F8.

    abstract::Recombinant CYP4F8 and CYP4F12 metabolize prostaglandin H2 (PGH2) analogs by omega2- and omega3-hydroxylation and arachidonic acid (20:4n-6) by omega3-hydroxylation. CYP4F8 was found to catalyze epoxidation of docosahexaenoic acid (22:6n-3) and docosapentaenoic acid (22:5n-3) and omega3-hydroxylation of 22:5n-6. CYP4F...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/j.abb.2005.07.003

    authors: Stark K,Wongsud B,Burman R,Oliw EH

    更新日期:2005-09-15 00:00:00

  • Extracellular ATP modulates podocyte function through P2Y purinergic receptors and pleiotropic effects on AMPK and cAMP/PKA signaling pathways.

    abstract::Podocytes and their foot processes interlinked by slit diaphragms, constitute a continuous outermost layer of the glomerular capillary and seem to be crucial for maintaining the integrity of the glomerular filtration barrier. Purinergic signaling is involved in a wide range of physiological processes in the renal syst...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/j.abb.2020.108649

    authors: Szrejder M,Rachubik P,Rogacka D,Audzeyenka I,Rychłowski M,Angielski S,Piwkowska A

    更新日期:2020-11-30 00:00:00

  • The mechanism of helical transition of proteins by organic solvents.

    abstract::This paper describes a theory for the mechanism of three-state transition of proteins which is often observed in aqueous organic cosolvent systems, i.e., from the native, via intermediate to helical forms. The first transition, accompanied by changes in the tertiary and/or secondary structures, was explained by larger...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/0003-9861(85)90004-9

    authors: Arakawa T,Goddette D

    更新日期:1985-07-01 00:00:00

  • Mechanism of the aromatic aminotransferase encoded by the Aro8 gene from Saccharomyces cerevisiae.

    abstract::The amino acid L-lysine is synthesized in Saccharomyces cerevisiae via the α-aminoadipate pathway. An as yet unidentified PLP-containing aminotransferase is thought to catalyze the formation of α-aminoadipate from α-ketoadipate in the L-lysine biosynthetic pathway that could be the yeast Aro8 gene product. A screen of...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1016/j.abb.2011.09.008

    authors: Karsten WE,Reyes ZL,Bobyk KD,Cook PF,Chooback L

    更新日期:2011-12-01 00:00:00

  • Production of a functional anti-scorpion hemocyanin scFv in Escherichia coli.

    abstract::A58 is a murine monoclonal antibody raised against the internal dimeric subunit A alpha 1 of the hemocyanin of the scorpion Androctonus australis. When A58 is tested against a range of related hemocyanins, it exhibits cross-reactivity with a limited number of scorpion species. All of them belong to a single Scorpion F...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章

    doi:10.1006/abbi.1995.1185

    authors: Billiald P,Motta G,Vaux DJ

    更新日期:1995-03-10 00:00:00