Unusual pseudosubstrate specificity of a novel 3,5-dimethoxyphenol O-methyltransferase cloned from Ruta graveolens L.

abstract：:Actinomycin D (act-D) anchors itself into DNA-base pairs by intercalation and thereby inhibits mRNA synthesis. It has been well established that act-D elicits apoptosis in various cell types involving endothelial cells. However, the regulatory mechanisms of actinomycin D-induced apoptotic cell death still remain uncle...

journal_title：Archives of biochemistry and biophysics

authors： Shim D,Kang HY,Jeon BW,Kang SS,Chang SI,Kim HY

更新日期：2004-05-15 00:00:00

abstract：:Modification/chlorination of high-density lipoprotein (HDL) by hypochlorous acid (HOCl), formed by the myeloperoxidase-H₂O₂-chloride system of activated phagocytes, converts an anti-atherogenic lipoprotein into a pro-inflammatory lipoprotein particle. Chlorinated HDL is present in human lesion material, binds to and i...

journal_title：Archives of biochemistry and biophysics

authors： Rossmann C,Rauh A,Hammer A,Windischhofer W,Zirkl S,Sattler W,Malle E

更新日期：2011-05-01 00:00:00

abstract：:By exploiting its capacity for binding to DNA, the protease inhibitor alpha 1-antichymotrypsin has been isolated from human serum by ammonium sulfate fractionation and successive chromatography on QAE-Sephadex, DNA-cellulose, and Sephacryl S-300. This experimental procedure compares favorably with existing methods for...

journal_title：Archives of biochemistry and biophysics

authors： Abdullah M,Siddiqui AA,Hill JA,Davies RJ

更新日期：1983-08-01 00:00:00

abstract：:The pH dependence of the activity of Cu,Zn superoxide dismutases from bovine erythrocytes and shark liver was studied by pulse radiolysis in both the native enzymes and those chemically modified at lysine side chains. The study was aimed at identifying the residues responsible for the activity decrease at pH > 9, obse...

journal_title：Archives of biochemistry and biophysics

authors： Polticelli F,O'Neill P,Costanzo S,Lania A,Rotilio G,Desideri A

更新日期：1995-08-01 00:00:00

abstract：:Elevation of cytosolic level of Ca(2+) was measured by spatial screening of freshly isolated dorsal root ganglion neurons loaded with Fura-2AM after subjecting them to a moderate hypoxic solution (pO(2)=10-40 mmHg). Short exposure of neurons to hypoxia resulted in a reversible elevation of intracellular Ca(2+) to abou...

journal_title：Archives of biochemistry and biophysics

authors： Lukyanetz EA,Stanika RI,Koval LM,Kostyuk PG

更新日期：2003-02-15 00:00:00

abstract：:The macrophage mannose receptor is highly susceptible to modulation by a variety of inflammatory and anti-inflammatory agents. Previous studies have demonstrated that mannose receptor activity is dramatically enhanced in rat bone marrow macrophages following treatment with dexamethasone. In the present study we have i...

journal_title：Archives of biochemistry and biophysics

authors： Cowan HB,Vick S,Conary JT,Shepherd VL

更新日期：1992-07-01 00:00:00

abstract：:Thromboxane A2 synthase (TXAS) binds to the endoplasmic reticulum membrane and catalyzes both an isomerization of prostaglandin H2 (PGH2) to form thromboxane A2 (TXA2) and a fragmentation of PGH2 to form 12-hydroxy-5,8,10-heptadecatrienoic acid (HHT) and malondialdehyde (MDA). TXAS is a non-classic cytochrome P450 in ...

journal_title：Archives of biochemistry and biophysics

authors： Hsu PY,Wang LH

更新日期：2003-08-01 00:00:00

abstract：:Histone-DNA templates for bacteriophage T7 RNA polymerase were assembled from a plasmid containing a promoter and a terminator for this polymerase, (H3 x H4)2 tetramers deprived of their tail domains, and H2A x H2B dimers. Histone (H3 x H4)2 tetramers lacking their terminal domains were obtained from trypsin-digested ...

journal_title：Archives of biochemistry and biophysics

authors： Hernández F,López-Alarcón L,Puerta C,Palacián E

更新日期：1998-10-01 00:00:00

abstract：:The reactive cellular products generated during the peroxidation of membrane lipids have been implicated as causative agents in a variety of degenerative diseases and aging. In particular, 4-hydroxynon-2-enal (4HNE) is among the most of the produced during lipid peroxidation. In humans and rodent species, the alpha 4 ...

journal_title：Archives of biochemistry and biophysics

authors： Gardner JL,Gallagher EP

更新日期：2001-06-01 00:00:00

abstract：:Human seminal fluid was recently found to contain 18,19-dehydroprostaglandins E1 and E2 (E. H. Oliw, H. Sprecher, and M. Hamberg, (1986) J. Biol. Chem. 261, 2675-2683). In the present study, the cis and trans isomers of 18,19-dehydroprostaglandins E1 and E2 were prepared by incubation of microsomes of ram vesicular gl...

journal_title：Archives of biochemistry and biophysics

authors： Oliw EH,Benthin G,Hamberg M

更新日期：1987-10-01 00:00:00

abstract：:A 42-kDa plant outer mitochondrial membrane protein, MOM42, has been identified as an essential component of the plant mitochondrial precursor protein translocation apparatus. Immunological cross-reactivity has been detected between antibodies raised against both Neurospora and yeast mitochondrial outer membrane prote...

journal_title：Archives of biochemistry and biophysics

authors： Perryman RA,Mooney B,Harmey MA

更新日期：1995-02-01 00:00:00

abstract：:The effects of benzyl (BITC) and phenethyl isothiocyanate (PEITC) on the activity of a P450 2E1 mutant where the conserved threonine at position 303 was replaced with an alanine residue (P450 2E1 T303A) were examined. PEITC inactivated the mutant enzyme with a K(I) of 1.6 microM. PEITC also inactivated the wild-type P...

journal_title：Archives of biochemistry and biophysics

authors： Moreno RL,Goosen T,Kent UM,Chung FL,Hollenberg PF

更新日期：2001-07-01 00:00:00

abstract：:High-performance size exclusion chromatography revealed that alkaline-extracted peach fruit pectin dissolved in 0.05 M NaNO3 comprised a hierarchy of at least four aggregated, macromolecular-sized species. Each of the three largest species was found to be comparable in length to the three smallest subunits of an inter...

journal_title：Archives of biochemistry and biophysics

authors： Fishman ML,Cooke P,Levaj B,Gillespie DT,Sondey SM,Scorza R

更新日期：1992-04-01 00:00:00

abstract：:Resonance Raman spectroscopy (RRS) is a non-invasive method that has been developed to assess carotenoid status in human tissues including human skin in vivo. Skin carotenoid status has been suggested as a promising biomarker for human studies. This manuscript describes research done relevant to the development of thi...

journal_title：Archives of biochemistry and biophysics

authors： Mayne ST,Cartmel B,Scarmo S,Jahns L,Ermakov IV,Gellermann W

更新日期：2013-11-15 00:00:00

abstract：:The stabilities of bilirubin (BR) glucuronide, monoglucuronide (BMG), and diglucuronide (BDG) were studied under various conditions by HPLC. In aqueous media, BMG showed a pronounced lability and was easily transformed into equimolar BDG and BR. It was proved by direct analysis of tetrapyrrole isomers that BDG and BR ...

journal_title：Archives of biochemistry and biophysics

authors： Adachi S,Uesugi T,Kamisaka K

更新日期：1985-09-01 00:00:00

abstract：:Glycerol phosphate acyltransferase (GPAT) catalyzes the formation of 1-acyl-sn-glycerol-3-phosphate from glycerol-3-phosphate and long chain fatty acyl-CoA substrates. We previously determined the topography of the mitochondrial GPAT1 isoform (mtGPAT1, 828 amino acids). mtGPAT1 has two transmembrane domains (TMDs) (aa...

journal_title：Archives of biochemistry and biophysics

authors： Pellon-Maison M,Coleman RA,Gonzalez-Baró MR

更新日期：2006-06-15 00:00:00

abstract：:Some compounds are known to induce solute-nonselective permeability of the inner mitochondrial membrane (IMM) in Ca2+-loaded mitochondria. Existing data suggest that this process, following the opening of a mitochondrial permeability transition pore, is preceded by different solute-selective permeable states of IMM. A...

journal_title：Archives of biochemistry and biophysics

authors： Balakirev MY,Zimmer G

更新日期：1998-08-01 00:00:00

abstract：:Under physiological conditions the uptake of transferrin-bound iron by reticulocytes involves transferrin binding to membrane receptors followed by endocytosis, release of iron from the transferrin within endosomes, and recycling of apotransferrin to the cell surface. However, as shown in the present work, iron uptake...

journal_title：Archives of biochemistry and biophysics

authors： Hodgson LL,Quail EA,Morgan EH

更新日期：1994-01-01 00:00:00

abstract：:Chloroplast tRNA(Glu) is a bifunctional molecule involved in both the early steps of chlorophyll synthesis and chloroplast protein biosynthesis. Recently the enzymes involved in these processes have been characterized from the green alga Chlamydomonas reinhardtii. In order to investigate whether transcription of the g...

journal_title：Archives of biochemistry and biophysics

authors： Jahn D

更新日期：1992-11-01 00:00:00

abstract：:Cytochrome P450 (P450) 2C1/2 contains redundant endoplasmic reticulum (ER) retention signals and is excluded from the recycling pathway. Other P450s, such as P450 2E1, have been detected in the plasma membrane and Golgi apparatus. To examine whether the mechanisms of ER retention might differ for P450 2C1/2 and P450 2...

journal_title：Archives of biochemistry and biophysics

authors： Szczesna-Skorupa E,Chen CD,Kemper B

更新日期：2000-02-15 00:00:00

abstract：:If liver alcohol dehydrogenase were rate-limiting in ethanol metabolism, inhibitors of the enzyme should inhibit the metabolism with the same type of kinetics and the same kinetic constants in vitro and in vivo. Against varied concentrations of ethanol, 4-methylpyrazole is a competitive inhibitor of purified rat liver...

journal_title：Archives of biochemistry and biophysics

authors： Plapp BV,Leidal KG,Smith RK,Murch BP

更新日期：1984-04-01 00:00:00

abstract：:A search of the chicken expressed sequence tag (EST) database identified 2 cDNA clones that appeared to represent members of the SULT1B and SULT1C enzyme families. These cDNAs were fully sequenced and found to contain full-length inserts. Phylogenetic analysis of the derived amino acid sequences clearly placed them as...

journal_title：Archives of biochemistry and biophysics

authors： Wilson LA,Reyns GE,Darras VM,Coughtrie MW

更新日期：2004-08-01 00:00:00

abstract：:From mistletoe Viscum album L. extracts three chitin-binding lectin isoforms, cbML1, cbML2, and cbML3, were isolated and their primary structure determined. All three cbML isoforms are composed of two protein chains of 48 or 49 amino acid residues, linked by an intermolecular disulfide bond. The sequence of each singl...

journal_title：Archives of biochemistry and biophysics

authors： Stoeva S,Franz M,Wacker R,Krauspenhaar R,Guthöhrlein E,Mikhailov A,Betzel C,Voelter W

更新日期：2001-08-01 00:00:00

abstract：:Rodobacter capsulatus cells, which were cultured anaerobically in high light intensity, had fewer foldings in the cytoplasmic membrane than those which were grown in lower light intensities. Spheroplast-derived membrane fractions obtained from cells cultured under high light intensity contained a high yield of large r...

journal_title：Archives of biochemistry and biophysics

authors： Carmeli C,Lifshitz Y,Friedberg I

更新日期：1991-08-01 00:00:00

abstract：:60S ribosomal protein L35 (RPL35) is an important component of the 60S ribosomal subunit and has a role in protein translation and endoplasmic reticulum (ER) docking. However, few studies have investigated RPL35 in eukaryotes and much remains to be learned. Here, we analyzed the function of RPL35 in β-casein (CSN2) sy...

journal_title：Archives of biochemistry and biophysics

authors： Jiang N,Hu L,Liu C,Gao X,Zheng S

更新日期：2015-10-01 00:00:00

abstract：BACKGROUND:This study was designed to identify novel links between lipid species and disease progression in non-alcoholic fatty liver disease (NAFLD). METHODS:We analyzed lipid species in the liver and plasma of db/db mice fed a choline-deficient l-amino acid-defined, high-fat diet (CDAHFD) using liquid chromatography...

journal_title：Archives of biochemistry and biophysics

authors： Horas H Nababan S,Nishiumi S,Kawano Y,Kobayashi T,Yoshida M,Azuma T

更新日期：2017-06-01 00:00:00

abstract：:The cellulose synthase protein (AcsAB) is encoded by a single gene in Gluconacetobacter hansenii ATCC 23769. We have examined the processing pattern of this enzyme and the localization of the cleavage products by heterologously expressing the truncated portions of the AcsAB protein and using specific antibodies genera...

journal_title：Archives of biochemistry and biophysics

authors： Iyer PR,Liu YA,Deng Y,McManus JB,Kao TH,Tien M

更新日期：2013-01-15 00:00:00

abstract：:Nitroxides stable radicals are unreactive toward most diamagnetic molecules, but readily undergo one-electron redox reactions with paramagnetic species such as free radicals and transition metals, thus serving as cell-permeable antioxidants. The cytotoxicity of juglone (5-hydroxy-1,4-naphthoquinone), like that of othe...

journal_title：Archives of biochemistry and biophysics

authors： Zhang R,Hirsch O,Mohsen M,Samuni A

更新日期：1994-08-01 00:00:00

abstract：:Sulfhydryl-specific reagents were used to study the reactivities and function of the four cysteinyl residues per subunit present in Salmonella typhimurium 5-phosphoribosyl-alpha-1-pyrophosphate (PRPP) synthetase. In the presence of high concentrations of denaturants all four cysteinyl residues reacted with sulfhydryl-...

journal_title：Archives of biochemistry and biophysics

authors： Harlow KW,Switzer RL

更新日期：1990-02-01 00:00:00

abstract：:Protein synthesis and lipid peroxidation were evaluated in rat liver slices incubated in the presence of oxidants and protein synthesis inhibitors. Protein synthesis by rat liver slices was evaluated by [3H]leucine incorporation into the trichloroacetic acid (TCA)-insoluble material, and lipid peroxidation was evaluat...

journal_title：Archives of biochemistry and biophysics

authors： Fraga CG,Zamora R,Tappel AL

更新日期：1989-04-01 00:00:00