Targeting thioredoxin reductase by plumbagin contributes to inducing apoptosis of HL-60 cells.

abstract：:Protein synthesis and lipid peroxidation were evaluated in rat liver slices incubated in the presence of oxidants and protein synthesis inhibitors. Protein synthesis by rat liver slices was evaluated by [3H]leucine incorporation into the trichloroacetic acid (TCA)-insoluble material, and lipid peroxidation was evaluat...

journal_title：Archives of biochemistry and biophysics

authors： Fraga CG,Zamora R,Tappel AL

更新日期：1989-04-01 00:00:00

abstract：:Previous genetic studies have indicated that the type L alpha-glucan phosphorylase (Pho1) has an essential role during the initiation process of starch biosynthesis during rice seed development. To gain insight into its role in starch metabolism, we characterized the enzymatic properties of the Pho1 recombinant form. ...

journal_title：Archives of biochemistry and biophysics

authors： Hwang SK,Nishi A,Satoh H,Okita TW

更新日期：2010-03-01 00:00:00

abstract：:We demonstrate that the efficiency of adenovirus-assisted exogenous Ca(2+) ATPase (SERCA) and reporter (EGFP) gene expression is much higher in primary cultures of myocytes from neonatal rat hearts, than in primary cultures of myocytes from adult rat hearts. In this respect, the neonatal myocytes behave similarly to t...

journal_title：Archives of biochemistry and biophysics

authors： Sumbilla C,Ma H,Seth M,Inesi G

更新日期：2003-07-15 00:00:00

abstract：:Two isoforms of regulatory (R) subunit of cAMP-dependent protein kinase (PKA), named R(myt1) and R(myt2), were identified so far in the sea mussel Mytilus galloprovincialis. Out of them, only R(myt2) was phosphorylated in vitro by casein kinase 2 (CK2) using GTP as phosphate donor. CK2 catalytic subunit (CK2alpha) its...

journal_title：Archives of biochemistry and biophysics

authors： Bardales JR,Hellman U,Villamarín JA

更新日期：2007-05-01 00:00:00

abstract：:We previously found that vitamin K3 (menadione, 2-methyl-1,4-naphthoquinone) inhibits the activity of human mitochondrial DNA polymerase (pol) γ. In this study, we focused on plumbagin (5-hydroxy-2-methyl-1,4-naphthoquinone), and chemically synthesized novel plumbagins conjugated with C2:0 to C22:6 fatty acids (5-O-ac...

journal_title：Archives of biochemistry and biophysics

authors： Onodera T,Kuriyama I,Sakamoto Y,Kawamura M,Kuramochi K,Tsubaki K,Tabata A,Naganune H,Mizushina Y

更新日期：2015-05-01 00:00:00

abstract：:Short, strong (low barrier) hydrogen bonds occur when the pK values of the atoms sharing the proton are similar. The overall distance is 2.5 A or less, the deuterium fractionation factor is less than 0.5, the proton NMR chemical shift can approach 20 ppm, and deuterium or tritium substitution causes an up-field change...

journal_title：Archives of biochemistry and biophysics

authors： Cleland WW

更新日期：2000-10-01 00:00:00

abstract：:The sites of expression in the small intestine and the function of CYP2J4, a recently identified rat cytochrome (P450) isoform found to be predominantly expressed in the small intestine, were characterized. Immunoblot analysis with a polyclonal antibody to heterologously expressed CYP2J4 revealed that expression of CY...

journal_title：Archives of biochemistry and biophysics

authors： Zhang QY,Raner G,Ding X,Dunbar D,Coon MJ,Kaminsky LS

更新日期：1998-05-15 00:00:00

abstract：:Erythropoietin was purified to homogeneity from the culture medium of a baby hamster kidney cell line stably transfected with a human erythropoietin gene. A three-step procedure was used, which included affinity chromatography, ion-exchange chromatography, and reverse-phase chromatography. Purity of the protein was co...

journal_title：Archives of biochemistry and biophysics

authors： Broudy VC,Tait JF,Powell JS

更新日期：1988-09-01 00:00:00

abstract：:Rabbit skeletal muscle sarcoplasmic reticulum (SR) was fractionated by isopycnic density gradient centrifugation into longitudinal tubules (LSR) and terminal cisternae (TC). Junctional face membranes (JFM) were obtained by Triton X-100 treatment of the TC fraction (Costello, B., Chadwick, C., Saito, A., Chu, A., Maure...

journal_title：Archives of biochemistry and biophysics

authors： Volpe P,Gutweniger HE,Montecucco C

更新日期：1987-02-15 00:00:00

abstract：:Much effort has focused on the identification of MAPK cascades that are activated by the MEKK family of protein kinases. However, direct phosphorylation and regulation of the MEKK proteins has not been shown. To address this question, we have expressed recombinant (His)6FLAG.MEKK3 in Sf9 insect cells and tethered the ...

journal_title：Archives of biochemistry and biophysics

authors： Adams DG,Sachs NA,Vaillancourt RR

更新日期：2002-11-01 00:00:00

abstract：:Triacylglycerol plays a critical role in an organism's ability to withstand fuel deprivation, and dysregulation of triacylglycerol synthesis is important in the development of diseases such as obesity and diabetes. Glycerol-3-phosphate acyltransferase (GPAT) catalyzes the initial and committed step of glycerolipid syn...

journal_title：Archives of biochemistry and biophysics

authors： Lewin TM,Granger DA,Kim JH,Coleman RA

更新日期：2001-12-01 00:00:00

abstract：:Juvenile hormone binding protein (JHBP) acts as a shuttle, carrying one of the most crucial hormones for insect development to target tissues. We have found that although the JHBP molecule does not contain tryptophan residues, it exhibits a weak fluorescence maximum near 420nm upon excitation at 315nm. Gel filtration ...

journal_title：Archives of biochemistry and biophysics

authors： Bystranowska D,Szewczuk Z,Lisowski M,Sitkiewicz E,Dobryszycki P,Ożyhar A,Kochman M

更新日期：2012-01-01 00:00:00

abstract：:Phenylalanine ammonia-lyase (PAL) catalyzes the beta-elimination of ammonia from L-phenylalanine to trans-cinnamic acid. A study of inhibition of PAL by phenol, ortho-cresol, and meta-cresol gave mixed inhibition; para-cresol is not an inhibitor. The calculated values of K(i) and alphaK(i) are phenol, K(i)=2.1+/-0.5 m...

journal_title：Archives of biochemistry and biophysics

authors： Alunni S,Cipiciani A,Fioroni G,Ottavi L

更新日期：2003-04-15 00:00:00

abstract：:Several experiments were carried out to study the difference between two isozymes (alpha(+) and alpha) of (Na+ + K+)-ATPase in the conformational equilibrium. Rat brain (Na+ + K+)-ATPase was much more thermolabile than the kidney enzyme. Both enzymes were protected from heat inactivation not only by Na+ and K+, but al...

journal_title：Archives of biochemistry and biophysics

authors： Matsuda T,Iwata H

更新日期：1988-06-01 00:00:00

abstract：:The aim of this study was to investigate the effect of insulin resistance on glycogen concentration and glycogen synthase activity in the red and white gastrocnemius muscles and to determine whether the inverse relationship existing between glycogen concentration and enzyme activity is maintained in insulin resistant ...

journal_title：Archives of biochemistry and biophysics

authors： Coderre L,Vallega GA,Pilch PF,Chipkin SR

更新日期：2007-08-01 00:00:00

abstract：:The delta pH in ionophore-treated cells of an acidophile has been determined by electron spin resonance spectroscopy. The values obtained were comparable to those obtained using the more conventional techniques involving radiolabeled probes. No binding of the spin-labeled probe was observed as determined by two indepe...

journal_title：Archives of biochemistry and biophysics

authors： McLaggan D,Belkin S,Packer L,Matin A

更新日期：1989-08-15 00:00:00

abstract：:The retinal pigment epithelium (RPE)-specific protein RPE65 is the major protein of the RPE microsomal membrane fraction. Though RPE65 lacks transmembrane domains or signal peptide, detergents are required for its maximally effective solubilization in isotonic buffers. However, in 0.75-1.0 M KCl, RPE65 is as soluble w...

journal_title：Archives of biochemistry and biophysics

authors： Tsilou E,Hamel CP,Yu S,Redmond TM

更新日期：1997-10-01 00:00:00

abstract：:Using specific testosterone 16 alpha-hydroxylase activity as the basis for selection of fractions during purification, the cytochrome P-450 ("I"-P-450(16)alpha) has been isolated from livers of phenobarbital-treated 129/J female mice [K. Devore, N. Harada, and M. Negishi (1985) Biochemistry, 24, 5632-5637]. An antibod...

journal_title：Archives of biochemistry and biophysics

authors： Noshiro M,Serabjit-Singh CJ,Bend JR,Negishi M

更新日期：1986-02-01 00:00:00

abstract：:NAD kinase from human neutrophils has been partially purified by sequential application of Red Agarose, ion-exchange, and gel-filtration chromatography. The enzyme has a broad pH optimum, 7.0-9.5, is strictly dependent upon the presence of Mg2+, and in the absence of calcium exhibits Km values of 0.6 and 0.9 mM for NA...

journal_title：Archives of biochemistry and biophysics

authors： Williams MB,Jones HP

更新日期：1985-02-15 00:00:00

abstract：:Exposure of MCF-7 breast cancer cells to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) causes an elevated cytochrome P450 content and a marked increase in the microsomal hydroxylation of 17 beta-estradiol (E2) at the C-2, C-4, C-15 alpha, and C-6 alpha positions. In this study we investigated the involvement of cytochrom...

journal_title：Archives of biochemistry and biophysics

authors： Spink DC,Eugster HP,Lincoln DW 2nd,Schuetz JD,Schuetz EG,Johnson JA,Kaminsky LS,Gierthy JF

更新日期：1992-03-01 00:00:00

abstract：:Modulation of CYP3A1 and CYP3A2 mRNA expression by dexamethasone and by phenobarbital has been studied in immature (21-day-old) and adult (90-day-old) rat liver. Positive modulation of these forms by both agents markedly declines with the age of the animals. However, CYP3A2 mRNA, although physiologically extinguished ...

journal_title：Archives of biochemistry and biophysics

authors： Telhada MB,Pereira TM,Lechner MC

更新日期：1992-11-01 00:00:00

abstract：:Numerous studies on the application of low temperature plasma (LTP) have produced impressive results, including antimicrobial, antitumor, and wound healing effects. Although LTP research has branched out to include medical applications, the detailed effects and working mechanisms of LTP on wound healing have not been ...

journal_title：Archives of biochemistry and biophysics

authors： Cui HS,Joo SY,Lee DH,Yu JH,Jeong JH,Kim JB,Seo CH

更新日期：2017-09-15 00:00:00

abstract：:Upon treatment of Papaveracea cells with fungal elicitors, the biosynthesis of benzo[c]phenanthridine alkaloids is induced. Dihydrobenzophenanthridine oxidase, which catalyzes a later step in the biogenesis of these alkaloids, is one of the enzymes whose activity is elevated in the process. Here we report the 211-fold...

journal_title：Archives of biochemistry and biophysics

authors： Arakawa H,Clark WG,Psenak M,Coscia CJ

更新日期：1992-11-15 00:00:00

abstract：:Glycosaminoglycan (GAG)-protein interactions regulate a myriad of physiologic and pathologic processes, yet an understanding of how these molecules interact is lacking. The role of the pattern and spacing of basic amino acids (arginine (R) and lysine (K)) in heparin binding sites was investigated using peptide analogs...

journal_title：Archives of biochemistry and biophysics

authors： Fromm JR,Hileman RE,Caldwell EE,Weiler JM,Linhardt RJ

更新日期：1997-07-01 00:00:00

abstract：:The acute effect of ethanol on hepatic protein synthesis is a rather controversial issue. In view of the conflicting reports on this subject, the effect of ethanol on protein labeling from L-[3H]valine in isolated liver cells was studied under a variety of experimental conditions. When tracer doses of the isotope were...

journal_title：Archives of biochemistry and biophysics

authors： Girbes T,Susin A,Ayuso MS,Parrilla R

更新日期：1983-10-01 00:00:00

abstract：:The reaction center of photosystem 2 has been highly purified from digitonin-solubilized thylakoid membranes of the thermophilic cyanobacterium Synechococcus sp. by means of sucrose density gradient centrifugation and electrophoresis on polyacrylamide gels containing digitonin. Sodium dodecyl sulfate-polyacrylamide ge...

journal_title：Archives of biochemistry and biophysics

authors： Yamagishi A,Katoh S

更新日期：1983-09-01 00:00:00

abstract：:An improved high yielding procedure for the purification of myrosinase from Sinapis alba L. consisting of concanavalin A affinity chromatography followed by a chromatofocusing step is presented. The purified enzyme was homogeneous as judged by sodium dodecyl sulfate-gel electrophoresis and by analytical ultracentrifug...

journal_title：Archives of biochemistry and biophysics

authors： Pessina A,Thomas RM,Palmieri S,Luisi PL

更新日期：1990-08-01 00:00:00

abstract：:Differential scanning calorimetry measures the heat capacity of states and the excess heat associated with transitions that can be induced by temperature change. The integral of the excess heat capacity is the enthalpy for this process. Despite this potentially intimidating sounding physical chemistry background, DSC ...

journal_title：Archives of biochemistry and biophysics

authors： Johnson CM

更新日期：2013-03-01 00:00:00

abstract：:The 3-dimensional structure determination of rat phenylalanine hydroxylase (PAH) has identified potentially important amino acids lining the active site cleft with the majority of these having hydrophobic side-chains including several with aromatic side chains. Here we have analyzed the effect on rat PAH enzyme kineti...

journal_title：Archives of biochemistry and biophysics

authors： Jennings IG,Cotton RG,Kobe B

更新日期：2000-12-15 00:00:00

abstract：:Earlier findings on electronitration of hen egg-white lysozyme demonstrated a product which was mononitrated at Tyr23, by ion-exchange chromatography, absorbance at 430 nm, dithionite reduction, and Edman sequencing of a nitrated proteolytic peptide. However, the whole protein was not sequenced; therefore, although th...

journal_title：Archives of biochemistry and biophysics

authors： Kendall G,Cooper HJ,Heptinstall J,Derrick PJ,Walton DJ,Peterson IR

更新日期：2001-08-15 00:00:00