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Electron spin resonance measurements of the effect of ionophores on the transmembrane pH gradient of an acidophilic bacterium.

Abstract：

:The delta pH in ionophore-treated cells of an acidophile has been determined by electron spin resonance spectroscopy. The values obtained were comparable to those obtained using the more conventional techniques involving radiolabeled probes. No binding of the spin-labeled probe was observed as determined by two independent control experiments and by the characteristics of the probe signal. These results led us to conclude that the delta pH measured in protonophore/ionophore-treated cells is a result of a Donnan potential, which may be a physical property of all intact bacterial cells at low pH values.

journal_name

Arch Biochem Biophys

journal_title

Archives of biochemistry and biophysics

authors

McLaggan D,Belkin S,Packer L,Matin A

doi

10.1016/0003-9861(89)90180-x

subject

Has Abstract

pub_date

1989-08-15 00:00:00

pages

206-14

issue

1

eissn

0003-9861

issn

1096-0384

pii

0003-9861(89)90180-X

journal_volume

273

pub_type

杂志文章

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