Structural, functional and unfolding characteristics of glutathione S-transferase of Plasmodium vivax.

abstract：:Infrared spectroscopy was used to investigate the changes in bands assigned to phospholipids and proteins in dehydrated and rehydrated sarcoplasmic reticulum. The changes in CH2 and CH3 stretching bands, amide bands, and phosphate stretching bands are similar to shifts in frequency seen for those bands in phospholipid...

journal_title：Archives of biochemistry and biophysics

authors： Crowe JH,Crowe LM,Chapman D

更新日期：1984-07-01 00:00:00

abstract：:G protein-coupled receptors can be directly modulated by changes in transmembrane voltage in a variety of cell types. Here we show that, while changes in the membrane voltage itself do not induce detectable modifications in the cytosolic Ca(2+) concentration, platelet stimulation with thrombin or the PAR-1 and PAR-4 a...

journal_title：Archives of biochemistry and biophysics

authors： Albarrán L,Dionisio N,López E,Salido GM,Rosado JA

更新日期：2013-10-15 00:00:00

abstract：:If liver alcohol dehydrogenase were rate-limiting in ethanol metabolism, inhibitors of the enzyme should inhibit the metabolism with the same type of kinetics and the same kinetic constants in vitro and in vivo. Against varied concentrations of ethanol, 4-methylpyrazole is a competitive inhibitor of purified rat liver...

journal_title：Archives of biochemistry and biophysics

authors： Plapp BV,Leidal KG,Smith RK,Murch BP

更新日期：1984-04-01 00:00:00

abstract：:A series of esters of adenosine 5'-monophosphate with ethyl, propyl, or hexyl moieties substituted at the omega-position with chlorine or bromine were prepared. The compounds were competitive inhibitors of horse liver alcohol dehydrogenase with respect to coenzyme, NAD+, and had inhibition (dissociation) constants in ...

journal_title：Archives of biochemistry and biophysics

authors： Fries RW,Bohlken DP,Murch BP,Leidal KG,Plapp BV

更新日期：1983-08-01 00:00:00

abstract：:By anion-exchange chromatography, the nitrogen fixing photosynthetic stem nodules and nonphotosynthetic root nodules of Sesbania rostrata are shown to contain the same seven major components of leghemoglobin (Lb), numbered LbI-LbVII in order of elution, although in different proportions. No novel component was found i...

journal_title：Archives of biochemistry and biophysics

authors： Bogusz D,Kortt AA,Appleby CA

更新日期：1987-04-01 00:00:00

abstract：:High-performance size exclusion chromatography revealed that alkaline-extracted peach fruit pectin dissolved in 0.05 M NaNO3 comprised a hierarchy of at least four aggregated, macromolecular-sized species. Each of the three largest species was found to be comparable in length to the three smallest subunits of an inter...

journal_title：Archives of biochemistry and biophysics

authors： Fishman ML,Cooke P,Levaj B,Gillespie DT,Sondey SM,Scorza R

更新日期：1992-04-01 00:00:00

abstract：:Using the polymerase chain reaction (PCR) a 0.8-kb product was amplified from cDNA made from the midgut and Malpighian tubules of fifth instar larvae of Helicoverpa virescens. This 0.8-kb PCR product was then used to isolate a clone of the B subunit of the V-type ATPase from a cDNA library made from the same tissues. ...

journal_title：Archives of biochemistry and biophysics

authors： Gill SS,Ross LS

更新日期：1991-11-15 00:00:00

abstract：:A novel series of nonpeptidic compounds structurally related to the known anticholesteremic thyropropic acid were found to inhibit Escherichia coli peptide deformylase (PDF), with IC50 values in the low-micromolar range. Kinetic analysis of [4-(4-hydroxyphenoxy)-3,5-diiodophenyl]acetic acid reveals competitive inhibit...

journal_title：Archives of biochemistry and biophysics

authors： Jayasekera MM,Kendall A,Shammas R,Dermyer M,Tomala M,Shapiro MA,Holler TP

更新日期：2000-09-15 00:00:00

abstract：:The effects of in vitro addition of halogenated hydrocarbons on the susceptibility of various rat tissues to lipid peroxidation, and of iron overload and dietary vitamin E in the intact rat on subsequent lipid peroxidation in rat tissue slices were examined. The ease and speed of tissue slice preparation allowed testi...

journal_title：Archives of biochemistry and biophysics

authors： Gavino VC,Dillard CJ,Tappel AL

更新日期：1984-09-01 00:00:00

abstract：:The reaction by which the antioxidant capacity of alpha-tocopherol is sustained by recycling of the alpha-tocopheroxyl radical in membranes or lipoproteins by aqueous ascorbate has been studied by laser flash photolysis in model micellar and membrane systems. In bilayers of dimyristoylphosphatidylcholine at 35 degrees...

journal_title：Archives of biochemistry and biophysics

authors： Bisby RH,Parker AW

更新日期：1995-02-20 00:00:00

abstract：:Human leukemic T-lymphocytes undergo extensive and rapid apoptosis in the presence of L1AD3, a small cyclic peptide derivative of cobra cardiotoxin. The first step in this process involves its binding to membranes of susceptible cells. By the use of a biotin "handle" synthetically incorporated at the N-terminus of L1A...

journal_title：Archives of biochemistry and biophysics

authors： Smith CA,Hinman CL

更新日期：2004-12-01 00:00:00

abstract：:The mechanism of the biosynthesis of 12-oxo-10,15(Z)-phytodienoic acid (12-oxo-PDA) from 13(S)-hydroperoxy-9(Z),11(E),15(Z)-octadecatrienoic acid in preparations of corn (Zea mays L.) was studied. In the initial reaction the hydroperoxide was converted into an unstable allene oxide, 12,13(S)-epoxy-9(Z),11,15(Z)-octade...

journal_title：Archives of biochemistry and biophysics

authors： Hamberg M,Fahlstadius P

更新日期：1990-02-01 00:00:00

abstract：:In the Escherichia coli class Ia ribonucleotide reductase (RNR), the best characterized RNR, there is no spectroscopic evidence for the existence of the postulated catalytically essential thiyl radical (R-S(*)) in the substrate binding subunit R1. We report first results on artificially generated thiyl radicals in R1 ...

journal_title：Archives of biochemistry and biophysics

authors： Kolberg M,Bleifuss G,Sjöberg BM,Gräslund A,Lubitz W,Lendzian F,Lassmann G

更新日期：2002-01-01 00:00:00

abstract：:delta 2-Isopentenylpyrophosphate:5'AMP delta 2-isopentenyltransferase, which catalyzes the formation of isopentenyl-AMP from delta 2-isopentenylpyrophosphate and 5'AMP, was purified 6800-fold from the fruiting body of the cellular slime mold Dictyostelium discoideum using several separation procedures including 5'AMPo...

journal_title：Archives of biochemistry and biophysics

authors： Ihara M,Taya Y,Nishimura S,Tanaka Y

更新日期：1984-05-01 00:00:00

abstract：:A numerical method was applied to a system of differential rate equations describing the monomer-dimer-inhibitor (M-D-I) interaction involving human immunodeficiency virus type 1 protease and a peptidomimetic, competitive inhibitor. Two pairs of progress curves were obtained, one involving the M-D interaction and the ...

journal_title：Archives of biochemistry and biophysics

authors： Morelock MM,Graham ET,Erdman D,Pargellis CA

更新日期：1996-04-15 00:00:00

abstract：:A novel halophilic, alkalithermostable lipase LipR2 from Alkalispirillum sp. NM-ROO2 was cloned and expressed. LipR2 was covalently immobilized on Florisil® functionalized with glutaraldehyde. Protein binding efficiency of functionalized Florisil® was 94.7%. Immobilized LipR2 retained 97.5% of specific activity of the...

journal_title：Archives of biochemistry and biophysics

authors： Mesbah NM

更新日期：2019-05-30 00:00:00

abstract：:The ferroxidase Fet3 and the permease Ftr1 constitute a well-conserved high-affinity iron uptake system in yeast. We have investigated the mechanism of transcriptional regulation of Fet3 in the methylotrophic yeast Pichia pastoris. Isolation and functional analysis of the Fet3 promoter indicate that a GATA sequence el...

journal_title：Archives of biochemistry and biophysics

authors： Miele R,Barra D,Bonaccorsi di Patti MC

更新日期：2007-09-01 00:00:00

abstract：:The enzyme phenylalanine hydroxylase catalyzes the hydroxylation of excess phenylalanine in the liver to tyrosine. The enzyme is regulated allosterically by phenylalanine and by phosphorylation of Ser16. Hydrogen/deuterium exchange monitored by mass spectrometry has been used to gain insight into any structural change...

journal_title：Archives of biochemistry and biophysics

authors： Li J,Fitzpatrick PF

更新日期：2013-07-15 00:00:00

abstract：:Geranylgeranyl diphosphate synthase supplies the essential acyclic precursor for Taxol biosynthesis in methyl jasmonate-induced Taxus canadensis suspension cell cultures. A cDNA encoding this prenyltransferase was cloned from an induced T. canadensis cell library. The recombinant enzyme expressed in yeast was confirme...

journal_title：Archives of biochemistry and biophysics

authors： Hefner J,Ketchum RE,Croteau R

更新日期：1998-12-01 00:00:00

abstract：:Because angiogenesis underlies the pathogenesis of numerous conditions (cancer, psoriasis, macular degeneration), there is a pressing need for continued investigations into angiogenic signaling and potential drug targets. Antiangiogenic agents can be classified as either direct or indirect. Direct antiangiogenics act ...

journal_title：Archives of biochemistry and biophysics

authors： Berrios RL,Arbiser JL

更新日期：2011-04-15 00:00:00

abstract：:Antisera against peptides from the extreme N- and C-terminal regions of the VDR were evaluated. The N-terminal antiserum Ab192 functioned as a general-purpose antibody, being able to supershift the rhVDR in heterodimeric or homodimeric binding complexes in the EMSA, and detect both recombinant and native forms of the ...

journal_title：Archives of biochemistry and biophysics

authors： Koszewski NJ,Reinhardt TA,Langub MC,Malluche HH,Horst RL

更新日期：1998-01-15 00:00:00

abstract：:Tyr-503 of beta-galactosidase was specifically replaced with Phe, His, Cys, and Lys using site-directed mutagenesis. The normal enzyme and the substituted enzymes were purified. The activities of each of the substituted enzymes with o-nitrophenyl-beta-D-galactopyranoside (ONPG) and p-nitrophenyl-beta-D-galactopyronosi...

journal_title：Archives of biochemistry and biophysics

authors： Ring M,Huber RE

更新日期：1990-12-01 00:00:00

abstract：:Novel vitamin D3 derivatives, 26-homo-delta 22-1 alpha,25(S)-dihydroxyvitamin D3 and 26-homo-delta 22-1 alpha,25(R)-dihydroxyvitamin D3 were compared with the native hormone, 1,25-dihydroxyvitamin D3, and with other vitamin D3 derivatives, in inhibition of cell growth, induction of phenotypic differentiation, and c-my...

journal_title：Archives of biochemistry and biophysics

authors： Koizumi T,Nakao Y,Ishizuka S,Oshida J,Hara N,Ikekawa N,Fijita T

更新日期：1990-02-01 00:00:00

abstract：:We investigated the role of free radicals in hemoglobin (Hb) oxidation and denaturation. To generate free radicals, we used two azocompounds, the hydrophilic 2,2'-azobis(2-amidinopropane hydrochloride and the hydrophobic 2,2'-azobis(2,4-dimethylvaleronitrile) and a drug of the quinone family, phenazine methosulfate. T...

journal_title：Archives of biochemistry and biophysics

authors： Minetti M,Mallozzi C,Scorza G,Scott MD,Kuypers FA,Lubin BH

更新日期：1993-04-01 00:00:00

abstract：:To establish a cell line expressing enhanced levels of beta-amyloid precursor protein (beta-APP), we constructed plasmid DNAs expressing beta-APP-751 mRNA and transfected them into COS-1 cells. Using a modified version of the reverse transcriptase polymerase chain reaction which is RNA sensitive to study the beta-APP ...

journal_title：Archives of biochemistry and biophysics

authors： Ramakrishna N,Smedman M,Gillam B

更新日期：1996-02-15 00:00:00

abstract：:The plant-derived flavonoid, quercetin (QCT), has many biological actions, including cardioprotective actions, resulting from its antioxidant and anti-inflammatory effects. In this study, effects of QCT and its metabolites on the contraction and Ca2+ transients (CaT) of mouse single cardiomyocytes were simultaneously ...

journal_title：Archives of biochemistry and biophysics

authors： Hayamizu K,Morimoto S,Nonaka M,Hoka S,Sasaguri T

更新日期：2018-01-01 00:00:00

abstract：:Glutamine transport in both normal and acidotic rat kidney mitochondria was investigated using both isotopic techniques and by spectroscopic measurements in which glutamine metabolism was allowed to occur. Widely used criteria for demonstrating the occurrence of carrier-mediated transport were successfully applied in ...

journal_title：Archives of biochemistry and biophysics

authors： Atlante A,Passarella S,Minervini GM,Quagliariello E

更新日期：1994-12-01 00:00:00

abstract：:OmpD is the major Salmonella enterica serovar Typhimurium (S. Typhimurium) porin and mediates hydrogen peroxide (H2O2) influx. The results described herein extend this finding to hypochlorous acid (HOCl), another reactive oxygen species that is also part of the oxidative burst generated by the phagosome. S. Typhimuriu...

journal_title：Archives of biochemistry and biophysics

authors： Aguayo D,Pacheco N,Morales EH,Collao B,Luraschi R,Cabezas C,Calderón P,González-Nilo F,Gil F,Calderón IL,Saavedra CP

更新日期：2015-02-15 00:00:00

abstract：:The plasma membrane of cells contains enzymes whose active sites face the external medium rather than the cytoplasm. The activities of these enzymes, referred to as ectoenzymes, can be measured using living cells. In this work we describe the ability of living promastigotes of Leishmania amazonensis to hydrolyze extra...

journal_title：Archives of biochemistry and biophysics

authors： Berrêdo-Pinho M,Peres-Sampaio CE,Chrispim PP,Belmont-Firpo R,Lemos AP,Martiny A,Vannier-Santos MA,Meyer-Fernandes JR

更新日期：2001-07-01 00:00:00

abstract：:The excimer fluorescence of pyrene iodoacetamide-labeled tropomyosin bound to actin is a probe of the regulatory state change of the muscle thin filament associated with the cooperative binding of myosin subfragment 1 (S1) (Y. Ishii and S. S. Lehrer, Biochemistry 29, 1160, 1990). In this study, the excimer fluorescenc...

journal_title：Archives of biochemistry and biophysics

authors： Ishii Y,Lehrer SS

更新日期：1993-08-15 00:00:00