当前位置： SCI文献检索 > ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS期刊下所有文献 > Purification and some properties of delta 2-isopentenylpyrophosphate:5'AMP delta 2-isopentenyltransferase from the cellular slime mold Dictyostelium discoideum.

Purification and some properties of delta 2-isopentenylpyrophosphate:5'AMP delta 2-isopentenyltransferase from the cellular slime mold Dictyostelium discoideum.

Abstract：

:delta 2-Isopentenylpyrophosphate:5'AMP delta 2-isopentenyltransferase, which catalyzes the formation of isopentenyl-AMP from delta 2-isopentenylpyrophosphate and 5'AMP, was purified 6800-fold from the fruiting body of the cellular slime mold Dictyostelium discoideum using several separation procedures including 5'AMPox-red AH-Sepharose 4B affinity column chromatography. The final preparation was very unstable and lost its activity in a day. Various properties of the 1000-fold-purified enzyme preparation were examined. The molecular mass was 40,000 +/- 2000 Da, as determined by Sephadex G-100 superfine gel filtration. The divalent metal ions Mn2+, Zn2+, and Mg2+ profoundly affected the enzymatic activity depending on their concentration, and also altered the optimum pH and temperature. Of the compounds tested, 5'AMP was the best acceptor of the isopentenyl group and, interestingly, ADP also served as a substrates, being 60-80% as effective as 5'AMP. Adenine, adenosine, and ATP were not substrates for this enzyme. Under the optimum assay conditions (pH 7.0, 1 mM Zn2+, and 25 degrees C) the Km values for 5'AMP and delta 2-isopentenylpyrophosphate were 1.0 X 10(-7)M and 2.2 X 10(-6)M, respectively.

journal_name

Arch Biochem Biophys

journal_title

Archives of biochemistry and biophysics

authors

Ihara M,Taya Y,Nishimura S,Tanaka Y

doi

10.1016/0003-9861(84)90446-6

subject

Has Abstract

pub_date

1984-05-01 00:00:00

pages

652-60

issue

2

eissn

0003-9861

issn

1096-0384

pii

0003-9861(84)90446-6

journal_volume

230

pub_type

杂志文章

相关文献

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS文献大全
  • Protein oxidation and aging. II. Difficulties in measuring alkaline protease activity in tissues using the fluorescamine procedure.

    abstract：:A current hypothesis explaining the aging process implicates the accumulation of oxidized protein in animal tissues. This is primarily based on a series of reports showing an age-dependent increase in protein carbonyl content and an age-dependent decrease in the activities of enzymes, especially of alkaline proteases,...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1995.1359

    authors： Cao G,Cutler RG

    更新日期：1995-06-20 00:00:00

  • Regulation of glycan processing by Golgi enzymes from red kidney bean (Phaseolus vulgaris) seedlings.

    abstract：:The effect of a protein matrix on the processing of glycoprotein glycans by Golgi enzymes from plant seedlings has been determined with an artificial glycoprotein system, comparing the processing rates of glycan-(biotinyl)Asn (or glycan-(biotinamidohexanoyl)Asn) substrates either free or bound to avidin. An analysis o...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(91)90020-j

    authors： Lu Y,Wold F

    更新日期：1991-04-01 00:00:00

  • Structure and heme-binding properties of HemQ (chlorite dismutase-like protein) from Listeria monocytogenes.

    abstract：:Chlorite dismutase-like proteins are structurally closely related to functional chlorite dismutases which are heme b-dependent oxidoreductases capable of reducing chlorite to chloride with simultaneous production of dioxygen. Chlorite dismutase-like proteins are incapable of performing this reaction and their biologic...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2015.01.010

    authors： Hofbauer S,Hagmüller A,Schaffner I,Mlynek G,Krutzler M,Stadlmayr G,Pirker KF,Obinger C,Daims H,Djinović-Carugo K,Furtmüller PG

    更新日期：2015-05-15 00:00:00

  • Bactericidal activity and poly-L-proline II conformation of the tandem repeat sequence of human salivary mucin glycoprotein (MG2).

    abstract：:The tandem repeat 23-residue sequence [TRS23 (145-167): T-T-A-A-P-P-T-P-S-A-T-T-P-A-P-P-S-S-S-A-P-P-E] of human salivary mucin glycoprotein MG2 was examined for its in vitro bactericidal activity against four oral microorganisms, Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Streptococcus gordonii, a...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1998.0779

    authors： Antonyraj KJ,Karunakaran T,Raj PA

    更新日期：1998-08-15 00:00:00

  • Effects of fluorescein isothiocyanate on insulin actions in rat adipocytes.

    abstract：:The effects of fluorescein isothiocyanate II (FITC) on the actions of insulin in rat adipocytes were studied. When adipocytes were incubated with FITC at pH 7.4 (2 mM agent, 8 min), the cells were completely deprived of their specific insulin-binding activity and rendered unresponsive to the hormone. The effect of FIT...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(92)90389-e

    authors： Goto Y,Sumida Y,Flanagan JE,Robinson FW,Simpson IA,Cushman SW,Kono T

    更新日期：1992-03-01 00:00:00

  • Mechanistic analysis of Mycobacterium tuberculosis Rv1347c, a lysine Nepsilon-acyltransferase involved in mycobactin biosynthesis.

    abstract：:Mycobactin acylation plays a crucial role in the ability of Mycobacterium tuberculosis to acquire intracellular iron during infection. M. tuberculosis Rv1347c, the lysine N(epsilon)-acyltransferase responsible for mycobactin acylation, represents a valid target for the development of novel anti-tubercular agents. Here...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2008.05.013

    authors： Frankel BA,Blanchard JS

    更新日期：2008-09-15 00:00:00

  • Protein denaturation during freezing and thawing in phosphate buffer systems: monomeric and tetrameric beta-galactosidase.

    abstract：:During freezing in sodium and potassium phosphate (NaP and KP) buffer solutions, changes in pH may impact the stability of proteins. Since the degradation pathways for the model proteins, monomeric and tetrameric beta-galactosidase (beta-gal), chosen for this study are governed by conformational changes (i.e., physica...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.2000.2088

    authors： Pikal-Cleland KA,Rodríguez-Hornedo N,Amidon GL,Carpenter JF

    更新日期：2000-12-15 00:00:00

  • Role of ADP ribosylation factor6- Cytohesin1-PhospholipaseD signaling axis in U46619 induced activation of NADPH oxidase in pulmonary artery smooth muscle cell membrane.

    abstract：:Treatment of human pulmonary artery smooth muscle cells (HPASMCs) with the thromboxane A2 receptor antagonist, SQ29548 inhibited U46619 stimulation of phospholipase D (PLD) and NADPH oxidase activities in the cell membrane. Pretreatment with apocynin inhibited U46619 induced increase in NADPH oxidase activity. The cel...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2017.08.012

    authors： Chakraborti S,Sarkar J,Chowdhury A,Chakraborti T

    更新日期：2017-11-01 00:00:00

  • Bacterial expression of functional membrane-bound thromboxane synthase having intact sequence and truncated N-terminal hydrophobic segment.

    abstract：:A full-length cDNA for human placental thromboxane synthase and a shortened cDNA lacking the sequence corresponding to the N-terminal 2-29 amino acids were expressed in Escherichia coli using a pCW expression vector. Both intact and truncated recombinant enzyme were found in the membrane fraction and were catalyticall...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1995.1427

    authors： Xia Z,Tai HH

    更新日期：1995-08-20 00:00:00

  • Accumulation of various N-acylethanolamines including N-arachidonoylethanolamine (anandamide) in cadmium chloride-administered rat testis.

    abstract：:Changes in the levels of various molecular species of N-acylethanolamine in CdCl2-administered rat testis were examined. We found that the levels of various N-acylethanolamines including anandamide (N-arachidonoylethanolamine), an endogenous cannabinoid receptor ligand, were dramatically increased in CdCl2-admin-ister...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1998.0688

    authors： Kondo S,Sugiura T,Kodaka T,Kudo N,Waku K,Tokumura A

    更新日期：1998-06-15 00:00:00

  • Selective effect of nerve growth factor on some Golgi and lysosomal enzyme activities of rat pheochromocytoma (PC12) cells.

    abstract：:Treatment with neuronal growth factor (NGF) results in the growth of neuronal processes by PC12 cells and a concomitant 70% increase in the area of the Golgi apparatus. To define the observed morphologic changes in biochemical terms, we investigated the effect of NGF treatment on some Golgi and lysosomal enzyme activi...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(89)90208-7

    authors： Rhodes CH,Mezitis SG,Gonatas NK,Fleischer B

    更新日期：1989-07-01 00:00:00

  • Suppression of Alzheimer amyloid precursor protein (APP) expression by exogenous APP mRNA.

    abstract：:To establish a cell line expressing enhanced levels of beta-amyloid precursor protein (beta-APP), we constructed plasmid DNAs expressing beta-APP-751 mRNA and transfected them into COS-1 cells. Using a modified version of the reverse transcriptase polymerase chain reaction which is RNA sensitive to study the beta-APP ...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1996.0072

    authors： Ramakrishna N,Smedman M,Gillam B

    更新日期：1996-02-15 00:00:00

  • Production and characterization of the functional fragment of pneumococcal surface protein A.

    abstract：:Pneumococcal surface protein A (PspA) is present on the cell wall of Streptococcus pneumoniae pathogen and has an antigenetically variable N-terminal domain. This aminoterminal domain is essential for full pneumococcal virulence, and monoclonal antibodies raised against it protect mice against pneumococcal infections....

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1999.1544

    authors： Jedrzejas MJ,Hollingshead SK,Lebowitz J,Chantalat L,Briles DE,Lamani E

    更新日期：2000-01-01 00:00:00

  • Recombinant human cytochrome P450 1A2 and an N-terminal-truncated form: construction, purification, aggregation properties, and interactions with flavodoxin, ferredoxin, and NADPH-cytochrome P450 reductase.

    abstract：:Previous work from this laboratory indicated that the N-terminus of recombinant human cytochrome P450 (P450) 1A2 expressed in Escherichia coli is blocked (P. Sandhu, Z. Guo, T. Baba, M. V. Martin, R. H. Tukey, and F. P. Guengerich, (1994) Arch. Biochem. Biophys. 30, 168 -177). A modification of this construct was done...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1996.0086

    authors： Dong MS,Yamazaki H,Guo Z,Guengerich FP

    更新日期：1996-03-01 00:00:00

  • Dermatan is a better substrate for 4-O-sulfation than chondroitin: implications in the generation of 4-O-sulfated, L-iduronate-rich galactosaminoglycans.

    abstract：:The biosynthesis of dermatan sulfate is a complex process that involves, inter alia, formation of L-iduronic acid residues by C5-epimerization of D-glucuronic acid residues already incorporated into the growing polymer. It has been shown previously that this reaction is promoted by the presence of the sulfate donor 3'...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.2000.2043

    authors： Eklund E,Rodén L,Malmström M,Malmström A

    更新日期：2000-11-15 00:00:00

  • The effect of ATP concentration on the rate of actin polymerization.

    abstract：:It was found that the rate of polymerization of G-actin increased with the decrease of ATP concentration. When excess ATP was replaced by chloride through anion-exchange treatment, the extent of actin polymerization did not change provided that the ionic strength was raised immediately after the treatment. In the mean...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(83)90426-5

    authors： Fung BM,Eyob E

    更新日期：1983-02-01 00:00:00

  • Binding and crossing: Methods for the characterization of membrane-active peptides interactions with membranes at the molecular level.

    abstract：:Antimicrobial and cell-penetrating peptides have been the object of extensive studies for more than 60 years. Initially these two families were studied separately, and more recently parallels have been drawn. These studies have given rise to numerous methodological developments both in terms of observation techniques ...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章,评审

    doi：10.1016/j.abb.2021.108751

    authors： Emmanuelle S,Astrid W,Sandrine S,Sophie C,Nicolas R

    更新日期：2021-01-07 00:00:00

  • Inhibition of peroxidase-catalyzed reactions by deferoxamine.

    abstract：:Phagocytes generate superoxide (O2-.) and hydrogen peroxide (H2O2) and their interaction in an iron-catalyzed reaction to form hydroxyl radicals (OH.) (Haber-Weiss reaction) has been proposed. Deferoxamine chelates iron in a catalytically inactive form, and thus inhibition by deferoxamine has been employed as evidence...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(88)90326-8

    authors： Klebanoff SJ,Waltersdorph AM

    更新日期：1988-08-01 00:00:00

  • Modification of an essential amino group of phosphoenolpyruvate carboxylase from maize leaves by pyridoxal phosphate and by pyridoxal phosphate-sensitized photooxidation.

    abstract：:Phosphoenolpyruvate carboxylase from maize leaves was inactivated by pyridoxal 5'-phosphate in the dark and in the light. A two-step reversible mechanism is proposed for inactivation in the dark, which involves the formation of a noncovalent complex prior to a Schiff base with amino groups of the enzyme. Spectral anal...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(86)90309-7

    authors： Podesta FE,Iglesias AA,Andreo CS

    更新日期：1986-05-01 00:00:00

  • Characterization of the pH titration shifts of ribonuclease A by one- and two-dimensional nuclear magnetic resonance spectroscopy.

    abstract：:One- and two-dimensional 1H NMR experiments were used to determine the chemical shifts of resonances arising from 29 residues of RNase A in H2O at 17 pH values ranging from 1.2 to 7.9 Nearly all resonances displaying pH-induced changes in chemical shift greater than 0.1 ppm were monitored. Individual plots of the chem...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1996.0108

    authors： Baker WR,Kintanar A

    更新日期：1996-03-01 00:00:00

  • Static compression is associated with decreased diffusivity of dextrans in cartilage explants.

    abstract：:The chondrocytes of adult articular cartilage rely upon transport phenomena within their avascular extracellular matrix for many biological activities. Therefore, changes in matrix structure which influence cytokine transport parameters may be an important mechanism involved in the chondrocyte response to tissue compr...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.2000.2077

    authors： Quinn TM,Kocian P,Meister JJ

    更新日期：2000-12-15 00:00:00

  • Superoxide produced by mitochondrial complex III plays a pivotal role in the execution of ferroptosis induced by cysteine starvation.

    abstract：:Ferroptosis is a type of iron-dependent, non-apoptotic cell death, which is typically induced by cysteine starvation or by the inhibition of glutathione peroxidase 4 (GPX4) activity with the accompanying elevation in lipid peroxidation product levels. Despite the central role of mitochondria in oxidative metabolism an...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2021.108775

    authors： Homma T,Kobayashi S,Sato H,Fujii J

    更新日期：2021-01-22 00:00:00

  • Efflux mechanism and pathway of verapamil pumping by human P-glycoprotein.

    abstract：:Multidrug resistance (MDR) caused by overexpressed permeability-glycoprotein (P-gp) in cancer cells is the main barrier for the cure of cancers. P-gp can pump many chemotherapeutic drugs, which is a viable target to overcome P-gp-mediated MDR by efficient inhibitors of P-gp. However, limited understanding of the efflu...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2020.108675

    authors： Wang L,Sun Y

    更新日期：2020-12-15 00:00:00

  • Effects of oxidants on properties of fluorescent calcium indicators.

    abstract：:An increasing number of studies use calcium-sensitive fluorescent dyes to address the relationship between elevated levels of intracellular calcium and free-radical-mediated damage in a variety of pathophysiological phenomena. The present study evaluates the effects of reactive oxygen species on the spectral propertie...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1997.0518

    authors： Sarvazyan N,Swift L,Martinez-Zaguilan R

    更新日期：1998-02-01 00:00:00

  • Recombinant human erythropoietin: purification and analysis of carbohydrate linkage.

    abstract：:Erythropoietin was purified to homogeneity from the culture medium of a baby hamster kidney cell line stably transfected with a human erythropoietin gene. A three-step procedure was used, which included affinity chromatography, ion-exchange chromatography, and reverse-phase chromatography. Purity of the protein was co...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(88)90135-x

    authors： Broudy VC,Tait JF,Powell JS

    更新日期：1988-09-01 00:00:00

  • Interflavin one-electron transfer in the inducible nitric oxide synthase reductase domain and NADPH-cytochrome P450 reductase.

    abstract：:In this study, we have analyzed interflavin electron transfer reactions from FAD to FMN in both the full-length inducible nitric oxide synthase (iNOS) and its reductase domain. Comparison is made with the interflavin electron transfer in NADPH-cytochrome P450 reductase (CPR). For the analysis of interflavin electron t...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2005.05.027

    authors： Yamamoto K,Kimura S,Shiro Y,Iyanagi T

    更新日期：2005-08-01 00:00:00

  • Effect of the phosphate substrate on drug-inhibitor binding to human purine nucleoside phosphorylase.

    abstract：:The thermodynamics of the drug-inhibitors acyclovir, ganciclovir, and 9-benzylguanine binding to human purine nucleoside phosphorylase (hsPNP) were determined from isothermal titration calorimetry as a function of the substrate phosphate ion (Pi) concentration from 0 to 0.125 M and temperature from 15 degrees C to 35 ...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2008.10.008

    authors： Todorova NA,Schwarz FP

    更新日期：2008-12-15 00:00:00

  • Purification and characterization of an acyclic monoterpene primary alcohol:NADP+ oxidoreductase from catmint (Nepeta racemosa).

    abstract：:A soluble monoterpene primary alcohol:NADP+ oxidoreductase has been purified to apparent homogeneity from leaves of the catmint, Nepeta racemosa. The purified enzyme consisted of two polypeptides, with molecular masses of 42,000 and 40,000 Da, and contained zinc ions. A number of monoterpene alcohols (geraniol, nerol,...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1995.1210

    authors： Hallahan DL,West JM,Wallsgrove RM,Smiley DW,Dawson GW,Pickett JA,Hamilton JG

    更新日期：1995-04-01 00:00:00

  • Purification and characterization of D-myo-inositol (1,4,5)/(1,3,4,5)- polyphosphate 5-phosphatase from skeletal muscle.

    abstract：:In this investigation we report the presence of two forms of inositol (1,4,5)P3/(1,3,4,5)P4-polyphosphate 5-phosphatase activity (types I and II) which were observed in soluble extracts of skeletal muscle after fractionation by DEAE-Sephacel chromatography. Hydrolysis of D-myo-inositol 1,4,5-trisphosphate (Ins(1,4,5)P...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1994.1207

    authors： Hansbro PM,Foster PS,Hogan SP,Ozaki S,Denborough MA

    更新日期：1994-05-15 00:00:00

  • Mechanism of the aromatic aminotransferase encoded by the Aro8 gene from Saccharomyces cerevisiae.

    abstract：:The amino acid L-lysine is synthesized in Saccharomyces cerevisiae via the α-aminoadipate pathway. An as yet unidentified PLP-containing aminotransferase is thought to catalyze the formation of α-aminoadipate from α-ketoadipate in the L-lysine biosynthetic pathway that could be the yeast Aro8 gene product. A screen of...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2011.09.008

    authors： Karsten WE,Reyes ZL,Bobyk KD,Cook PF,Chooback L

    更新日期：2011-12-01 00:00:00