Binding and crossing: Methods for the characterization of membrane-active peptides interactions with membranes at the molecular level.

abstract：:The oxygenation pattern of the essential oil monoterpenes of commercial mint (Mentha) species is determined by regiospecific cytochrome P450-catalyzed hydroxylation of the common olefinic precursor (-)-4S-limonene. In spearmint (M. spicata), C6-allylic hydroxylation leads to (-)-trans-carveol and thence (-)-carvone, w...

journal_title：Archives of biochemistry and biophysics

authors： Haudenschild C,Schalk M,Karp F,Croteau R

更新日期：2000-07-01 00:00:00

abstract：:Intracellular glutathione (GSH) depletion induced by buthionine sulfoximine (BSO) caused cell death that seemed to be apoptosis in C6 rat glioma cells. Arachidonic acid (AA) promoted BSO-induced cell death by accumulating reactive oxygen species (ROS) or hydroperoxides. AA inhibited caspase-3 activation and internucle...

journal_title：Archives of biochemistry and biophysics

authors： Higuchi Y,Yoshimoto T

更新日期：2002-04-01 00:00:00

abstract：:A gold(III) compound [Au(C^N^C)(IMe)]CF(3)SO(3) (Gold III) has been reported to have anticancer properties as it is able to reduce topoisomerase IB activity in vitro and suppress tumor growth in nude mice model. Here we have investigated the mechanism of inhibition of human topoisomerase IB activity by this compound, ...

journal_title：Archives of biochemistry and biophysics

authors： Castelli S,Vassallo O,Katkar P,Che CM,Sun RW,Desideri A

更新日期：2011-12-15 00:00:00

abstract：:The total lipid composition of highly purified plasma membranes from light-grown barley (Hordeum vulgare) leaves was investigated. The plasma membranes were separated from intracellular membranes by subfractionation of the microsomal fraction using aqueous polymer two-phase partitioning. A novel finding was that gluco...

journal_title：Archives of biochemistry and biophysics

authors： Rochester CP,Kjellbom P,Andersson B,Larsson C

更新日期：1987-06-01 00:00:00

abstract：:Preparative isoelectric focusing and gel filtration chromatography were used to purify a carboxypeptidase produced by the entomopathogenic fungus Metarhizium anisopliae during growth on cockroach cuticle. The enzyme was inhibited by diisopropyl fluorophosphate, implying involvement of a serine residue in catalysis. Ho...

journal_title：Archives of biochemistry and biophysics

authors： St Leger RJ,Bidochka MJ,Roberts DW

更新日期：1994-11-01 00:00:00

abstract：:Transcription factor Nkx2.5, essential for heart development, regulates cardiomyocyte-specific gene expression through combinatorial interactions with other cardiac-restricted (GATA4 and dHAND) or ubiquitous (p300) transcription regulators. Here we demonstrate that Nkx2.5 and p53 synergistically activate the promoter ...

journal_title：Archives of biochemistry and biophysics

authors： Kojic S,Nestorovic A,Rakicevic L,Protic O,Jasnic-Savovic J,Faulkner G,Radojkovic D

更新日期：2015-03-01 00:00:00

abstract：:The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that functions as an intracellular mediator in the xenobiotic signaling pathway. Although a number of studies have examined AhR-mediated CYP1A1 induction in detail, recent studies of AhR-null mice have revealed that AhR plays important regu...

journal_title：Archives of biochemistry and biophysics

authors： Kawajiri K,Fujii-Kuriyama Y

更新日期：2007-08-15 00:00:00

abstract：:Transforming growth factor-β (TGF-β) is pivotal in the pathogenesis of diabetic nephropathy. Type 1 TGF-β receptor (TGF-βR1) is degraded by Smad7-dependent ubiquitination-proteasomal pathway, which is deubiquitinated by ubiquitin C-terminal hydrolase-L5 (UCHL5). Therefore, we studied the role of UCHL5 in high glucose ...

journal_title：Archives of biochemistry and biophysics

authors： Ko YM,Chang CY,Chiou SJ,Hsu FJ,Huang JS,Yang YL,Guh JY,Chuang LY

更新日期：2013-07-15 00:00:00

abstract：:An inorganic pyrophosphatase (PPase) was purified from thylakoid membrane of spinach leaves to electrophoretic purity by methods including detergent solubilization, ammonium sulfate fractionation, and successive chromatographic techniques. Current protocol yielded about 10% recovery of total activity with a 30-fold pu...

journal_title：Archives of biochemistry and biophysics

authors： Jiang SS,Fan LL,Yang SJ,Kuo SY,Pan RL

更新日期：1997-10-01 00:00:00

abstract：:Myeloperoxidase (MPO) is a heme-containing peroxidase expressed mainly in neutrophils and to a lesser degree in monocytes. In the presence of hydrogen peroxide and halides, MPO catalyzes the formation of reactive oxygen intermediates, including hypochlorous acid (HOCl). The MPO/HOCl system plays an important role in m...

journal_title：Archives of biochemistry and biophysics

authors： Aratani Y

更新日期：2018-02-15 00:00:00

abstract：:No larger molecular weight component appeared in the high-performance gel chromatogram when calcium alone was added to the riboflavin-binding protein solution (RfBP), indicating that calcium alone did not aggregate it. RfBP bound calcium, but the amount of bound calcium decreased markedly upon dephosphorylation. Cross...

journal_title：Archives of biochemistry and biophysics

authors： Aoki T,Yamao Y,Yonemasu E,Kumasaki Y,Kako Y

更新日期：1993-09-01 00:00:00

abstract：:The Mr 245,000 calmodulin-binding protein of the dogfish erythrocyte cytoskeleton (D245) has been compared with human erythrocyte spectrin and mammalian brain fodrin [J. Levine and M. Willard (1981) J. Cell Biol. 90, 631-643]. Mammalian erythrocyte alpha-spectrin, brain alpha-fodrin, and D245 are all localized in the ...

journal_title：Archives of biochemistry and biophysics

authors： Bartelt DC,Carlin RK,Scheele GA,Cohen WD

更新日期：1984-04-01 00:00:00

abstract：:In order to characterize cellular retinoic acid-binding protein (CRABP) from chick embryos, we resolved the [3H]RA-binding activity of embryo extracts by Mono Q anion-exchange chromatography. Fractions containing [3H]RA-binding proteins eluted in two peaks at 7 and 10 min with the latter showing 10-fold higher activit...

journal_title：Archives of biochemistry and biophysics

authors： Venepally P,Reddy LG,Sani BP

更新日期：1996-12-15 00:00:00

abstract：:A protocol for dry weight determination of the concentration of protein, using 0.2-1.0 mg of protein per sample, has been presented and applied to nine proteins: bovine serum albumin, ovalbumin, bovine carbonic anhydrase B, galactoside binding protein (rabbit), lens calinaris lectin B, green pea lectin, soy bean agglu...

journal_title：Archives of biochemistry and biophysics

authors： Nozaki Y

更新日期：1986-09-01 00:00:00

abstract：:The actions of polycyclic aromatic hydrocarbons and glucocorticoids to regulate the expression of cytochrome P450c were investigated using cultured fetal rat hepatocytes. Cytochrome P450c mRNA content, determined by Northern blot analysis, was induced in cells treated with 1,2-benzanthracene from levels undetectable i...

journal_title：Archives of biochemistry and biophysics

authors： Mathis JM,Houser WH,Bresnick E,Cidlowski JA,Hines RN,Prough RA,Simpson ER

更新日期：1989-02-15 00:00:00

abstract：:Peroxisome proliferator-activated receptor alpha (PPARα) has been implicated in the pathogenesis of cardiac hypertrophy, although its mechanism of action remains largely unknown. To determine the effect of PPARα activation on endothelin-1 (ET-1)-induced cardiomyocyte hypertrophy and explore its molecular mechanisms, w...

journal_title：Archives of biochemistry and biophysics

authors： Le K,Li R,Xu S,Wu X,Huang H,Bao Y,Cai Y,Lan T,Moss J,Li C,Zou J,Shen X,Liu P

更新日期：2012-02-01 00:00:00

abstract：:Human NTPDase2 and chicken NTPDase8 are cell surface nucleotidases that contain two transmembrane domains (TMD) and five apyrase conserved regions (ACRs). ACR1 is located near the N-terminal TMD whereas ACR5 is located near the C-terminal TMD. The human NTPDase2 activity is decreased by low concentration of NP-40 and ...

journal_title：Archives of biochemistry and biophysics

authors： Chiang WC,Knowles AF

更新日期：2008-04-15 00:00:00

abstract：:Myosin prepared from tilapia (Serotherodon aureus) was complexed with 8-anilino-1-naphthalene sulfonate (ANS) and continuously heated at 1 degree C/min. A large increase in fluorescence was observed with a transition temperature of 34 degrees C. The effect of several salts on the transition temperature was tested. A p...

journal_title：Archives of biochemistry and biophysics

authors： Wicker L,Knopp JA

更新日期：1988-11-01 00:00:00

abstract：:The effects of polyunsaturated fatty acids and lipid peroxidation on LM fibroblast plasma membrane individual leaflet sterol distribution and structural order were examined. The cytofacial (inner) leaflet was more rigid and contained more sterol than the exofacial (outer) leaflet. The static (limiting anisotropy) and ...

journal_title：Archives of biochemistry and biophysics

authors： Schroeder F,Kier AB,Sweet WD

更新日期：1990-01-01 00:00:00

abstract：:Quantitative estimation of cytosolic Ca2+- and phospholipid-dependent protein kinase (PKC) activity was performed by polyacrylamide gel electrophoresis under nondenaturating conditions (PAGE). With this method less than 50 micrograms of cytosol protein can be accurately quantitated for PKC activity. The amount of cyto...

journal_title：Archives of biochemistry and biophysics

authors： Fabbro D,Jungmann RA,Eppenberger U

更新日期：1985-05-15 00:00:00

abstract：:The polymerization of alpha1-antitrypsin within the hepatic cell leads to alpha1-antitrypsin deficiency. Both the conformational changes and the kinetics of the polymerization process are poorly understood. Here we describe fluorescence experiments investigating the polymerization reaction using the fluorescent probe4...

journal_title：Archives of biochemistry and biophysics

authors： James EL,Bottomley SP

更新日期：1998-08-15 00:00:00

abstract：:Purification of the scrapie agent by methods using digestion with proteinase K yields a protein product, PrP-27-30, with an apparent mass of 27-30 kDa (D. C. Bolton et al. (1982) Science 218, 1309-1311; S. B. Prusiner et al. (1982) Biochemistry 21, 6942-6950). In contrast, a 33-37 kDa glycoprotein, HaSp33-37, was the ...

journal_title：Archives of biochemistry and biophysics

authors： Bolton DC,Bendheim PE,Marmorstein AD,Potempska A

更新日期：1987-11-01 00:00:00

abstract：:There is dose-dependent Cd(2+)-evoked swelling of isolated rat liver mitochondria energized by complex I, II, or IV respiratory substrates in sucrose medium in the absence of added Ca(2+) and P(i), which is prevented by Sr(2+). Permeability transition effectors (ADP, CsA, EGTA, RR, DTT, ATR, P(i), and Ca(2+)) affect i...

journal_title：Archives of biochemistry and biophysics

authors： Belyaeva EA,Glazunov VV,Korotkov SM

更新日期：2002-09-15 00:00:00

abstract：:An intermediate state of lentil lectin was characterized at pH 1 having low content of secondary as well as tertiary structure. Far- and near-UV CD spectroscopy showed loss of structure when pH was lowered from 7 to 0.8 but the structure loss was less than that of the protein in presence of 6M GndHCl. Intrinsic trypto...

journal_title：Archives of biochemistry and biophysics

authors： Naseem F,Khan RH

更新日期：2004-11-15 00:00:00

abstract：:Linamarase (EC 3.2.1.21) was purified from cassava petiole, stem, and root cortex by ammonium sulfate precipitation, column chromatography on Sepharose 6B, and chromatofocusing. The last step resolved the enzyme from each source into three forms with pI values of 4.3, 3.3, and 2.9. Each form was found to be oligomeric...

journal_title：Archives of biochemistry and biophysics

authors： Eksittikul T,Chulavatnatol M

更新日期：1988-10-01 00:00:00

abstract：:A novel action of the gonadotropic hormones of the adenohypophysis on the regulation of kidney heme metabolism and cytochrome P-450 concentrations is described. The treatment of rats with cis-platinum for 7 days caused a greater than twofold increase in the microsomal cytochrome P-450 and heme concentrations in the ki...

journal_title：Archives of biochemistry and biophysics

authors： Jollie DR,Maines MD

更新日期：1985-07-01 00:00:00

abstract：:Application of nanosecond pulsed electric fields (nsPEFs) has attracted attention as a unique tool in life sciences, especially for cancer therapy, but the molecular mechanism of its action on living organisms is yet to be fully elucidated. Here, we report a transient activation of signaling pathways involving mitogen...

journal_title：Archives of biochemistry and biophysics

authors： Morotomi-Yano K,Akiyama H,Yano K

更新日期：2011-11-01 00:00:00

abstract：:Adenylate cyclase activation by GTP and octopamine as well as basal activity (in the presence of Mg2+) have been studied as a function of membrane structure in plasma membranes from brain of the dipterous Ceratitis capitata. Benzyl alcohol and lidocaine, but not phenobarbital, inhibited the three activities to the sam...

journal_title：Archives of biochemistry and biophysics

authors： Guillen A,Haro A,Gavilanes FG,Municio AM

更新日期：1991-05-01 00:00:00

abstract：:Sodium pump was the first ion pump discovered. A member of the family of active transporters that catalyze adenosine 5'-triphosphate hydrolysis by forming a phosphorylated enzyme intermediate, sodium pump couples the energy released to unequal countertransport of sodium and potassium ions. The ion gradient generated b...

journal_title：Archives of biochemistry and biophysics

authors： Faller LD

更新日期：2008-08-01 00:00:00

abstract：:Cancer osaka thyroid (COT), a human MAP 3 K, is essential for lipopolysaccharide activation of the Erk MAPK cascade in macrophages. COT 30--467 is insoluble, whereas low levels of COT 30--397 can be expressed, but this protein is unstable. However, both COT 30--467 and COT 30--397 are expressed in a soluble and stable...

journal_title：Archives of biochemistry and biophysics

authors： Jia Y,Quinn CM,Bump NJ,Clark KM,Clabbers A,Hardman J,Gagnon A,Kamens J,Tomlinson MJ,Wishart N,Allen H

更新日期：2005-09-01 00:00:00