Abstract：
:Preparative isoelectric focusing and gel filtration chromatography were used to purify a carboxypeptidase produced by the entomopathogenic fungus Metarhizium anisopliae during growth on cockroach cuticle. The enzyme was inhibited by diisopropyl fluorophosphate, implying involvement of a serine residue in catalysis. However, the M. anisopliae enzyme differed from most serine carboxypeptidases in also being inhibited by the metal chelator 1,10-phenanthroline and in being a small (30 kDa), basic (pI 9.97) protein with a neutral pH optima (pH 6.8). These properties resemble those exhibited by some metalloproteases but the enzyme is not inhibited by Cd2+; nor do Zn2+ or Co2+ restore activity in enzyme inhibited with phenanthroline. The amino-terminal sequence (22 residues) showed no similarity to other protein sequences. Unlike previously reported fungal carboxypeptidases, the M. anisopliae enzyme is powerfully inhibited by potato carboxypeptidase inhibitor. The carboxypeptidase shows a broad primary specificity toward amino acids with hydrophobic side groups in a series of N-blocked dipeptides, with substrates with phenylalanine being the most rapidly hydrolyzed. The S1 subsite also accommodated Glu, confirming its low selectivity. Proline at P1 or P1 resulted in a very poor substrate. The specificity of the carboxypeptidase complements that of the subtilisin-like protease (Pr1) of M. anisopliae. Both Pr1 and the carboxypeptidase are produced during carbon and nitrogen deprivation, which indicates that the exopeptidase functions with Pr1 to degrade peptides to supply amino acids during starvation and pathogenicity.
journal_name
Arch Biochem Biophysjournal_title
Archives of biochemistry and biophysicsauthors
St Leger RJ,Bidochka MJ,Roberts DWdoi
10.1006/abbi.1994.1458subject
Has Abstractpub_date
1994-11-01 00:00:00pages
392-8issue
2eissn
0003-9861issn
1096-0384pii
S0003986184714585journal_volume
314pub_type
杂志文章abstract：:The application of molecular dynamics simulations to study the folding and dynamics of peptides has attracted a lot of interest in the last couple of decades. Following the successful prediction of the folding of several proteins using molecular simulation, foldable peptides emerged as a favourable system mainly due t...
journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章,评审
doi：10.1016/j.abb.2019.01.033
更新日期：2019-03-30 00:00:00
abstract：:CYP2D15 is the canine ortholog of human CYP2D6, the human CYP2D isoform involved in the metabolism of drugs such as antiarhythmics, adrenoceptor antagonists, and tricyclic antidepressants. Similar to human, canine CYP2D15 is expressed in the liver, with detectable levels in several other tissues. Three different CYP2D...
journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
doi：10.1006/abbi.1998.0801
更新日期：1998-09-01 00:00:00
abstract：:Inactivations of chicken liver pyruvate carboxylase with N-(7-dimethylamino-4-methyl-3-coumarinyl)maleimide (DACM) and o-phthalaldehyde (o-PA) have identified cysteine and lysine residues that are essential for catalytic activity. Protection experiments suggest that the modified residues are located in or near the fir...
journal_title：Archives of biochemistry and biophysics
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doi：10.1006/abbi.1993.1275
更新日期：1993-06-01 00:00:00
abstract：:A cDNA clone encoding troponin C of Akazara scallop (Chlamys nipponensis akazara) striated adductor muscle was isolated and sequenced. The cDNA is composed of 1987 bp and has an open reading frame of 462 bp at nucleotide positions 12-473. The amino acid sequence deduced from the cDNA showed 89% homology with that of E...
journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
doi：10.1006/abbi.1994.1237
更新日期：1994-06-01 00:00:00
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journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
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更新日期：1985-04-01 00:00:00
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journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
doi：10.1016/0003-9861(91)90323-b
更新日期：1991-02-15 00:00:00
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journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
doi：10.1016/0003-9861(89)90599-7
更新日期：1989-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：1989-07-01 00:00:00
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journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
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更新日期：1984-10-01 00:00:00
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更新日期：2016-09-01 00:00:00
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pub_type： 杂志文章
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更新日期：1985-01-01 00:00:00
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journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
doi：10.1016/0003-9861(89)90500-6
更新日期：1989-09-01 00:00:00
abstract：:Environmental pollution is a major cause of global mortality and burden of disease. All chemical pollution forms together may be responsible for up to 12 million annual excess deaths as estimated by the Lancet Commission on pollution and health as well as the World Health Organization. Ambient air pollution by particu...
journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章,评审
doi：10.1016/j.abb.2020.108662
更新日期：2020-12-15 00:00:00
abstract：:A number of natural or synthetic compounds as AGE inhibitors have been proposed, discovered or currently being advanced by others and us. We have identified two new classes of aromatic compounds; aryl- (and heterocyclic) ureido and aryl (and heterocyclic) carboxamido phenoxyisobutyric acids, and benzoic acid derivativ...
journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章,评审
doi：10.1016/j.abb.2003.08.009
更新日期：2003-11-01 00:00:00
abstract：:The nucleotide sequence of tyrosine tRNAQ* psi A from bovine liver was determined to be pC-C-U-U-C-m2G-A-U-A-m2G-C-U-C-A-G-D-D-G-G-acp3U-A-G-A -G-C-m22G-m22G -A-G-G-A-C-U-Q*-psi-A-m1G-A-psi m-C-C-U-U-A-G-m7G-D-m5C-G-C-U-G-G-T-psi-C-G-m1A -U-U-C-C-G-G-C-U-C-G-A-A-G-G-A-C-C-AOH. This tyrosine tRNA is 76 nucleotides in l...
journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
doi：10.1016/0003-9861(85)90647-2
更新日期：1985-01-01 00:00:00
abstract：:NahR, a member of the LysR regulator family, is a positive transcriptional regulator for genes of the naphthalene degradation pathway in Pseudomonas sp. To study NahR binding properties, five single and six double mutants were made at residues 169 and/or 248, which are located in the central inducer recognition domain...
journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
doi：10.1016/j.abb.2004.10.020
更新日期：2005-02-01 00:00:00
abstract：:Dethiolation of proteins (reduction of protein mixed disulfides) by NADPH-dependent and glutathione (GSH)-dependent enzymes, and by nonenzymatic reaction with GSH, was studied by electrofocusing methodology with glycogen phosphorylase b and creatine kinase as substrates. Phosphorylase b was not rapidly dethiolated by ...
journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
doi：10.1016/0003-9861(89)90413-x
更新日期：1989-10-01 00:00:00
abstract：:To assess the indirect effects of snake venom metalloproteinases (SVMP) on host tissue local necrosis, we investigated the effect of the SVMP jararhagin on the gene expression profiles of human fibroblasts in vitro and mouse tissue in vivo. Two functional classes of up-regulated proteins, cell death and inflammatory d...
journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
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更新日期：2005-09-01 00:00:00
abstract：:Rat hepatic 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase was purified to homogeneity using agarose-HMG-CoA affinity chromatography. Additional protein was isolated from the affinity column with 0.5 M KCl that demonstrated no HMG-CoA reductase activity, yet comigrated with purified HMG-CoA reductase on sod...
journal_title：Archives of biochemistry and biophysics
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doi：10.1016/0003-9861(84)90428-4
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doi：10.1016/j.abb.2004.12.019
更新日期：2005-03-15 00:00:00
abstract：:Vitamin D was discovered as an anti-rachitic agent, but even at present, there is no direct evidence to support the concept that vitamin D directly stimulates osteoblastic bone formation and mineralization. It appears to be paradoxical, but vitamin D functions in the process of osteoclastic bone resorption. In 1952, C...
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pub_type： 杂志文章,评审
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更新日期：2012-07-01 00:00:00
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journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
doi：10.1016/0003-9861(89)90237-3
更新日期：1989-08-01 00:00:00
abstract：:In order to specify that protein labeling is the result of mono-ADP ribosylation, a careful evaluation of the reaction conditions and products is necessary. To investigate the specificity and target proteins of the arginine-specific mono-ADP-ribosyltransferase (mADP-RT) in rabbit skeletal muscle T-tubules (TT) biotin-...
journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
doi：10.1006/abbi.1997.0330
更新日期：1997-11-15 00:00:00
abstract：:The hydrolysis of 32 X-phenyl-N-methanesulfonyl glycinates by papain was investigated. It was found that the variation in the Michaelis constants could be rationalized by the following correlation equation: log 1/Km = 0.61 pi '3 + 0.46 MR4 + 0.55 sigma + 2.00 with a correlation coefficient of 0.945. In this expression...
journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
doi：10.1016/0003-9861(84)90136-x
更新日期：1984-02-15 00:00:00
abstract：:Calcium-induced changes in protein solubility play a role in a variety of important biological processes including the deposition of bone and dentin and the secretion of milk. The phenomena of salt-induced (calcium) precipitation of proteins (salting-out), and the resolubilization of these proteins at higher salt conc...
journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
doi：10.1016/0003-9861(88)90380-3
更新日期：1988-08-15 00:00:00
abstract：:The mechanism by which diverse drugs modulate voltage-dependent Ca2+ channels is ill-understood. We have approached this problem by examining the interaction of verapamil with a 97-residue synthetic channel peptide (SCP) that exhibits functional similarities to authentic L-type Ca2+ channels in terms of cation selecti...
journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
doi：10.1006/abbi.1997.9968
更新日期：1997-05-15 00:00:00
abstract：:We evaluated the expression and activity of rat 12-lipoxygenase (LO) in rat intestinal epithelial (RIE) cells during apoptosis and cell differentiation. Sodium butyrate (NaBT) treatment induced wild-type RIE (W-RIE) cells to undergo differentiation and apoptosis. Alkaline phosphatase (ALP) activity, a marker of cell d...
journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
doi：10.1006/abbi.1999.1284
更新日期：1999-08-01 00:00:00
abstract：:In 5-aminolevulinic acid (ALA)-based photodynamic therapy (PDT), ALA taken up by tumor cells is metabolized to protoporphyrin IX (PpIX), which sensitizes photodamage leading to apoptotic or necrotic cell death. Since lipophilic PpIX originates in mitochondria, we postulated that photoperoxidation of highly unsaturated...
journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
doi：10.1016/j.abb.2004.09.025
更新日期：2005-01-15 00:00:00
abstract：:Phospholipid transmethylation in the microsomal fraction of stimulated and unstimulated human leukocytes was measured in a recently developed assay system. Microsomal fraction was prepared from neutrophils, unseparated lymphocytes, T lymphocytes, and non-T lymphocytes by sonication and subsequent ultracentrifugation. ...
journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
doi：10.1016/0003-9861(84)90318-7
更新日期：1984-10-01 00:00:00
abstract：:Staphylococcus aureus is associated with several clinically significant infections among humans and infections associated with antibiotic-resistant strains are growing in frequency. Antivirulence strategies shift the target of drugs from bacterial growth to the infection process resulting to milder evolutionary pressu...
journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
doi：10.1016/j.abb.2018.03.010
更新日期：2018-05-01 00:00:00
