The binding of 8-anilino-1-naphthalene sulfonate (ANS) to fish myosin and the effect of salts on the thermal transitions of fish myosin-ANS complex.

abstract：:A quantitative assay has been developed to measure holocarboxylase synthetase activity in cellular extracts. This assay was based on measuring the incorporation of [3H]biotin of high specific activity (4.3 Ci/mmol) into purified rat liver apopyruvate carboxylase. With this assay, holocarboxylase synthetase in 3T3-L1 m...

journal_title：Archives of biochemistry and biophysics

authors： Chang HI,Cohen ND

更新日期：1983-08-01 00:00:00

abstract：:The binding of Toxin A isolated from Clostridium difficile to rabbit erythrocyte glycolipids has been studied. Total lipid extracts from rabbit erythrocytes were subjected to thin-layer chromatography and toxin-binding glycolipids detected by using 125I-labeled Toxin A in a direct binding overlay technique. Two major ...

journal_title：Archives of biochemistry and biophysics

authors： Clark GF,Krivan HC,Wilkins TD,Smith DF

更新日期：1987-08-15 00:00:00

abstract：:Low-molecular-weight (LMW) thiols are a class of highly reactive compounds massively involved in the maintenance of cellular redox homeostasis. They are implicated in plant responses to almost all stress factors, as well as in the regulation of cellular metabolism. The most studied LMW thiols are glutathione and its b...

journal_title：Archives of biochemistry and biophysics

authors： Pivato M,Fabrega-Prats M,Masi A

更新日期：2014-10-15 00:00:00

abstract：:The objective of this study was to determine if oxidative stress/damage is a possible causal factor in the senescence-related loss of brain functions in the mouse. If such a relationship indeed existed, it was expected that oxidative protein damage would increase with age within regions of the brain associated with se...

journal_title：Archives of biochemistry and biophysics

authors： Dubey A,Forster MJ,Lal H,Sohal RS

更新日期：1996-09-01 00:00:00

abstract：:The effects of glycosylation on the stability and subunit interactions of vicilin, the major storage protein in pea seeds, were investigated. Glycosylated vicilin derivatives were prepared by alkylation of lysine epsilon-amino groups with various carbohydrates. Average modification levels of 13.4 +/- 3.0, 11.1 +/- 3.6...

journal_title：Archives of biochemistry and biophysics

authors： Pedrosa C,De Felice FG,Trisciuzzi C,Ferreira ST

更新日期：2000-10-15 00:00:00

abstract：:Several previously unreported small heat-shock proteins (sHsps) were detected in mitochondria from heat-stressed rat PC12 cells, but not in unstressed controls. Functional inactivation of the mitochondrial sHsps with murine Hsp25 antibody indicated that these sHsps protect NADH:ubiquinone oxidoreductase and NADH dehyd...

journal_title：Archives of biochemistry and biophysics

authors： Downs CA,Jones LR,Heckathorn SA

更新日期：1999-05-15 00:00:00

abstract：:The so-called 90,000-Da heat shock protein (hsp90) from chicken liver has been purified and physically characterized in the presence of high levels of the serine phosphatase inhibitor fluoride. The protein is an elongated dimer with a molecular weight of 160,000 and a frictional ratio of 1.6. On two-dimensional electr...

journal_title：Archives of biochemistry and biophysics

authors： Iannotti AM,Rabideau DA,Dougherty JJ

更新日期：1988-07-01 00:00:00

abstract：:The regulation of the plasma membrane Ca2+ pump by hormones via phosphorylation in intact cells has not been clearly established. We now present evidence that the Ca2+ pump is phosphorylated on both serine and threonine residues in unstimulated and stimulated cultured rat aortic endothelial cells. Among the stimuli te...

journal_title：Archives of biochemistry and biophysics

authors： Wang KK,Du YS,Diglio C,Tsang W,Kuo TH

更新日期：1991-08-15 00:00:00

abstract：:The keto form of oxaloacetate (OAA), a product of phosphoenolpyruvate carboxylase (PEPC) activity, can undergo various nonenzymatic conversions which make conventional methods of assaying the enzyme difficult, because the products may either act as inhibitors or go undetected. In studies with PEPC isolated from leaves...

journal_title：Archives of biochemistry and biophysics

authors： Walker GH,Ku MS,Edwards GE

更新日期：1986-08-01 00:00:00

abstract：:Phenylalanine ammonia-lyase forms trans-cinnamate from L-phenylalanine, and thus stands at a gateway to secondary metabolism in higher plants. L-alpha-Amino-oxy-beta-phenylpropanoic acid (L-AOPP), a very effective competitive inhibitor of this enzyme, is most probably a transition-state analog for the elimination reac...

journal_title：Archives of biochemistry and biophysics

authors： Jones DH,Northcote DH

更新日期：1984-11-15 00:00:00

abstract：:We have shown that superoxide (O2.-) is produced during the oxidation of veratryl alcohol by lignin peroxidase (LiP) by the reaction of the veratryl alcohol cation radical with hydrogen peroxide (D. B. Barr, M. M. Shah, and S. D. Aust, 1993, J. Biol. Chem. 268, 241-244). Compound III, an inactive form of peroxidases c...

journal_title：Archives of biochemistry and biophysics

authors： Barr DP,Aust SD

更新日期：1994-06-01 00:00:00

abstract：:Sucrose-phosphate synthase (SPS; EC 2.4.1.14) is regulated in part by reversible protein phosphorylation. When dephospho-SPS is partially purified from illuminated spinach leaves and incubated with [gamma-32P]ATP the enzyme is phosphorylated by a copurifying protein kinase. In this report, 32P-phosphopeptides from try...

journal_title：Archives of biochemistry and biophysics

authors： McMichael RW Jr,Klein RR,Salvucci ME,Huber SC

更新日期：1993-12-01 00:00:00

abstract：:A58 is a murine monoclonal antibody raised against the internal dimeric subunit A alpha 1 of the hemocyanin of the scorpion Androctonus australis. When A58 is tested against a range of related hemocyanins, it exhibits cross-reactivity with a limited number of scorpion species. All of them belong to a single Scorpion F...

journal_title：Archives of biochemistry and biophysics

authors： Billiald P,Motta G,Vaux DJ

更新日期：1995-03-10 00:00:00

abstract：:Lipoxygenase-induced lipid oxidation contributes to plasma lipoprotein oxidation and may be an underlying pathogenic mechanism of atherogenesis. Since inactivation of the vasorelaxant actions of nitric oxide (.NO) plays a critical role in the impaired function of atherosclerotic vessels and because .NO reacts rapidly ...

journal_title：Archives of biochemistry and biophysics

authors： Rubbo H,Parthasarathy S,Barnes S,Kirk M,Kalyanaraman B,Freeman BA

更新日期：1995-12-01 00:00:00

abstract：:Periodate-oxidized NADP+ (dialdehyde-NADP+) inactivated soluble ferredoxin-NADP+ oxidoreductase and combined covalently to the enzyme. This inactivation was first order with respect to dialdehyde-NADP+ and followed saturation kinetics, indicating that the enzyme initially forms a reversible complex with the inactivato...

journal_title：Archives of biochemistry and biophysics

authors： Chan RL,Carrillo N

更新日期：1984-02-15 00:00:00

abstract：:We have previously identified and characterized GSHPx-GI, which is a cellular selenium-dependent glutathione peroxidase (GSHPx) distinct from the classic GSHPx-1 and phospholipid hydroperoxide glutathione peroxidase (PHGPX). We have determined the level of GSHPx-GI mRNA expression in the rat gastrointestinal tract fro...

journal_title：Archives of biochemistry and biophysics

authors： Chu FF,Esworthy RS

更新日期：1995-11-10 00:00:00

abstract：:We have previously shown that rat hepatic cytochromes P450f, P450g, P450h, and P450i possess a high degree of immunochemical and, presumably, structural relatedness. Polyclonal antibodies directed against cytochromes P450f and P450g were made monospecific by immunoabsorption against the cross-reactive proteins. The sp...

journal_title：Archives of biochemistry and biophysics

authors： Bandiera S,Ryan DE,Levin W,Thomas PE

更新日期：1986-08-01 00:00:00

abstract：:DNA damage has been postulated as a mechanism of neuronal death in Parkinson's disease (PD). In the present study, genomic DNA was isolated from eight brain regions (frontal, temporal, and occipital cortex, hippocampus, caudate/putamen, thalamus, cerebellum, and midbrain) from five neuropathologically confirmed cases ...

journal_title：Archives of biochemistry and biophysics

authors： Hegde ML,Gupta VB,Anitha M,Harikrishna T,Shankar SK,Muthane U,Subba Rao K,Jagannatha Rao KS

更新日期：2006-05-15 00:00:00

abstract：:Dopamine beta-hydroxylase present in the soluble matrix of bovine adrenal medullary chromaffin granules contains biantennary complex oligosaccharides and high-mannose oligosaccharides in a molar ratio of approximately 2:1. The high-mannose oligosaccharides contain an average of six mannose residues. The largest biante...

journal_title：Archives of biochemistry and biophysics

authors： Margolis RK,Finne J,Krusius T,Margolis RU

更新日期：1984-02-01 00:00:00

abstract：:The sensitivity of animals to endotoxin differs significantly between species. Thus, factors that determine the susceptibility to endotoxin may play important roles in the pathogenesis of septic shock. In order to determine the mechanism responsible for susceptibility to endotoxin, the effect of lipopolysaccharide (LP...

journal_title：Archives of biochemistry and biophysics

authors： Kida T,Kuroki T,Kobayashi K,Inoue M

更新日期：1994-07-01 00:00:00

abstract：:A type II ribosome-inactivating protein (RIP) was isolated from the bark tissue of Japanese elderberry (Sambucus sieboldiana) and named sieboldin-b. Sieboldin-b is a heterodimeric protein consisting of 27- and 33-kDa subunits and showed strong ribosome-inactivating activity in vitro but did not show in vivo toxicity. ...

journal_title：Archives of biochemistry and biophysics

authors： Rojo MA,Yato M,Ishii-Minami N,Minami E,Kaku H,Citores L,Girbés T,Shibuya N

更新日期：1997-04-15 00:00:00

abstract：:We have previously demonstrated that gangliosides were able to modulate the plasma membrane Ca2+-ATPase (PMCA) from porcine brain synaptosomes and porcine erythrocytes [Y. Zhao, X. Fan, F. Yang, X. Zhang, Arch. Biochem. Biophys. 427 (2004) 204-212 and J. Zhang, Y. Zhao, J. Duan, F. Yang, X. Zhang, Arch. Biochem. Bioph...

journal_title：Archives of biochemistry and biophysics

authors： Duan J,Zhang J,Zhao Y,Yang F,Zhang X

更新日期：2006-10-15 00:00:00

abstract：:We examined effects of three structurally related pyridinium compounds, 1-methyl-4-phenylpyridinium (MPP+), paraquat, and 1-methyl-4-(4'-nitrophenyl) pyridinium (analog 1), on the energy metabolism in pheochromocytoma PC12 cells. MPP+ inhibited the intracellular NADH oxidation by the mitochondrial respiratory chain, j...

journal_title：Archives of biochemistry and biophysics

authors： Kang D,Miyako K,Kuribayashi F,Hasegawa E,Mitsumoto A,Nagano T,Takeshige K

更新日期：1997-01-01 00:00:00

abstract：:From mistletoe Viscum album L. extracts three chitin-binding lectin isoforms, cbML1, cbML2, and cbML3, were isolated and their primary structure determined. All three cbML isoforms are composed of two protein chains of 48 or 49 amino acid residues, linked by an intermolecular disulfide bond. The sequence of each singl...

journal_title：Archives of biochemistry and biophysics

authors： Stoeva S,Franz M,Wacker R,Krauspenhaar R,Guthöhrlein E,Mikhailov A,Betzel C,Voelter W

更新日期：2001-08-01 00:00:00

abstract：:Estrogen-induced chick oviduct differentiation is accompanied by an increased capacity for protein glycosylation. A portion of this increase has been attributed to increased levels of dolichyl phosphate (Dol-P). Hormone withdrawal leads to an apparent decrease in Dol-P. Dol-P metabolism in the oviduct has been studied...

journal_title：Archives of biochemistry and biophysics

authors： DeRosa PA,Lucas JJ

更新日期：1984-11-01 00:00:00

abstract：:The subcellular mechanism of alkenyl halide S-conjugate-induced nephrotoxicity was studied in mitochondria isolated from rat kidney cortex in vitro using the cysteine conjugate of hexachloro-1,3-butadiene, i.e., S-pentachlorobutadienyl-L-cysteine (PCBC) as a model substrate. Respiring mitochondria exposed to various c...

journal_title：Archives of biochemistry and biophysics

authors： Wallin A,Jones TW,Vercesi AE,Cotgreave I,Ormstad K,Orrenius S

更新日期：1987-11-01 00:00:00

abstract：:Xanthophyllomces dendrorhous (in asexual state named as Phaffia rhodozyma) is a fungus which produces astaxanthin, a high value carotenoid used in aquafarming. Genetic pathway engineering is one of several steps to increase the astaxanthin yield. The limiting enzyme of the carotenoid pathway is phytoene synthase. Inte...

journal_title：Archives of biochemistry and biophysics

authors： Ledetzky N,Osawa A,Iki K,Pollmann H,Gassel S,Breitenbach J,Shindo K,Sandmann G

更新日期：2014-03-01 00:00:00

abstract：:gG.2 glycoprotein was purified by H966 monoclonal antibodies linked to Sepharose from herpes simplex virus type 2-infected HEp-2 cells labeled with [3H] glucosamine. The glycoprotein was subjected to Pronase digestion and the glycopeptides were fractionated by Con A-Sepharose in a major fraction (88.5% of total radioa...

journal_title：Archives of biochemistry and biophysics

authors： Serafini-Cessi F,Malagolini N,Dall'Olio F,Pereira L,Campadelli-Fiume G

更新日期：1985-08-01 00:00:00

abstract：:ATP-activated P2Y receptors play an important role in renal sodium excretion. The aim of this study was to evaluate the modulation of ATPase-driven sodium reabsorption in the proximal tubule by ATP or adenosine (Ado). LLC-PK1 cells, a model of porcine proximal tubule cells, were used. ATP (10(-6)M) or Ado (10(-6)M) sp...

journal_title：Archives of biochemistry and biophysics

authors： Wengert M,Ribeiro MC,Abreu TP,Coutinho-Silva R,Leão-Ferreira LR,Pinheiro AA,Caruso-Neves C

更新日期：2013-07-15 00:00:00

abstract：:The putrescine analogue 1,4-diamino-2-butanone (DAB) is highly toxic to various microorganisms, including Trypanosoma cruzi. Similar to other α-aminocarbonyl metabolites, DAB exhibits pro-oxidant properties. DAB undergoes metal-catalyzed oxidation yielding H(2)O(2), NH(4)(+) ion, and a highly toxic α-oxoaldehyde. In v...

journal_title：Archives of biochemistry and biophysics

authors： Soares CO,Colli W,Bechara EJ,Alves MJ

更新日期：2012-12-15 00:00:00