Changes of energy metabolism induced by 1-methyl-4-phenylpyridinium (MPP+)-related compounds in rat pheochromocytoma PC12 cells.

abstract：:Several previously unreported small heat-shock proteins (sHsps) were detected in mitochondria from heat-stressed rat PC12 cells, but not in unstressed controls. Functional inactivation of the mitochondrial sHsps with murine Hsp25 antibody indicated that these sHsps protect NADH:ubiquinone oxidoreductase and NADH dehyd...

journal_title：Archives of biochemistry and biophysics

authors： Downs CA,Jones LR,Heckathorn SA

更新日期：1999-05-15 00:00:00

abstract：:Proteolysis of glucose dehydrogenase from Bacillus megaterium with proteinase K apparently generated two fragments. The small fragment, designated as K-peptide, was sequenced and its covalent structure was determined as Ser-Ser-Glu-Ala-Ser-Tyr-Val-Thr-Gly-Ile-Thr-Leu-Phe-Ala-Asp-Gly-Gly-Met-Thr- Gln-Tyr-Pro-Ser-Phe-Gl...

journal_title：Archives of biochemistry and biophysics

authors： Jany KD,Nitsche E

更新日期：1984-02-15 00:00:00

abstract：:The most common form of muscular dystrophy is known as Myotonic dystrophy Type 1 (DM1) in adults. It was aimed to investigate the relationship between antioxidant imbalance and diaphragm thickness with pulmonary function test results in peripheral blood of Myotonic Dystrophy Type 1 patients. In the prospective study, ...

journal_title：Archives of biochemistry and biophysics

authors： Koc F,Atli G,Menziletoglu SY,Kose S

更新日期：2020-02-15 00:00:00

abstract：:The NADH-dependent microsomal electron transfer system consists of NADH-cytochrome b5 reductase and cytochrome b5, which donates reducing equivalents to fatty acyl desaturase, cytochrome P450, and other reactions. A study was carried out to investigate the interaction of NADH with several ferric complexes and to evalu...

journal_title：Archives of biochemistry and biophysics

authors： Yang MX,Cederbaum AI

更新日期：1995-12-20 00:00:00

abstract：:A fraction containing a protein-glycogen complex was isolated from rat skeletal muscle in order to study the effect of hypothyroidism on phosphorylase activation in this structural and functional unit of the glycogenolytic process. The total activities of phosphorylase and phosphorylase phosphatase in euthyroids and h...

journal_title：Archives of biochemistry and biophysics

authors： Leijendekker WJ,Edauw P,van Hardeveld C,Simonides WS

更新日期：1989-10-01 00:00:00

abstract：:The adrenal gland synthesizes steroid hormones from the adrenal cortex and catecholamines from the adrenal medulla. Both cortisol and adrenal androgens can have powerful effects on bone. The overproduction of cortisol in Cushing's disease leads to a dramatic reduction in bone density and an increase risk of fracture. ...

journal_title：Archives of biochemistry and biophysics

authors： Hardy R,Cooper MS

更新日期：2010-11-01 00:00:00

abstract：:The enzyme ketol acid reductoisomerase catalyzes the second common reaction in the biosynthesis of the branched chain amino acids. The reaction is complex as an alkyl migration and a ketone reduction apparently occur as separate steps during the conversion of acetolactate to 2,3-dihydroxy-3-methylbutyrate. This paper ...

journal_title：Archives of biochemistry and biophysics

authors： Mrachko GT,Chunduru SK,Calvo KC

更新日期：1992-05-01 00:00:00

abstract：:A new fluorescent ATP analog, adenosine-5'-triphosphoro-gamma-1-(5-sulfonic acid)naphthyl ethylamidate (gamma-1,5-EDANS)ATP, containing the fluorophore N-(aminoethyl)-5-naphthylamine-1-sulfonic acid attached via a gamma-phosphoamidate bond was synthesized in good yield. It has absorption maxima at 255 and 344 nm and a...

journal_title：Archives of biochemistry and biophysics

authors： Wu FY,Abdulwajid AW,Solaiman D

更新日期：1986-05-01 00:00:00

abstract：:According to differences in mobility on SDS-polyacrylamide gel electrophoresis, calpastatins (inhibitor proteins of the calcium-dependent proteinase calpain) are classified into the tissue type (100-120 kDa) and the erythrocyte type (70 kDa), which lacks the amino-terminal domains (domains L and 1). We investigated th...

journal_title：Archives of biochemistry and biophysics

authors： Takano E,Nosaka T,Lee WJ,Nakamura K,Takahashi T,Funaki M,Okada H,Hatanaka M,Maki M

更新日期：1993-06-01 00:00:00

abstract：:The occurrence of a variety of purine receptors in the immune system indicates that extracellular purines play important functional roles. Extracellular purine concentrations are, in great part, determined by ectonucleotidases, namely, the ATP diphosphohydrolase, also identified as CD39, a lymphocyte cell surface mark...

journal_title：Archives of biochemistry and biophysics

authors： Benrezzak O,Grondin G,Sévigny J,Gendron FP,Rousseau E,D'Orléans-Juste P,Beaudoin AR

更新日期：1999-10-15 00:00:00

abstract：:The effects of nonlamellar-prone lipids, diacylglycerol and phosphatidylethanolamine (PE), on the kinetic association of SecA with model membranes were examined by measuring changes in the intrinsic emission fluorescence with a stopped-flow apparatus. Upon interaction with standard liposomes composed of 50 mol% dioleo...

journal_title：Archives of biochemistry and biophysics

authors： Ahn T,Kim JS,Lee BC,Yun CH

更新日期：2001-11-01 00:00:00

abstract：:The 3-dimensional structure determination of rat phenylalanine hydroxylase (PAH) has identified potentially important amino acids lining the active site cleft with the majority of these having hydrophobic side-chains including several with aromatic side chains. Here we have analyzed the effect on rat PAH enzyme kineti...

journal_title：Archives of biochemistry and biophysics

authors： Jennings IG,Cotton RG,Kobe B

更新日期：2000-12-15 00:00:00

abstract：:The effect of a protein matrix on the processing of glycoprotein glycans by Golgi enzymes from plant seedlings has been determined with an artificial glycoprotein system, comparing the processing rates of glycan-(biotinyl)Asn (or glycan-(biotinamidohexanoyl)Asn) substrates either free or bound to avidin. An analysis o...

journal_title：Archives of biochemistry and biophysics

authors： Lu Y,Wold F

更新日期：1991-04-01 00:00:00

abstract：:Differential light absorption spectra of the bilirubin-albumin 1:1 complex, obtained on addition of 20 different sulfonamides, differ with respect to shape and amplitude. This finding seems to indicate that the sulfonamide molecule is bound in direct touch with the bilirubin. The light absorption spectrum of bilirubin...

journal_title：Archives of biochemistry and biophysics

authors： Brodersen R,Knudsen A,Pedersen AO

更新日期：1987-02-01 00:00:00

abstract：:UDP-galactose 4-epimerase from Escherichia coli is a homodimer of molecular weight 39 kDa/subunit having noncovalently bound NAD acting as cofactor. Denaturation by 8 M urea at pH 7.0 causes 85% loss of its secondary structure and dissociation of its constituent molecules. Dilution of the denaturant by buffer at pH 8....

journal_title：Archives of biochemistry and biophysics

authors： Barat B,Bhattacharyya D

更新日期：2001-07-15 00:00:00

abstract：:The hydrolysis of 32 X-phenyl-N-methanesulfonyl glycinates by papain was investigated. It was found that the variation in the Michaelis constants could be rationalized by the following correlation equation: log 1/Km = 0.61 pi '3 + 0.46 MR4 + 0.55 sigma + 2.00 with a correlation coefficient of 0.945. In this expression...

journal_title：Archives of biochemistry and biophysics

authors： Carotti A,Smith RN,Wong S,Hansch C,Blaney JM,Langridge R

更新日期：1984-02-15 00:00:00

abstract：:The steady-state kinetic mechanism of vitamin K-dependent carboxylase from calf liver has been investigated by initial-velocity measurements with varying concentrations of two carboxylase substrates and constant, nonsaturating concentrations of the other two substrates. With all combinations of the varied substrates t...

journal_title：Archives of biochemistry and biophysics

authors： Uotila L

更新日期：1988-07-01 00:00:00

abstract：:Complete structures are described for three urinary oligodextrins from one patient with type II and one patient with type III glycogen storage disease. GLC-MS, direct probe MS, and 1H NMR demonstrate two heptasaccharides and one hexasaccharide containing only alpha 1-4 and alpha 1-6 linkages. The observation that all ...

journal_title：Archives of biochemistry and biophysics

authors： Kumlien J,Grönberg G,Nilsson B,Månsson O,Zopf D,Lundblad A

更新日期：1989-03-01 00:00:00

abstract：:Uridine phosphorylase is one of the critical enzymes in the pyrimidine salvage pathway. Cells regenerate uridine for nucleotide metabolism by incorporating uracil with ribose-1-phosphate with this enzyme. Recent studies indicate that Escherichia coli uridine phosphorylase is destabilized in the presence of ATP. Howeve...

journal_title：Archives of biochemistry and biophysics

authors： Liu YK,Lin TH,Liu PF

更新日期：2017-11-15 00:00:00

abstract：:Anthocyanins are the main group of natural hydrosoluble pigments in plants. They introduce colouring to foods, with colours ranging from blue to red and orange. Nowadays, their importance for the Food and Pharmaceutical industries is mainly based in the existing scientific work evidencing their beneficial effects on t...

journal_title：Archives of biochemistry and biophysics

authors： de Pascual-Teresa S

更新日期：2014-10-01 00:00:00

abstract：:The sensitivity of animals to endotoxin differs significantly between species. Thus, factors that determine the susceptibility to endotoxin may play important roles in the pathogenesis of septic shock. In order to determine the mechanism responsible for susceptibility to endotoxin, the effect of lipopolysaccharide (LP...

journal_title：Archives of biochemistry and biophysics

authors： Kida T,Kuroki T,Kobayashi K,Inoue M

更新日期：1994-07-01 00:00:00

abstract：:We have previously shown that melittin, a bee venom peptide, potently inhibited the catalytic and transport functions of rabbit gastric (H+ + K+)ATPase. A radioactive photoaffinity analog of melittin, ([125I]azidosalicylyl melittin), labeled the (H+ + K+)ATPase. These results suggested that melittin exerted inhibitory...

journal_title：Archives of biochemistry and biophysics

authors： Cuppoletti J

更新日期：1990-05-01 00:00:00

abstract：:Biotin synthase catalyzes the insertion of a sulfur atom between two carbon atoms of dethiobiotin to form biotin in the last step of the biotin biosynthesis pathway. In Escherichia coli, biotin synthase is coded for by bioB gene. We report here cloning, sequencing, and initial functional characterization of the yeast ...

journal_title：Archives of biochemistry and biophysics

authors： Zhang S,Sanyal I,Bulboaca GH,Rich A,Flint DH

更新日期：1994-02-15 00:00:00

abstract：:Multidrug resistance (MDR) caused by overexpressed permeability-glycoprotein (P-gp) in cancer cells is the main barrier for the cure of cancers. P-gp can pump many chemotherapeutic drugs, which is a viable target to overcome P-gp-mediated MDR by efficient inhibitors of P-gp. However, limited understanding of the efflu...

journal_title：Archives of biochemistry and biophysics

authors： Wang L,Sun Y

更新日期：2020-12-15 00:00:00

abstract：:Recent X-ray crystal structures of human cytochrome P450 2B6 and rabbit cytochrome P450 2B4 in complex with amlodipine showed two bound ligand molecules, one in the active site and one in the substrate access channel. Based on the X-ray crystal structures, we investigated the interactions of P450 2B4 and 2B6 with amlo...

journal_title：Archives of biochemistry and biophysics

authors： Jang HH,Davydov DR,Lee GY,Yun CH,Halpert JR

更新日期：2014-03-01 00:00:00

abstract：:Systemic exposure to gram-negative bacterial substances such as lipopolysaccharide (LPS, or endotoxin) induces an uncontrolled, massive inflammatory reaction which culminates in multiple system organ failure and death. Septic shock often does not respond to corticosteroids; however, certain low-molecular-weight antiox...

journal_title：Archives of biochemistry and biophysics

authors： Stewart CA,Hyam K,Wallis G,Sang H,Robinson KA,Floyd RA,Kotake Y,Hensley K

更新日期：1999-05-01 00:00:00

abstract：:The common notion in the protein world holds that proteins are synthesized as a linear polypeptide chain, followed by folding into a unique, functional 3D-structure. As outlined in many articles of this volume, this is in fact the case for a great proportion of the proteome. Many proteins and protein domains, however,...

journal_title：Archives of biochemistry and biophysics

authors： Kovacs D,Szabo B,Pancsa R,Tompa P

更新日期：2013-03-01 00:00:00

abstract：:In cells expressing only the Glut1 isoform of glucose transporters, we have shown that glucose transport is markedly stimulated in response to hypoxia or inhibition of oxidative phosphorylation, conditions that would be expected to cause a stimulation of AMP-activated protein kinase (AMPK) activity. In the present stu...

journal_title：Archives of biochemistry and biophysics

authors： Abbud W,Habinowski S,Zhang JZ,Kendrew J,Elkairi FS,Kemp BE,Witters LA,Ismail-Beigi F

更新日期：2000-08-15 00:00:00

abstract：:B4-2-1 cells (Lec15 cells) are Chinese hamster ovary cells deficient in mannosylphosphoryldolichol synthase activity. They synthesize the truncated lipid intermediate Man5GlcNAc2-P-P-dolichol rather than the Glc3Man9GlcNAc2-P-P-dolichol synthesized by wild-type cells. In this report we present evidence that these cell...

journal_title：Archives of biochemistry and biophysics

authors： Stoll J,Cacan R,Verbert A,Krag SS

更新日期：1992-12-01 00:00:00

abstract：:We have studied the time-resolved fluorescence of three engineered Tet repressor (TetR) mutants bearing a single Trp residue at positions 162, 163, and 165 in the C-terminal part of the loop joining helices 8 and 9. Detailed analysis indicates that, at 20 degrees, the fluorescence decay of each Trp can be described as...

journal_title：Archives of biochemistry and biophysics

authors： Alberti P,Bombarda E,Kintrup M,Hillen W,Lami H,Piémont E,Doglia SM,Chabbert M

更新日期：1997-10-15 00:00:00