Stimulation of AMP-activated protein kinase (AMPK) is associated with enhancement of Glut1-mediated glucose transport.

abstract：:The nonenzymatic reaction of the cytotoxic compounds menadione (2-methyl-1,4-naphthoquinone) and 1,4-naphthoquinone (a reactive metabolite of 1-naphthol) with reducing agents such as NADPH and glutathione led to the formation of semiquinone-free radicals, which were detected with electron spin resonance spectroscopy. ...

journal_title：Archives of biochemistry and biophysics

authors： Takahashi N,Schreiber J,Fischer V,Mason RP

更新日期：1987-01-01 00:00:00

abstract：:The intracellular concentration of many steroids and xenobiotics is influenced by the membrane protein P-glycoprotein (Pgp). It has been inferred that the intracellular retention of many drugs that upregulate Pgp or modulate Pgp function might also be affected by Pgp. However, the ability of Pgp to influence the trans...

journal_title：Archives of biochemistry and biophysics

authors： Schuetz EG,Yasuda K,Arimori K,Schuetz JD

更新日期：1998-02-15 00:00:00

abstract：:Four subunits of beta-conglycinin were purified from soybean cultivar CX 635-1-1-1, and were designated alpha, alpha', beta, and beta' in accordance with nomenclature proposed by Thanh and Shibasaki [(1977) Biochim. Biophys. Acta 490, 370-384]. Of these subunits, beta' has not previously been reported or characterized...

journal_title：Archives of biochemistry and biophysics

authors： Coates JB,Medeiros JS,Thanh VH,Nielsen NC

更新日期：1985-11-15 00:00:00

abstract：:Ex vivo protein modifications occur within plasma and serum (P/S) samples due to prolonged exposure to the thawed state-which includes temperatures above -30 °C. Herein, the ex vivo glycation of human serum albumin from healthy and diabetic subjects was monitored in P/S samples stored for hours to months at -80 °C, -2...

journal_title：Archives of biochemistry and biophysics

authors： Jeffs JW,Ferdosi S,Yassine HN,Borges CR

更新日期：2017-09-01 00:00:00

abstract：:Glutathione S-transferases (GSTs) of Plasmodium parasites are potential targets for antimalarial drug and vaccine development. We investigated the equilibrium unfolding, functional activity regulation and stability characteristics of the unique GST of Plasmodium vivax (PvGST). Despite high sequence, structural, functi...

journal_title：Archives of biochemistry and biophysics

authors： Tripathi T,Na BK,Sohn WM,Becker K,Bhakuni V

更新日期：2009-07-15 00:00:00

abstract：:Fructose 2,6-bisphosphate inhibited all three fructose-1,6-bisphosphatases from the liver, intestine, and muscle of the mouse. The sensitivity of the liver enzyme to the inhibitor was significantly diminished when Mg2+ was replaced by Mn2+ as the activating cation. Inhibition of the liver enzyme by fructose 2,6-bispho...

journal_title：Archives of biochemistry and biophysics

authors： Mizunuma H,Tashima Y

更新日期：1983-10-01 00:00:00

abstract：:The oxidation of 6-hydroxy-2,2,5,7,8-pentamethylchroman, Trolox C, and alpha-tocopherol by horseradish peroxidase was examined by stopped-flow and ESR experiments. The catalytic intermediate of horseradish peroxidase during the oxidation of vitamin E analogues and vitamin E was invariably Compound II, and rate constan...

journal_title：Archives of biochemistry and biophysics

authors： Nakamura M,Hayashi T

更新日期：1992-12-01 00:00:00

abstract：:A numerical method was applied to a system of differential rate equations describing the monomer-dimer-inhibitor (M-D-I) interaction involving human immunodeficiency virus type 1 protease and a peptidomimetic, competitive inhibitor. Two pairs of progress curves were obtained, one involving the M-D interaction and the ...

journal_title：Archives of biochemistry and biophysics

authors： Morelock MM,Graham ET,Erdman D,Pargellis CA

更新日期：1996-04-15 00:00:00

abstract：:We tested the effects of a series of synthetic oligonucleotides (hybridons) complementary to the 5' noncoding and coding regions of rabbit beta-globin mRNA on endogenous protein synthesis in a rabbit reticulocyte cell-free translation system. With highly purified hybridons inhibition was completely specific for beta-g...

journal_title：Archives of biochemistry and biophysics

authors： Goodchild J,Carroll E 3rd,Greenberg JR

更新日期：1988-06-01 00:00:00

abstract：:Serum acetone in neonates was found to increase from < 20 microM at 20 days gestation to 377 +/- 107 microM 1 day after birth. This increase in acetone occurs concurrently with the initial expression of liver P450IIE1 in rat (Song et al., 1986, J. Biol. Chem. 261:16689-16697). Treating pregnant rats with drinking wate...

journal_title：Archives of biochemistry and biophysics

authors： Casazza JP,Sohn DH,Park KS,Song BJ

更新日期：1994-02-15 00:00:00

abstract：:The changes in retinoid metabolism have been documented in liver cirrhosis. However, the dynamic alterations in levels of this vitamin between circulation and liver during development of the liver cirrhosis are not well understood. The aim of this study was to measure retinoids in the liver and circulation in parallel...

journal_title：Archives of biochemistry and biophysics

authors： Natarajan SK,Thomas S,Ramachandran A,Pulimood AB,Balasubramanian KA

更新日期：2005-11-15 00:00:00

abstract：:Point mutations at position 141 in the murine interleukin-2 (IL-2) sequence have been reported to generate proteins with full antagonist activity on some IL-2-dependent cell lines. To evaluate the potential therapeutic utility of this observation, we have prepared recombinant human IL-2 with a point mutation at the co...

journal_title：Archives of biochemistry and biophysics

authors： Buchli P,Ciardelli T

更新日期：1993-12-01 00:00:00

abstract：:Arachidonate 12-lipoxygenase (12-LO) from porcine leukocytes was expressed in insect cells using a baculovirus expression vector. The recombinant 12-LO was expressed as an N-terminal fusion protein with a 31-amino acid polypeptide carrying a six-histidine tag and an enterokinase cleavage site. Maximal intracellular en...

journal_title：Archives of biochemistry and biophysics

authors： Reddy RG,Yoshimoto T,Yamamoto S,Funk CD,Marnett LJ

更新日期：1994-07-01 00:00:00

abstract：:The serine carboxylesterase from horse serum was characterized by amino acid composition, peptide mapping, molecular and subunit weights, and sequencing of the amino acids around the essential serine residue at the active site. A protocol was developed for using reversed-phase high-performance liquid chromatography as...

journal_title：Archives of biochemistry and biophysics

authors： Torres JL,Rush RS,Main AR

更新日期：1988-11-15 00:00:00

abstract：:Peroxiredoxin 6 (Prdx6) differs from other mammalian peroxiredoxins both in its ability to reduce phospholipid hydroperoxides at neutral pH and in having phospholipase A(2) (PLA(2)) activity that is maximal at acidic pH. We previously showed an active site C47 for peroxidase activity and a catalytic triad S32-H26-D140...

journal_title：Archives of biochemistry and biophysics

authors： Manevich Y,Shuvaeva T,Dodia C,Kazi A,Feinstein SI,Fisher AB

更新日期：2009-05-15 00:00:00

abstract：:The enzyme ketol acid reductoisomerase catalyzes the second common reaction in the biosynthesis of the branched chain amino acids. The reaction is complex as an alkyl migration and a ketone reduction apparently occur as separate steps during the conversion of acetolactate to 2,3-dihydroxy-3-methylbutyrate. This paper ...

journal_title：Archives of biochemistry and biophysics

authors： Mrachko GT,Chunduru SK,Calvo KC

更新日期：1992-05-01 00:00:00

abstract：:Recombinant ethanolamine ammonia-lyase from S. typhimurium has been overexpressed and purified in large quantities by a simple procedure. The molecular weight of the native enzyme is about 480 kDa, and it contains two active sites/molecule as shown by kinetic studies and by titration with CNCbl. Analysis by sodium dod...

journal_title：Archives of biochemistry and biophysics

authors： Faust LP,Babior BM

更新日期：1992-04-01 00:00:00

abstract：:Purple Cu(A) centers are a class of binuclear, mixed-valence copper complexes found in cytochrome c oxidase and nitrous oxide reductase. An engineered Cu(A) protein was formed by replacing a portion of the amino acid sequence that contains three of the ligands to the native type I copper center of Pseudomonas aerugino...

journal_title：Archives of biochemistry and biophysics

authors： Sun D,Wang X,Davidson VL

更新日期：2002-08-01 00:00:00

abstract：:Casein kinase 1 phosphorylated rabbit skeletal muscle glycogen synthase at both seryl and threonyl residues. With glycogen synthase phosphorylated up to 7.5 mol phosphate/mol subunit, about 26% of the phosphate was present in the N-terminal cyanogen bromide fragment (CB1) and 74% in the C-terminal fragment (CB2). Both...

journal_title：Archives of biochemistry and biophysics

authors： Guasch MD,Plana M,Vila J,Itarte E

更新日期：1986-11-01 00:00:00

abstract：:Under certain circumstances, added antioxidants can protect tissues against reoxygenation injury after ischemia. Yet reperfusing blood carries many antioxidants with it. The implications of this "antioxidant paradox" are discussed. ...

journal_title：Archives of biochemistry and biophysics

authors： Gutteridge JM,Halliwell B

更新日期：1990-12-01 00:00:00

abstract：:This study systematically examined the characteristics of specific binding of adult diferric transferrin to its receptor using a Triton X-100 solubilized preparation from human placentas as the receptor source. The following information was obtained. The ionic strength for maximal binding is in the range of 0.1-0.3 M ...

journal_title：Archives of biochemistry and biophysics

authors： Tsunoo H,Sussman HH

更新日期：1983-08-01 00:00:00

abstract：:Activated leukocytes generate the potent oxidants HOCl and HOBr via the formation of H(2)O(2) and the release of peroxidase enzymes (myeloperoxidase, eosinophil peroxidase). HOCl and HOBr are potent microbiocidal agents, but excessive or misplaced production can cause tissue damage and cell lysis. In this study it is ...

journal_title：Archives of biochemistry and biophysics

authors： Hawkins CL,Brown BE,Davies MJ

更新日期：2001-11-15 00:00:00

abstract：:Rat hepatic 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase was purified to homogeneity using agarose-HMG-CoA affinity chromatography. Additional protein was isolated from the affinity column with 0.5 M KCl that demonstrated no HMG-CoA reductase activity, yet comigrated with purified HMG-CoA reductase on sod...

journal_title：Archives of biochemistry and biophysics

authors： Whitehead TR,Vlahcevic ZR,Beg ZH,Hylemon PB

更新日期：1984-05-01 00:00:00

abstract：:Heme and iron are essential to almost all forms of life. The strict maintenance of heme and iron homeostasis is essential to prevent cellular toxicity and the existence of systemic and intracellular regulation is fundamental. Cytosolic heme can be catabolized and detoxified by heme oxygenases (HOs). Interestingly, fre...

journal_title：Archives of biochemistry and biophysics

authors： Yanatori I,Richardson DR,Toyokuni S,Kishi F

更新日期：2019-09-15 00:00:00

abstract：:An inhibitor protein of the catalytic subunit of the cyclic 3',5'-AMP-dependent protein kinase from the nematode Ascaris suum was isolated and characterized. The molecular weight of the inhibitor was estimated as 28,000 by electrophoresis under denaturing conditions and as 30,000 by gel permeation chromatography on Su...

journal_title：Archives of biochemistry and biophysics

authors： Hoffmann R,Jung S,Hofer HW

更新日期：1992-09-01 00:00:00

abstract：:One of the key steps involved in T-cell activation is binding of the tyrosine kinase ZAP-70 via its two SH2 domains to peptide segments termed tyrosine-based activation motifs (ITAM) which are present in three of the T-cell receptor (TCR) subunits. The crystal structure of the ZAP-70 SH2 domains complexed to phosphope...

journal_title：Archives of biochemistry and biophysics

authors： Labadia ME,Jakes S,Grygon CA,Greenwood DJ,Schembri-King J,Lukas SM,Warren TC,Ingraham RH

更新日期：1997-06-01 00:00:00

abstract：:Liver mitochondria were incubated through extended State 3-State 4 transitions (B. Chance and G. N. Williams (1955) J. Biol. Chem. 217, 409-423) in the presence of high concentrations of adenine nucleotides and in presence and absence of a protonophore. In the terminal phase of these transitions (the region of respira...

journal_title：Archives of biochemistry and biophysics

authors： Davis EJ,Davis-van Thienen WI

更新日期：1989-12-01 00:00:00

abstract：:TDP-43 is a nuclear protein whose abnormal aggregates are implicated in ALS and FTLD. Recently, an Asn/Gln rich C-terminal segment of TDP-43 has been shown to produce aggregation in vitro and reproduce most of the protein's pathological hallmarks in cells, but little is known about this segment's structure. Here, CD a...

journal_title：Archives of biochemistry and biophysics

authors： Mompeán M,Buratti E,Guarnaccia C,Brito RM,Chakrabartty A,Baralle FE,Laurents DV

更新日期：2014-03-01 00:00:00

abstract：:Rat transferrin (rTf), containing one complex glycan, and human transferrin (hTf), containing two complex glycans, were treated with peptide:N-glycosidase F (PNGase) under nondenaturing conditions. Apo rTf with a nonfucosylated standard biantennary glycan, but not its diferric counterpart, yielded satisfactory amounts...

journal_title：Archives of biochemistry and biophysics

authors： Regoeczi E,Bolyos M,Chindemi PA

更新日期：1989-02-01 00:00:00

abstract：:Physicochemical and saccharide-binding studies have been performed on Trichosanthes cucumerina seed lectin (TCSL). The agglutination activity of TCSL is highest in the pH range 8.0-11.0, whereas below pH 7.0 it decreases quite rapidly, which is consistent with the involvement of imidazole side chains of His residues, ...

journal_title：Archives of biochemistry and biophysics

authors： Kenoth R,Komath SS,Swamy MJ

更新日期：2003-05-01 00:00:00