Abstract:
:Liver mitochondria were incubated through extended State 3-State 4 transitions (B. Chance and G. N. Williams (1955) J. Biol. Chem. 217, 409-423) in the presence of high concentrations of adenine nucleotides and in presence and absence of a protonophore. In the terminal phase of these transitions (the region of respiratory control), (a) there was a proportional relationship between the phosphorylation potential and membrane potential (delta psi); and (b) the rate of phosphorylation (Jp) was proportionately and inversely related to the back-pressure of delta psi (reflective of proton-motive force (delta p); (c) when phosphorylation was limited by the magnitude of delta psi in the presence of increasing [protonophore], Jp was proportionately and directly related to delta psi. The slopes of these two dependencies (a and c) were approximately equal, but opposite in sign. Protonophore or ADP, added separately, decreased delta psi but the extent of decrease in delta psi by ADP added after increasing amounts of protonophore decreased in a manner proportional to Jp. These data are in all respects consistent with bulk-phase delta p being the central intermediate driving (or suppressing) the phosphorylation reaction.
journal_name
Arch Biochem Biophysjournal_title
Archives of biochemistry and biophysicsauthors
Davis EJ,Davis-van Thienen WIdoi
10.1016/0003-9861(89)90391-3subject
Has Abstractpub_date
1989-12-01 00:00:00pages
449-58issue
2eissn
0003-9861issn
1096-0384pii
0003-9861(89)90391-3journal_volume
275pub_type
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