Abstract：
:According to differences in mobility on SDS-polyacrylamide gel electrophoresis, calpastatins (inhibitor proteins of the calcium-dependent proteinase calpain) are classified into the tissue type (100-120 kDa) and the erythrocyte type (70 kDa), which lacks the amino-terminal domains (domains L and 1). We investigated the molecular diversity of calpastatin in human hematopoietic cells by Western-blot analysis and by the reverse-transcription-polymerase-chain reaction method. While the mononuclear and polymorphonuclear cells in peripheral blood showed the tissue type (110 and 114 kDa), a cell line of erythroid cells (JK-1) showed both the tissue type (110 kDa) and the erythrocyte type (70 kDa) at approximately equal ratios. When the lysate of JK-1 cells was incubated in the presence of ATP, the 110-kDa form was degraded much faster than the 70-kDa form. In human erythrocytes, the 110-kDa form was identified as the tissue type by an antibody recognizing domain L, and this form was also present in addition to the predominant 70-kDA form. JK-1 cells, as well as nucleated cells in peripheral blood, contained calpastatin mRNA with exon-3-deleted. Glioblastoma and fibroblast cell lines expressed the nondeleted calpastatin mRNA in addition to the deletion type, and they showed bands corresponding to 117 kDa as well as 110 and 114 kDa. The 117-kDa band was detectable by an anti-exon 3 peptide antibody. These results suggest that diversity among the tissue type calpastatins is caused by both alternative splicing and post-translational processing whereas the apparent conversion from the tissue type to the erythrocyte type is caused by proteolytic processing.
journal_name
Arch Biochem Biophysjournal_title
Archives of biochemistry and biophysicsauthors
Takano E,Nosaka T,Lee WJ,Nakamura K,Takahashi T,Funaki M,Okada H,Hatanaka M,Maki Mdoi
10.1006/abbi.1993.1294subject
Has Abstractpub_date
1993-06-01 00:00:00pages
349-54issue
2eissn
0003-9861issn
1096-0384pii
S0003-9861(83)71294-4journal_volume
303pub_type
杂志文章abstract：:Gene expression in the obligate intracellular bacterium Chlamydia trachomatis ranges from nil in the infectious EB form to high in the dividing RB form. Little is known about the mechanisms of gene regulation in chlamydiae and only a few promoter sequences have been characterized. The purpose of our study was to exami...
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pub_type： 杂志文章
doi：10.1016/0003-9861(92)90376-8
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pub_type： 杂志文章
doi：10.1006/abbi.1994.1479
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journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
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journal_title：Archives of biochemistry and biophysics
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pub_type： 杂志文章,评审
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journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
doi：10.1016/0003-9861(91)90578-7
更新日期：1991-11-01 00:00:00
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journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章,评审
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journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章,评审
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journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
doi：10.1006/abbi.1994.1406
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journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
doi：10.1006/abbi.2000.1906
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journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
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journal_title：Archives of biochemistry and biophysics
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doi：10.1006/abbi.1994.1013
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更新日期：2005-07-15 00:00:00
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journal_title：Archives of biochemistry and biophysics
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更新日期：1999-01-01 00:00:00
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doi：10.1006/abbi.1994.1134
更新日期：1994-04-01 00:00:00
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journal_title：Archives of biochemistry and biophysics
pub_type： 杂志文章
doi：10.1006/abbi.1994.1274
更新日期：1994-07-01 00:00:00
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journal_title：Archives of biochemistry and biophysics
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journal_title：Archives of biochemistry and biophysics
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更新日期：2009-07-15 00:00:00
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journal_title：Archives of biochemistry and biophysics
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更新日期：2013-09-01 00:00:00
