Abstract:
:A58 is a murine monoclonal antibody raised against the internal dimeric subunit A alpha 1 of the hemocyanin of the scorpion Androctonus australis. When A58 is tested against a range of related hemocyanins, it exhibits cross-reactivity with a limited number of scorpion species. All of them belong to a single Scorpion Family: the Buthidae. We have utilized a bacterial expression-export system in which the variable regions of A58 are expressed as a single-chain variable fragment (scFv). Polymerase chain reaction (PCR) was used for the cloning and modification of the heavy and light variable regions which were assembled into a scFv. After insertion into a vector which contains the pectate lyase signal sequence from Erwinia caratovora for periplasmic expression, a scFv protein was produced in high yield as a soluble and functional protein. The bacterial produced A58 scFv has binding properties similar to those of the original murine antibody. It binds specifically the heterodimeric subunit of Androctonus australis hemocyanin, cross-reacts with only one subunit of each Buthidae investigated, and blocks the recognition of the hemocyanin by antibody A58 in competitive enzyme-linked immunosorbant assay. Previous reports have demonstrated the value of monoclonal antibodies in taxonomical and molecular evolution studies. A58scFv is the first of a new generation of antigen-binding proteins which should prove useful both in taxonomical studies and in the analysis of VH--VL structure-function relationships of antibodies.
journal_name
Arch Biochem Biophysjournal_title
Archives of biochemistry and biophysicsauthors
Billiald P,Motta G,Vaux DJdoi
10.1006/abbi.1995.1185subject
Has Abstractpub_date
1995-03-10 00:00:00pages
429-38issue
2eissn
0003-9861issn
1096-0384pii
S0003-9861(85)71185-Xjournal_volume
317pub_type
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journal_title:Archives of biochemistry and biophysics
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doi:10.1016/j.abb.2011.07.017
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journal_title:Archives of biochemistry and biophysics
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journal_title:Archives of biochemistry and biophysics
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journal_title:Archives of biochemistry and biophysics
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journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
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更新日期:1996-06-01 00:00:00
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journal_title:Archives of biochemistry and biophysics
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更新日期:2018-05-01 00:00:00
abstract::Environmental pollution is a major cause of global mortality and burden of disease. All chemical pollution forms together may be responsible for up to 12 million annual excess deaths as estimated by the Lancet Commission on pollution and health as well as the World Health Organization. Ambient air pollution by particu...
journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章,评审
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更新日期:2020-12-15 00:00:00