Ceratitis capitata brain adenylate cyclase and its membrane environment.


:Adenylate cyclase activation by GTP and octopamine as well as basal activity (in the presence of Mg2+) have been studied as a function of membrane structure in plasma membranes from brain of the dipterous Ceratitis capitata. Benzyl alcohol and lidocaine, but not phenobarbital, inhibited the three activities to the same extent. Triton X-100-solubilized adenylate cyclase was also inhibited by benzyl alcohol and lidocaine, but not by phenobarbital. Results could be explained by an effect on the catalytic unit lipid environment, which would be maintained after solubilization, counteracting the effect of these drugs to facilitate lateral diffusion and coupling of adenylate cyclase components in the lipid bilayer. The observation that the insect adenylate cyclase is relatively insensitive to changes in bulk bilayer fluidity is strengthened by the absence of effect of phenobarbital on enzyme activities. Indeed, this compound was as active as lidocaine or benzyl alcohol in increasing bulk membrane fluidity. The response of C. capitata adenylate cyclase to changes in membrane fluidity is different from that recorded in mammalian systems. This may be functionally important and result from the fact that insects are not warm-blooded.


Arch Biochem Biophys


Guillen A,Haro A,Gavilanes FG,Municio AM




Has Abstract


1991-05-01 00:00:00














  • ΔNp63α/IRF6 interplay activates NOS2 transcription and induces autophagy upon tobacco exposure.

    abstract::Tobacco-induced oxidative stress leads to chronic inflammation and is implicated in the development of many human epithelial cancers, including head and neck cancer. Cigarette smoke exposure was shown to induce the expression of the ΔNp63α and nitric oxide synthase (NOS)-2 in head and neck squamous cell carcinoma cell...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Ratovitski EA

    更新日期:2011-02-15 00:00:00

  • Expression of enzymatically active human granzyme 3 in Escherichia coli for analysis of its substrate specificity.

    abstract::Human granzyme 3 (Gr3) is a serine protease contained in the granules of natural killer cells and cytotoxic T lymphocytes. To elucidate the biochemical and physiological characteristics of Gr3, we attempted to prepare an enzymatically active recombinant human Gr3 without refolding and proteolytic activation. An expres...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Hirata Y,Inagaki H,Shimizu T,Li Q,Nagahara N,Minami M,Kawada T

    更新日期:2006-02-01 00:00:00

  • Retinyl ester hydrolases in retinal pigment epithelium.

    abstract::In bovine retinal pigment epithelium membranes we have found three hydrolases which were active against trans-retinyl palmitate. This was possible by assaying different subcellular fractions as a function of pH in the range 3-9. Detection of these activities has been favored by the use in the enzyme assay of Triton X-...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Gueli MC,Nicotra CM,Pintaudi AM,Paganini A,Pandolfo L,De Leo G,Di Bella MA

    更新日期:1991-08-01 00:00:00

  • MicroRNA-885 regulates the growth and epithelial mesenchymal transition of human liver cancer cells by suppressing tropomodulin 1 expression.

    abstract::MicroRNA-885 (miR-885) has been shown to act as vital regulator of tumorigenesis and its tumor-suppressive role has been investigated in several human cancers. However, the role of miR-885 in regulation of epithelial mesenchymal transition of liver cancer cells yet unknown. This study was undertaken to investigate the...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Feng L,Zhang Y,Yang Q,Guo L,Yang F

    更新日期:2020-10-30 00:00:00

  • Gradual changes in permeability of inner mitochondrial membrane precede the mitochondrial permeability transition.

    abstract::Some compounds are known to induce solute-nonselective permeability of the inner mitochondrial membrane (IMM) in Ca2+-loaded mitochondria. Existing data suggest that this process, following the opening of a mitochondrial permeability transition pore, is preceded by different solute-selective permeable states of IMM. A...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Balakirev MY,Zimmer G

    更新日期:1998-08-01 00:00:00

  • Glycogen synthase turnover and phosphorylation in rat H4IIE hepatoma cells.

    abstract::Glycogen synthase was isolated from rat H4IIE hepatoma cells by the use of specific antibodies. Immunoprecipitates from cells grown in the presence of [35S]methionine contained two 35S-labeled polypeptides, designated GS1 and GS2, separable by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Labeling of both...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Armstrong DJ,Roach PJ

    更新日期:1989-11-15 00:00:00

  • Peroxynitrite signaling in human erythrocytes: synergistic role of hemoglobin oxidation and band 3 tyrosine phosphorylation.

    abstract::Peroxynitrite crosses the red blood cell (RBC) membrane and reacts with hemoglobin (Hb) producing mainly metHb, which is reduced back to ferrousHb by NADH- and NADPH-dependent reductases. Peroxynitrite also induces band 3 (B3) tyrosine phosphorylation, a signaling pathway believed to activate glucose metabolism. This ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章,评审


    authors: Metere A,Iorio E,Pietraforte D,Podo F,Minetti M

    更新日期:2009-04-15 00:00:00

  • Determination of dideoxyosone precursors of AGEs in human lens proteins.

    abstract::Dideoxyosones (DDOs) are intermediates in the synthesis of advanced glycation endproducts (AGEs), such as pentosidine and glucosepane. Although the formation of pentosidine and glucosepane in the human lens has been firmly established, the formation of DDOs has not been demonstrated. The aim of this study was to devel...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Linetsky M,Kaid Johar SR,Meltretter J,Padmanabha S,Parmar T,Vasavada AR,Pischetsrieder M,Nagaraj RH

    更新日期:2011-10-01 00:00:00

  • Regulation of the nitrate reductase level in anacystis nidulans: activity decay under nitrogen stress.

    abstract::The in vivo stability of ferredoxin-nitrate reductase from the cyanobacterium Anacystis nidulans under conditions of inhibited protein synthesis has been studied in nitrate-grown cells. A light-promoted rapid decay in cellular nitrate reductase activity took place in the absence of any added nitrogen source, but not i...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Herrero A,Flores E,Guerrero MG

    更新日期:1984-11-01 00:00:00

  • Cationomycin and monensin partition between serum proteins and erythrocyte membrane: consequences for Na+ and K+ transport and antimalarial activities.

    abstract::The ionophore properties of cationomycin and monensin were studied on human erythrocytes by measuring Na+ influx by 23Na NMR and concomitant K+ efflux by potentiometry in the presence of increasing amounts of serum. Both ion currents (Na+ or K+) decreased linearly with the reciprocal of serum amount. The serum effects...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Gibot S,Jeminet G,Juillard J,Gumila C,Ancelin ML,Vial H,Delort AM

    更新日期:1999-03-15 00:00:00

  • Cloning and expression of rabbit and human brain tryptophan hydroxylase cDNA in Escherichia coli.

    abstract::Rabbit and human brain tryptophan hydroxylase were cloned and expressed in Escherichia coli. Each of the respective cDNAs, including the complete coding sequence of tryptophan hydroxylase, was obtained by reverse transcription of rabbit or human brain mRNA and subcloned into the expression vector pET-3C. The expressed...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Tipper JP,Citron BA,Ribeiro P,Kaufman S

    更新日期:1994-12-01 00:00:00

  • Characterization of the promoter region of the rat neprilysin gene.

    abstract::The neprilysin gene is composed of three distinct 5' noncoding exons which can be joined to the first coding exon to generate multiple mRNA species, all encoding the same protein. Genomic fragments containing upstream sequences of each of these three noncoding exons from the rat neprilysin gene were subcloned in the p...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Li C,Hersh LB

    更新日期:1998-10-01 00:00:00

  • Amino acid sequence and glycosylation of functional unit RtH2-e from Rapana thomasiana (gastropod) hemocyanin.

    abstract::The complete amino acid sequence of Rapana thomasiana hemocyanin functional unit RtH2-e was determined by direct sequencing and matrix-assisted laser desorption ionization mass spectrometry of peptides obtained by cleavage with EndoLysC proteinase, chymotrypsin, and trypsin. The single-polypeptide chain of RtH2-e cons...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Stoeva S,Idakieva K,Betzel C,Genov N,Voelter W

    更新日期:2002-03-15 00:00:00

  • Triton X-100 or octyl glucoside inactivates acyl-CoA:cholesterol acyltransferase 1 by dissociating it from a two-fold dimer to a two-fold monomer.

    abstract::Cholesterol is an important lipid molecule and is needed for all mammalian cells. In various cell types, excess cholesterol is stored as cholesteryl esters; acyl-CoA:cholesterol acyltransferase 1 (ACAT1) plays an essential role in this storage process. ACAT1 is located at the endoplasmic reticulum and has nine transme...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Neumann B,Chang CCY,Chang TY

    更新日期:2019-08-15 00:00:00

  • Regulation and intracellular localization of the biotin holocarboxylase synthetase of 3T3-L1 cells.

    abstract::A quantitative assay has been developed to measure holocarboxylase synthetase activity in cellular extracts. This assay was based on measuring the incorporation of [3H]biotin of high specific activity (4.3 Ci/mmol) into purified rat liver apopyruvate carboxylase. With this assay, holocarboxylase synthetase in 3T3-L1 m...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Chang HI,Cohen ND

    更新日期:1983-08-01 00:00:00

  • Vitamin K-dependent carboxylase: effect of detergent concentrations, vitamin K status, and added protein precursors on activity.

    abstract::Activity of the rat liver microsomal vitamin K-dependent carboxylase has been studied at various concentrations of detergent. The activity which could be solubilized by 0.25% Triton X-100 was low but could be greatly increased if vitamin K-deficient rats were given vitamin K a few minutes before they were killed. At h...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Shah DV,Swanson JC,Suttie JW

    更新日期:1983-04-01 00:00:00

  • Molecular mechanisms of the resistance to hydrogen peroxide of enzymes involved in the calvin cycle from halotolerant Chlamydomonas sp. W80.

    abstract::cDNA clones encoding NADP(+)-glyceraldehyde-3-phosphate dehydrogenase (NADP(+)-GAPDH) and sedoheptulose-1,7-bisphosphatase (SBPase) were isolated and characterized from halotolerant Chlamydomonas sp. W80 (C. W80) cells. The cDNA clone for NADP(+)-GAPDH encoded 369 amino acid residues, preceded by the chloroplast trans...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Tamoi M,Kanaboshi H,Miyasaka H,Shigeoka S

    更新日期:2001-06-15 00:00:00

  • Transport of methotrexate in basolateral membrane vesicles from rat liver.

    abstract::Transport of the antifolate cancer drug methotrexate was studied in vesicles isolated from the basolateral membrane of rat liver. Transport of methotrexate by basolateral membrane vesicles (BLMVs) was mostly via uptake into an osmotically active intravesicular space, with some binding (approximately 9%), as shown by i...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Horne DW,Reed KA

    更新日期:1992-10-01 00:00:00

  • Unusual pseudosubstrate specificity of a novel 3,5-dimethoxyphenol O-methyltransferase cloned from Ruta graveolens L.

    abstract::A cDNA was cloned from Ruta graveolens cells encoding a novel O-methyltransferase (OMT) with high similarity to orcinol or chavicol/eugenol OMTs, but containing a serine-rich N-terminus and a 13 amino acid insertion between motifs IV and V. Expression in Escherichia coli revealed S-adenosyl-l-methionine-dependent OMT ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Burga L,Wellmann F,Lukacin R,Witte S,Schwab W,Schröder J,Matern U

    更新日期:2005-08-01 00:00:00

  • Determination of the mechanism of human malic enzyme with natural and alternate dinucleotides by isotope effects.

    abstract::Human malic enzyme was studied by steady state kinetics, deuterium isotope effects, and 13C isotope effects with both the physiological dinucleotide cofactor and several alternate cofactors. The log V vs pH profile with NAD revealed two pK(a) values too close to be separately determined, but with an average value of 7...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Rishavy MA,Yang Z,Tong L,Cleland WW

    更新日期:2001-12-01 00:00:00

  • Investigation of plasma induced electrical and chemical factors and their contribution processes to plasma gene transfection.

    abstract::This study has been done to know what kind of factors in plasmas and processes on cells induce plasma gene transfection. We evaluated the contribution weight of three groups of the effects and processes, i.e. electrical, chemical and biochemical ones, inducing gene transfection. First, the laser produced plasma (LPP) ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Jinno M,Ikeda Y,Motomura H,Kido Y,Satoh S

    更新日期:2016-09-01 00:00:00

  • Efficient production of recombinant brazzein, a small, heat-stable, sweet-tasting protein of plant origin.

    abstract::Brazzein is a 54-amino-acid sweet-tasting protein first isolated from the fruit of Pentadiplandra brazzeana Baillon found in West Africa. Brazzein, as isolated from the fruit, is 500 times sweeter than sucrose on a weight basis (9500 times sweeter on a per-molecule basis). A minor component of brazzein from fruit, des...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Assadi-Porter FM,Aceti DJ,Cheng H,Markley JL

    更新日期:2000-04-15 00:00:00

  • Iron and phosphate content of rat ferritin heteropolymers.

    abstract::An attempt was made to relate the iron and phosphate content of ferritin to its subunit composition. Ferritins from various tissues were separated according to their subunit composition by anion exchange chromatography and according to their iron content by density-gradient centrifugation. Iron and phosphate contents ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Juan SH,Aust SD

    更新日期:1998-09-15 00:00:00

  • Adenine nucleotide-binding sites on mitochondrial F1-ATPase: studies of the inactive complex formed upon binding ADP at a catalytic site.

    abstract::ADP-induced inhibition of mitochondrial F1-ATPase has been studied. It is shown that in the presence of magnesium and the absence of light, the photoaffinity ADP analog, 2-azido-ADP, induces a reversible inhibition of native F1 that is indistinguishable from that obtained with ADP. Photolysis of the inactive complex r...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Chernyak BV,Cross RL

    更新日期:1992-06-01 00:00:00

  • Phosphofructokinase from mollusc muscle is activated by phosphorylation.

    abstract::Phosphofructokinase was purified from muscle tissue of two different molluscs, edible snails, Helix pomatia (gastropoda), and mussels, Mytilus edulis (bivalvia). Under denaturing conditions, both enzymes had a molecular mass of 82 kDa. In the presence of ATP-Mg2+, the enzymes were rapidly phosphorylated in vitro by th...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Biethinger M,Hoffmann R,Hofer HW

    更新日期:1991-06-01 00:00:00

  • Evidence for a radical mechanism in peroxidase-catalyzed coupling. I. Steady-state experiments with various peroxidases.

    abstract::Biosynthesis of thyroxine in the thyroid gland involves a reaction between two diiodotyrosyl residues within the same molecule of thyroglobulin, a large, thyroid-specific glycoprotein. This reaction, generally referred to as the coupling reaction, is catalyzed in the thyroid by the heme-containing glycoprotein enzyme,...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Taurog A,Dorris M,Doerge DR

    更新日期:1994-11-15 00:00:00

  • 17 beta-estradiol hydroxylation catalyzed by human cytochrome P450 1A1: a comparison of the activities induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in MCF-7 cells with those from heterologous expression of the cDNA.

    abstract::Exposure of MCF-7 breast cancer cells to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) causes an elevated cytochrome P450 content and a marked increase in the microsomal hydroxylation of 17 beta-estradiol (E2) at the C-2, C-4, C-15 alpha, and C-6 alpha positions. In this study we investigated the involvement of cytochrom...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Spink DC,Eugster HP,Lincoln DW 2nd,Schuetz JD,Schuetz EG,Johnson JA,Kaminsky LS,Gierthy JF

    更新日期:1992-03-01 00:00:00

  • Iron-dependent uptake of ascorbate into isolated microsomes.

    abstract::A preliminary study (J.M. Mata, R. Assad, and B. Peterkofsky (1981) Arch. Biochem. Biophys. 206, 93-104) suggested that chick embryo limb bone microsomes took up and concentrated [14C]ascorbate in the presence of cofactors for prolyl hydroxylase. In the present study, we found that the apparent Km for ascorbate in the...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Peterkofsky B,Tschank G,Luedke C

    更新日期:1987-04-01 00:00:00

  • Thioredoxin peroxidase-1 (peroxiredoxin-1) is increased in thioredoxin-1 transfected cells and results in enhanced protection against apoptosis caused by hydrogen peroxide but not by other agents including dexamethasone, etoposide, and doxorubicin.

    abstract::Thioredoxin-1 (Trx-1) is a small redox oncoprotein whose expression is increased in a number of human primary cancers where it is associated with aggressive tumor growth, inhibition of apoptosis and decreased patient survival. We report that Trx-1-transfected MCF-7 human breast cancer cells have increased expression o...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Berggren MI,Husbeck B,Samulitis B,Baker AF,Gallegos A,Powis G

    更新日期:2001-08-01 00:00:00

  • NF kappa B-independent transcriptional induction of the human manganous superoxide dismutase gene.

    abstract::Numerous conditions induce expression of manganese-containing superoxide dismutase (MnSOD) in mammalian cells. The reported inducers of MnSOD are all agents that activate two transcription factors, AP-1 and NF kappa B, but several reports have suggested that MnSOD induction relies solely on NF kappa B. We investigated...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Borrello S,Demple B

    更新日期:1997-12-15 00:00:00